Mutagenetix > Incidental Mutation

Incidental Mutation 'RF008:Or10d4c'

602994

Institutional Source

Beutler Lab

Gene Symbol

Or10d4c

Ensembl Gene

ENSMUSG00000059106

Gene Name

olfactory receptor family 10 subfamily D member 4C

Synonyms

GA_x6K02T2PVTD-33343617-33344561, MOR224-5, Olfr961

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.180) question?

Stock #

RF008 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

39558024-39558968 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 39558559 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 179 (M179T)

Ref Sequence

ENSEMBL: ENSMUSP00000151840 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076548] [ENSMUST00000219295]

AlphaFold

Q9EQ84

Predicted Effect

probably benign
Transcript: ENSMUST00000076548
AA Change: M179T

PolyPhen 2 Score 0.092 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000075863
Gene: ENSMUSG00000059106
AA Change: M179T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	1.1e-50	PFAM
Pfam:7tm_1	39	286	4.5e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000219295
AA Change: M179T

PolyPhen 2 Score 0.092 (Sensitivity: 0.93; Specificity: 0.85)

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.7%

Validation Efficiency

100% (45/45)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abi3bp	CCACGACC	CCACGACCACGACC	16: 56,447,952 (GRCm39)		probably benign	Het
Acan	T	G	7: 78,742,148 (GRCm39)	V515G	possibly damaging	Het
Acap3	TGG	TGGACTGCTGCATCCAGG	4: 155,989,555 (GRCm39)		probably benign	Het
AI837181	G	GGCT	19: 5,475,266 (GRCm39)		probably benign	Het
Ankhd1	GGCGGC	GGCGGCAGCGGC	18: 36,693,977 (GRCm39)		probably benign	Het
Anks1b	G	A	10: 89,869,087 (GRCm39)	G49D	probably damaging	Het
Arid1b	GCG	GCGACG	17: 5,045,869 (GRCm39)		probably benign	Het
Arid1b	CGG	CGGAGG	17: 5,045,870 (GRCm39)		probably benign	Het
Begain	G	GCCGCCC	12: 108,999,363 (GRCm39)		probably null	Het
Cacna1e	A	G	1: 154,317,882 (GRCm39)	M1500T	probably damaging	Het
Casp16	C	A	17: 23,774,201 (GRCm39)		probably benign	Het
Ccdc174	T	C	6: 91,876,347 (GRCm39)	S395P	possibly damaging	Het
Ccdc7a	T	G	8: 129,691,434 (GRCm39)	Q396H	probably damaging	Het
Ccdc85c	CC	CCGGC	12: 108,240,887 (GRCm39)		probably benign	Het
Cemip	T	C	7: 83,610,843 (GRCm39)	T704A	probably damaging	Het
Cfap68	C	T	9: 50,677,067 (GRCm39)	R8H	probably benign	Het
Col7a1	C	T	9: 108,793,547 (GRCm39)	P1370S	unknown	Het
Cts8	T	C	13: 61,397,102 (GRCm39)	I273V	probably benign	Het
Cyp2c50	T	A	19: 40,078,268 (GRCm39)	I42N	probably damaging	Het
Dbt	T	G	3: 116,341,717 (GRCm39)	Y439*	probably null	Het
Dennd1b	T	A	1: 138,981,135 (GRCm39)	D116E	probably damaging	Het
Dkk2	T	A	3: 131,883,863 (GRCm39)	H254Q	probably damaging	Het
Dpy30	C	A	17: 74,622,902 (GRCm39)	V27F	possibly damaging	Het
Efhd2	GCCGCC	GCCGCCCCCGCC	4: 141,602,069 (GRCm39)		probably benign	Het
Eif4g3	A	T	4: 137,903,235 (GRCm39)	E1020V	probably damaging	Het
Elmo1	T	A	13: 20,458,706 (GRCm39)	S156T	probably benign	Het
Fancd2os	T	C	6: 113,574,881 (GRCm39)	T42A	probably damaging	Het
