Mutagenetix > Incidental Mutation

Incidental Mutation 'RF020:Zc3h11a'

603766

Institutional Source

Beutler Lab

Gene Symbol

Zc3h11a

Ensembl Gene

ENSMUSG00000116275

Gene Name

zinc finger CCCH type containing 11A

Synonyms

1110003F06Rik, G630041M05Rik, 5730454B08Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

RF020 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

133547600-133589137 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 133554735 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 415 (E415G)

Ref Sequence

ENSEMBL: ENSMUSP00000141255 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027736] [ENSMUST00000179598] [ENSMUST00000186476] [ENSMUST00000191896]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000027736
AA Change: E415G

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000027736
Gene: ENSMUSG00000116275
AA Change: E415G

Domain	Start	End	E-Value	Type
ZnF_C3H1	3	28	4.26e-1	SMART
ZnF_C3H1	31	56	7.62e0	SMART
ZnF_C3H1	60	86	6.83e1	SMART
low complexity region	161	176	N/A	INTRINSIC
low complexity region	218	241	N/A	INTRINSIC
low complexity region	367	376	N/A	INTRINSIC
low complexity region	625	647	N/A	INTRINSIC
low complexity region	717	730	N/A	INTRINSIC
low complexity region	770	788	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000179598

SMART Domains

Protein: ENSMUSP00000136026
Gene: ENSMUSG00000094410

Domain	Start	End	E-Value	Type
low complexity region	57	84	N/A	INTRINSIC
ZnF_BED	130	183	1.42e-8	SMART
low complexity region	203	215	N/A	INTRINSIC
ZnF_BED	265	318	5.37e-9	SMART
low complexity region	840	862	N/A	INTRINSIC
Pfam:Dimer_Tnp_hAT	869	950	9.3e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000186476

SMART Domains

Protein: ENSMUSP00000139417
Gene: ENSMUSG00000094410

Domain	Start	End	E-Value	Type
low complexity region	57	84	N/A	INTRINSIC
ZnF_BED	130	183	1.42e-8	SMART
low complexity region	203	215	N/A	INTRINSIC
ZnF_BED	265	318	5.37e-9	SMART
low complexity region	840	862	N/A	INTRINSIC
Pfam:Dimer_Tnp_hAT	869	950	1.9e-12	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000191896
AA Change: E415G

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000141255
Gene: ENSMUSG00000102976
AA Change: E415G

Domain	Start	End	E-Value	Type
ZnF_C3H1	3	28	4.26e-1	SMART
ZnF_C3H1	31	56	7.62e0	SMART
ZnF_C3H1	60	86	6.83e1	SMART
low complexity region	161	176	N/A	INTRINSIC
low complexity region	218	241	N/A	INTRINSIC
low complexity region	367	376	N/A	INTRINSIC
low complexity region	625	647	N/A	INTRINSIC
low complexity region	717	730	N/A	INTRINSIC
low complexity region	770	788	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000195669

