Mutagenetix > Incidental Mutation

Incidental Mutation 'RF043:Zfp933'

604909

Institutional Source

Beutler Lab

Gene Symbol

Zfp933

Ensembl Gene

ENSMUSG00000059423

Gene Name

zinc finger protein 933

Synonyms

2810408P10Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

RF043 (G1)

Quality Score

215.098

Status

Not validated

Chromosome

Chromosomal Location

147907443-147932823 bp(-) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

TT to TTTGCCT at 147910188 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000101343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105718] [ENSMUST00000135798]

AlphaFold

Q6PEE4

Predicted Effect

probably null
Transcript: ENSMUST00000105718

SMART Domains

Protein: ENSMUSP00000101343
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
KRAB	4	66	9.49e-16	SMART
ZnF_C2H2	131	153	3.21e-4	SMART
ZnF_C2H2	159	181	5.21e-4	SMART
ZnF_C2H2	187	209	2.12e-4	SMART
ZnF_C2H2	215	237	7.26e-3	SMART
ZnF_C2H2	243	265	6.88e-4	SMART
ZnF_C2H2	271	293	1.13e-4	SMART
ZnF_C2H2	299	321	3.95e-4	SMART
ZnF_C2H2	327	349	1.56e-2	SMART
ZnF_C2H2	355	377	1.79e-2	SMART
ZnF_C2H2	383	405	4.24e-4	SMART
ZnF_C2H2	411	433	1.22e-4	SMART
ZnF_C2H2	439	461	4.79e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000116174

Predicted Effect

probably benign
Transcript: ENSMUST00000135798

SMART Domains

Protein: ENSMUSP00000118300
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	8e-18	BLAST
PDB:2I13\|B	32	98	1e-12	PDB
SCOP:d1fgja_	33	98	5e-13	SMART
Blast:PHD	44	98	6e-11	BLAST

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Anapc2	GCGGCGGCGGCGAC	GC	2: 25,162,573 (GRCm39)		probably benign	Het
Ankhd1	CGGCGG	CGGCGGTGGCGG	18: 36,693,970 (GRCm39)		probably benign	Het
Btnl10	CAAA	CAAAAAA	11: 58,814,752 (GRCm39)		probably benign	Het
Cpne1	TCCAC	TC	2: 155,915,430 (GRCm39)		probably benign	Het
Cyb5r4	ACACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	ACACTGCCCAGGGATGTGACAGACGCACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	9: 86,922,464 (GRCm39)		probably benign	Het
Cyb5r4	AGACACACTGCCCAGGGA	AGACACACTGCCCAGGGATGTGACCGACACACTGCCCAGGGA	9: 86,922,484 (GRCm39)		probably benign	Het
Dbr1	GAGGAG	GAGGAGCAGGAG	9: 99,465,750 (GRCm39)		probably benign	Het
Defb22	GCGGCA	GCGGCAGAGCTGGCCTTTGCGGCA	2: 152,327,753 (GRCm39)		probably benign	Het
Emid1	G	T	11: 5,094,322 (GRCm39)	P63Q	probably damaging	Het
Gabre	GCTCCG	GCTCCGACTCCG	X: 71,313,654 (GRCm39)		probably benign	Het
Gar1	AACTGCC	A	3: 129,624,337 (GRCm39)		probably benign	Het
Gm10181	GAGAGAGAGAGAGA	G	9: 25,000,755 (GRCm39)		probably null	Het
Hic1	GGGA	G	11: 75,060,281 (GRCm39)		probably benign	Het
Il2	GG	GGGCTTGAAGTGTG	3: 37,179,991 (GRCm39)		probably benign	Het
Iqcf4	CTTTTCCTTTT	CTTTTCCTTTTCCTTTTCCTTTTCCTTTTCCTTTTCGTTTTCCTTTT	9: 106,447,812 (GRCm39)		probably benign	Het
Irag2	TG	TGAGCACATGG	6: 145,119,516 (GRCm39)		probably benign	Het
Krtap4-2	AGGGGCGGCAGC	A	11: 99,525,547 (GRCm39)		probably null	Het
Lmx1b	CATCTTGATGCCGTCCAA	CA	2: 33,530,521 (GRCm39)		probably null	Het
Lrch1	TGGTGGTG	T	14: 75,185,015 (GRCm39)		probably null	Het
Lypd8	CA	CAGTTCCCTCGCCTCTGTTACCCCACAAATCACCAAGA	11: 58,281,069 (GRCm39)		probably benign	Het
Mamld1	AGC	AGCTGC	X: 70,162,441 (GRCm39)		probably benign	Het
Mrgprx1	GAAC	GAACAAC	7: 47,671,257 (GRCm39)		probably benign	Het
Nefh	CTTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGA	CTTGGCCTCACCTGGGGAATTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGA	11: 4,891,016 (GRCm39)		probably benign	Het
Nusap1	AGTGAGGAGCAAGCTGAGA	AGTGAGGAGCAAGCTGAGATACACGTTAGCCGTGAGGAGCAAGCTGAGA	2: 119,458,073 (GRCm39)		probably benign	Het
P4ha2	GGTGTTG	GG	11: 54,001,076 (GRCm39)		probably null	Het
Padi3	TCTCAC	TC	4: 140,520,283 (GRCm39)		probably benign	Het
Plekhs1	TCCAGAC	TCCAGACCTCCCCCCAGAC	19: 56,468,290 (GRCm39)		probably benign	Het
Ptms	TCCTCCTC	TCCTCCTCCTC	6: 124,891,411 (GRCm39)		probably benign	Het
Rassf6	AATGGGGATTC	AATGGGGATTCTGCCTCACTCATGGTCCTGTAGAGCCATGGGGATTC	5: 90,756,791 (GRCm39)		probably benign	Het
Rassf6	ATTC	ATTCTGCCTCACTCAGGGTCCTGTAGAGCAATGGGGCTTC	5: 90,756,798 (GRCm39)		probably benign	Het
Rgs22	CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	CAAAAAAAAAAAA	15: 36,010,982 (GRCm39)		probably benign	Het
Rnf144a	TCTC	TCTCTCTCTCTCTCGCTC	12: 26,364,013 (GRCm39)		probably benign	Het
Rpgrip1	GAG	GAGAAG	14: 52,386,852 (GRCm39)		probably benign	Het
Sgpp1	ACACAC	A	12: 75,769,399 (GRCm39)		probably null	Het
Slc39a4	GTC	GTCATCATGATCACCATGGTCACCATGATCGCTGTGTTC	15: 76,499,070 (GRCm39)		probably benign	Het
Spaca1	TCTCGC	TCTCGCGCTCGC	4: 34,049,846 (GRCm39)		probably benign	Het
Stard8	GGA	GGATGA	X: 98,110,126 (GRCm39)		probably benign	Het
Stard8	GA	GAGCA	X: 98,110,133 (GRCm39)		probably benign	Het
Tcof1	AGATCCCCTTGGC	AGATCCCCTTGGCTGCTGAGAAGGGCACTTTCCCAGGGATCCCCTTGGC	18: 60,966,644 (GRCm39)		probably benign	Het
Tgoln1	TGGGCTTG	TGGGCTTGTCAGAATCACCTCCTGCGGGCTTG	6: 72,593,019 (GRCm39)		probably benign	Het
Tgoln1	GCTTGCCAGAAT	GCTTGCCAGAATCACCGCCCGTGGTCTTGCCAGAAT	6: 72,593,046 (GRCm39)		probably benign	Het
Tmed6	AGC	AGCTCGC	8: 107,788,228 (GRCm39)		probably null	Het
Trappc9	GCTGCTGCTGCTGCTGCTGCTGCTGCT	GCTGCTGCTGCTGCTACTGCTGCTGCTGCTGCTGCTGCTGCT	15: 72,673,154 (GRCm39)		probably benign	Het

