Mutagenetix > Incidental Mutation

Incidental Mutation 'RF004:Trav15-2-dv6-2'

602715

Institutional Source

Beutler Lab

Gene Symbol

Trav15-2-dv6-2

Ensembl Gene

ENSMUSG00000076848

Gene Name

T cell receptor alpha variable 15-2-DV6-2

Synonyms

Trav15-2/dv6-2

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

RF004 (G1)

Quality Score

214.458

Status

Not validated

Chromosome

Chromosomal Location

53886882-53887451 bp(+) (GRCm39)

Type of Mutation

small insertion (1 aa in frame mutation)

DNA Base Change (assembly)

AAG to AAGCAG at 53887212 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000100437 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000103660]

AlphaFold

A0A075B650

Predicted Effect

probably benign
Transcript: ENSMUST00000103660

SMART Domains

Protein: ENSMUSP00000100437
Gene: ENSMUSG00000076848

Domain	Start	End	E-Value	Type
IGv	44	120	4.09e-9	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.6%
10x: 99.1%
20x: 97.6%

Validation Efficiency

100% (34/34)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059E24Rik	T	A	19: 21,575,645 (GRCm39)	H126L	probably benign	Het
4930407I10Rik	A	T	15: 81,943,550 (GRCm39)	Q54L	possibly damaging	Het
4930433I11Rik	AACC	A	7: 40,642,479 (GRCm39)		probably benign	Het
Adora2a	A	T	10: 75,168,988 (GRCm39)	T151S	probably benign	Het
Ankhd1	GCGGCG	GCGGCGTCGGCG	18: 36,693,963 (GRCm39)		probably benign	Het
Ankrd36	A	G	11: 5,612,411 (GRCm39)	K1248E	possibly damaging	Het
Anks1b	G	A	10: 89,869,087 (GRCm39)	G49D	probably damaging	Het
Arl11	T	C	14: 61,548,304 (GRCm39)	V38A	probably damaging	Het
Atp2c2	A	T	8: 120,479,561 (GRCm39)	N726Y	probably damaging	Het
Bcat1	T	C	6: 144,953,349 (GRCm39)	K413R	probably benign	Het
Cd244a	C	G	1: 171,405,490 (GRCm39)	Q292E	probably benign	Het
Chp1	T	A	2: 119,411,195 (GRCm39)	D123E	probably damaging	Het
Cpeb4	ACTCT	ACTCTCT	11: 31,877,634 (GRCm39)		probably benign	Het
Ddx6	A	G	9: 44,535,789 (GRCm39)	T173A	possibly damaging	Het
Dlg2	T	A	7: 90,501,885 (GRCm39)	C66S	probably benign	Het
Dnah2	T	A	11: 69,328,013 (GRCm39)	Q3370L	probably benign	Het
Dnmt1	GCACAGTTCCTACCTCGTT	GCACAGTTCCTACCTCGTTTTGGGGGCGGAACACAGTTCCTACCTCGTT	9: 20,821,423 (GRCm39)		probably null	Het
Dop1a	T	C	9: 86,436,244 (GRCm39)	V2420A	probably benign	Het
Gm8369	GTGTGT	GTGTGTATGTGT	19: 11,489,118 (GRCm39)		probably benign	Het
Igkv6-25	TTGACGGA	T	6: 70,192,647 (GRCm39)		probably null	Het
Iqgap1	G	A	7: 80,370,623 (GRCm39)	A1582V	probably benign	Het
Lmnb1	T	C	18: 56,864,046 (GRCm39)	I217T	possibly damaging	Het
Mamld1	CAG	CAGTAG	X: 70,162,437 (GRCm39)		probably null	Het
Map2k2	A	T	10: 80,951,002 (GRCm39)	H149L	probably benign	Het
Med12l	CAG	CAGAAG	3: 59,183,390 (GRCm39)		probably benign	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Nxph2	G	A	2: 23,290,080 (GRCm39)	R144Q	probably damaging	Het
Or2t48	CA	C	11: 58,419,983 (GRCm39)		probably null	Het
Or51f1e	T	TTAG	7: 102,747,516 (GRCm39)		probably null	Het
Or51f1e	GTTAT	GTTATTAT	7: 102,747,512 (GRCm39)			Het
Or51f1e	AT	ATTCT	7: 102,747,515 (GRCm39)		probably benign	Het
Or51q1	TCC	TCCC	7: 103,629,110 (GRCm39)		probably null	Het
Or52n20	A	G	7: 104,320,248 (GRCm39)	E113G	probably damaging	Het
Or8d2	T	C	9: 38,760,114 (GRCm39)	F235L	probably benign	Het
Padi4	A	T	4: 140,487,269 (GRCm39)	V211E	probably damaging	Het
Prdm10	A	C	9: 31,270,422 (GRCm39)	D902A	probably damaging	Het
Prps1l1	A	G	12: 35,035,398 (GRCm39)	D171G	probably damaging	Het
Rasal3	A	T	17: 32,610,081 (GRCm39)	N1035K	probably damaging	Het
Rassf6	GGTCCTGTAGAGCAATGGGGATTC	GGTCCTGTAGAGCAATGGGGATTCTGCATCACTCATTGTCCTGTAGAGCAATGGGGATTC	5: 90,756,778 (GRCm39)		probably benign	Het
Rbm26	A	G	14: 105,388,931 (GRCm39)	V320A	probably damaging	Het
S1pr1	A	T	3: 115,506,536 (GRCm39)	Y19*	probably null	Het
Slc22a16	A	C	10: 40,479,642 (GRCm39)	L571F	possibly damaging	Het
Smarca2	CAGC	CAGCCCAAGC	19: 26,608,420 (GRCm39)		probably benign	Het
Ssx2ip	A	T	3: 146,132,195 (GRCm39)	K219*	probably null	Het
Tsen15	A	G	1: 152,259,470 (GRCm39)	V63A	probably damaging	Het
Ttc21a	A	G	9: 119,795,838 (GRCm39)	Y1224C	probably damaging	Het
Usp54	T	A	14: 20,611,368 (GRCm39)	E1149D	possibly damaging	Het
Vmn2r37	A	T	7: 9,220,686 (GRCm39)	S392R	probably damaging	Het
Wdr97	GAGGAGGA	G	15: 76,247,373 (GRCm39)		probably null	Het
Zfp663	G	T	2: 165,200,363 (GRCm39)	H72Q	probably benign	Het
Zfp683	TGTGG	TGTGGTGG	4: 133,786,185 (GRCm39)		probably benign	Het

