Mutagenetix > Incidental Mutation

Incidental Mutation 'RF020:Lama5'

603772

Institutional Source

Beutler Lab

Gene Symbol

Lama5

Ensembl Gene

ENSMUSG00000015647

Gene Name

laminin, alpha 5

Synonyms

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

RF020 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

180176373-180225859 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 180196178 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 915 (M915K)

Ref Sequence

ENSEMBL: ENSMUSP00000015791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015791]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000015791
AA Change: M915K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647
AA Change: M915K

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.3%
20x: 98.5%

Validation Efficiency

MGI Phenotype

Strain: 3624772; 1934917
Lethality: E1-E17
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit disrupted basal laminae leading to exencephaly, syndactyly, placentopathy, kidney defects, abnormal lobar septation with absence of a visceral pleural membrane, and lethality in late gestation. [provided by MGI curators]

Allele List at MGI

All alleles(49) : Targeted(5) Gene trapped(44)

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	A	G	7: 120,533,657 (GRCm38)	K1270E	possibly damaging	Het
Adgrb2	T	C	4: 130,010,084 (GRCm38)	S668P	probably damaging	Het
Ahdc1	T	G	4: 133,064,277 (GRCm38)	L943R	possibly damaging	Het
Ank2	T	C	3: 126,945,476 (GRCm38)	K2253R	unknown	Het
Arhgap23	T	C	11: 97,463,561 (GRCm38)	S767P	probably damaging	Het
Arhgef12	A	G	9: 42,989,989 (GRCm38)	I839T	possibly damaging	Het
Begain	CGCCGC	CGCCGCGGCCGC	12: 109,033,424 (GRCm38)		probably benign	Het
Btbd19	A	T	4: 117,122,275 (GRCm38)	C116S	probably damaging	Het
Cbr1	C	T	16: 93,610,179 (GRCm38)	A261V	probably benign	Het
Ccdc137	A	G	11: 120,458,196 (GRCm38)	R18G	probably benign	Het
Cdsn	AG	AGACAGGAAGTAGTAGCTCTCAG	17: 35,554,979 (GRCm38)		probably benign	Het
Celsr3	C	T	9: 108,849,057 (GRCm38)	R3162C	probably benign	Het
Cep350	A	T	1: 155,915,478 (GRCm38)	V1300E	probably benign	Het
Cluh	G	GCCAGAT	11: 74,669,538 (GRCm38)		probably benign	Het
Cmya5	G	T	13: 93,069,291 (GRCm38)	Q3357K	possibly damaging	Het
Col6a2	T	G	10: 76,606,209 (GRCm38)		probably null	Het
Cyp2j9	T	A	4: 96,577,652 (GRCm38)	T315S	probably damaging	Het
Cyp3a13	CATTATT	CATT	5: 137,894,263 (GRCm38)		probably null	Het
Dnah6	T	A	6: 73,118,057 (GRCm38)	Y2181F	probably benign	Het
Dnah7b	A	G	1: 46,373,261 (GRCm38)	D4010G	possibly damaging	Het
E4f1	CCG	CCGACG	17: 24,455,195 (GRCm38)		probably benign	Het
Ep300	A	T	15: 81,586,571 (GRCm38)		probably benign	Het
Fam234a	A	G	17: 26,218,751 (GRCm38)	V90A	probably benign	Het
Gab3	TCT	TCTGCT	X: 75,000,017 (GRCm38)		probably benign	Het
Gal3st1	A	G	11: 3,998,153 (GRCm38)	Y120C	possibly damaging	Het
Garin5a	GGA	GGAAGGGTGGATCCTGGATACCTGGGTCTGAGGGAAGA	7: 44,500,535 (GRCm38)		probably null	Het
Gm5475	GTGGAAGGAAAGGT	G	15: 100,427,149 (GRCm38)		probably null	Het
Gm6665	T	TC	18: 31,820,377 (GRCm38)		probably null	Het
Hdlbp	T	C	1: 93,440,734 (GRCm38)	T8A	probably benign	Het
Hnrnpa2b1	T	A	6: 51,466,694 (GRCm38)	K92N	probably damaging	Het
Hsdl2	GGAGCAGCCACAGCTGCAGGAGAAGCCACAGCTGCAGGAGCAGCCACAGC	GGAGCAGCCACAGCTGCAGGAGCAGCCACAGCTGCAGGAGAAGCCACAGCTGCAGGAGCAGCCACAGC	4: 59,610,640 (GRCm38)		probably benign	Het
Klhl10	C	G	11: 100,442,070 (GRCm38)	Q14E	probably benign	Het
Klra2	GAAAGAAATCCA	GAAAGAAATCCAAAGAAATCCA	6: 131,221,838 (GRCm38)		probably null	Het