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.3%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	A	G	7: 120,132,880 (GRCm39)	K1270E	possibly damaging	Het
Adgrb2	T	C	4: 129,903,877 (GRCm39)	S668P	probably damaging	Het
Ahdc1	T	G	4: 132,791,588 (GRCm39)	L943R	possibly damaging	Het
Ank2	T	C	3: 126,739,125 (GRCm39)	K2253R	unknown	Het
Arhgap23	T	C	11: 97,354,387 (GRCm39)	S767P	probably damaging	Het
Arhgef12	A	G	9: 42,901,285 (GRCm39)	I839T	possibly damaging	Het
Begain	CGCCGC	CGCCGCGGCCGC	12: 108,999,350 (GRCm39)		probably benign	Het
Btbd19	A	T	4: 116,979,472 (GRCm39)	C116S	probably damaging	Het
Cbr1	C	T	16: 93,407,067 (GRCm39)	A261V	probably benign	Het
Ccdc137	A	G	11: 120,349,022 (GRCm39)	R18G	probably benign	Het
Cdsn	AG	AGACAGGAAGTAGTAGCTCTCAG	17: 35,865,876 (GRCm39)		probably benign	Het
Celsr3	C	T	9: 108,726,256 (GRCm39)	R3162C	probably benign	Het
Cep350	A	T	1: 155,791,224 (GRCm39)	V1300E	probably benign	Het
Cluh	G	GCCAGAT	11: 74,560,364 (GRCm39)		probably benign	Het
Cmya5	G	T	13: 93,205,799 (GRCm39)	Q3357K	possibly damaging	Het
Col6a2	T	G	10: 76,442,043 (GRCm39)		probably null	Het
Cyp2j9	T	A	4: 96,465,889 (GRCm39)	T315S	probably damaging	Het
Cyp3a13	CATTATT	CATT	5: 137,892,525 (GRCm39)		probably null	Het
Dnah6	T	A	6: 73,095,040 (GRCm39)	Y2181F	probably benign	Het
Dnah7b	A	G	1: 46,412,421 (GRCm39)	D4010G	possibly damaging	Het
E4f1	CCG	CCGACG	17: 24,674,169 (GRCm39)		probably benign	Het
Ep300	A	T	15: 81,470,772 (GRCm39)		probably benign	Het
Fam234a	A	G	17: 26,437,725 (GRCm39)	V90A	probably benign	Het
Gab3	TCT	TCTGCT	X: 74,043,623 (GRCm39)		probably benign	Het
Gal3st1	A	G	11: 3,948,153 (GRCm39)	Y120C	possibly damaging	Het
Garin5a	GGA	GGAAGGGTGGATCCTGGATACCTGGGTCTGAGGGAAGA	7: 44,149,959 (GRCm39)		probably null	Het
Gm5475	GTGGAAGGAAAGGT	G	15: 100,325,030 (GRCm39)		probably null	Het
Gm6665	T	TC	18: 31,953,430 (GRCm39)		probably null	Het
Hdlbp	T	C	1: 93,368,456 (GRCm39)	T8A	probably benign	Het
Hnrnpa2b1	T	A	6: 51,443,674 (GRCm39)	K92N	probably damaging	Het
Hsdl2	GGAGCAGCCACAGCTGCAGGAGAAGCCACAGCTGCAGGAGCAGCCACAGC	GGAGCAGCCACAGCTGCAGGAGCAGCCACAGCTGCAGGAGAAGCCACAGCTGCAGGAGCAGCCACAGC	4: 59,610,640 (GRCm39)		probably benign	Het
Klhl10	C	G	11: 100,332,896 (GRCm39)	Q14E	probably benign	Het
Klra2	GAAAGAAATCCA	GAAAGAAATCCAAAGAAATCCA	6: 131,198,801 (GRCm39)		probably null	Het
Kmt2b	CC	CCTCCTGC	7: 30,285,807 (GRCm39)		probably benign	Het
Krt1c	A	G	15: 101,726,403 (GRCm39)	I45T	unknown	Het
Ksr2	T	C	5: 117,693,283 (GRCm39)	S244P	probably benign	Het
Lama5	A	T	2: 179,837,971 (GRCm39)	M915K	probably benign	Het
Lrp1b	A	C	2: 41,660,858 (GRCm39)	H197Q		Het
Lrrc1	T	C	9: 77,359,913 (GRCm39)	E293G	probably damaging	Het
Med12l	AACA	AACAACA	3: 59,183,379 (GRCm39)		probably benign	Het
Mgam	T	A	6: 40,662,243 (GRCm39)	Y1179N	probably damaging	Het
Mgat4c	A	G	10: 102,224,928 (GRCm39)	I381V	probably benign	Het
Mrgprx1	A	AGAC	7: 47,671,259 (GRCm39)		probably benign	Het
Myc	A	T	15: 61,857,672 (GRCm39)		probably benign	Het