Other mutations in Zfp933

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00576:Zfp933	APN	4	147,910,778 (GRCm39)	missense	probably damaging	1.00
IGL03377:Zfp933	APN	4	147,913,168 (GRCm39)	missense	possibly damaging	0.65
F5770:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
FR4340:Zfp933	UTSW	4	147,910,186 (GRCm39)	frame shift	probably null
FR4548:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
R0388:Zfp933	UTSW	4	147,910,899 (GRCm39)	missense	probably benign	0.35
R0523:Zfp933	UTSW	4	147,910,919 (GRCm39)	nonsense	probably null
R0539:Zfp933	UTSW	4	147,911,005 (GRCm39)	missense	probably benign	0.08
R1672:Zfp933	UTSW	4	147,910,476 (GRCm39)	missense	probably damaging	1.00
R4049:Zfp933	UTSW	4	147,910,969 (GRCm39)	missense	probably damaging	1.00
R4895:Zfp933	UTSW	4	147,910,892 (GRCm39)	nonsense	probably null
R5133:Zfp933	UTSW	4	147,911,321 (GRCm39)	missense	probably benign
R5786:Zfp933	UTSW	4	147,912,864 (GRCm39)	splice site	probably null
R5891:Zfp933	UTSW	4	147,911,231 (GRCm39)	missense	probably benign	0.03
R6111:Zfp933	UTSW	4	147,913,217 (GRCm39)	missense	probably damaging	1.00
R6382:Zfp933	UTSW	4	147,910,325 (GRCm39)	missense	probably benign	0.07
R6968:Zfp933	UTSW	4	147,910,654 (GRCm39)	missense	probably damaging	1.00
R7195:Zfp933	UTSW	4	147,910,636 (GRCm39)	missense	probably benign	0.16
R7555:Zfp933	UTSW	4	147,910,589 (GRCm39)	missense	probably damaging	1.00
R7902:Zfp933	UTSW	4	147,911,058 (GRCm39)	missense	probably damaging	0.96
R8319:Zfp933	UTSW	4	147,912,910 (GRCm39)	missense	possibly damaging	0.87
R8688:Zfp933	UTSW	4	147,911,249 (GRCm39)	missense	probably benign	0.14
R9019:Zfp933	UTSW	4	147,911,021 (GRCm39)	missense	probably damaging	1.00
RF024:Zfp933	UTSW	4	147,910,898 (GRCm39)	missense	probably damaging	1.00
RF028:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
RF035:Zfp933	UTSW	4	147,910,188 (GRCm39)	makesense	probably null
V7581:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7582:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7583:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GTGTTGGCTAAAGATCACACCAAAC -3'
(R):5'- GACACATACTGGAGAGAAACCTTATG -3'

Sequencing Primer
(F):5'- TCAACGACTACTGGGATCTGCAG -3'
(R):5'- TGTATGTAATCAATGTGGTAAAGCC -3'

Posted On

2019-12-04