Kmt2b	CC	CCTCCTGC	7: 30,586,382 (GRCm38)		probably benign	Het
Krt2	A	G	15: 101,817,968 (GRCm38)	I45T	unknown	Het
Ksr2	T	C	5: 117,555,218 (GRCm38)	S244P	probably benign	Het
Lrp1b	A	C	2: 41,770,846 (GRCm38)	H197Q		Het
Lrrc1	T	C	9: 77,452,631 (GRCm38)	E293G	probably damaging	Het
Med12l	AACA	AACAACA	3: 59,275,958 (GRCm38)		probably benign	Het
Mgam	T	A	6: 40,685,309 (GRCm38)	Y1179N	probably damaging	Het
Mgat4c	A	G	10: 102,389,067 (GRCm38)	I381V	probably benign	Het
Mrgprx1	A	AGAC	7: 48,021,511 (GRCm38)		probably benign	Het
Myc	A	T	15: 61,985,823 (GRCm38)		probably benign	Het
Nipbl	A	G	15: 8,358,934 (GRCm38)	S401P	probably damaging	Het
Nlrp9c	A	T	7: 26,385,224 (GRCm38)	I310N	probably benign	Het
Nwd2	C	A	5: 63,805,723 (GRCm38)	Y883*	probably null	Het
Oas1e	T	A	5: 120,794,318 (GRCm38)	T87S	possibly damaging	Het
Or10n7-ps1	GA	GATACA	9: 39,686,753 (GRCm38)		probably null	Het
Or51a5	A	T	7: 103,121,891 (GRCm38)	C294S	probably benign	Het
Or52d3	A	G	7: 104,580,290 (GRCm38)	M215V	probably benign	Het
Or8b38	G	A	9: 38,061,324 (GRCm38)	M1I	probably null	Het
Pappa	T	G	4: 65,205,045 (GRCm38)	S872R	possibly damaging	Het
Parp4	T	C	14: 56,647,349 (GRCm38)	L1295P	unknown	Het
Pcdh15	A	T	10: 74,185,410 (GRCm38)	Y152F	probably damaging	Het
Pdk1	A	T	2: 71,883,896 (GRCm38)	I217L	possibly damaging	Het
Phldb1	A	C	9: 44,697,946 (GRCm38)	C450W	probably damaging	Het
Pnma8a	C	CCATGATGCACCTGCTTCAACATCA	7: 16,961,451 (GRCm38)		probably benign	Het
Prl2c5	G	A	13: 13,185,912 (GRCm38)	G55S	probably benign	Het
Psme2b	A	T	11: 48,945,570 (GRCm38)	H183Q	probably damaging	Het
Ptms	CCTCCTC	CCTCCTCCTC	6: 124,914,449 (GRCm38)		probably benign	Het
Rln3	T	C	8: 84,043,302 (GRCm38)	T73A	probably benign	Het
Rprd1a	T	A	18: 24,530,005 (GRCm38)	Q21L	probably damaging	Het
Septin3	A	T	15: 82,284,461 (GRCm38)	D155V	probably damaging	Het
Sh3rf3	T	A	10: 58,813,768 (GRCm38)	V65E	probably damaging	Het
Shank3	A	G	15: 89,500,390 (GRCm38)	N155S	probably benign	Het
Shox2	G	T	3: 66,973,813 (GRCm38)	P278Q	probably damaging	Het
Slc1a1	T	C	19: 28,879,155 (GRCm38)		probably null	Het
Slc39a10	A	T	1: 46,810,015 (GRCm38)	F814I	probably damaging	Het
Slc5a1	T	C	5: 33,133,429 (GRCm38)	I119T	probably damaging	Het
Slc6a13	T	C	6: 121,324,351 (GRCm38)		probably null	Het
Spmap2l	CAGCGATCCTCCCCAGTCCCGCAAGGC	CAGCGATCCTCCCCAGTCCCGCAAGGCGAGCGATCCTCCCCAGTCCCGCAAGGC	5: 77,016,400 (GRCm38)		probably benign	Het
Spta1	T	A	1: 174,217,903 (GRCm38)	F1542L	probably damaging	Het
Spta1	A	G	1: 174,213,444 (GRCm38)	D1270G	probably benign	Het
Tacc3	T	A	5: 33,661,224 (GRCm38)	M1K	probably null	Het
Tax1bp1	T	C	6: 52,721,354 (GRCm38)	V17A	probably damaging	Het
Tmem156	T	A	5: 65,091,547 (GRCm38)	E3D	probably benign	Het
Tmem209	T	A	6: 30,487,418 (GRCm38)	M530L	probably benign	Het
Trpc2	A	G	7: 102,096,226 (GRCm38)	D883G	unknown	Het
Tsen34	GGAGCCAAAAT	G	7: 3,695,796 (GRCm38)		probably null	Het
Uhrf2	T	C	19: 30,086,391 (GRCm38)	Y585H	probably damaging	Het
Vmn1r26	T	A	6: 58,008,720 (GRCm38)	K161N	probably benign	Het
Vmn1r29	A	G	6: 58,307,543 (GRCm38)	S83G	probably benign	Het
Vps13b	G	T	15: 35,925,406 (GRCm38)	W3829L	probably null	Het
Vwce	G	A	19: 10,653,085 (GRCm38)	G503R	probably damaging	Het
Zc3h11a	T	C	1: 133,626,997 (GRCm38)	E415G	possibly damaging	Het
Zc3h14	T	A	12: 98,780,282 (GRCm38)		probably null	Het
Zfp119b	A	T	17: 55,939,499 (GRCm38)	M229K	probably benign	Het
Zfp384	GGCCCAGGC	GGCCCAGGCCCACGCCCAGGC	6: 125,036,488 (GRCm38)		probably benign	Het
Zfp384	CCAAGCTCAAGC	CCAAGC	6: 125,036,455 (GRCm38)		probably benign	Het
Zyx	T	A	6: 42,357,396 (GRCm38)	L518Q	probably damaging	Het