Nipbl	A	G	15: 8,388,418 (GRCm39)	S401P	probably damaging	Het
Nlrp9c	A	T	7: 26,084,649 (GRCm39)	I310N	probably benign	Het
Nwd2	C	A	5: 63,963,066 (GRCm39)	Y883*	probably null	Het
Oas1e	T	A	5: 120,932,383 (GRCm39)	T87S	possibly damaging	Het
Or10n7-ps1	GA	GATACA	9: 39,598,049 (GRCm39)		probably null	Het
Or51a5	A	T	7: 102,771,098 (GRCm39)	C294S	probably benign	Het
Or52d3	A	G	7: 104,229,497 (GRCm39)	M215V	probably benign	Het
Or8b38	G	A	9: 37,972,620 (GRCm39)	M1I	probably null	Het
Pappa	T	G	4: 65,123,282 (GRCm39)	S872R	possibly damaging	Het
Parp4	T	C	14: 56,884,806 (GRCm39)	L1295P	unknown	Het
Pcdh15	A	T	10: 74,021,242 (GRCm39)	Y152F	probably damaging	Het
Pdk1	A	T	2: 71,714,240 (GRCm39)	I217L	possibly damaging	Het
Phldb1	A	C	9: 44,609,243 (GRCm39)	C450W	probably damaging	Het
Pnma8a	C	CCATGATGCACCTGCTTCAACATCA	7: 16,695,376 (GRCm39)		probably benign	Het
Prl2c5	G	A	13: 13,360,497 (GRCm39)	G55S	probably benign	Het
Psme2b	A	T	11: 48,836,397 (GRCm39)	H183Q	probably damaging	Het
Ptms	CCTCCTC	CCTCCTCCTC	6: 124,891,412 (GRCm39)		probably benign	Het
Rln3	T	C	8: 84,769,931 (GRCm39)	T73A	probably benign	Het
Rprd1a	T	A	18: 24,663,062 (GRCm39)	Q21L	probably damaging	Het
Septin3	A	T	15: 82,168,662 (GRCm39)	D155V	probably damaging	Het
Sh3rf3	T	A	10: 58,649,590 (GRCm39)	V65E	probably damaging	Het
Shank3	A	G	15: 89,384,593 (GRCm39)	N155S	probably benign	Het
Shox2	G	T	3: 66,881,146 (GRCm39)	P278Q	probably damaging	Het
Slc1a1	T	C	19: 28,856,555 (GRCm39)		probably null	Het
Slc39a10	A	T	1: 46,849,175 (GRCm39)	F814I	probably damaging	Het
Slc5a1	T	C	5: 33,290,773 (GRCm39)	I119T	probably damaging	Het
Slc6a13	T	C	6: 121,301,310 (GRCm39)		probably null	Het
Spmap2l	CAGCGATCCTCCCCAGTCCCGCAAGGC	CAGCGATCCTCCCCAGTCCCGCAAGGCGAGCGATCCTCCCCAGTCCCGCAAGGC	5: 77,164,247 (GRCm39)		probably benign	Het
Spta1	A	G	1: 174,041,010 (GRCm39)	D1270G	probably benign	Het
Spta1	T	A	1: 174,045,469 (GRCm39)	F1542L	probably damaging	Het
Tacc3	T	A	5: 33,818,568 (GRCm39)	M1K	probably null	Het
Tax1bp1	T	C	6: 52,698,339 (GRCm39)	V17A	probably damaging	Het
Tmem156	T	A	5: 65,248,890 (GRCm39)	E3D	probably benign	Het
Tmem209	T	A	6: 30,487,417 (GRCm39)	M530L	probably benign	Het
Trpc2	A	G	7: 101,745,433 (GRCm39)	D883G	unknown	Het
Tsen34	GGAGCCAAAAT	G	7: 3,698,795 (GRCm39)		probably null	Het
Uhrf2	T	C	19: 30,063,791 (GRCm39)	Y585H	probably damaging	Het
Vmn1r26	T	A	6: 57,985,705 (GRCm39)	K161N	probably benign	Het
Vmn1r29	A	G	6: 58,284,528 (GRCm39)	S83G	probably benign	Het
Vps13b	G	T	15: 35,925,552 (GRCm39)	W3829L	probably null	Het
Vwce	G	A	19: 10,630,449 (GRCm39)	G503R	probably damaging	Het
Zc3h14	T	A	12: 98,746,541 (GRCm39)		probably null	Het
Zfp119b	A	T	17: 56,246,499 (GRCm39)	M229K	probably benign	Het
Zfp384	CCAAGCTCAAGC	CCAAGC	6: 125,013,418 (GRCm39)		probably benign	Het
Zfp384	GGCCCAGGC	GGCCCAGGCCCACGCCCAGGC	6: 125,013,451 (GRCm39)		probably benign	Het
Zyx	T	A	6: 42,334,330 (GRCm39)	L518Q	probably damaging	Het

