Mutagenetix > Incidental Mutation

Incidental Mutation 'RF040:Or52z15'

604769

Institutional Source

Beutler Lab

Gene Symbol

Or52z15

Ensembl Gene

ENSMUSG00000073943

Gene Name

olfactory receptor family 52 subfamily Z member 15

Synonyms

MOR31-15P, GA_x6K02T2PBJ9-6416276-6417237, Olfr625

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

RF040 (G1)

Quality Score

214.458

Status

Not validated

Chromosome

Chromosomal Location

103331936-103332889 bp(+) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

ACTTGCTGATATCTT to ACTT at 103332145 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000095797 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098195] [ENSMUST00000190218]

AlphaFold

A0A140T8L4

Predicted Effect

probably null
Transcript: ENSMUST00000098195

SMART Domains

Protein: ENSMUSP00000095797
Gene: ENSMUSG00000073943

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	317	8.1e-110	PFAM
Pfam:7TM_GPCR_Srsx	41	225	3.6e-9	PFAM
Pfam:7tm_1	47	299	1.6e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000190218

SMART Domains

Protein: ENSMUSP00000140044
Gene: ENSMUSG00000073943

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	31	214	7e-8	PFAM
Pfam:7tm_1	37	289	2.8e-26	PFAM
Pfam:7tm_4	136	282	2.7e-28	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A030005L19Rik	TGCTGTG	TGCTGTGACAGCTGTG	1: 82,891,298 (GRCm39)		probably benign	Het
A030005L19Rik	G	GTGGCTGCTC	1: 82,891,311 (GRCm39)		probably benign	Het
Brme1	TG	TGTCAGGGCAGCAGCAG	8: 84,894,204 (GRCm39)		probably benign	Het
Cacna1f	CTGAATTGGTTCCCAGACCCGTGT	CT	X: 7,485,210 (GRCm39)		probably null	Het
Calhm1	C	CTGTGGCTGTGGA	19: 47,129,716 (GRCm39)		probably benign	Het
Cdx1	CTGCTG	CTGCTGATGCTG	18: 61,152,942 (GRCm39)		probably benign	Het
Cyb5r4	ACACACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	ACACACTGCCCAGGGATGTGACAGCCACACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	9: 86,922,462 (GRCm39)		probably benign	Het
Dbr1	GAGGAG	GAGGAGTAGGAG	9: 99,465,750 (GRCm39)		probably null	Het
Dnajc2	TAGTTG	T	5: 21,962,695 (GRCm39)		probably null	Het
Fgd6	ATT	A	10: 93,880,187 (GRCm39)		probably null	Het
Flywch1	CTCCTGGTGT	CTCCTGGTGTGGGGAGGCTACGTACTCACCCAGTCCTGGTGT	17: 23,981,143 (GRCm39)		probably null	Het
Gab3	CTT	CTTTTT	X: 74,043,633 (GRCm39)		probably benign	Het
Garin5a	C	CGGGGTCAGAGGGAGGAAGGCTGGATCCTGGATACA	7: 44,149,945 (GRCm39)		probably null	Het
Garin5a	GGGAGGA	GGGAGGAAGGCTGGATCCTGGATACCTGGGTCTGATGGAGGA	7: 44,149,955 (GRCm39)		probably null	Het
Gm40460	CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 141,794,554 (GRCm39)		probably benign	Het
Gykl1	G	A	18: 52,827,488 (GRCm39)	R232Q	probably benign	Het
Heatr3	TAT	TATTGAT	8: 88,883,085 (GRCm39)		probably benign	Het
Kmt2e	TTT	TTTTCTT	5: 23,683,507 (GRCm39)		probably benign	Het
Luzp1	A	AGGTGGCCTCTTCAGC	4: 136,270,507 (GRCm39)		probably benign	Het
Mamld1	AACA	AACAACA	X: 70,162,420 (GRCm39)		probably benign	Het
Mast4	CCTCGGGGACAAGCTGTGAGTTGGGGAAC	CC	13: 102,875,749 (GRCm39)		probably benign	Het
Med12l	AGC	AGCCGC	3: 59,183,388 (GRCm39)		probably benign	Het
Med12l	GCA	GCATCA	3: 59,183,410 (GRCm39)		probably benign	Het
Mn1	CAG	CAGAAG	5: 111,567,571 (GRCm39)		probably benign	Het
Morn4	GCAGTGAG	GCAGTGAGTCAGTCAGTGAG	19: 42,064,550 (GRCm39)		probably null	Het
Ncoa6	TGCAGC	TGC	2: 155,263,651 (GRCm39)		probably benign	Het
Nlrp3	GGGTA	G	11: 59,449,378 (GRCm39)		probably null	Het
Nolc1	AGCAGCAGC	AGCAGCAGCCGCAGCAGC	19: 46,069,802 (GRCm39)		probably benign	Het
Nusap1	TTAGCAGTGAGGAGCAAGCTGAGA	TTAGCAGTGAGGAGCAAGCTGAGATACACGGTAGCAGTGAGGAGCAAGCTGAGA	2: 119,458,068 (GRCm39)		probably benign	Het
Or10j2	GTGACATC	G	1: 173,098,276 (GRCm39)		probably null	Het
Osmr	TTCT	TTCTTCT	15: 6,867,182 (GRCm39)		probably benign	Het
Padi3	TCTCAC	TC	4: 140,520,283 (GRCm39)		probably benign	Het
Pdik1l	AC	ACCACCGC	4: 134,006,826 (GRCm39)		probably benign	Het
Rpgrip1	AGGAAGAGG	AG	14: 52,386,994 (GRCm39)		probably null	Het
Rragd	CATGCCTTTCATTCTA	C	4: 32,995,150 (GRCm39)		probably benign	Het
Ryr3	CTGA	C	2: 112,740,869 (GRCm39)		probably benign	Het
Sh3pxd2b	CCTGTG	CCTGTGTCTGTG	11: 32,373,055 (GRCm39)		probably benign	Het
Slc39a4	TGTGGTC	TGTGGTCATCATGATCACCATGGTCACCATGATCACGGTGGTC	15: 76,499,066 (GRCm39)		probably benign	Het
Smarca2	GC	GCCCCACC	19: 26,608,422 (GRCm39)		probably benign	Het
Spata31f3	TCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGTCATTCAACACTTTGG	TCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGTCATTCAACACTTTGG	4: 42,871,823 (GRCm39)		probably benign	Het
Sprr2b	CAGTATGCTGTGAGCCTTGTCCTCCT	C	3: 92,224,871 (GRCm39)		probably null	Het
Sry	GCTG	GCTGGTGGTGGTGGTCATGGAACTG	Y: 2,662,590 (GRCm39)		probably benign	Het
Tcof1	CT	CTATT	18: 60,961,480 (GRCm39)		probably benign	Het
Tcof1	GC	GCTGCTGAGATGGGCACTTTCCCAGAGATCCCCTTGTC	18: 60,966,655 (GRCm39)		probably benign	Het
Tfeb	AGC	AGCCGC	17: 48,097,036 (GRCm39)		probably benign	Het
Tfeb	GCA	GCATCA	17: 48,097,037 (GRCm39)		probably benign	Het
Tfeb	GCA	GCAACA	17: 48,097,022 (GRCm39)		probably benign	Het
Tfeb	CAG	CAGGAG	17: 48,097,035 (GRCm39)		probably benign	Het
Tgoln1	T	TCACCTCCCGTGGTCTTGCCAGAAG	6: 72,593,057 (GRCm39)		probably benign	Het
Tmem59	TTTGTTT	TTTGTTTGGTTGTTT	4: 107,047,723 (GRCm39)		probably benign	Het
Trappc9	TGCTGCTGCTGCTGCTGCTGCTGCTGCTGC	TGCTGCTGCTGCTGCGGCTGCTGCTGCTGCTGCTGCTGCTGCTGC	15: 72,673,141 (GRCm39)		probably benign	Het
Triobp	CAA	CAACCCCAGGACTCCCTGTGCCCAACGGGGGAA	15: 78,851,263 (GRCm39)		probably benign	Het
Xirp2	TT	TTTAT	2: 67,355,888 (GRCm39)		probably benign	Het
Zfhx3	CAGCA	CAGCAACAGGAGCA	8: 109,682,733 (GRCm39)		probably benign	Het

