Mutagenetix > Incidental Mutation

Incidental Mutation 'RF032:Med12l'

604399

Institutional Source

Beutler Lab

Gene Symbol

Med12l

Ensembl Gene

ENSMUSG00000056476

Gene Name

mediator complex subunit 12-like

Synonyms

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.308) question?

Stock #

RF032 (G1)

Quality Score

174.468

Status

Not validated

Chromosome

Chromosomal Location

59005825-59318682 bp(+) (GRCm38)

Type of Mutation

small insertion (1 aa in frame mutation)

DNA Base Change (assembly)

CAG to CAGAAG at 59275981 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000142903 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040325] [ENSMUST00000164225] [ENSMUST00000199659]

AlphaFold

Q8BQM9

Predicted Effect

probably benign
Transcript: ENSMUST00000040325

SMART Domains

Protein: ENSMUSP00000042269
Gene: ENSMUSG00000056476

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	730	2.6e-207	PFAM
low complexity region	744	758	N/A	INTRINSIC
low complexity region	853	872	N/A	INTRINSIC
low complexity region	1455	1466	N/A	INTRINSIC
low complexity region	1728	1742	N/A	INTRINSIC
low complexity region	1769	1783	N/A	INTRINSIC
Pfam:Med12-PQL	1803	2029	2.3e-14	PFAM
low complexity region	2055	2076	N/A	INTRINSIC
low complexity region	2083	2101	N/A	INTRINSIC
low complexity region	2116	2136	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000164225

SMART Domains

Protein: ENSMUSP00000127038
Gene: ENSMUSG00000056476

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	283	765	5e-187	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1763	1777	N/A	INTRINSIC
low complexity region	1804	1818	N/A	INTRINSIC
Pfam:Med12-PQL	1840	2063	9.7e-66	PFAM
low complexity region	2090	2111	N/A	INTRINSIC
low complexity region	2118	2136	N/A	INTRINSIC
low complexity region	2151	2171	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000197374

Predicted Effect

probably benign
Transcript: ENSMUST00000199659

SMART Domains

Protein: ENSMUSP00000142903
Gene: ENSMUSG00000056476

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	765	5.5e-209	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1761	1775	N/A	INTRINSIC
low complexity region	1802	1816	N/A	INTRINSIC
Pfam:Med12-PQL	1836	2062	1.7e-15	PFAM
low complexity region	2088	2130	N/A	INTRINSIC
low complexity region	2144	2164	N/A	INTRINSIC

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.3%
20x: 98.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010]

