Mutagenetix > Incidental Mutations

Incidental Mutation 'R7827:Olfr303'

602249

Institutional Source

Beutler Lab

Gene Symbol

Olfr303

Ensembl Gene

ENSMUSG00000039608

Gene Name

olfactory receptor 303

Synonyms

GA_x6K02T2NHDJ-9712819-9713778, MOR104-2

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R7827 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

86391810-86397789 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 86394557 bp

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 314 (K314*)

Ref Sequence

ENSEMBL: ENSMUSP00000149419 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053958] [ENSMUST00000215234] [ENSMUST00000215532] [ENSMUST00000215733] [ENSMUST00000216409]

Predicted Effect

probably null
Transcript: ENSMUST00000053958
AA Change: K314*

SMART Domains

Protein: ENSMUSP00000062459
Gene: ENSMUSG00000039608
AA Change: K314*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.2e-52	PFAM
Pfam:7tm_1	41	307	4.9e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215234
AA Change: K314*

Predicted Effect

probably null
Transcript: ENSMUST00000215532
AA Change: K314*

Predicted Effect

probably null
Transcript: ENSMUST00000215733
AA Change: K314*

Predicted Effect

probably null
Transcript: ENSMUST00000216409
AA Change: K314*

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	G	A	1: 71,414,678		probably benign	Het
Abcb1b	T	C	5: 8,837,747	S878P	probably damaging	Het
Adamts20	T	C	15: 94,325,933	R1305G	probably damaging	Het
Adcy2	A	T	13: 68,689,281	C650S	probably damaging	Het
Ahnak	A	T	19: 9,005,344	K1331*	probably null	Het
Alpk1	A	T	3: 127,680,051	S768T	probably benign	Het
Ap4b1	T	G	3: 103,815,082	L124R	probably damaging	Het
Arrdc2	A	T	8: 70,839,394	F3Y	probably damaging	Het
Atp9a	A	G	2: 168,705,194	V155A	probably benign	Het
Bptf	A	G	11: 107,047,187	S87P	probably benign	Het
Carmil1	A	T	13: 24,036,438	M697K	probably benign	Het
Ccdc42	G	A	11: 68,594,196	V32M	probably benign	Het
Ces1d	A	G	8: 93,197,666		probably null	Het
Col4a3bp	A	G	13: 96,617,055	H391R	probably damaging	Het
Dcpp2	T	G	17: 23,900,592	Y127D	probably damaging	Het
Def8	C	A	8: 123,447,321	T7K	probably benign	Het
Dnah8	CGTGTCTTCAATATTTTGTTCCCTTTCCCGTAGGTGCCGTCCTTTGACTTTCCTGTGTCTTCAATATTTTGTTCCCTTTCCCGTAGGTGCCGTCCTTTGACTTTCCTGTGTCTTCAATATTTTGTTCCCTTTCCCGTAGGTGCCGTCCTT	CGTGTCTTCAATATTTTGTTCCCTTTCCCGTAGGTGCCGTCCTTTGACTTTCCTGTGTCTTCAATATTTTGTTCCCTTTCCCGTAGGTGCCGTCCTT	17: 30,760,867		probably null	Het
En2	G	A	5: 28,166,596	G24S	probably benign	Het
Evi2a	T	C	11: 79,527,862		probably benign	Het
Fam117b	A	T	1: 59,913,678	H172L	possibly damaging	Het
Fam169b	A	G	7: 68,304,590	I67M	probably benign	Het
Fbxo40	T	A	16: 36,969,499	E416D	probably damaging	Het
Gja8	T	A	3: 96,920,319	N9I	possibly damaging	Het
Gm5538	A	G	3: 59,743,691	N78S	probably damaging	Het
Gsg1l2	T	A	11: 67,785,271	C154S	probably benign	Het
Hdhd3	C	A	4: 62,499,995		probably benign	Het
Hunk	C	T	16: 90,481,326	P341L	possibly damaging	Het
Ighv8-5	T	C	12: 115,067,638	T94A	possibly damaging	Het
Ints3	T	C	3: 90,424,012	N75D	probably benign	Het
Krt39	T	C	11: 99,519,075	E212G	probably damaging	Het
Ldlrad4	A	G	18: 68,254,424	H269R	probably damaging	Het
Lrrc2	T	A	9: 110,960,981	V102E	possibly damaging	Het
Ltbp2	T	A	12: 84,789,881	I1292L	probably benign	Het
Mad2l1bp	A	T	17: 46,148,067	V179E	possibly damaging	Het
Map3k1	A	G	13: 111,756,129	V864A	probably benign	Het
Mapk8ip2	T	G	15: 89,458,119	V511G	probably damaging	Het
Mknk2	A	C	10: 80,667,187	S434A	probably benign	Het
Mllt11	A	G	3: 95,220,237	F74S	probably damaging	Het
Muc16	G	T	9: 18,595,223	T6412K	possibly damaging	Het
Nek5	C	A	8: 22,083,387	A518S	possibly damaging	Het
Npy1r	T	C	8: 66,704,212	F95L	possibly damaging	Het
Nup98	G	A	7: 102,124,362	T1586I	probably benign	Het
Olfr128	T	A	17: 37,923,677	L37*	probably null	Het
Olfr1477	T	G	19: 13,503,223	N293K	probably damaging	Het
Olfr702	A	G	7: 106,823,725	M267T	probably benign	Het
Olig3	A	T	10: 19,356,770	M48L	probably benign	Het
Pcdhb16	A	G	18: 37,478,851	D288G	possibly damaging	Het
Peg10	GC	GCTCC	6: 4,756,452		probably benign	Het
Phip	T	C	9: 82,908,833	I687V	probably benign	Het
Piezo1	G	A	8: 122,482,920	T2348M	probably damaging	Het
Plod3	A	G	5: 136,989,981	T291A	probably benign	Het
Pmpca	G	A	2: 26,390,132	V89I	possibly damaging	Het
Polr1b	C	A	2: 129,125,544	F952L	probably damaging	Het
Pou6f2	C	A	13: 18,378,507	A81S		Het
Ppl	T	A	16: 5,087,964	E1489V	probably damaging	Het
Ptpdc1	A	G	13: 48,579,788	F792S	probably damaging	Het
Pus1	A	G	5: 110,779,716	Y58H	probably damaging	Het
Rex2	C	A	4: 147,058,090	T345N	possibly damaging	Het
Rnf144a	T	C	12: 26,339,440	M1V	probably null	Het
Siglec15	A	T	18: 78,057,230	C13*	probably null	Het
Skint10	G	A	4: 112,774,806	Q6*	probably null	Het
Slc5a1	T	C	5: 33,146,713	W291R	probably damaging	Het
Snx27	A	G	3: 94,519,059	F378L	probably benign	Het
Strip2	A	T	6: 29,923,929	T81S	probably benign	Het
Tas2r143	A	G	6: 42,400,722	N162S	probably damaging	Het
Tatdn1	T	C	15: 58,904,758	D256G	probably benign	Het
Tbc1d5	A	G	17: 50,782,263	S630P	probably damaging	Het
Tlr11	A	G	14: 50,361,154	D199G	probably benign	Het
Tmed8	C	A	12: 87,200,217		probably benign	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,399,157		probably null	Het
Ttll3	TAA	TAAAGCAA	6: 113,399,162		probably null	Het
U2af2	T	C	7: 5,074,662		probably null	Het
Ywhag	C	T	5: 135,911,540	E67K	probably damaging	Het
Zfp105	A	G	9: 122,930,743	H493R	probably damaging	Het
Zfp426	A	T	9: 20,470,150	C514S	probably damaging	Het
Zfp536	T	A	7: 37,569,688	D101V	probably damaging	Het

