Incidental Mutation 'R7826:Gpr75'
ID 602204
Institutional Source Beutler Lab
Gene Symbol Gpr75
Ensembl Gene ENSMUSG00000043999
Gene Name G protein-coupled receptor 75
Synonyms
MMRRC Submission 045880-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.060) question?
Stock # R7826 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 30835358-30843729 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 30841209 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Histidine to Arginine at position 38 (H38R)
Ref Sequence ENSEMBL: ENSMUSP00000105057 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000109430] [ENSMUST00000203878]
AlphaFold Q6X632
Predicted Effect probably damaging
Transcript: ENSMUST00000109430
AA Change: H38R

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000105057
Gene: ENSMUSG00000043999
AA Change: H38R

DomainStartEndE-ValueType
Pfam:7tm_1 59 376 1.6e-18 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000203878
SMART Domains Protein: ENSMUSP00000144900
Gene: ENSMUSG00000020305

DomainStartEndE-ValueType
low complexity region 20 36 N/A INTRINSIC
ANK 48 77 3.5e-2 SMART
ANK 81 110 8e-3 SMART
ANK 117 146 4.8e-5 SMART
ANK 150 179 1.7e-7 SMART
ANK 184 213 1.8e-4 SMART
ANK 217 246 1.8e-6 SMART
ANK 250 279 1.2e-7 SMART
ANK 285 315 1.1e0 SMART
ANK 318 347 1.2e-3 SMART
ANK 354 385 7.7e-1 SMART
SOCS 493 542 2.8e-4 SMART
SOCS_box 499 541 1.6e-17 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.4%
Validation Efficiency 100% (60/60)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002]
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ajm1 C A 2: 25,468,477 (GRCm39) R478L possibly damaging Het
Arhgap27 T C 11: 103,229,153 (GRCm39) T521A probably benign Het
Bean1 CT C 8: 104,908,664 (GRCm39) probably null Het
Btbd9 A T 17: 30,553,301 (GRCm39) I387N probably benign Het
Ccdc27 C T 4: 154,123,958 (GRCm39) probably null Het
Ccdc88a A T 11: 29,453,563 (GRCm39) H1642L probably benign Het
Cd300c2 C T 11: 114,891,644 (GRCm39) V77I possibly damaging Het
Chrnb2 A T 3: 89,670,550 (GRCm39) S63T probably damaging Het
Dlgap2 C T 8: 14,793,410 (GRCm39) P467L probably benign Het
Dnah5 C T 15: 28,367,958 (GRCm39) T2781M probably damaging Het
Donson T C 16: 91,485,344 (GRCm39) D86G possibly damaging Het
Echs1 T A 7: 139,696,349 (GRCm39) probably benign Het
Epha8 C T 4: 136,663,498 (GRCm39) M483I probably benign Het
Eprs1 T G 1: 185,139,165 (GRCm39) V850G probably damaging Het
Fbxw5 C A 2: 25,392,561 (GRCm39) C114* probably null Het
Gm5160 C T 18: 14,558,129 (GRCm39) R69C probably benign Het
Igsf9 T C 1: 172,319,197 (GRCm39) V230A probably benign Het
Lonp2 C T 8: 87,435,641 (GRCm39) P626S probably damaging Het
Lrrtm1 G T 6: 77,221,095 (GRCm39) probably null Het
Man1a2 T C 3: 100,489,455 (GRCm39) E508G probably damaging Het
Mipep C A 14: 61,039,580 (GRCm39) A203E probably damaging Het
Mrc1 T A 2: 14,299,668 (GRCm39) C753S probably damaging Het
Mthfr A G 4: 148,139,467 (GRCm39) E568G probably benign Het
Naca T A 10: 127,879,479 (GRCm39) probably benign Het
Nek10 G A 14: 14,860,846 (GRCm38) probably null Het
Nf2 T C 11: 4,739,750 (GRCm39) T419A probably benign Het
Npas3 A G 12: 53,878,539 (GRCm39) T164A possibly damaging Het
Nudcd3 T A 11: 6,100,581 (GRCm39) I124F possibly damaging Het
Or8g33 A G 9: 39,337,423 (GRCm39) *315Q probably null Het
Pappa2 A T 1: 158,764,010 (GRCm39) C500* probably null Het
Pcdhb18 T A 18: 37,623,995 (GRCm39) S442T probably damaging Het
Phactr4 T C 4: 132,105,752 (GRCm39) N105D possibly damaging Het
Polr1b C A 2: 128,967,464 (GRCm39) F952L probably damaging Het
Ptprb GAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACT GAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACT 10: 116,119,582 (GRCm39) probably benign Het
Pzp T C 6: 128,464,496 (GRCm39) T1344A probably benign Het
Ranbp9 T C 13: 43,573,097 (GRCm39) I312V possibly damaging Het