Fbrsl1	TGTGC	TGTGCGGGTGTGGGTGC	5: 110,525,984 (GRCm39)		probably benign	Het
Fsip2	T	C	2: 82,808,184 (GRCm39)	V1501A	probably benign	Het
Gabrb2	A	G	11: 42,517,705 (GRCm39)	Y509C	probably damaging	Het
Gm8369	GTGT	GTGTTTGT	19: 11,489,118 (GRCm39)		probably null	Het
Gpr139	T	C	7: 118,744,090 (GRCm39)	D165G	probably benign	Het
Grik2	T	C	10: 49,120,480 (GRCm39)	E603G	probably damaging	Het
H13	G	A	2: 152,511,589 (GRCm39)	E30K	probably damaging	Het
Ifitm10	T	A	7: 141,882,330 (GRCm39)	M147L	unknown	Het
Itpkb	C	T	1: 180,160,887 (GRCm39)	R338W	probably damaging	Het
Kif1b	T	A	4: 149,336,195 (GRCm39)		probably null	Het
Krtap28-10	ACCACAGCCACAGCCACCACAGCCACAGCCACCACAGC	ACCACAGCCACAGCCACCACAGCCACAGCCACCACAGCCACAGCCACCACAGC	1: 83,019,974 (GRCm39)		probably benign	Het
Krtap28-10	AGCCACCACAGC	AGCCACCACAGCCACCGCCACCACAGC	1: 83,020,000 (GRCm39)		probably benign	Het
Krtap28-10	CAGCCA	CAGCCATAGCCA	1: 83,019,849 (GRCm39)		probably benign	Het
Krtap28-10	AGCCAC	AGCCACCGCCAC	1: 83,019,856 (GRCm39)		probably benign	Het
Las1l	AGTGG	AGTGGTGG	X: 94,984,422 (GRCm39)		probably benign	Het
Mical2	T	C	7: 111,922,833 (GRCm39)	Y613H	probably damaging	Het
Mlf2	C	A	6: 124,911,259 (GRCm39)	H91Q	probably benign	Het
Nat9	A	T	11: 115,074,212 (GRCm39)	I152N	probably damaging	Het
Or4a75	A	T	2: 89,447,711 (GRCm39)	I275N	possibly damaging	Het
Pdik1l	CACCAC	CACCACAACCAC	4: 134,006,822 (GRCm39)		probably benign	Het
Phldb2	T	C	16: 45,583,337 (GRCm39)	S1054G	probably damaging	Het
Pkhd1l1	TTTT	TTTTTTTTTTGTTT	15: 44,421,901 (GRCm39)		probably benign	Het
Plcg2	T	A	8: 118,300,263 (GRCm39)		probably null	Het
Polr1has	CACCACCACCACCACCAC	CACCACCACCACCACCACCACAACCACCACCACCACCAC	17: 37,275,946 (GRCm39)		probably benign	Het
Prdm16	T	A	4: 154,426,452 (GRCm39)	R444S	probably damaging	Het
Rgs6	T	C	12: 83,110,223 (GRCm39)	F163L	probably damaging	Het
Rnaseh2a	A	T	8: 85,686,687 (GRCm39)	L154*	probably null	Het
Rpp38	A	G	2: 3,330,072 (GRCm39)	S277P	unknown	Het
Setd1a	GGTAGTGGT	GGTAGTGGTTGTAGTGGT	7: 127,384,486 (GRCm39)		probably benign	Het
Shisa6	CGACAGCAGCAGAG	CG	11: 66,416,749 (GRCm39)		probably benign	Het
Slc27a2	T	A	2: 126,395,175 (GRCm39)	L34Q	possibly damaging	Het
Slc7a6	G	A	8: 106,922,030 (GRCm39)	V386I	probably benign	Het
Spry1	C	T	3: 37,697,264 (GRCm39)	T169I	possibly damaging	Het
Sry	TGCTGCTGCTG	TGCTGCTGCTGCTG	Y: 2,662,826 (GRCm39)		probably benign	Het
Stk24	A	T	14: 121,532,172 (GRCm39)	I275N	probably benign	Het
Strn	TC	TCCGTGCTCCCTTACCCCAGTCCGTGCTCCCTTACCCCAGGC	17: 78,984,716 (GRCm39)		probably null	Het
Tcp11l2	C	A	10: 84,449,388 (GRCm39)	P451Q	probably damaging	Het
Tfeb	AGC	AGCTGC	17: 48,097,027 (GRCm39)		probably benign	Het
Trappc9	TGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCT	TGCTGCTGCTGCTGCGGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCT	15: 72,673,138 (GRCm39)		probably benign	Het
V1ra8	A	G	6: 90,180,591 (GRCm39)	T265A	probably benign	Het
Zfp106	CTCCTGGCAGT	CT	2: 120,355,026 (GRCm39)		probably benign	Het
Zfp612	C	A	8: 110,816,193 (GRCm39)	H467N	probably damaging	Het
Zfp777	A	T	6: 48,018,982 (GRCm39)	Y317*	probably null	Het