Other mutations in Lama5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Lama5	APN	2	180,176,543 (GRCm38)	unclassified	probably benign
IGL01370:Lama5	APN	2	180,197,400 (GRCm38)	missense	possibly damaging	0.87
IGL01474:Lama5	APN	2	180,196,570 (GRCm38)	missense	probably damaging	1.00
IGL01614:Lama5	APN	2	180,180,864 (GRCm38)	missense	probably damaging	1.00
IGL01941:Lama5	APN	2	180,192,392 (GRCm38)	missense	possibly damaging	0.71
IGL01953:Lama5	APN	2	180,190,704 (GRCm38)	missense	probably damaging	0.97
IGL02093:Lama5	APN	2	180,188,587 (GRCm38)	missense	probably damaging	1.00
IGL02197:Lama5	APN	2	180,207,219 (GRCm38)	missense	possibly damaging	0.82
IGL02308:Lama5	APN	2	180,190,327 (GRCm38)	splice site	probably benign
IGL02314:Lama5	APN	2	180,194,482 (GRCm38)	splice site	probably benign
IGL02317:Lama5	APN	2	180,191,319 (GRCm38)	missense	probably damaging	1.00
IGL02354:Lama5	APN	2	180,193,884 (GRCm38)	nonsense	probably null
IGL02361:Lama5	APN	2	180,193,884 (GRCm38)	nonsense	probably null
IGL02557:Lama5	APN	2	180,190,932 (GRCm38)	nonsense	probably null
IGL03026:Lama5	APN	2	180,195,967 (GRCm38)	missense	probably benign	0.34
IGL03160:Lama5	APN	2	180,180,335 (GRCm38)	missense	probably damaging	1.00
IGL03238:Lama5	APN	2	180,188,574 (GRCm38)	missense	probably benign
IGL03390:Lama5	APN	2	180,207,218 (GRCm38)	missense	probably damaging	1.00
blancmange	UTSW	2	180,180,611 (GRCm38)	missense	probably damaging	0.98
cupcake	UTSW	2	180,185,959 (GRCm38)	missense	probably damaging	1.00
layercake	UTSW	2	180,180,718 (GRCm38)	missense	possibly damaging	0.83
poundcake	UTSW	2	180,195,608 (GRCm38)	missense	probably damaging	1.00
Salty	UTSW	2	180,181,651 (GRCm38)	missense	possibly damaging	0.84
PIT4378001:Lama5	UTSW	2	180,189,445 (GRCm38)	missense	possibly damaging	0.89
R0003:Lama5	UTSW	2	180,178,079 (GRCm38)	splice site	probably null
R0056:Lama5	UTSW	2	180,187,106 (GRCm38)	intron	probably benign
R0147:Lama5	UTSW	2	180,190,406 (GRCm38)	missense	probably benign
R0148:Lama5	UTSW	2	180,190,406 (GRCm38)	missense	probably benign
R0310:Lama5	UTSW	2	180,181,566 (GRCm38)	splice site	probably benign
R0326:Lama5	UTSW	2	180,182,426 (GRCm38)	missense	possibly damaging	0.90
R0368:Lama5	UTSW	2	180,181,230 (GRCm38)	nonsense	probably null
R0479:Lama5	UTSW	2	180,184,457 (GRCm38)	missense	probably benign	0.03
R0490:Lama5	UTSW	2	180,180,169 (GRCm38)	missense	possibly damaging	0.90
R0636:Lama5	UTSW	2	180,189,331 (GRCm38)	critical splice donor site	probably null
R0704:Lama5	UTSW	2	180,179,484 (GRCm38)	missense	possibly damaging	0.84
R0733:Lama5	UTSW	2	180,180,718 (GRCm38)	missense	possibly damaging	0.83
R1017:Lama5	UTSW	2	180,195,420 (GRCm38)	missense	probably damaging	1.00
R1078:Lama5	UTSW	2	180,179,764 (GRCm38)	unclassified	probably benign
R1294:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1423:Lama5	UTSW	2	180,195,641 (GRCm38)	missense	probably damaging	1.00