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	TACCT	TACCTGACCT	17: 24,287,727 (GRCm38)		probably null	Het
Acap3	CTGCTG	CTGCTGCATCCTGGGATGCTG	4: 155,905,102 (GRCm38)		probably benign	Het
Arid1b	GCG	GCGTCG	17: 4,995,588 (GRCm38)		probably benign	Het
Blm	CTCC	CTCCTCCTCCTCGTCC	7: 80,512,930 (GRCm38)		probably benign	Het
Cacna1f	GAG	GAGTAG	X: 7,620,063 (GRCm38)		probably null	Het
Calhm1	C	CTGTGGCCGTGG	19: 47,141,283 (GRCm38)		probably null	Het
Cluh	CCCGAGCC	CCCGAGCCCGAGCC	11: 74,669,515 (GRCm38)		probably benign	Het
Cyb5r4	ACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	ACTGCCCAGGGATGTGACAGACACGCTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	9: 87,040,413 (GRCm38)		probably benign	Het
Dmkn	GT	GTTGTGAAAGTGGTGGAAGTGGTGGAATT	7: 30,767,182 (GRCm38)		probably benign	Het
Efhd2	CGCC	CGCCGCAGCC	4: 141,874,772 (GRCm38)		probably benign	Het
Enah	TGGCGGTGG	TG	1: 181,921,929 (GRCm38)		probably null	Het
Gm8369	GTGTGT	GTGTGTATGTGT	19: 11,511,778 (GRCm38)		probably benign	Het
H2-T10	TTTCCCACTGTA	T	17: 36,120,294 (GRCm38)		probably null	Het
Ifi207	GCCTGAGCTGTGGAAGTCTCCCCCTGAGCTGTGGAAGTCTC	GCCTGAGCTGTGGAAGTCTC	1: 173,735,157 (GRCm38)		probably benign	Het
Igf1r	GATGGAGC	GATGGAGCTGGATATGGAGC	7: 68,226,179 (GRCm38)		probably benign	Het
Krtap28-10	AGCCACAGCCACCACAGCCACAGCCACCAC	AGCCACAGCCACCACCGCCACAGCCACCACAGCCACAGCCACCAC	1: 83,042,258 (GRCm38)		probably benign	Het
Lmx1b	TCCATCTTGATGCCGTCCAACATCTTGATGCCGTCCA	TACATCTTGATGCCGTCCA	2: 33,640,489 (GRCm38)		probably null	Het
Mn1	CAG	CAGAAG	5: 111,419,711 (GRCm38)		probably benign	Het
Nf2	AAAAG	A	11: 4,829,936 (GRCm38)		probably null	Het
Nusap1	TTAGCAGTGAGGAGCAAGCTGAGA	TTAGCAGTGAGGAGCAAGCTGAGATACACGGTAGCAGTGAGGAGCAAGCTGAGA	2: 119,627,587 (GRCm38)		probably benign	Het
Or10j2	GTGACATC	G	1: 173,270,709 (GRCm38)		probably null	Het
Padi3	TCTCAC	TC	4: 140,792,972 (GRCm38)		probably benign	Het
Pik3c2g	G	GGAGA	6: 139,635,658 (GRCm38)		probably null	Het
Pou3f1	GGCGGCCG	GGCGGCCGCGGCCG	4: 124,657,805 (GRCm38)		probably benign	Het
Rassf6	ATTC	ATTCTGCCTCACTCATGGTCCTGTAGAGCAATGGGGCTTC	5: 90,608,939 (GRCm38)		probably benign	Het
Reep1	CC	CCCGAC	6: 71,707,968 (GRCm38)		probably null	Het
Slc12a1	ACAAACC	ACAAACCTTTGGCCACCAAACC	2: 125,154,210 (GRCm38)		probably benign	Het
Smpx	CCCCCCA	C	X: 157,720,923 (GRCm38)		probably benign	Het
Spaca1	TCGC	TCGCTCACGC	4: 34,049,854 (GRCm38)		probably benign	Het
Supt20	GCAGCA	GCAGCACCAGCA	3: 54,727,666 (GRCm38)		probably benign	Het
Tgoln1	GCTTGCCAGAAT	GCTTGCCAGAATCACCTCCCGTGGTCTTGCCAGAAT	6: 72,616,063 (GRCm38)		probably benign	Het
Tgoln1	T	TCACCTCCCGTGGGCTTGCCAGAAG	6: 72,616,074 (GRCm38)		probably benign	Het
Triobp	CCCCAGGACTCCCTGTGCCCAACGGGACAATCCCAGG	CCCCAGGACTCCCTGTGCCCAACGGAACAATCCCAGGACTCCCTGTGCCCAACGGGACAATCCCAGG	15: 78,967,036 (GRCm38)		probably benign	Het
Vat1l	C	T	8: 114,289,329 (GRCm38)	L320F	probably damaging	Het
Zfhx3	CAGCAACAG	CAGCAACAGAAGCAACAG	8: 108,956,092 (GRCm38)		probably benign	Het