Other mutations in Olfr303

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02228:Olfr303	APN	7	86395078	missense	possibly damaging	0.50
IGL02902:Olfr303	APN	7	86394535	utr 3 prime	probably benign
IGL02937:Olfr303	APN	7	86394590	missense	possibly damaging	0.89
BB001:Olfr303	UTSW	7	86394730	missense	probably damaging	1.00
BB011:Olfr303	UTSW	7	86394730	missense	probably damaging	1.00
R1455:Olfr303	UTSW	7	86394595	missense	probably damaging	0.97
R1524:Olfr303	UTSW	7	86394812	missense	probably benign	0.01
R1762:Olfr303	UTSW	7	86395145	missense	probably damaging	0.97
R3014:Olfr303	UTSW	7	86394676	missense	probably benign	0.19
R3027:Olfr303	UTSW	7	86394553	missense	probably benign	0.00
R4073:Olfr303	UTSW	7	86394947	missense	probably damaging	0.98
R4502:Olfr303	UTSW	7	86395277	missense	possibly damaging	0.49
R4503:Olfr303	UTSW	7	86395277	missense	possibly damaging	0.49
R4607:Olfr303	UTSW	7	86394510	splice site	probably null
R4608:Olfr303	UTSW	7	86394510	splice site	probably null
R7134:Olfr303	UTSW	7	86395544	start gained	probably benign
R7298:Olfr303	UTSW	7	86394923	missense	probably damaging	1.00
R7924:Olfr303	UTSW	7	86394730	missense	probably damaging	1.00
R8160:Olfr303	UTSW	7	86395265	missense	possibly damaging	0.61
R8294:Olfr303	UTSW	7	86395279	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGAGCCTGCTTGTAAGAG -3'
(R):5'- AAAACATTCTCAACTTGTGCCTCC -3'

Sequencing Primer
(F):5'- AGCCTGCTTGTAAGAGTCTAATG -3'
(R):5'- ATGGCACCATAATCTTTGCCTATG -3'

Posted On

2019-12-03