Mutagenetix > Incidental Mutation

Incidental Mutation 'RF013:Col6a5'

603346

Institutional Source

Beutler Lab

Gene Symbol

Col6a5

Ensembl Gene

ENSMUSG00000091345

Gene Name

collagen, type VI, alpha 5

Synonyms

Gm7455, Col6a5

Accession Numbers

Essential gene?

Probably essential (E-score: 0.825) question?

Stock #

RF013 (G1)

Quality Score

127.461

Status

Not validated

Chromosome

Chromosomal Location

105856078-105960643 bp(-) (GRCm38)

Type of Mutation

frame shift

DNA Base Change (assembly)

GCAGTC to GCAGTCTCCAGTC at 105878597 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000139398 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165165] [ENSMUST00000190193]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000165165

SMART Domains

Protein: ENSMUSP00000131146
Gene: ENSMUSG00000091345

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
VWA	28	200	1.8e-24	SMART
low complexity region	222	248	N/A	INTRINSIC
VWA	266	439	2.23e-20	SMART
VWA	472	649	6.84e-39	SMART
VWA	658	834	1.52e-45	SMART
VWA	844	1024	2.44e-44	SMART
VWA	1035	1208	2.95e-20	SMART
Pfam:Collagen	1425	1478	3.3e-8	PFAM
low complexity region	1493	1508	N/A	INTRINSIC
low complexity region	1535	1552	N/A	INTRINSIC
Pfam:Collagen	1555	1616	9.6e-10	PFAM
low complexity region	1711	1730	N/A	INTRINSIC
low complexity region	1739	1757	N/A	INTRINSIC
VWA	1788	1964	1.99e-17	SMART
VWA	1994	2173	5.98e-21	SMART
VWA	2319	2513	4.4e-19	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000190193

