Mutagenetix > Incidental Mutation

Incidental Mutation 'RF043:Rgs22'

604934

Institutional Source

Beutler Lab

Gene Symbol

Rgs22

Ensembl Gene

ENSMUSG00000037627

Gene Name

regulator of G-protein signalling 22

Synonyms

Accession Numbers

Genbank: NM_001195748; MGI: 3613651

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

RF043 (G1)

Quality Score

217.468

Status

Not validated

Chromosome

Chromosomal Location

36009479-36140400 bp(-) (GRCm38)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA to CAAAAAAAAAAAA at 36010836 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134259 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172831]

AlphaFold

G3UYX5

Predicted Effect

probably benign
Transcript: ENSMUST00000172831

SMART Domains

Protein: ENSMUSP00000134259
Gene: ENSMUSG00000037627

Domain	Start	End	E-Value	Type
low complexity region	9	19	N/A	INTRINSIC
low complexity region	62	76	N/A	INTRINSIC
low complexity region	173	179	N/A	INTRINSIC
low complexity region	376	391	N/A	INTRINSIC
RGS	845	973	3.15e-2	SMART
RGS	1014	1134	1.56e-15	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000173018

SMART Domains

Protein: ENSMUSP00000133703
Gene: ENSMUSG00000037627

Domain	Start	End	E-Value	Type
RGS	4	124	1.56e-15	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.4%
20x: 98.7%

Validation Efficiency

Allele List at MGI

All alleles(2) : Targeted, other(2)

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Anapc2	GCGGCGGCGGCGAC	GC	2: 25,272,561		probably benign	Het
Ankhd1	CGGCGG	CGGCGGTGGCGG	18: 36,560,917		probably benign	Het
Btnl10	CAAA	CAAAAAA	11: 58,923,926		probably benign	Het
Cpne1	TCCAC	TC	2: 156,073,510		probably benign	Het
Cyb5r4	ACACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	ACACTGCCCAGGGATGTGACAGACGCACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA	9: 87,040,411		probably benign	Het
Cyb5r4	AGACACACTGCCCAGGGA	AGACACACTGCCCAGGGATGTGACCGACACACTGCCCAGGGA	9: 87,040,431		probably benign	Het
Dbr1	GAGGAG	GAGGAGCAGGAG	9: 99,583,697		probably benign	Het
Defb22	GCGGCA	GCGGCAGAGCTGGCCTTTGCGGCA	2: 152,485,833		probably benign	Het
Emid1	G	T	11: 5,144,322	P63Q	probably damaging	Het
Gabre	GCTCCG	GCTCCGACTCCG	X: 72,270,048		probably benign	Het
Gar1	AACTGCC	A	3: 129,830,688		probably benign	Het
Gm10181	GAGAGAGAGAGAGA	G	9: 25,089,459		probably null	Het
Hic1	GGGA	G	11: 75,169,455		probably benign	Het
Il2	GG	GGGCTTGAAGTGTG	3: 37,125,842		probably benign	Het
Iqcf4	CTTTTCCTTTT	CTTTTCCTTTTCCTTTTCCTTTTCCTTTTCCTTTTCGTTTTCCTTTT	9: 106,570,613		probably benign	Het
Krtap4-2	AGGGGCGGCAGC	A	11: 99,634,721		probably null	Het
Lmx1b	CATCTTGATGCCGTCCAA	CA	2: 33,640,509		probably null	Het
Lrch1	TGGTGGTG	T	14: 74,947,575		probably null	Het
Lrmp	TG	TGAGCACATGG	6: 145,173,790		probably benign	Het
Lypd8	CA	CAGTTCCCTCGCCTCTGTTACCCCACAAATCACCAAGA	11: 58,390,243		probably benign	Het
Mamld1	AGC	AGCTGC	X: 71,118,835		probably benign	Het
Mrgprx1	GAAC	GAACAAC	7: 48,021,509		probably benign	Het
Nefh	CTTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGA	CTTGGCCTCACCTGGGGAATTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGA	11: 4,941,016		probably benign	Het
Nusap1	AGTGAGGAGCAAGCTGAGA	AGTGAGGAGCAAGCTGAGATACACGTTAGCCGTGAGGAGCAAGCTGAGA	2: 119,627,592		probably benign	Het
P4ha2	GGTGTTG	GG	11: 54,110,250		probably null	Het
Padi3	TCTCAC	TC	4: 140,792,972		probably benign	Het
Plekhs1	TCCAGAC	TCCAGACCTCCCCCCAGAC	19: 56,479,858		probably benign	Het
Ptms	TCCTCCTC	TCCTCCTCCTC	6: 124,914,448		probably benign	Het
Rassf6	AATGGGGATTC	AATGGGGATTCTGCCTCACTCATGGTCCTGTAGAGCCATGGGGATTC	5: 90,608,932		probably benign	Het
Rassf6	ATTC	ATTCTGCCTCACTCAGGGTCCTGTAGAGCAATGGGGCTTC	5: 90,608,939		probably benign	Het
Rnf144a	TCTC	TCTCTCTCTCTCTCGCTC	12: 26,314,014		probably benign	Het
Rpgrip1	GAG	GAGAAG	14: 52,149,395		probably benign	Het
Sgpp1	ACACAC	A	12: 75,722,625		probably null	Het
Slc39a4	GTC	GTCATCATGATCACCATGGTCACCATGATCGCTGTGTTC	15: 76,614,870		probably benign	Het
Spaca1	TCTCGC	TCTCGCGCTCGC	4: 34,049,846		probably benign	Het
Stard8	GGA	GGATGA	X: 99,066,520		probably benign	Het
Stard8	GA	GAGCA	X: 99,066,527		probably benign	Het
Tcof1	AGATCCCCTTGGC	AGATCCCCTTGGCTGCTGAGAAGGGCACTTTCCCAGGGATCCCCTTGGC	18: 60,833,572		probably benign	Het
Tgoln1	TGGGCTTG	TGGGCTTGTCAGAATCACCTCCTGCGGGCTTG	6: 72,616,036		probably benign	Het
Tgoln1	GCTTGCCAGAAT	GCTTGCCAGAATCACCGCCCGTGGTCTTGCCAGAAT	6: 72,616,063		probably benign	Het
Tmed6	AGC	AGCTCGC	8: 107,061,596		probably null	Het
Trappc9	GCTGCTGCTGCTGCTGCTGCTGCTGCT	GCTGCTGCTGCTGCTACTGCTGCTGCTGCTGCTGCTGCTGCT	15: 72,801,305		probably benign	Het
Zfp933	TT	TTTGCCT	4: 147,825,731		probably null	Het

