Mutagenetix > Incidental Mutations

Incidental Mutation 'RF034:Olfr850'

604495

Institutional Source

Beutler Lab

Gene Symbol

Olfr850

Ensembl Gene

ENSMUSG00000094535

Gene Name

olfactory receptor 850

Synonyms

GA_x6K02T2PVTD-13214162-13213224, MOR147-2

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.191) question?

Stock #

RF034 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19477301-19478248 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 19477632 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Glutamic Acid at position 206 (A206E)

Ref Sequence

ENSEMBL: ENSMUSP00000076569 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077347] [ENSMUST00000211832]

AlphaFold

Q8VFF2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000077347
AA Change: A206E

PolyPhen 2 Score 0.460 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000076569
Gene: ENSMUSG00000094535
AA Change: A206E

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
Pfam:7tm_4	34	311	1.8e-51	PFAM
Pfam:7TM_GPCR_Srsx	38	304	1e-6	PFAM
Pfam:7tm_1	44	293	5.1e-24	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000211832
AA Change: A203E

PolyPhen 2 Score 0.460 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Predicted Effect	Zygosity
4932438A13Rik	T	TTATTATGATTATTAC	3: 37,050,760	probably benign	Het
A030005L19Rik	TGTGGCTGC	TGTGGCTGCGGTGGCTGC	1: 82,913,580	probably benign	Het
Acap3	GCATCCTGGGCTGCT	GCATCCTGGGCTGCTACATCCTGGGCTGCT	4: 155,905,092	probably benign	Het
Alpk3	GAGAAGGCAC	G	7: 81,092,414	probably benign	Het
Cox7a2l	GGA	GGATGGGGA	17: 83,502,722	probably benign	Het
Cpne1	TCCAC	TC	2: 156,073,510	probably benign	Het
Cyb5r4	CCCAGGGATGTGACAGACACACTG	CCCAGGGATGTGACAGACACACTGACCAGGGATGTGACAGACACACTG	9: 87,040,417	probably benign	Het
Cyb5r4	GA	GATGTGACAGACACACTGCCCAGGTA	9: 87,040,447	probably null	Het
Defb22	TGCGGCA	TGCGGCAGGGCTGGCCTTTGCGGCA	2: 152,485,832	probably benign	Het
Dnmt1	GGGCGGAGCACAGTTCCTACCTCGTT	GGGCGGAGCACAGTTCCTACCTCGTTTTGGTGGCGGAGCACAGTTCCTACCTCGTT	9: 20,910,120	probably null	Het
Fam71e1	GGGTCTGAGGGAGGA	GGGTCTGAGGGAGGAAGGCTGGATCCTGGATACCTAGGTCTGAGGGAGGA	7: 44,500,523	probably null	Het
Fbrsl1	GTG	GTGCGTGTGCTGTTG	5: 110,378,149	probably benign	Het
Frem3	GATC	GATCATC	8: 80,615,238	probably benign	Het
Gabre	GCTC	GCTCTGTCTC	X: 72,270,762	probably benign	Het
Gm15155	CAA	CAACAACAAA	X: 156,345,640	probably null	Het
Igf1r	GGAGATGGAGC	GGAGATGGAGCTAGAGATGGAGC	7: 68,226,176	probably benign	Het
Krtap28-10	CACCACAGC	CACCACAGCCACAGCGACCACAGC	1: 83,042,282	probably benign	Het
Mamld1	AGC	AGCGGC	X: 71,118,835	probably benign	Het
Map1a	CCAGCTCCAGCTCCA	CCAGCTCCAGCTCCAGCTACAGCTCCAGATACAGCTCCAGCTCCA	2: 121,306,304	probably benign	Het
Map1a	GCTCCAGCTCCA	GCTCCAGCTCCAGCTCCAGCTCCAGCTCCATCTCCAGCTCCA	2: 121,306,307	probably benign	Het
Neu1	TCTTCTA	T	17: 34,932,558	probably benign	Het
Nolc1	CAGCAGC	CAGCAGCAGAAGCAGC	19: 46,081,371	probably benign	Het
Pdik1l	TTTT	TTTTGTTTTTGGTTT	4: 134,279,374	probably null	Het
Rassf6	CACTCATGGTCCTGTAGAGCAATGGGGATTC	CACTCATGGTCCTGTAGAGCAATGGGGATTCTGCCTGACTCATGGTCCTGTAGAGCAATGGGGATTC	5: 90,608,912	probably benign	Het
Rassf6	ATGGTCCTGTAGAGCAATGGGGATTC	ATGGTCCTGTAGAGCAATGGGGATTCTGCCTCACTCGTGGTCCTGTAGAGCAATGGGGATTC	5: 90,608,917	probably benign	Het
Rassf6	CTGTAGAGCAATGGGGATTC	CTGTAGAGCAATGGGGATTCTGCCTCACTCATGGTCATGTAGAGCAATGGGGATTC	5: 90,608,923	probably benign	Het
Rpgrip1	AGAGGAGGA	AGA	14: 52,149,526	probably benign	Het
Rprd2	AGAGCCTGTGGTGCTCGCAGGGGAGGCTGTGGTGCTC	AGAGGCTGTGGTGCTC	3: 95,766,320	probably benign	Het
Rsf1	CG	CGAGG	7: 97,579,908	probably benign	Het
Rtbdn	GCGGC	GCGGCATCGGC	8: 84,956,175	probably benign	Het
Smarca2	CA	CACCAAGA	19: 26,631,011	probably benign	Het
Tfeb	GCA	GCACCA	17: 47,786,097	probably benign	Het
Tfeb	CAG	CAGTAG	17: 47,786,098	probably null	Het
Tmed6	AGC	AGCTGGC	8: 107,061,596	probably null	Het
Tomm5	TCTTCCGC	TCTTCCGCAGCTTCCGC	4: 45,107,976	probably benign	Het
Trappc9	TG	TGATGCTGCTGCTGCGG	15: 72,801,298	probably benign	Het