Other mutations in Med12l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Med12l	APN	3	59,042,336 (GRCm38)	missense	probably damaging	0.98
IGL00561:Med12l	APN	3	59,227,824 (GRCm38)	missense	probably benign
IGL00974:Med12l	APN	3	59,083,014 (GRCm38)	missense	probably damaging	1.00
IGL01024:Med12l	APN	3	59,073,341 (GRCm38)	missense	probably damaging	1.00
IGL01094:Med12l	APN	3	59,093,655 (GRCm38)	missense	probably damaging	0.99
IGL01134:Med12l	APN	3	59,042,275 (GRCm38)	missense	possibly damaging	0.91
IGL01535:Med12l	APN	3	59,262,259 (GRCm38)	missense	probably damaging	1.00
IGL01653:Med12l	APN	3	59,261,893 (GRCm38)	missense	probably damaging	1.00
IGL01735:Med12l	APN	3	59,263,254 (GRCm38)	missense	probably damaging	1.00
IGL01972:Med12l	APN	3	59,261,893 (GRCm38)	missense	probably damaging	1.00
IGL02005:Med12l	APN	3	59,244,947 (GRCm38)	missense	probably damaging	1.00
IGL02098:Med12l	APN	3	59,275,855 (GRCm38)	missense	possibly damaging	0.92
IGL02115:Med12l	APN	3	59,068,319 (GRCm38)	missense	probably benign	0.00
IGL02231:Med12l	APN	3	59,245,882 (GRCm38)	missense	probably damaging	1.00
IGL02259:Med12l	APN	3	59,245,843 (GRCm38)	missense	probably damaging	1.00
IGL02369:Med12l	APN	3	59,257,373 (GRCm38)	missense	probably benign	0.00
IGL02424:Med12l	APN	3	59,092,722 (GRCm38)	missense	probably benign	0.21
IGL02501:Med12l	APN	3	59,261,976 (GRCm38)	missense	possibly damaging	0.71
IGL02525:Med12l	APN	3	59,068,368 (GRCm38)	missense	probably benign	0.01
IGL02530:Med12l	APN	3	59,077,089 (GRCm38)	missense	probably damaging	1.00
IGL02735:Med12l	APN	3	59,093,646 (GRCm38)	missense	probably damaging	1.00
IGL02865:Med12l	APN	3	59,294,292 (GRCm38)	missense	probably damaging	1.00
IGL03183:Med12l	APN	3	59,037,555 (GRCm38)	splice site	probably null
IGL03264:Med12l	APN	3	59,301,367 (GRCm38)	nonsense	probably null
FR4304:Med12l	UTSW	3	59,275,982 (GRCm38)	small insertion	probably benign
FR4340:Med12l	UTSW	3	59,275,985 (GRCm38)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,275,994 (GRCm38)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,275,988 (GRCm38)	small insertion	probably benign
FR4449:Med12l	UTSW	3	59,275,963 (GRCm38)	nonsense	probably null
FR4548:Med12l	UTSW	3	59,275,982 (GRCm38)	small insertion	probably benign
FR4589:Med12l	UTSW	3	59,275,956 (GRCm38)	small insertion	probably benign
FR4976:Med12l	UTSW	3	59,275,977 (GRCm38)	small insertion	probably benign
P0007:Med12l	UTSW	3	59,091,395 (GRCm38)	splice site	probably benign
P0045:Med12l	UTSW	3	59,091,535 (GRCm38)	missense	probably damaging	0.99
R0030:Med12l	UTSW	3	59,248,655 (GRCm38)	missense	probably damaging	1.00
R0030:Med12l	UTSW	3	59,248,655 (GRCm38)	missense	probably damaging	1.00
R0148:Med12l	UTSW	3	59,037,654 (GRCm38)	missense	probably damaging	1.00
R0325:Med12l	UTSW	3	59,077,059 (GRCm38)	missense	possibly damaging	0.88
R0330:Med12l	UTSW	3	59,227,702 (GRCm38)	missense	probably damaging	1.00
R0388:Med12l	UTSW	3	59,093,504 (GRCm38)	splice site	probably benign