Other mutations in Trav15-2-dv6-2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
FR4304:Trav15-2-dv6-2	UTSW	14	53,887,207 (GRCm39)	small insertion	probably benign
FR4548:Trav15-2-dv6-2	UTSW	14	53,887,214 (GRCm39)	small insertion	probably benign
FR4737:Trav15-2-dv6-2	UTSW	14	53,887,214 (GRCm39)	small insertion	probably benign
FR4737:Trav15-2-dv6-2	UTSW	14	53,887,213 (GRCm39)	small insertion	probably benign
FR4976:Trav15-2-dv6-2	UTSW	14	53,887,214 (GRCm39)	small insertion	probably benign
FR4976:Trav15-2-dv6-2	UTSW	14	53,887,211 (GRCm39)	nonsense	probably null
R5966:Trav15-2-dv6-2	UTSW	14	53,887,433 (GRCm39)	missense	probably damaging	1.00
R6113:Trav15-2-dv6-2	UTSW	14	53,887,182 (GRCm39)	missense	probably benign	0.00
R6270:Trav15-2-dv6-2	UTSW	14	53,887,323 (GRCm39)	missense	probably benign	0.00
R7298:Trav15-2-dv6-2	UTSW	14	53,887,242 (GRCm39)	missense	probably benign	0.41
R7528:Trav15-2-dv6-2	UTSW	14	53,887,308 (GRCm39)	missense	probably benign	0.09
R8996:Trav15-2-dv6-2	UTSW	14	53,887,247 (GRCm39)	missense	probably benign	0.00
R9440:Trav15-2-dv6-2	UTSW	14	53,886,932 (GRCm39)	critical splice donor site	probably benign
RF004:Trav15-2-dv6-2	UTSW	14	53,887,211 (GRCm39)	nonsense	probably null
RF004:Trav15-2-dv6-2	UTSW	14	53,887,207 (GRCm39)	small insertion	probably benign
RF005:Trav15-2-dv6-2	UTSW	14	53,887,211 (GRCm39)	small insertion	probably benign
RF005:Trav15-2-dv6-2	UTSW	14	53,887,208 (GRCm39)	small insertion	probably benign
RF005:Trav15-2-dv6-2	UTSW	14	53,887,207 (GRCm39)	small insertion	probably benign
RF007:Trav15-2-dv6-2	UTSW	14	53,887,212 (GRCm39)	small insertion	probably benign
RF007:Trav15-2-dv6-2	UTSW	14	53,887,208 (GRCm39)	small insertion	probably benign
RF007:Trav15-2-dv6-2	UTSW	14	53,887,207 (GRCm39)	small insertion	probably benign
RF026:Trav15-2-dv6-2	UTSW	14	53,887,214 (GRCm39)	small insertion	probably benign

Predicted Primers

PCR Primer
(F):5'- AGAGAAGCCAGCACTCTGTATC -3'
(R):5'- TACGTCCCTGAATCCTCTGG -3'

Sequencing Primer
(F):5'- GAGAAGCCAGCACTCTGTATCTTTAG -3'
(R):5'- CCTGAATCCTCTGGCTGTGAG -3'

Posted On

2019-12-04