SMART Domains

Protein: ENSMUSP00000139398
Gene: ENSMUSG00000091345

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
VWA	28	200	1.1e-26	SMART
low complexity region	222	248	N/A	INTRINSIC
VWA	266	439	1.4e-22	SMART
VWA	472	649	4.4e-41	SMART
VWA	658	834	9.5e-48	SMART
VWA	844	1024	1.6e-46	SMART
VWA	1035	1208	1.9e-22	SMART
Pfam:Collagen	1425	1478	1.2e-6	PFAM
Pfam:Collagen	1457	1530	5.9e-6	PFAM
low complexity region	1535	1552	N/A	INTRINSIC
Pfam:Collagen	1555	1616	3.6e-8	PFAM
Pfam:Collagen	1631	1691	8.4e-6	PFAM
Pfam:Collagen	1706	1764	6.6e-6	PFAM
VWA	1788	1964	1.2e-19	SMART
VWA	1994	2173	3.7e-23	SMART
VWA	2319	2513	2.8e-21	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the collagen superfamily of proteins. The encoded protein contains multiple von Willebrand factor A-like domains and may interact with the alpha 1 and alpha 2 chains of collagen VI to form the complete collagen VI trimer. Polymorphisms in this gene may be linked to dermal phenotypes, such as eczema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700019N19Rik	A	G	19: 58,789,294 (GRCm38)	F28S	probably damaging	Het
4930435E12Rik	T	C	16: 38,828,001 (GRCm38)	T249A	probably benign	Het
4932438A13Rik	TTAT	TTATTATTATTATTAGTAT	3: 37,050,757 (GRCm38)		probably benign	Het
Acap3	CCTGGGCTGCTG	CCTGGGCTGCTGCATACTGGGCTGCTG	4: 155,905,096 (GRCm38)		probably benign	Het
Adamts9	A	G	6: 92,943,145 (GRCm38)	V4A	possibly damaging	Het
AI837181	GGC	GGCTGC	19: 5,425,232 (GRCm38)		probably benign	Het
Alk	A	G	17: 71,895,936 (GRCm38)	Y1135H	probably damaging	Het
Ankhd1	CGGCGG	CGGCGGAGGCGG	18: 36,560,926 (GRCm38)		probably benign	Het
Ano3	A	C	2: 110,697,036 (GRCm38)	L609R	probably benign	Het
Bicc1	A	G	10: 70,935,830 (GRCm38)		probably null	Het
Card6	T	C	15: 5,100,142 (GRCm38)	I591V	probably benign	Het
Ccdc18	A	G	5: 108,220,716 (GRCm38)	N1235D	probably benign	Het
Cd109	TTAT	TTATTTATTTATCTAT	9: 78,712,531 (GRCm38)		probably benign	Het
Cnpy3	CCT	CCTGCT	17: 46,736,744 (GRCm38)		probably benign	Het
Cyb5r4	GACACACTGCCCAGGGA	GACACACTGCCCAGGGATGTGACACACACACTGCCCAGGGA	9: 87,040,432 (GRCm38)		probably benign	Het
Cyp8b1	A	T	9: 121,915,495 (GRCm38)	M257K	possibly damaging	Het
Dbf4	A	T	5: 8,397,985 (GRCm38)	H408Q	possibly damaging	Het
Defb22	TTGCGGCA	TTGCGGCAGAGCTGGCCTGTGCGGCA	2: 152,485,831 (GRCm38)		probably benign	Het
Ercc6l2	A	T	13: 63,853,017 (GRCm38)	T417S	probably benign	Het
Exd2	AGCCACAG	A	12: 80,475,932 (GRCm38)		probably null	Het
Fam171b	GC	GCAGCATC	2: 83,812,895 (GRCm38)		probably benign	Het
Fam71e1	CCTGGGTCTGAGGGAGGA	CCTGGGTCTGAGGGAGGACGGCTGGATCCTGGATCACTGGGTCTGAGGGAGGA	7: 44,500,520 (GRCm38)		probably null	Het
Flvcr2	T	A	12: 85,747,186 (GRCm38)	L112Q	probably damaging	Het
Flywch1	GTG	GTGGGGGGAGGCTACGTACTCACCCACTCCTTTTG	17: 23,762,175 (GRCm38)		probably null	Het
Gabre	TCAGGCTCAGGCT	TCAGGCTCAGGCTCAGGCT	X: 72,270,416 (GRCm38)		probably benign	Het
Gm4884	C	A	7: 41,040,809 (GRCm38)	P43Q	probably damaging	Het
Gm6588	T	A	5: 112,450,071 (GRCm38)	N161K	probably benign	Het