R0542:Med12l	UTSW	3	59,042,401 (GRCm38)	missense	probably damaging	1.00
R0624:Med12l	UTSW	3	59,037,702 (GRCm38)	nonsense	probably null
R0625:Med12l	UTSW	3	59,247,437 (GRCm38)	missense	probably damaging	1.00
R0671:Med12l	UTSW	3	59,264,929 (GRCm38)	missense	probably damaging	1.00
R0706:Med12l	UTSW	3	59,261,980 (GRCm38)	missense	probably damaging	1.00
R0785:Med12l	UTSW	3	59,260,832 (GRCm38)	missense	probably damaging	1.00
R1054:Med12l	UTSW	3	59,248,651 (GRCm38)	missense	probably damaging	0.99
R1102:Med12l	UTSW	3	59,244,836 (GRCm38)	missense	probably damaging	0.99
R1391:Med12l	UTSW	3	59,037,738 (GRCm38)	missense	probably benign	0.00
R1501:Med12l	UTSW	3	59,260,835 (GRCm38)	critical splice donor site	probably null
R1544:Med12l	UTSW	3	59,265,240 (GRCm38)	missense	possibly damaging	0.71
R1662:Med12l	UTSW	3	59,093,617 (GRCm38)	missense	probably damaging	1.00
R1670:Med12l	UTSW	3	59,275,958 (GRCm38)	small insertion	probably benign
R1839:Med12l	UTSW	3	59,068,319 (GRCm38)	missense	probably benign
R1854:Med12l	UTSW	3	59,260,772 (GRCm38)	missense	probably damaging	1.00
R2045:Med12l	UTSW	3	59,262,310 (GRCm38)	nonsense	probably null
R2070:Med12l	UTSW	3	59,244,905 (GRCm38)	missense	probably damaging	1.00
R2132:Med12l	UTSW	3	59,265,282 (GRCm38)	splice site	probably null
R2290:Med12l	UTSW	3	59,244,938 (GRCm38)	missense	probably damaging	1.00
R2325:Med12l	UTSW	3	59,232,454 (GRCm38)	missense	probably damaging	0.99
R2352:Med12l	UTSW	3	59,240,692 (GRCm38)	missense	probably damaging	1.00
R2484:Med12l	UTSW	3	59,297,838 (GRCm38)	missense	probably benign	0.18
R2906:Med12l	UTSW	3	59,257,082 (GRCm38)	missense	probably damaging	1.00
R3735:Med12l	UTSW	3	59,091,495 (GRCm38)	missense	probably damaging	1.00
R3736:Med12l	UTSW	3	59,091,495 (GRCm38)	missense	probably damaging	1.00
R3774:Med12l	UTSW	3	59,247,942 (GRCm38)	missense	probably damaging	0.97
R3957:Med12l	UTSW	3	59,073,168 (GRCm38)	missense	probably damaging	0.99
R4020:Med12l	UTSW	3	59,247,942 (GRCm38)	missense	probably damaging	0.97
R4087:Med12l	UTSW	3	59,297,921 (GRCm38)	missense	probably benign	0.00
R4231:Med12l	UTSW	3	59,257,223 (GRCm38)	splice site	probably null
R4233:Med12l	UTSW	3	59,257,223 (GRCm38)	splice site	probably null
R4235:Med12l	UTSW	3	59,257,223 (GRCm38)	splice site	probably null
R4236:Med12l	UTSW	3	59,257,223 (GRCm38)	splice site	probably null
R4327:Med12l	UTSW	3	59,265,267 (GRCm38)	missense	probably benign	0.01
R4328:Med12l	UTSW	3	59,265,267 (GRCm38)	missense	probably benign	0.01
R4346:Med12l	UTSW	3	59,031,555 (GRCm38)	missense	probably damaging	1.00
R4543:Med12l	UTSW	3	59,091,508 (GRCm38)	missense	probably damaging	1.00
R4559:Med12l	UTSW	3	59,007,102 (GRCm38)	critical splice donor site	probably null
R4776:Med12l	UTSW	3	59,233,212 (GRCm38)	missense	probably damaging	1.00
R4877:Med12l	UTSW	3	59,244,793 (GRCm38)	missense	probably damaging	1.00
R4983:Med12l	UTSW	3	59,261,929 (GRCm38)	missense	probably damaging	1.00
R5114:Med12l	UTSW	3	59,259,688 (GRCm38)	missense	possibly damaging	0.85