Other mutations in Rgs22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00164:Rgs22	APN	15	36099931	missense	possibly damaging	0.93
IGL00594:Rgs22	APN	15	36083631	missense	probably benign	0.00
IGL01464:Rgs22	APN	15	36083641	missense	possibly damaging	0.90
IGL01686:Rgs22	APN	15	36103835	missense	probably benign	0.00
IGL01761:Rgs22	APN	15	36103751	missense	probably damaging	0.99
IGL02045:Rgs22	APN	15	36013154	missense	probably benign	0.33
IGL02378:Rgs22	APN	15	36103805	missense	probably benign	0.00
IGL02490:Rgs22	APN	15	36054847	missense	probably damaging	1.00
IGL03219:Rgs22	APN	15	36107048	missense	probably damaging	1.00
IGL03229:Rgs22	APN	15	36015779	splice site	probably benign
IGL03328:Rgs22	APN	15	36043204	critical splice donor site	probably null
3-1:Rgs22	UTSW	15	36100036	missense	possibly damaging	0.48
R0254:Rgs22	UTSW	15	36104552	missense	probably damaging	0.99
R0463:Rgs22	UTSW	15	36092938	missense	probably damaging	1.00
R0467:Rgs22	UTSW	15	36099795	nonsense	probably null
R0486:Rgs22	UTSW	15	36092882	missense	probably damaging	0.98
R0554:Rgs22	UTSW	15	36054709	missense	probably benign	0.10
R0602:Rgs22	UTSW	15	36139872	splice site	probably benign
R0906:Rgs22	UTSW	15	36103902	intron	probably benign
R1159:Rgs22	UTSW	15	36040693	missense	probably damaging	1.00
R1300:Rgs22	UTSW	15	36101762	missense	probably benign	0.43
R1439:Rgs22	UTSW	15	36025793	splice site	probably benign
R1491:Rgs22	UTSW	15	36092901	missense	probably damaging	0.98
R1502:Rgs22	UTSW	15	36080851	missense	probably damaging	1.00
R1514:Rgs22	UTSW	15	36013100	missense	probably benign	0.00
R1538:Rgs22	UTSW	15	36048776	missense	probably damaging	1.00
R1784:Rgs22	UTSW	15	36087436	missense	probably damaging	1.00
R1938:Rgs22	UTSW	15	36101804	missense	probably benign	0.00
R1972:Rgs22	UTSW	15	36103836	missense	probably benign	0.01
R2109:Rgs22	UTSW	15	36099734	nonsense	probably null
R2208:Rgs22	UTSW	15	36050232	missense	probably benign	0.01
R3696:Rgs22	UTSW	15	36099892	missense	probably benign	0.00
R3697:Rgs22	UTSW	15	36099892	missense	probably benign	0.00
R3698:Rgs22	UTSW	15	36099892	missense	probably benign	0.00
R3879:Rgs22	UTSW	15	36106905	missense	possibly damaging	0.52
R4080:Rgs22	UTSW	15	36107076	missense	probably damaging	1.00
R4363:Rgs22	UTSW	15	36103874	missense	probably damaging	0.99
R4591:Rgs22	UTSW	15	36100136	missense	probably benign	0.01
R4673:Rgs22	UTSW	15	36099933	missense	probably benign	0.04
R4829:Rgs22	UTSW	15	36103888	missense	probably damaging	1.00
R4831:Rgs22	UTSW	15	36050148	missense	probably benign	0.00
R4865:Rgs22	UTSW	15	36100212	missense	probably damaging	1.00