Other mutations in Zc3h11a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01329:Zc3h11a	APN	1	133,553,600 (GRCm39)	missense	probably benign
IGL01961:Zc3h11a	APN	1	133,554,805 (GRCm39)	missense	probably benign	0.12
IGL02005:Zc3h11a	APN	1	133,549,880 (GRCm39)	missense	probably benign
IGL02365:Zc3h11a	APN	1	133,565,151 (GRCm39)	missense	probably benign	0.02
IGL02454:Zc3h11a	APN	1	133,552,254 (GRCm39)	missense	probably benign	0.09
PIT4449001:Zc3h11a	UTSW	1	133,552,349 (GRCm39)	missense	probably benign	0.22
R0180:Zc3h11a	UTSW	1	133,549,349 (GRCm39)	missense	probably benign	0.11
R0965:Zc3h11a	UTSW	1	133,573,541 (GRCm39)	missense	possibly damaging	0.80
R1389:Zc3h11a	UTSW	1	133,561,541 (GRCm39)	missense	probably damaging	0.99
R1607:Zc3h11a	UTSW	1	133,552,425 (GRCm39)	missense	probably benign
R1639:Zc3h11a	UTSW	1	133,552,446 (GRCm39)	missense	probably benign	0.03
R1720:Zc3h11a	UTSW	1	133,549,439 (GRCm39)	missense	probably damaging	0.97
R1728:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R1728:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1729:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1730:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R1730:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1739:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R1739:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1762:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R1762:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1783:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R1783:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1784:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R1784:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1785:Zc3h11a	UTSW	1	133,549,892 (GRCm39)	missense	probably benign
R1785:Zc3h11a	UTSW	1	133,552,359 (GRCm39)	missense	probably benign	0.20
R2508:Zc3h11a	UTSW	1	133,552,521 (GRCm39)	missense	probably benign	0.01
R4792:Zc3h11a	UTSW	1	133,568,436 (GRCm39)	missense	probably damaging	0.98
R4901:Zc3h11a	UTSW	1	133,552,449 (GRCm39)	missense	probably benign	0.00
R4932:Zc3h11a	UTSW	1	133,552,350 (GRCm39)	missense	probably benign	0.00
R5135:Zc3h11a	UTSW	1	133,561,527 (GRCm39)	missense	probably benign	0.00
R5186:Zc3h11a	UTSW	1	133,549,412 (GRCm39)	missense	probably damaging	0.99
R5357:Zc3h11a	UTSW	1	133,550,780 (GRCm39)	missense	probably damaging	1.00
R5438:Zc3h11a	UTSW	1	133,568,385 (GRCm39)	missense	probably damaging	1.00
R6149:Zc3h11a	UTSW	1	133,566,613 (GRCm39)	nonsense	probably null
R6268:Zc3h11a	UTSW	1	133,552,295 (GRCm39)	missense	probably benign	0.01
R6385:Zc3h11a	UTSW	1	133,565,192 (GRCm39)	missense	possibly damaging	0.82
R6847:Zc3h11a	UTSW	1	133,566,700 (GRCm39)	splice site	probably null
R7107:Zc3h11a	UTSW	1	133,566,655 (GRCm39)	missense	probably damaging	0.96
R7543:Zc3h11a	UTSW	1	133,554,768 (GRCm39)	missense	possibly damaging	0.49
R7693:Zc3h11a	UTSW	1	133,573,475 (GRCm39)	missense	probably damaging	1.00
R7742:Zc3h11a	UTSW	1	133,565,173 (GRCm39)	missense	probably benign	0.01
R8485:Zc3h11a	UTSW	1	133,553,633 (GRCm39)	missense	possibly damaging	0.75
R8911:Zc3h11a	UTSW	1	133,566,339 (GRCm39)	missense	probably damaging	0.99
R9539:Zc3h11a	UTSW	1	133,554,927 (GRCm39)	missense	probably benign	0.06
RF018:Zc3h11a	UTSW	1	133,554,853 (GRCm39)	missense	possibly damaging	0.66

Predicted Primers

PCR Primer
(F):5'- AACCTGCTCTCTTGGTGATGC -3'
(R):5'- AAGAACCTTCAGGAGCCGATG -3'

Sequencing Primer
(F):5'- CGGAGCAGCCTCTTTGTC -3'
(R):5'- AGGAGCCGATGATTCTCCATCAG -3'

Posted On

2019-12-04