Grm8	A	G	6: 27,363,780 (GRCm38)	W579R	probably damaging	Het
Hsdl2	AG	AGCAGCAGCCACAGCTGCCG	4: 59,610,657 (GRCm38)		probably benign	Het
Ivl	CTGCTGCTGCTGCTGT	C	3: 92,572,343 (GRCm38)		probably benign	Het
Kif18b	T	C	11: 102,912,366 (GRCm38)	D506G	probably benign	Het
Krtap28-10	AGCCAC	AGCCACGGCCAC	1: 83,042,135 (GRCm38)		probably benign	Het
Krtap28-10	GCCACAGCCACCACA	GCCACAGCCACCACATCCACAGCCACCACA	1: 83,042,274 (GRCm38)		probably benign	Het
Lama1	C	A	17: 67,781,062 (GRCm38)	S1558R		Het
Lcmt1	C	CCGCGGGGCTT	7: 123,369,836 (GRCm38)		probably null	Het
Lmna	A	G	3: 88,484,054 (GRCm38)	V494A	probably benign	Het
Mapk6	CCAC	CCACCTCAC	9: 75,388,260 (GRCm38)		probably null	Het
Mboat7	T	A	7: 3,691,857 (GRCm38)	H52L	probably damaging	Het
Med12l	CAG	CAGAAG	3: 59,275,966 (GRCm38)		probably benign	Het
Morc2a	T	A	11: 3,676,191 (GRCm38)	M225K	probably benign	Het
Mpdz	G	A	4: 81,293,592 (GRCm38)	A1566V	possibly damaging	Het
Mpi	T	C	9: 57,548,641 (GRCm38)	D186G	probably benign	Het
Mtmr12	C	A	15: 12,261,898 (GRCm38)	N386K	probably damaging	Het
Myh3	ATTAC	ATTACTTAC	11: 67,086,356 (GRCm38)		probably null	Het
Myo10	T	A	15: 25,799,479 (GRCm38)	M1376K	probably damaging	Het
Nbas	C	T	12: 13,279,408 (GRCm38)	T118I	possibly damaging	Het
Nedd4l	C	T	18: 65,209,680 (GRCm38)	R755C	probably damaging	Het
Nefh	GACTTGGCCTCACCTGGG	GACTTGGCCTCACCTGGGTACTTGGCCTCACCTGGG	11: 4,941,032 (GRCm38)		probably benign	Het
Numa1	T	C	7: 101,999,780 (GRCm38)	L906P	probably damaging	Het
Olfr750	G	A	14: 51,071,012 (GRCm38)	A127V	probably damaging	Het
Olfr871	T	C	9: 20,212,894 (GRCm38)	S182P	probably benign	Het
Otop2	G	T	11: 115,323,666 (GRCm38)	R83L	probably benign	Het
Pmm1	T	A	15: 81,957,813 (GRCm38)	Q62L	probably damaging	Het
Pramef25	C	G	4: 143,948,908 (GRCm38)	Q449H	probably damaging	Het
Ptprj	A	T	2: 90,471,170 (GRCm38)	L206*	probably null	Het
Rassf6	TC	TCTGCCTCACTCATGGTCCTGTAGAGCATTGGGGATCC	5: 90,608,941 (GRCm38)		probably benign	Het
Rps19	A	AGAAAAT	7: 24,889,180 (GRCm38)		probably benign	Het
Rsrp1	T	A	4: 134,923,955 (GRCm38)	V10E	unknown	Het
Sh2d6	C	T	6: 72,516,388 (GRCm38)		probably null	Het
Six4	TG	T	12: 73,103,582 (GRCm38)		probably null	Het
Slc6a15	T	A	10: 103,400,216 (GRCm38)	V264D	probably damaging	Het
Snapc5	ATGGAAGAAGAGG	A	9: 64,182,211 (GRCm38)		probably benign	Het
Sost	A	T	11: 101,964,132 (GRCm38)	I117N	probably damaging	Het
Tbc1d22a	AGGTGTGTG	A	15: 86,299,774 (GRCm38)		probably null	Het
Tcaf1	C	T	6: 42,679,173 (GRCm38)	V290I	probably benign	Het
Tcof1	GCA	GCACCA	18: 60,835,743 (GRCm38)		probably benign	Het
Tgfbr1	A	G	4: 47,353,354 (GRCm38)	I15V	unknown	Het
Tmem241	A	T	18: 11,983,561 (GRCm38)	L288Q	probably damaging	Het
Tnfrsf13b	T	G	11: 61,141,444 (GRCm38)	V100G	probably benign	Het
Trim66	A	G	7: 109,460,753 (GRCm38)	S809P	probably damaging	Het
Tubb4a	C	G	17: 57,087,464 (GRCm38)	G17A	possibly damaging	Het
Txndc16	A	G	14: 45,169,338 (GRCm38)	V220A	probably benign	Het
Zan	T	A	5: 137,391,720 (GRCm38)	Q4830L	unknown	Het

