Mutagenetix > Incidental Mutation

Incidental Mutation 'RF035:Zfp933'

604524

Institutional Source

Beutler Lab

Gene Symbol

Zfp933

Ensembl Gene

ENSMUSG00000059423

Gene Name

zinc finger protein 933

Synonyms

2810408P10Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

RF035 (G1)

Quality Score

214.458

Status

Not validated

Chromosome

Chromosomal Location

147907443-147932823 bp(-) (GRCm39)

Type of Mutation

makesense

DNA Base Change (assembly)

TT to TTTGCGT at 147910188 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000101343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105718] [ENSMUST00000135798]

AlphaFold

Q6PEE4

Predicted Effect

probably null
Transcript: ENSMUST00000105718

SMART Domains

Protein: ENSMUSP00000101343
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
KRAB	4	66	9.49e-16	SMART
ZnF_C2H2	131	153	3.21e-4	SMART
ZnF_C2H2	159	181	5.21e-4	SMART
ZnF_C2H2	187	209	2.12e-4	SMART
ZnF_C2H2	215	237	7.26e-3	SMART
ZnF_C2H2	243	265	6.88e-4	SMART
ZnF_C2H2	271	293	1.13e-4	SMART
ZnF_C2H2	299	321	3.95e-4	SMART
ZnF_C2H2	327	349	1.56e-2	SMART
ZnF_C2H2	355	377	1.79e-2	SMART
ZnF_C2H2	383	405	4.24e-4	SMART
ZnF_C2H2	411	433	1.22e-4	SMART
ZnF_C2H2	439	461	4.79e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135798

SMART Domains

Protein: ENSMUSP00000118300
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	8e-18	BLAST
PDB:2I13\|B	32	98	1e-12	PDB
SCOP:d1fgja_	33	98	5e-13	SMART
Blast:PHD	44	98	6e-11	BLAST