R1438:Lama5	UTSW	2	180,182,800 (GRCm38)	missense	probably benign	0.01
R1447:Lama5	UTSW	2	180,185,878 (GRCm38)	missense	probably damaging	0.99
R1540:Lama5	UTSW	2	180,180,151 (GRCm38)	missense	probably benign
R1601:Lama5	UTSW	2	180,197,745 (GRCm38)	missense	probably damaging	1.00
R1624:Lama5	UTSW	2	180,206,758 (GRCm38)	missense	probably benign	0.02
R1674:Lama5	UTSW	2	180,201,987 (GRCm38)	missense	probably benign	0.00
R1687:Lama5	UTSW	2	180,194,066 (GRCm38)	missense	probably benign	0.00
R1696:Lama5	UTSW	2	180,202,486 (GRCm38)	missense	probably damaging	1.00
R1701:Lama5	UTSW	2	180,221,369 (GRCm38)	missense	probably damaging	1.00
R1778:Lama5	UTSW	2	180,195,481 (GRCm38)	splice site	probably benign
R1936:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1939:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1940:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1953:Lama5	UTSW	2	180,190,747 (GRCm38)	missense	possibly damaging	0.94
R1966:Lama5	UTSW	2	180,188,352 (GRCm38)	missense	probably damaging	1.00
R2024:Lama5	UTSW	2	180,179,130 (GRCm38)	missense	probably benign	0.00
R2079:Lama5	UTSW	2	180,225,508 (GRCm38)	missense	possibly damaging	0.68
R2115:Lama5	UTSW	2	180,186,885 (GRCm38)	missense	probably damaging	1.00
R2173:Lama5	UTSW	2	180,196,242 (GRCm38)	missense	probably benign	0.00
R2272:Lama5	UTSW	2	180,178,603 (GRCm38)	missense	possibly damaging	0.93
R2357:Lama5	UTSW	2	180,180,097 (GRCm38)	missense	probably benign	0.01
R2860:Lama5	UTSW	2	180,187,247 (GRCm38)	missense	probably benign	0.00
R2861:Lama5	UTSW	2	180,187,247 (GRCm38)	missense	probably benign	0.00
R2939:Lama5	UTSW	2	180,198,954 (GRCm38)	missense	probably damaging	1.00
R3053:Lama5	UTSW	2	180,183,067 (GRCm38)	missense	probably damaging	0.99
R3430:Lama5	UTSW	2	180,196,317 (GRCm38)	missense	probably benign	0.00
R3752:Lama5	UTSW	2	180,187,222 (GRCm38)	missense	probably damaging	1.00
R3782:Lama5	UTSW	2	180,194,563 (GRCm38)	missense	possibly damaging	0.57
R3901:Lama5	UTSW	2	180,182,351 (GRCm38)	splice site	probably benign
R4248:Lama5	UTSW	2	180,180,427 (GRCm38)	missense	possibly damaging	0.84
R4626:Lama5	UTSW	2	180,184,460 (GRCm38)	missense	probably damaging	0.98
R4638:Lama5	UTSW	2	180,190,413 (GRCm38)	missense	possibly damaging	0.89
R4669:Lama5	UTSW	2	180,180,637 (GRCm38)	missense	probably damaging	1.00
R4673:Lama5	UTSW	2	180,199,266 (GRCm38)	missense	probably damaging	1.00
R4677:Lama5	UTSW	2	180,179,366 (GRCm38)	missense	possibly damaging	0.69
R4701:Lama5	UTSW	2	180,191,696 (GRCm38)	missense	probably damaging	1.00
R4774:Lama5	UTSW	2	180,185,941 (GRCm38)	missense	probably damaging	1.00
R4880:Lama5	UTSW	2	180,177,068 (GRCm38)	unclassified	probably benign
R4923:Lama5	UTSW	2	180,184,149 (GRCm38)	missense	probably benign	0.18
R4960:Lama5	UTSW	2	180,208,252 (GRCm38)	critical splice donor site	probably null
R4983:Lama5	UTSW	2	180,193,449 (GRCm38)	missense	probably benign	0.13