Other mutations in Col6a5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00232:Col6a5	APN	9	105,882,683 (GRCm38)	missense	probably damaging	1.00
IGL01462:Col6a5	APN	9	105,946,075 (GRCm38)	missense	unknown
IGL01530:Col6a5	APN	9	105,915,186 (GRCm38)	splice site	probably benign
IGL01717:Col6a5	APN	9	105,940,273 (GRCm38)	missense	unknown
IGL01859:Col6a5	APN	9	105,930,961 (GRCm38)	nonsense	probably null
IGL01945:Col6a5	APN	9	105,928,290 (GRCm38)	missense	unknown
IGL01985:Col6a5	APN	9	105,937,283 (GRCm38)	missense	unknown
IGL02128:Col6a5	APN	9	105,939,894 (GRCm38)	missense	unknown
IGL02170:Col6a5	APN	9	105,928,422 (GRCm38)	missense	unknown
IGL02224:Col6a5	APN	9	105,864,335 (GRCm38)	missense	probably damaging	1.00
IGL02246:Col6a5	APN	9	105,911,107 (GRCm38)	nonsense	probably null
IGL02304:Col6a5	APN	9	105,928,414 (GRCm38)	missense	unknown
IGL02338:Col6a5	APN	9	105,878,630 (GRCm38)	missense	probably damaging	1.00
IGL02375:Col6a5	APN	9	105,906,113 (GRCm38)	missense	unknown
IGL02660:Col6a5	APN	9	105,936,886 (GRCm38)	missense	unknown
IGL02829:Col6a5	APN	9	105,934,307 (GRCm38)	missense	unknown
IGL02882:Col6a5	APN	9	105,934,321 (GRCm38)	missense	unknown
IGL02973:Col6a5	APN	9	105,925,821 (GRCm38)	missense	unknown
IGL03089:Col6a5	APN	9	105,933,839 (GRCm38)	missense	unknown
IGL03100:Col6a5	APN	9	105,937,313 (GRCm38)	missense	unknown
IGL03257:Col6a5	APN	9	105,881,873 (GRCm38)	missense	possibly damaging	0.95
FR4340:Col6a5	UTSW	9	105,934,174 (GRCm38)	missense	unknown
FR4342:Col6a5	UTSW	9	105,934,174 (GRCm38)	missense	unknown
FR4589:Col6a5	UTSW	9	105,934,174 (GRCm38)	missense	unknown
PIT4131001:Col6a5	UTSW	9	105,881,914 (GRCm38)	missense	probably damaging	0.98
R0147:Col6a5	UTSW	9	105,925,794 (GRCm38)	missense	unknown
R0549:Col6a5	UTSW	9	105,904,579 (GRCm38)	splice site	probably benign
R0622:Col6a5	UTSW	9	105,925,852 (GRCm38)	missense	unknown
R0628:Col6a5	UTSW	9	105,912,450 (GRCm38)	splice site	probably null
R0635:Col6a5	UTSW	9	105,928,606 (GRCm38)	missense	unknown
R0644:Col6a5	UTSW	9	105,948,324 (GRCm38)	critical splice donor site	probably null
R0828:Col6a5	UTSW	9	105,862,064 (GRCm38)	critical splice acceptor site	probably null
R0972:Col6a5	UTSW	9	105,940,285 (GRCm38)	missense	unknown
R1065:Col6a5	UTSW	9	105,881,783 (GRCm38)	missense	probably damaging	0.99
R1142:Col6a5	UTSW	9	105,934,317 (GRCm38)	missense	unknown
R1169:Col6a5	UTSW	9	105,896,974 (GRCm38)	splice site	probably null
R1522:Col6a5	UTSW	9	105,939,994 (GRCm38)	missense	unknown
R1646:Col6a5	UTSW	9	105,862,749 (GRCm38)	nonsense	probably null