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A030005L19Rik	T	TTTGGCTGCC	1: 82,891,310 (GRCm39)		probably benign	Het
Acap3	TGCATCCTGGGCTGC	TGCATCCTGGGCTGCCGCATCCTGGGCTGC	4: 155,989,548 (GRCm39)		probably benign	Het
AI837181	G	GGCT	19: 5,475,266 (GRCm39)		probably benign	Het
Amfr	GCC	GCCGGCGCGAGCTCC	8: 94,738,920 (GRCm39)		probably benign	Het
Amot	GGAGCAGCAA	G	X: 144,233,984 (GRCm39)		probably benign	Het
Bltp1	TAT	TATTATTATTATTATGAT	3: 37,104,907 (GRCm39)		probably benign	Het
Cacna1f	GAG	GAGTAG	X: 7,486,293 (GRCm39)		probably null	Het
Calhm1	CTGTGGCTGTGGCTGTGGCTGTGG	CTGTGGCTGTGGTTGTGGCTGTGGCTGTGGCTGTGG	19: 47,129,692 (GRCm39)		probably benign	Het
Chga	G	GCAT	12: 102,527,686 (GRCm39)		probably benign	Het
E4f1	CGC	CGCTGC	17: 24,674,164 (GRCm39)		probably benign	Het
E4f1	CCG	CCGGCG	17: 24,674,169 (GRCm39)		probably benign	Het
Eps8	C	CTCAG	6: 137,494,068 (GRCm39)		probably null	Het
Exd2	GCAGCCACAGCAGCCGCAGCAGCCGCAGCCACAGCCACAGCCACAGCCACAGCCACAGC	GCAGCCACAGC	12: 80,522,674 (GRCm39)		probably benign	Het
Exd2	CAGCCAGAGC	CAGC	12: 80,522,729 (GRCm39)		probably benign	Het
Gm10447	AAAAAAAAAGAAAAA	AAAAAA	11: 53,347,165 (GRCm39)		probably benign	Het
Gm10521	CTCTCTCTCT	CTCTCTCTCTCTCT	1: 171,723,860 (GRCm39)		probably null	Het
Gm8369	TGTG	TGTGCGAGTG	19: 11,489,137 (GRCm39)		probably benign	Het
Gucy1b2	CACACACACACACACACTTAC	CAC	14: 62,646,090 (GRCm39)		probably benign	Het
Ier5l	TTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTG	2: 30,363,832 (GRCm39)		probably benign	Het
Kmt2b	TTCTCCT	TTCTCCTTCTCCT	7: 30,285,782 (GRCm39)		probably benign	Het
Krtap28-10	CAG	CAGCCAAAG	1: 83,019,867 (GRCm39)		probably benign	Het
Krtap28-10	CCACCACAGC	CCACCACAGCCACAGTCACCACAGC	1: 83,020,002 (GRCm39)		probably benign	Het
Lmx1b	TCCATCTTGATGCCGTCCAACATCTTGATGCCGTCCA	TACATCTTGATGCCGTCCA	2: 33,530,501 (GRCm39)		probably null	Het
Mamld1	GCAACA	GCAACAACA	X: 70,162,418 (GRCm39)		probably benign	Het
Mamld1	AGC	AGCGGC	X: 70,162,456 (GRCm39)		probably benign	Het
Mamld1	AGC	AGCCGC	X: 70,162,444 (GRCm39)		probably benign	Het
Manbal	CGATAGAAT	C	2: 157,237,932 (GRCm39)		probably null	Het
Map1a	GCTCCAGCTCCAGCTCCA	GCTCCAGCTCCAGCTCCAGCTCCAGCTCCATCTCCAGCTCCAGCTCCA	2: 121,136,782 (GRCm39)		probably benign	Het
Mcph1	CTCT	CTCTTCT	8: 18,702,541 (GRCm39)		probably benign	Het
Nefh	CCTCACCTGGGGACTTGG	CCTCACCTGGGGACTTGGGCTCACCTGGGGACTTGG	11: 4,891,039 (GRCm39)		probably benign	Het
Nusap1	GATACACGTTAGCAGTGAGGAGCAAGCTGA	GATACACGTTAGCAGTGAGGAGCAAGCTGATATACACGTTAGCAGTGAGGAGCAAGCTGA	2: 119,458,060 (GRCm39)		probably benign	Het
Or2t49	TGGAGGTGGATTGG	TG	11: 58,393,208 (GRCm39)		probably benign	Het
P4ha2	CCAGGTG	C	11: 54,001,061 (GRCm39)		probably benign	Het
Pdik1l	CCACCA	CCACCAACACCA	4: 134,006,821 (GRCm39)		probably benign	Het
Polr1has	CACCAC	CACCACCCCCACCACCACCACAACCAC	17: 37,275,958 (GRCm39)		probably benign	Het
Rassf6	GCCTCACTCATGGTCCTGTAGAGCAATGGGGATTC	GCCTCACTCATGGTCCTGTAGAGCAATGGGGATTCTTCCTCACTCATGGTCCTGTAGAGCAATGGGGATTC	5: 90,756,767 (GRCm39)		probably benign	Het
Rfx4	TCTCTCTCTCTCTCT	TCTCTCTCTCTCTCTCGCTCTCTCTCTCTCT	10: 84,694,344 (GRCm39)		probably benign	Het
Rgs22	GCTAAAAAAAAAAAAAAAAA	G	15: 36,010,981 (GRCm39)		probably benign	Het
Rpgrip1	AGG	AGGGGG	14: 52,386,850 (GRCm39)		probably benign	Het
Slc39a4	TGTGGTC	TGTGGTCATCATGATCACCATGGTCACCATGAGCACGGTGGTC	15: 76,499,066 (GRCm39)		probably benign	Het
Srpk2	ATCCT	AT	5: 23,730,573 (GRCm39)		probably benign	Het
Strn	AGTC	AGTCCGTGCTCCCTTACCCCAGTCCGTGCTCCCTTACCCCTGTC	17: 78,984,714 (GRCm39)		probably null	Het
Tcof1	AAGAT	AAGATTGGCCCTTTCCCAGAGATGCCCTTGGCTGCTGAGAT	18: 60,966,625 (GRCm39)		probably benign	Het
Tfeb	AGC	AGCCGC	17: 48,097,036 (GRCm39)		probably benign	Het
Tmem59	T	TGTTTGTTG	4: 107,047,729 (GRCm39)		probably benign	Het
Trav6d-5	ATTTTT	ATTTTTTTTTT	14: 53,032,791 (GRCm39)		probably benign	Het
Triobp	CAGGACTCCCTGTGCCCAACGG	CAGGACTCCCTGTGCCCAACGGAACAACCCAAGGACTCCCTGTGCCCAACGG	15: 78,851,239 (GRCm39)		probably benign	Het
Tsen2	GGA	GGATGA	6: 115,537,028 (GRCm39)		probably benign	Het
Ttf2	TTCT	TTCTTCT	3: 100,870,473 (GRCm39)		probably benign	Het
Vat1l	C	T	8: 115,016,069 (GRCm39)	L320F	probably damaging	Het
Xirp2	TT	TTTAT	2: 67,355,888 (GRCm39)		probably benign	Het
Yipf3	AGAGGA	AGA	17: 46,559,898 (GRCm39)		probably benign	Het