R5125:Med12l	UTSW	3	59,267,214 (GRCm38)	missense	possibly damaging	0.83
R5230:Med12l	UTSW	3	59,245,788 (GRCm38)	missense	probably damaging	1.00
R5407:Med12l	UTSW	3	59,258,201 (GRCm38)	missense	probably damaging	1.00
R5426:Med12l	UTSW	3	59,248,722 (GRCm38)	missense	probably damaging	0.98
R5439:Med12l	UTSW	3	59,263,213 (GRCm38)	missense	probably null	1.00
R5449:Med12l	UTSW	3	59,259,706 (GRCm38)	missense	probably damaging	1.00
R5596:Med12l	UTSW	3	59,252,350 (GRCm38)	missense	probably benign	0.45
R5716:Med12l	UTSW	3	59,301,377 (GRCm38)	critical splice donor site	probably null
R5833:Med12l	UTSW	3	59,265,226 (GRCm38)	missense	possibly damaging	0.95
R5883:Med12l	UTSW	3	59,091,468 (GRCm38)	missense	probably damaging	1.00
R6264:Med12l	UTSW	3	59,256,002 (GRCm38)	missense	probably damaging	1.00
R6269:Med12l	UTSW	3	59,227,822 (GRCm38)	missense	probably damaging	1.00
R6394:Med12l	UTSW	3	59,235,087 (GRCm38)	missense	probably damaging	1.00
R6400:Med12l	UTSW	3	59,247,911 (GRCm38)	missense	probably damaging	1.00
R6475:Med12l	UTSW	3	59,257,079 (GRCm38)	missense	probably damaging	1.00
R6489:Med12l	UTSW	3	59,257,407 (GRCm38)	missense	probably damaging	0.99
R6654:Med12l	UTSW	3	59,262,292 (GRCm38)	missense	probably damaging	1.00
R6881:Med12l	UTSW	3	59,267,165 (GRCm38)	missense	probably benign	0.00
R7110:Med12l	UTSW	3	59,262,224 (GRCm38)	missense	possibly damaging	0.92
R7134:Med12l	UTSW	3	59,093,759 (GRCm38)	nonsense	probably null
R7137:Med12l	UTSW	3	59,258,254 (GRCm38)	missense	probably damaging	1.00
R7159:Med12l	UTSW	3	59,276,017 (GRCm38)	missense	probably benign
R7341:Med12l	UTSW	3	59,042,403 (GRCm38)	missense	possibly damaging	0.53
R7349:Med12l	UTSW	3	59,258,325 (GRCm38)	missense	probably damaging	1.00
R7413:Med12l	UTSW	3	59,091,550 (GRCm38)	missense	probably benign	0.00
R7495:Med12l	UTSW	3	59,244,773 (GRCm38)	missense	probably damaging	1.00
R7678:Med12l	UTSW	3	59,076,720 (GRCm38)	missense	probably damaging	1.00
R7697:Med12l	UTSW	3	59,240,657 (GRCm38)	missense	probably damaging	1.00
R7714:Med12l	UTSW	3	59,093,586 (GRCm38)	missense	probably benign	0.17
R7725:Med12l	UTSW	3	59,255,992 (GRCm38)	missense	probably damaging	1.00
R7846:Med12l	UTSW	3	59,264,934 (GRCm38)	missense	probably damaging	1.00
R7852:Med12l	UTSW	3	59,247,911 (GRCm38)	missense	probably damaging	1.00
R8080:Med12l	UTSW	3	59,265,186 (GRCm38)	missense	probably damaging	1.00
R8181:Med12l	UTSW	3	59,261,968 (GRCm38)	missense	probably damaging	1.00
R8223:Med12l	UTSW	3	59,086,363 (GRCm38)	missense	possibly damaging	0.79
R8560:Med12l	UTSW	3	59,037,605 (GRCm38)	missense	probably damaging	1.00
R8708:Med12l	UTSW	3	59,252,330 (GRCm38)	missense	probably benign	0.00
R8865:Med12l	UTSW	3	59,071,882 (GRCm38)	missense	probably benign
R8947:Med12l	UTSW	3	59,077,022 (GRCm38)	splice site	probably benign
R8976:Med12l	UTSW	3	59,275,908 (GRCm38)	missense	probably damaging	0.99
R9016:Med12l	UTSW	3	59,255,873 (GRCm38)	missense	probably damaging	0.96