R1719:Col6a5	UTSW	9	105,931,293 (GRCm38)	missense	unknown
R1759:Col6a5	UTSW	9	105,930,846 (GRCm38)	missense	unknown
R1780:Col6a5	UTSW	9	105,936,878 (GRCm38)	missense	unknown
R1812:Col6a5	UTSW	9	105,928,054 (GRCm38)	missense	unknown
R1838:Col6a5	UTSW	9	105,864,833 (GRCm38)	missense	probably benign	0.28
R1839:Col6a5	UTSW	9	105,864,833 (GRCm38)	missense	probably benign	0.28
R1863:Col6a5	UTSW	9	105,940,201 (GRCm38)	missense	unknown
R1900:Col6a5	UTSW	9	105,931,213 (GRCm38)	missense	unknown
R1951:Col6a5	UTSW	9	105,936,957 (GRCm38)	missense	unknown
R2024:Col6a5	UTSW	9	105,936,994 (GRCm38)	missense	unknown
R2126:Col6a5	UTSW	9	105,945,600 (GRCm38)	missense	unknown
R2319:Col6a5	UTSW	9	105,937,218 (GRCm38)	missense	unknown
R2344:Col6a5	UTSW	9	105,928,537 (GRCm38)	missense	unknown
R2483:Col6a5	UTSW	9	105,864,148 (GRCm38)	missense	probably damaging	1.00
R3176:Col6a5	UTSW	9	105,911,107 (GRCm38)	nonsense	probably null
R3276:Col6a5	UTSW	9	105,911,107 (GRCm38)	nonsense	probably null
R3438:Col6a5	UTSW	9	105,875,792 (GRCm38)	missense	possibly damaging	0.88
R3791:Col6a5	UTSW	9	105,864,669 (GRCm38)	missense	probably damaging	0.99
R3840:Col6a5	UTSW	9	105,928,611 (GRCm38)	missense	unknown
R3886:Col6a5	UTSW	9	105,930,930 (GRCm38)	missense	unknown
R3941:Col6a5	UTSW	9	105,939,834 (GRCm38)	missense	unknown
R4194:Col6a5	UTSW	9	105,945,914 (GRCm38)	missense	unknown
R4399:Col6a5	UTSW	9	105,888,965 (GRCm38)	missense	possibly damaging	0.75
R4421:Col6a5	UTSW	9	105,928,473 (GRCm38)	missense	unknown
R4450:Col6a5	UTSW	9	105,904,521 (GRCm38)	missense	unknown
R4491:Col6a5	UTSW	9	105,940,012 (GRCm38)	missense	unknown
R4582:Col6a5	UTSW	9	105,862,764 (GRCm38)	missense	probably benign	0.17
R4693:Col6a5	UTSW	9	105,937,172 (GRCm38)	missense	unknown
R4787:Col6a5	UTSW	9	105,931,081 (GRCm38)	missense	unknown
R4789:Col6a5	UTSW	9	105,937,335 (GRCm38)	missense	unknown
R4791:Col6a5	UTSW	9	105,930,784 (GRCm38)	missense	unknown
R4792:Col6a5	UTSW	9	105,930,784 (GRCm38)	missense	unknown
R4817:Col6a5	UTSW	9	105,934,298 (GRCm38)	missense	unknown
R4854:Col6a5	UTSW	9	105,898,751 (GRCm38)	missense	probably benign	0.18
R4927:Col6a5	UTSW	9	105,933,964 (GRCm38)	missense	unknown
R4969:Col6a5	UTSW	9	105,864,607 (GRCm38)	missense	probably damaging	1.00
R5037:Col6a5	UTSW	9	105,928,138 (GRCm38)	missense	unknown
R5118:Col6a5	UTSW	9	105,937,005 (GRCm38)	missense	unknown
R5144:Col6a5	UTSW	9	105,889,283 (GRCm38)	missense	probably damaging	1.00
R5145:Col6a5	UTSW	9	105,934,245 (GRCm38)	missense	unknown
R5160:Col6a5	UTSW	9	105,931,009 (GRCm38)	missense	unknown