Other mutations in Or10d4c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00309:Or10d4c	APN	9	39,558,636 (GRCm39)	missense	probably benign	0.19
IGL01792:Or10d4c	APN	9	39,558,955 (GRCm39)	missense	probably benign	0.07
R0344:Or10d4c	UTSW	9	39,558,646 (GRCm39)	missense	probably damaging	1.00
R0503:Or10d4c	UTSW	9	39,558,772 (GRCm39)	missense	probably damaging	1.00
R0525:Or10d4c	UTSW	9	39,558,767 (GRCm39)	missense	probably damaging	1.00
R0531:Or10d4c	UTSW	9	39,558,168 (GRCm39)	missense	probably benign
R1188:Or10d4c	UTSW	9	39,558,772 (GRCm39)	missense	probably damaging	1.00
R1453:Or10d4c	UTSW	9	39,558,459 (GRCm39)	missense	probably benign	0.01
R2970:Or10d4c	UTSW	9	39,558,195 (GRCm39)	missense	probably damaging	1.00
R3883:Or10d4c	UTSW	9	39,558,420 (GRCm39)	missense	probably benign	0.07
R4423:Or10d4c	UTSW	9	39,558,412 (GRCm39)	missense	probably damaging	1.00
R5129:Or10d4c	UTSW	9	39,558,790 (GRCm39)	missense	probably benign	0.03
R6148:Or10d4c	UTSW	9	39,558,555 (GRCm39)	missense	probably damaging	1.00
R6738:Or10d4c	UTSW	9	39,557,957 (GRCm39)	start gained	probably benign
R6778:Or10d4c	UTSW	9	39,558,043 (GRCm39)	missense	probably damaging	1.00
R7194:Or10d4c	UTSW	9	39,558,387 (GRCm39)	missense	probably benign	0.15
R7545:Or10d4c	UTSW	9	39,558,403 (GRCm39)	missense	probably damaging	0.97
R8547:Or10d4c	UTSW	9	39,558,798 (GRCm39)	nonsense	probably null
R8956:Or10d4c	UTSW	9	39,558,496 (GRCm39)	missense	probably damaging	1.00
R9081:Or10d4c	UTSW	9	39,558,196 (GRCm39)	missense	probably damaging	1.00
R9098:Or10d4c	UTSW	9	39,558,379 (GRCm39)	missense	probably damaging	1.00
R9734:Or10d4c	UTSW	9	39,558,202 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCCTATGACCGCTTTGTTG -3'
(R):5'- GACACACATGATGGCTGTGAGG -3'

Sequencing Primer
(F):5'- ATGACCGCTTTGTTGCCATATG -3'
(R):5'- TGCAGGTGGAGAACGCTCTC -3'

Posted On

2019-12-04