R4907:Rgs22	UTSW	15	36087424	missense	possibly damaging	0.61
R4944:Rgs22	UTSW	15	36025942	missense	possibly damaging	0.83
R4975:Rgs22	UTSW	15	36054876	nonsense	probably null
R5056:Rgs22	UTSW	15	36050245	splice site	probably null
R5126:Rgs22	UTSW	15	36040644	missense	probably damaging	0.96
R5138:Rgs22	UTSW	15	36099788	missense	probably benign	0.04
R5444:Rgs22	UTSW	15	36015627	missense	possibly damaging	0.83
R5507:Rgs22	UTSW	15	36099652	missense	probably damaging	0.99
R5640:Rgs22	UTSW	15	36106955	missense	probably benign	0.00
R5969:Rgs22	UTSW	15	36015636	missense	probably benign	0.00
R6005:Rgs22	UTSW	15	36010567	missense	probably benign	0.39
R6053:Rgs22	UTSW	15	36100007	missense	probably benign	0.04
R6134:Rgs22	UTSW	15	36107048	missense	probably damaging	1.00
R6230:Rgs22	UTSW	15	36100030	missense	probably benign	0.02
R6295:Rgs22	UTSW	15	36087374	missense	probably benign	0.00
R6352:Rgs22	UTSW	15	36092921	missense	probably damaging	1.00
R6809:Rgs22	UTSW	15	36048764	missense	probably damaging	1.00
R6900:Rgs22	UTSW	15	36010747	missense	possibly damaging	0.61
R6947:Rgs22	UTSW	15	36103890	critical splice acceptor site	probably null
R7102:Rgs22	UTSW	15	36122313	missense	probably damaging	1.00
R7126:Rgs22	UTSW	15	36103808	missense	probably damaging	0.97
R7263:Rgs22	UTSW	15	36015643	missense	possibly damaging	0.86
R7623:Rgs22	UTSW	15	36040710	missense	probably benign	0.08
R7732:Rgs22	UTSW	15	36025981	missense	probably damaging	1.00
R7748:Rgs22	UTSW	15	36122269	critical splice donor site	probably null
R7771:Rgs22	UTSW	15	36050078	missense	possibly damaging	0.94
R7835:Rgs22	UTSW	15	36081911	critical splice donor site	probably null
R7849:Rgs22	UTSW	15	36099712	missense	probably damaging	1.00
R7954:Rgs22	UTSW	15	36082002	missense	possibly damaging	0.75
R8384:Rgs22	UTSW	15	36046012	critical splice donor site	probably null
R8516:Rgs22	UTSW	15	36010335	makesense	probably null
R8904:Rgs22	UTSW	15	36025981	missense	probably damaging	1.00
R8923:Rgs22	UTSW	15	36092960	missense	probably damaging	1.00
R9287:Rgs22	UTSW	15	36098263	missense	probably damaging	1.00
R9324:Rgs22	UTSW	15	36087398	missense	probably benign
R9660:Rgs22	UTSW	15	36040710	missense	probably benign	0.08
R9679:Rgs22	UTSW	15	36087441	missense	probably benign	0.00
R9728:Rgs22	UTSW	15	36040710	missense	probably benign	0.08
RF035:Rgs22	UTSW	15	36010835	critical splice acceptor site	probably benign

Predicted Primers

PCR Primer
(F):5'- GCTCGTTGCAGACACCTTAC -3'
(R):5'- CACAGTTAGAAAGATCGAGATGTGTAC -3'

Sequencing Primer
(F):5'- TACCTTACTGTGCGGTAGACAGC -3'
(R):5'- GCCCAGGTTAGCCTAAAAGTTATGC -3'

Posted On

2019-12-04