R5061:Lama5	UTSW	2	180,198,786 (GRCm38)	nonsense	probably null
R5080:Lama5	UTSW	2	180,207,200 (GRCm38)	nonsense	probably null
R5135:Lama5	UTSW	2	180,202,220 (GRCm38)	missense	possibly damaging	0.89
R5206:Lama5	UTSW	2	180,191,304 (GRCm38)	missense	probably damaging	1.00
R5296:Lama5	UTSW	2	180,193,801 (GRCm38)	missense	probably damaging	1.00
R5319:Lama5	UTSW	2	180,181,118 (GRCm38)	missense	probably damaging	1.00
R5355:Lama5	UTSW	2	180,181,651 (GRCm38)	missense	possibly damaging	0.84
R5388:Lama5	UTSW	2	180,190,746 (GRCm38)	missense	possibly damaging	0.83
R5528:Lama5	UTSW	2	180,194,563 (GRCm38)	missense	probably benign	0.21
R5536:Lama5	UTSW	2	180,189,349 (GRCm38)	missense	probably damaging	0.99
R5658:Lama5	UTSW	2	180,208,276 (GRCm38)	nonsense	probably null
R5823:Lama5	UTSW	2	180,192,492 (GRCm38)	missense	probably benign	0.04
R5885:Lama5	UTSW	2	180,201,831 (GRCm38)	missense	probably damaging	1.00
R5889:Lama5	UTSW	2	180,193,674 (GRCm38)	intron	probably benign
R5912:Lama5	UTSW	2	180,195,475 (GRCm38)	missense	probably damaging	1.00
R5955:Lama5	UTSW	2	180,197,474 (GRCm38)	missense	probably damaging	1.00
R6015:Lama5	UTSW	2	180,185,392 (GRCm38)	missense	probably benign	0.36
R6037:Lama5	UTSW	2	180,207,013 (GRCm38)	missense	probably damaging	1.00
R6037:Lama5	UTSW	2	180,207,013 (GRCm38)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	180,185,959 (GRCm38)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	180,180,611 (GRCm38)	missense	probably damaging	0.98
R6359:Lama5	UTSW	2	180,195,982 (GRCm38)	missense	probably benign	0.01
R6385:Lama5	UTSW	2	180,196,533 (GRCm38)	missense	probably damaging	1.00
R6406:Lama5	UTSW	2	180,197,464 (GRCm38)	nonsense	probably null
R6552:Lama5	UTSW	2	180,181,154 (GRCm38)	missense	probably damaging	0.98
R6632:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6633:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6645:Lama5	UTSW	2	180,179,670 (GRCm38)	missense	probably damaging	1.00
R6731:Lama5	UTSW	2	180,188,574 (GRCm38)	missense	probably benign	0.09
R6744:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6798:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6799:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6801:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6851:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6869:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6881:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6882:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6884:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R7022:Lama5	UTSW	2	180,180,731 (GRCm38)	missense	probably damaging	1.00
R7204:Lama5	UTSW	2	180,202,177 (GRCm38)	missense	probably damaging	1.00
R7207:Lama5	UTSW	2	180,207,084 (GRCm38)	missense	probably damaging	0.98
R7282:Lama5	UTSW	2	180,201,795 (GRCm38)	missense	probably damaging	1.00
R7367:Lama5	UTSW	2	180,192,958 (GRCm38)	missense	probably benign	0.01