R5182:Col6a5	UTSW	9	105,857,332 (GRCm38)	nonsense	probably null
R5234:Col6a5	UTSW	9	105,864,205 (GRCm38)	missense	probably damaging	1.00
R5252:Col6a5	UTSW	9	105,940,290 (GRCm38)	missense	unknown
R5290:Col6a5	UTSW	9	105,946,083 (GRCm38)	missense	unknown
R5313:Col6a5	UTSW	9	105,945,544 (GRCm38)	missense	unknown
R5321:Col6a5	UTSW	9	105,928,465 (GRCm38)	missense	unknown
R5466:Col6a5	UTSW	9	105,931,083 (GRCm38)	missense	unknown
R5540:Col6a5	UTSW	9	105,862,776 (GRCm38)	missense	probably benign	0.44
R5669:Col6a5	UTSW	9	105,925,998 (GRCm38)	missense	unknown
R5789:Col6a5	UTSW	9	105,864,608 (GRCm38)	missense	possibly damaging	0.91
R5801:Col6a5	UTSW	9	105,948,367 (GRCm38)	missense	unknown
R5827:Col6a5	UTSW	9	105,928,120 (GRCm38)	nonsense	probably null
R5839:Col6a5	UTSW	9	105,945,393 (GRCm38)	critical splice donor site	probably null
R5908:Col6a5	UTSW	9	105,862,801 (GRCm38)	missense	possibly damaging	0.88
R5970:Col6a5	UTSW	9	105,945,847 (GRCm38)	missense	unknown
R6045:Col6a5	UTSW	9	105,925,918 (GRCm38)	missense	unknown
R6107:Col6a5	UTSW	9	105,892,272 (GRCm38)	nonsense	probably null
R6168:Col6a5	UTSW	9	105,875,787 (GRCm38)	critical splice donor site	probably null
R6315:Col6a5	UTSW	9	105,881,970 (GRCm38)	missense	probably damaging	1.00
R6317:Col6a5	UTSW	9	105,889,067 (GRCm38)	missense	probably damaging	1.00
R6414:Col6a5	UTSW	9	105,892,266 (GRCm38)	splice site	probably null
R6434:Col6a5	UTSW	9	105,937,345 (GRCm38)	missense	unknown
R6456:Col6a5	UTSW	9	105,945,477 (GRCm38)	missense	unknown
R6698:Col6a5	UTSW	9	105,934,175 (GRCm38)	missense	unknown
R6876:Col6a5	UTSW	9	105,937,307 (GRCm38)	missense	unknown
R6882:Col6a5	UTSW	9	105,940,270 (GRCm38)	nonsense	probably null
R6928:Col6a5	UTSW	9	105,939,919 (GRCm38)	missense	unknown
R7024:Col6a5	UTSW	9	105,912,475 (GRCm38)	nonsense	probably null
R7038:Col6a5	UTSW	9	105,945,738 (GRCm38)	missense	unknown
R7082:Col6a5	UTSW	9	105,931,239 (GRCm38)	missense	unknown
R7158:Col6a5	UTSW	9	105,864,208 (GRCm38)	missense	possibly damaging	0.90
R7211:Col6a5	UTSW	9	105,928,164 (GRCm38)	missense	unknown
R7431:Col6a5	UTSW	9	105,928,269 (GRCm38)	missense	unknown
R7440:Col6a5	UTSW	9	105,881,431 (GRCm38)	nonsense	probably null
R7502:Col6a5	UTSW	9	105,875,876 (GRCm38)	missense	probably benign	0.05
R7577:Col6a5	UTSW	9	105,864,688 (GRCm38)	nonsense	probably null
R7582:Col6a5	UTSW	9	105,945,426 (GRCm38)	missense	unknown
R7641:Col6a5	UTSW	9	105,881,426 (GRCm38)	nonsense	probably null
R7762:Col6a5	UTSW	9	105,931,324 (GRCm38)	missense	unknown
R7793:Col6a5	UTSW	9	105,898,735 (GRCm38)	missense	probably damaging	1.00