Other mutations in Zfp933

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00576:Zfp933	APN	4	147,910,778 (GRCm39)	missense	probably damaging	1.00
IGL03377:Zfp933	APN	4	147,913,168 (GRCm39)	missense	possibly damaging	0.65
F5770:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
FR4340:Zfp933	UTSW	4	147,910,186 (GRCm39)	frame shift	probably null
FR4548:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
R0388:Zfp933	UTSW	4	147,910,899 (GRCm39)	missense	probably benign	0.35
R0523:Zfp933	UTSW	4	147,910,919 (GRCm39)	nonsense	probably null
R0539:Zfp933	UTSW	4	147,911,005 (GRCm39)	missense	probably benign	0.08
R1672:Zfp933	UTSW	4	147,910,476 (GRCm39)	missense	probably damaging	1.00
R4049:Zfp933	UTSW	4	147,910,969 (GRCm39)	missense	probably damaging	1.00
R4895:Zfp933	UTSW	4	147,910,892 (GRCm39)	nonsense	probably null
R5133:Zfp933	UTSW	4	147,911,321 (GRCm39)	missense	probably benign
R5786:Zfp933	UTSW	4	147,912,864 (GRCm39)	splice site	probably null
R5891:Zfp933	UTSW	4	147,911,231 (GRCm39)	missense	probably benign	0.03
R6111:Zfp933	UTSW	4	147,913,217 (GRCm39)	missense	probably damaging	1.00
R6382:Zfp933	UTSW	4	147,910,325 (GRCm39)	missense	probably benign	0.07
R6968:Zfp933	UTSW	4	147,910,654 (GRCm39)	missense	probably damaging	1.00
R7195:Zfp933	UTSW	4	147,910,636 (GRCm39)	missense	probably benign	0.16
R7555:Zfp933	UTSW	4	147,910,589 (GRCm39)	missense	probably damaging	1.00
R7902:Zfp933	UTSW	4	147,911,058 (GRCm39)	missense	probably damaging	0.96
R8319:Zfp933	UTSW	4	147,912,910 (GRCm39)	missense	possibly damaging	0.87
R8688:Zfp933	UTSW	4	147,911,249 (GRCm39)	missense	probably benign	0.14
R9019:Zfp933	UTSW	4	147,911,021 (GRCm39)	missense	probably damaging	1.00
RF024:Zfp933	UTSW	4	147,910,898 (GRCm39)	missense	probably damaging	1.00
RF028:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
RF043:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
V7581:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7582:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7583:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TCTAGTCATAGACTTCCTTCCGTAG -3'
(R):5'- ACACATACTGGAGAGAAACCTTATG -3'

Sequencing Primer
(F):5'- TCAACGACTACTGGGATCTGCAG -3'
(R):5'- TGTATGTAATCAATGTGGTAAAGCC -3'

Posted On

2019-12-04