R7410:Lama5	UTSW	2	180,202,390 (GRCm38)	critical splice donor site	probably null
R7699:Lama5	UTSW	2	180,180,861 (GRCm38)	missense	probably damaging	1.00
R7849:Lama5	UTSW	2	180,201,812 (GRCm38)	missense	probably damaging	1.00
R7909:Lama5	UTSW	2	180,192,276 (GRCm38)	missense	possibly damaging	0.95
R7948:Lama5	UTSW	2	180,202,201 (GRCm38)	missense	probably damaging	1.00
R8153:Lama5	UTSW	2	180,187,931 (GRCm38)	missense	probably benign	0.37
R8317:Lama5	UTSW	2	180,206,991 (GRCm38)	missense	probably damaging	1.00
R8351:Lama5	UTSW	2	180,195,608 (GRCm38)	missense	probably damaging	1.00
R8370:Lama5	UTSW	2	180,201,487 (GRCm38)	missense	possibly damaging	0.80
R8398:Lama5	UTSW	2	180,197,034 (GRCm38)	critical splice donor site	probably null
R8401:Lama5	UTSW	2	180,198,787 (GRCm38)	missense	probably damaging	1.00
R8404:Lama5	UTSW	2	180,195,222 (GRCm38)	missense	probably damaging	1.00
R8502:Lama5	UTSW	2	180,195,222 (GRCm38)	missense	probably damaging	1.00
R8694:Lama5	UTSW	2	180,180,884 (GRCm38)	missense	probably damaging	0.98
R8705:Lama5	UTSW	2	180,178,561 (GRCm38)	missense	probably damaging	1.00
R8732:Lama5	UTSW	2	180,186,688 (GRCm38)	missense	probably damaging	1.00
R8755:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R8786:Lama5	UTSW	2	180,196,307 (GRCm38)	missense	probably damaging	1.00
R8926:Lama5	UTSW	2	180,193,990 (GRCm38)	missense	probably benign	0.08
R8928:Lama5	UTSW	2	180,202,039 (GRCm38)	missense	probably damaging	1.00
R8953:Lama5	UTSW	2	180,193,520 (GRCm38)	missense	probably damaging	0.99
R8958:Lama5	UTSW	2	180,193,799 (GRCm38)	missense	probably benign
R9002:Lama5	UTSW	2	180,196,518 (GRCm38)	missense	probably damaging	1.00
R9081:Lama5	UTSW	2	180,192,137 (GRCm38)	nonsense	probably null
R9165:Lama5	UTSW	2	180,179,493 (GRCm38)	missense	probably damaging	0.99
R9233:Lama5	UTSW	2	180,198,709 (GRCm38)	nonsense	probably null
R9264:Lama5	UTSW	2	180,196,478 (GRCm38)	splice site	probably benign
R9311:Lama5	UTSW	2	180,196,482 (GRCm38)	critical splice donor site	probably null
R9443:Lama5	UTSW	2	180,201,729 (GRCm38)	missense	probably benign	0.00
R9488:Lama5	UTSW	2	180,181,441 (GRCm38)	missense	possibly damaging	0.95
R9674:Lama5	UTSW	2	180,198,474 (GRCm38)	critical splice donor site	probably null
R9684:Lama5	UTSW	2	180,207,245 (GRCm38)	missense	probably damaging	1.00
R9749:Lama5	UTSW	2	180,183,640 (GRCm38)	missense	probably benign	0.00
X0065:Lama5	UTSW	2	180,181,731 (GRCm38)	missense	probably benign	0.26
Z1177:Lama5	UTSW	2	180,190,714 (GRCm38)	missense	possibly damaging	0.95
Z1177:Lama5	UTSW	2	180,189,419 (GRCm38)	missense	probably damaging	1.00
Z1177:Lama5	UTSW	2	180,183,630 (GRCm38)	missense	probably benign	0.03
Z1177:Lama5	UTSW	2	180,198,810 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATCCACGGTTGACATATCGG -3'
(R):5'- CACTCAGATACCTGTTTCATGGC -3'

Sequencing Primer
(F):5'- CGGTTGACATATCGGAAAACC -3'
(R):5'- CCTGTTTCATGGCAGGCC -3'

Posted On

2019-12-04