Other mutations in Olfr850

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02723:Olfr850	APN	9	19477509	missense	probably damaging	1.00
IGL03294:Olfr850	APN	9	19477989	missense	possibly damaging	0.95
PIT4305001:Olfr850	UTSW	9	19478061	missense	probably damaging	1.00
R0364:Olfr850	UTSW	9	19477972	nonsense	probably null
R0379:Olfr850	UTSW	9	19477480	missense	possibly damaging	0.75
R0449:Olfr850	UTSW	9	19478092	missense	possibly damaging	0.89
R0682:Olfr850	UTSW	9	19477349	missense	probably benign	0.03
R0693:Olfr850	UTSW	9	19477972	nonsense	probably null
R1484:Olfr850	UTSW	9	19478127	missense	probably damaging	1.00
R1599:Olfr850	UTSW	9	19478221	missense	probably damaging	0.97
R1626:Olfr850	UTSW	9	19478199	missense	probably damaging	1.00
R1742:Olfr850	UTSW	9	19478041	missense	probably damaging	1.00
R4232:Olfr850	UTSW	9	19477726	missense	probably damaging	0.98
R4237:Olfr850	UTSW	9	19477597	missense	probably benign	0.00
R5116:Olfr850	UTSW	9	19477798	missense	possibly damaging	0.67
R5643:Olfr850	UTSW	9	19477557	missense	probably benign	0.22
R6271:Olfr850	UTSW	9	19478041	missense	probably damaging	1.00
R6815:Olfr850	UTSW	9	19477765	missense	probably benign	0.20
R7222:Olfr850	UTSW	9	19477467	missense	probably damaging	1.00
R7592:Olfr850	UTSW	9	19477832	missense	possibly damaging	0.52
R8155:Olfr850	UTSW	9	19478157	missense	probably benign	0.17
R8813:Olfr850	UTSW	9	19478181	missense	possibly damaging	0.75
R9187:Olfr850	UTSW	9	19477870	missense	probably benign
X0058:Olfr850	UTSW	9	19478223	missense	probably benign	0.10
Z1177:Olfr850	UTSW	9	19477337	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- TGCCACTGCAGTATTCCTGG -3'
(R):5'- TACACAGTCATCATGGAACCCTG -3'

Sequencing Primer
(F):5'- CACTGCAGTATTCCTGGATGAATC -3'
(R):5'- ACAGTCATCATGGAACCCTGTTTTTG -3'

Posted On

2019-12-04