R7821:Col6a5	UTSW	9	105,864,259 (GRCm38)	missense	probably damaging	1.00
R7848:Col6a5	UTSW	9	105,928,186 (GRCm38)	missense	unknown
R7897:Col6a5	UTSW	9	105,889,183 (GRCm38)	missense	possibly damaging	0.96
R7904:Col6a5	UTSW	9	105,928,521 (GRCm38)	missense	unknown
R7960:Col6a5	UTSW	9	105,945,850 (GRCm38)	missense	unknown
R8015:Col6a5	UTSW	9	105,881,741 (GRCm38)	missense	possibly damaging	0.65
R8100:Col6a5	UTSW	9	105,878,640 (GRCm38)	missense	probably damaging	1.00
R8131:Col6a5	UTSW	9	105,901,616 (GRCm38)	missense	unknown
R8418:Col6a5	UTSW	9	105,878,622 (GRCm38)	missense	probably damaging	1.00
R8425:Col6a5	UTSW	9	105,945,957 (GRCm38)	missense	unknown
R8678:Col6a5	UTSW	9	105,934,352 (GRCm38)	missense	unknown
R8690:Col6a5	UTSW	9	105,882,597 (GRCm38)	missense	probably damaging	0.97
R8847:Col6a5	UTSW	9	105,864,273 (GRCm38)	missense	possibly damaging	0.81
R8946:Col6a5	UTSW	9	105,945,634 (GRCm38)	missense	unknown
R8947:Col6a5	UTSW	9	105,945,634 (GRCm38)	missense	unknown
R8949:Col6a5	UTSW	9	105,945,634 (GRCm38)	missense	unknown
R8950:Col6a5	UTSW	9	105,945,634 (GRCm38)	missense	unknown
R9089:Col6a5	UTSW	9	105,888,943 (GRCm38)	missense	probably damaging	1.00
R9118:Col6a5	UTSW	9	105,878,654 (GRCm38)	splice site	probably benign
R9169:Col6a5	UTSW	9	105,945,397 (GRCm38)	missense	unknown
R9177:Col6a5	UTSW	9	105,930,953 (GRCm38)	missense	unknown
R9180:Col6a5	UTSW	9	105,861,979 (GRCm38)	missense	probably damaging	0.99
R9205:Col6a5	UTSW	9	105,878,638 (GRCm38)	missense	probably damaging	1.00
R9214:Col6a5	UTSW	9	105,881,741 (GRCm38)	missense	possibly damaging	0.65
R9224:Col6a5	UTSW	9	105,937,395 (GRCm38)	missense	unknown
R9279:Col6a5	UTSW	9	105,881,777 (GRCm38)	missense	probably damaging	1.00
R9383:Col6a5	UTSW	9	105,925,911 (GRCm38)	missense	unknown
R9427:Col6a5	UTSW	9	105,939,793 (GRCm38)	missense	unknown
R9488:Col6a5	UTSW	9	105,864,589 (GRCm38)	missense	probably damaging	1.00
R9494:Col6a5	UTSW	9	105,945,533 (GRCm38)	missense	unknown
R9659:Col6a5	UTSW	9	105,933,835 (GRCm38)	missense	unknown
R9749:Col6a5	UTSW	9	105,861,991 (GRCm38)	missense	probably damaging	0.98
X0054:Col6a5	UTSW	9	105,915,158 (GRCm38)	missense	unknown
X0058:Col6a5	UTSW	9	105,881,778 (GRCm38)	nonsense	probably null
Z1088:Col6a5	UTSW	9	105,926,067 (GRCm38)	missense	unknown
Z1177:Col6a5	UTSW	9	105,930,785 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACGGTGAAGAGCCTTTACCTC -3'
(R):5'- AACTTGAAGTTGTGAATGCCG -3'

Sequencing Primer
(F):5'- GAAGAGCCTTTACCTCAGAGACCTTG -3'
(R):5'- ATTTTCACCGTGGCCAAAC -3'

Posted On

2019-12-04