R9183:Med12l	UTSW	3	59,077,077 (GRCm38)	missense	probably damaging	1.00
R9487:Med12l	UTSW	3	59,247,932 (GRCm38)	missense	probably benign
R9526:Med12l	UTSW	3	59,076,786 (GRCm38)	missense	probably damaging	0.96
R9802:Med12l	UTSW	3	59,261,925 (GRCm38)	missense	probably damaging	1.00
RF004:Med12l	UTSW	3	59,275,969 (GRCm38)	small insertion	probably benign
RF011:Med12l	UTSW	3	59,275,980 (GRCm38)	small insertion	probably benign
RF013:Med12l	UTSW	3	59,275,966 (GRCm38)	small insertion	probably benign
RF020:Med12l	UTSW	3	59,275,958 (GRCm38)	small insertion	probably benign
RF021:Med12l	UTSW	3	59,073,290 (GRCm38)	missense	probably benign	0.19
RF027:Med12l	UTSW	3	59,275,981 (GRCm38)	small insertion	probably benign
RF027:Med12l	UTSW	3	59,275,967 (GRCm38)	small insertion	probably benign
RF030:Med12l	UTSW	3	59,275,989 (GRCm38)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,275,989 (GRCm38)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,275,985 (GRCm38)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,275,995 (GRCm38)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,275,987 (GRCm38)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,275,981 (GRCm38)	small insertion	probably benign
RF037:Med12l	UTSW	3	59,275,956 (GRCm38)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,275,989 (GRCm38)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,275,967 (GRCm38)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,275,995 (GRCm38)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,275,985 (GRCm38)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,275,981 (GRCm38)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,275,967 (GRCm38)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,275,956 (GRCm38)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,275,995 (GRCm38)	small insertion	probably benign
RF049:Med12l	UTSW	3	59,275,969 (GRCm38)	small insertion	probably benign
RF050:Med12l	UTSW	3	59,275,973 (GRCm38)	small insertion	probably benign
RF053:Med12l	UTSW	3	59,275,993 (GRCm38)	small insertion	probably benign
RF055:Med12l	UTSW	3	59,275,983 (GRCm38)	small insertion	probably benign
RF056:Med12l	UTSW	3	59,275,993 (GRCm38)	small insertion	probably benign
RF057:Med12l	UTSW	3	59,275,980 (GRCm38)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,275,973 (GRCm38)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,275,958 (GRCm38)	small insertion	probably benign
X0062:Med12l	UTSW	3	59,233,179 (GRCm38)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	59,296,117 (GRCm38)	missense	probably benign	0.00
Z1176:Med12l	UTSW	3	59,244,943 (GRCm38)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	59,091,417 (GRCm38)	missense	probably damaging	0.98
Z1177:Med12l	UTSW	3	59,247,875 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCTAGGTGGCTCCCGATTG -3'
(R):5'- AGTCCTTAGCACTTTGGGCTTC -3'

Sequencing Primer
(F):5'- TCCCGATTGGACCCTGC -3'
(R):5'- GGGCTTCTTCCCAAGATCAATAATG -3'

Posted On

2019-12-04