Other mutations in Or52z15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02547:Or52z15	APN	7	103,331,973 (GRCm39)	missense	probably benign	0.01
R1160:Or52z15	UTSW	7	103,332,068 (GRCm39)	missense	possibly damaging	0.50
R1508:Or52z15	UTSW	7	103,332,678 (GRCm39)	missense	possibly damaging	0.50
R1766:Or52z15	UTSW	7	103,332,068 (GRCm39)	missense	possibly damaging	0.50
R1901:Or52z15	UTSW	7	103,332,750 (GRCm39)	missense	probably damaging	0.98
R2116:Or52z15	UTSW	7	103,332,519 (GRCm39)	missense	probably damaging	1.00
R4701:Or52z15	UTSW	7	103,332,269 (GRCm39)	missense	probably damaging	1.00
R4995:Or52z15	UTSW	7	103,332,574 (GRCm39)	missense	probably damaging	1.00
R5198:Or52z15	UTSW	7	103,331,936 (GRCm39)	missense	probably benign	0.40
R5750:Or52z15	UTSW	7	103,332,362 (GRCm39)	missense	possibly damaging	0.95
R5776:Or52z15	UTSW	7	103,332,246 (GRCm39)	missense	probably damaging	1.00
R5905:Or52z15	UTSW	7	103,332,781 (GRCm39)	missense	probably damaging	1.00
R5933:Or52z15	UTSW	7	103,332,680 (GRCm39)	missense	probably damaging	1.00
R6488:Or52z15	UTSW	7	103,332,285 (GRCm39)	missense	probably damaging	0.98
R7846:Or52z15	UTSW	7	103,332,407 (GRCm39)	missense	probably benign	0.03
R7878:Or52z15	UTSW	7	103,332,471 (GRCm39)	missense	probably damaging	1.00
R9170:Or52z15	UTSW	7	103,332,404 (GRCm39)	missense	probably benign	0.00
R9347:Or52z15	UTSW	7	103,332,464 (GRCm39)	missense	probably damaging	0.97
R9474:Or52z15	UTSW	7	103,332,477 (GRCm39)	missense	probably damaging	1.00
R9781:Or52z15	UTSW	7	103,332,246 (GRCm39)	missense	probably damaging	1.00
Z1088:Or52z15	UTSW	7	103,332,393 (GRCm39)	missense	probably benign	0.01
Z1176:Or52z15	UTSW	7	103,332,312 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- GTCAGAGATATCTGGTACACCATG -3'
(R):5'- TGCAATGCCCATTTTGATGATG -3'

Sequencing Primer
(F):5'- GAGATATCTGGTACACCATGATTGG -3'
(R):5'- TCTTAGTGGATAACAGATGGCCAC -3'

Posted On

2019-12-04