Rsf1 A G 7: 97,310,368 (GRCm39) probably benign Het
Slc6a4 A T 11: 76,903,851 (GRCm39) I150L probably benign Het
Smpd5 T C 15: 76,180,496 (GRCm39) S433P probably benign Het
Spats1 C T 17: 45,763,644 (GRCm39) D209N probably damaging Het
Spcs2 C T 7: 99,488,984 (GRCm39) G235R probably damaging Het
Sptlc3 T C 2: 139,389,115 (GRCm39) M165T probably benign Het
Synm T C 7: 67,385,337 (GRCm39) D775G probably damaging Het
Tango6 A G 8: 107,419,245 (GRCm39) D264G probably benign Het
Tbc1d30 A T 10: 121,132,710 (GRCm39) L218H probably damaging Het
Tmem132d A G 5: 127,866,953 (GRCm39) I649T probably damaging Het
Tmem145 T C 7: 25,006,939 (GRCm39) Y114H probably damaging Het
Triml1 A T 8: 43,591,803 (GRCm39) V185E possibly damaging Het
Ttll3 CAAAGTAA CAAAGTAAAGTAA 6: 113,376,118 (GRCm39) probably null Het
Ttn T G 2: 76,715,220 (GRCm39) probably benign Het
Utrn C T 10: 12,277,050 (GRCm39) probably null Het
Uty T C Y: 1,137,716 (GRCm39) K887E possibly damaging Het
Zfand2b T C 1: 75,145,502 (GRCm39) F3L possibly damaging Het
Zfp280d C A 9: 72,219,953 (GRCm39) Q243K possibly damaging Het
Zfp418 T C 7: 7,185,668 (GRCm39) F544L probably benign Het
Other mutations in Gpr75
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00332:Gpr75 APN 11 30,841,590 (GRCm39) missense probably damaging 1.00
IGL00787:Gpr75 APN 11 30,842,290 (GRCm39) missense probably benign 0.00
IGL01102:Gpr75 APN 11 30,841,755 (GRCm39) missense probably benign 0.41
IGL01790:Gpr75 APN 11 30,841,132 (GRCm39) missense probably damaging 0.97
IGL01975:Gpr75 APN 11 30,841,835 (GRCm39) missense probably benign 0.09
IGL02266:Gpr75 APN 11 30,841,977 (GRCm39) missense probably benign 0.01
IGL02338:Gpr75 APN 11 30,841,730 (GRCm39) missense probably benign 0.00
IGL02394:Gpr75 APN 11 30,842,190 (GRCm39) missense possibly damaging 0.92
IGL03208:Gpr75 APN 11 30,842,699 (GRCm39) missense probably damaging 1.00
Thinner UTSW 11 30,841,527 (GRCm39) missense probably damaging 1.00
R0053:Gpr75 UTSW 11 30,842,571 (GRCm39) missense possibly damaging 0.71
R0053:Gpr75 UTSW 11 30,842,571 (GRCm39) missense possibly damaging 0.71
R0449:Gpr75 UTSW 11 30,842,456 (GRCm39) missense probably damaging 0.99
R1289:Gpr75 UTSW 11 30,842,706 (GRCm39) missense probably benign 0.23
R1760:Gpr75 UTSW 11 30,841,527 (GRCm39) missense probably damaging 1.00
R3123:Gpr75 UTSW 11 30,841,709 (GRCm39) missense possibly damaging 0.82
R4669:Gpr75 UTSW 11 30,842,072 (GRCm39) missense probably damaging 1.00
R4704:Gpr75 UTSW 11 30,841,110 (GRCm39) missense probably benign
R4809:Gpr75 UTSW 11 30,842,154 (GRCm39) missense possibly damaging 0.74
R4913:Gpr75 UTSW 11 30,841,808 (GRCm39) missense possibly damaging 0.85
R4982:Gpr75 UTSW 11 30,841,463 (GRCm39) missense possibly damaging 0.96
R4982:Gpr75 UTSW 11 30,841,462 (GRCm39) missense probably damaging 1.00
R5071:Gpr75 UTSW 11 30,842,380 (GRCm39) missense probably damaging 1.00
R5318:Gpr75 UTSW 11 30,842,459 (GRCm39) missense probably benign 0.00
R6019:Gpr75 UTSW 11 30,841,640 (GRCm39) missense probably benign 0.39
R6199:Gpr75 UTSW 11 30,841,527 (GRCm39) missense probably damaging 1.00
R6455:Gpr75 UTSW 11 30,841,529 (GRCm39) missense probably damaging 1.00
R7188:Gpr75 UTSW 11 30,842,687 (GRCm39) missense probably damaging 1.00
R8053:Gpr75 UTSW 11 30,841,559 (GRCm39) missense probably benign 0.25
R8703:Gpr75 UTSW 11 30,841,890 (GRCm39) missense probably damaging 0.99
R8870:Gpr75 UTSW 11 30,841,860 (GRCm39) missense probably benign 0.00
R8930:Gpr75 UTSW 11 30,842,571 (GRCm39) missense possibly damaging 0.71
R8932:Gpr75 UTSW 11 30,842,571 (GRCm39) missense possibly damaging 0.71
Z1088:Gpr75 UTSW 11 30,841,139 (GRCm39) missense probably benign 0.11
Predicted Primers PCR Primer
(F):5'- GAGCTGAGATCCTGACTCTTTTC -3'
(R):5'- TGCTACTGGCTGAGCTGAAG -3'

Sequencing Primer
(F):5'- GAGATCCTGACTCTTTTCCTGCTG -3'
(R):5'- TGAACATGGGGGCTGTGACC -3'
Posted On 2019-12-03