Incidental Mutation 'RF044:Krtap28-10'
ID 604945
Institutional Source Beutler Lab
Gene Symbol Krtap28-10
Ensembl Gene ENSMUSG00000100190
Gene Name keratin associated protein 28-10
Synonyms 4733401N17Rik
Accession Numbers
Essential gene? Probably non essential (E-score: 0.144) question?
Stock # RF044 (G1)
Quality Score 217.002
Status Not validated
Chromosome 1
Chromosomal Location 83019245-83020201 bp(-) (GRCm39)
Type of Mutation unclassified
DNA Base Change (assembly) CCACAG to CCACAGACACAG at 83019852 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000152431 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000045560] [ENSMUST00000164473] [ENSMUST00000188323] [ENSMUST00000222567]
AlphaFold A0A1Y7VP58
Predicted Effect probably benign
Transcript: ENSMUST00000045560
SMART Domains Protein: ENSMUSP00000041683
Gene: ENSMUSG00000038496

DomainStartEndE-ValueType
Pfam:Folate_carrier 11 435 1.4e-178 PFAM
Pfam:MFS_1 16 416 1.6e-17 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000164473
SMART Domains Protein: ENSMUSP00000126646
Gene: ENSMUSG00000038496

DomainStartEndE-ValueType
Pfam:Folate_carrier 11 435 1.3e-178 PFAM
Pfam:MFS_1 16 416 1.9e-17 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000188323
Predicted Effect probably benign
Transcript: ENSMUST00000222567
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.7%
  • 10x: 99.3%
  • 20x: 98.4%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 40 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A030005L19Rik TG TGTGGCTGCCG 1: 82,891,310 (GRCm39) probably benign Het
Blm CTCC CTCCTCCTCCTCATCC 7: 80,162,678 (GRCm39) probably benign Het
Cacna1f GAG GAGTAG X: 7,486,296 (GRCm39) probably null Het
Ccdc85c GCC GCCACC 12: 108,240,871 (GRCm39) probably benign Het
Chga AGC AGCGGC 12: 102,527,655 (GRCm39) probably benign Het
Crtam TTCTTGATCTGAA T 9: 40,895,650 (GRCm39) probably null Het
Dspp TGACAGCAGCGACAGCAG TGACAGCAGCGACAGCAGAGACAGCAGCGACAGCAG 5: 104,326,290 (GRCm39) probably benign Het
Efhd2 CCGCCG CCGCCGACGCCG 4: 141,602,079 (GRCm39) probably benign Het
F830016B08Rik AAAAAA AAAAAAAAA 18: 60,433,010 (GRCm39) probably benign Het
Gab3 TCT TCTCCT X: 74,043,611 (GRCm39) probably benign Het
Gabre CTC CTCCGGATC X: 71,313,667 (GRCm39) probably benign Het
Gykl1 G A 18: 52,827,488 (GRCm39) R232Q probably benign Het
Haus4 AGGGCAGGCCGCTGGCCACACACCTGGGAGGGGGGCAGGCCGCTGGCCACACACCTGGGAGGGGGGCAGGCCGCTGGCCACACACCTGGGAG AGGGCAGGCCGCTGGCCACACACCTGGGAGGGGGGCAGGCCGCTGGCCACACACCTGGGAG 14: 54,781,577 (GRCm39) probably null Het
Igf1r GATGGAGC GATGGAGCTGGATATGGAGC 7: 67,875,927 (GRCm39) probably benign Het
Irag2 TG TGAGCACATCG 6: 145,119,516 (GRCm39) probably benign Het
Maml2 ACAGCAGCAGCAACAGCAGCAGCAGCAGCA ACAGCAACAGCAGCAGCAGCAGCA 9: 13,532,752 (GRCm39) probably benign Het
Map1a CAGCTCCAGCTCCAGCTCCAGCTCCA CAGCTCCAGCTCCAGCTCCAGCTCCAGCTCAAGCTCCAGCTCCAGCTCCAGCTCCA 2: 121,136,774 (GRCm39) probably benign Het
Mapk6 CCAC CCACCTCAC 9: 75,295,542 (GRCm39) probably null Het
Morn4 GTGAG GTGAGTCAAGCATTGAG 19: 42,064,553 (GRCm39) probably null Het
Nefh CCTCACCTGGGGACTTGGCCTCACCTGGGGACTTGG CCTCACCTGGGGACTTGGACTCACCTGGGGACTTGGCCTCACCTGGGGACTTGG 11: 4,891,021 (GRCm39) probably benign Het
Nefh CTTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGA CTTGGCCTCACCTGGGGAATTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGA 11: 4,891,016 (GRCm39) probably benign Het
Nefh TCACCTGGGGACTTGGCCTCACCTGGGGACTTGGCCTC TCACCTGGGGACTTGGCCTCACCTGGGGACTTGGCCTCACCTGGGGACTTGGCCTC 11: 4,891,023 (GRCm39) probably benign Het
Nolc1 CAGCAGC CAGCAGCAGAAGCAGC 19: 46,069,810 (GRCm39) probably benign Het
Or10n7-ps1 AAAATA AAAATAAATA 9: 39,598,043 (GRCm39) probably null Het
Phc1 TG TGCTGCGG 6: 122,300,559 (GRCm39) probably benign Het
Pla2g4e AGGG A 2: 120,075,205 (GRCm39) probably benign Het
Prr36 TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC 8: 4,266,273 (GRCm39) probably benign Het
Ptdss1 G T 13: 67,093,412 (GRCm39) S84I possibly damaging Het
Ren1 ACCGC AC 1: 133,278,519 (GRCm39) probably benign Het
Rinl G T 7: 28,496,988 (GRCm39) G496V probably damaging Het
Ryr3 CTGA C 2: 112,740,869 (GRCm39) probably benign Het
Sbp A AAAAAGATGATGACAC 17: 24,164,340 (GRCm39) probably benign Het
Slc39a4 GTC GTCATCATGATCACCATGGTCACCATGATCACTGTGTTC 15: 76,499,070 (GRCm39) probably benign Het
Spaca1 TCTCGC TCTCGCGCTCGC 4: 34,049,846 (GRCm39) probably benign Het
Spaca1 TCGC TCGCTCGCGC 4: 34,049,854 (GRCm39) probably benign Het
Spmap2l ATCCTCCCCAGTCCCGCAAGGCCAG ATCCTCCCCAGTCCCGCAAGGCCAGCGCTCCTCCCCAGTCCCGCAAGGCCAG 5: 77,164,252 (GRCm39) probably benign Het
Strn C CCGTGCTCCCTTACCCCAGTCCGTGCTCCCTTACCCCAGTA 17: 78,984,717 (GRCm39) probably null Het
Tmem59 T TGTTTGTTG 4: 107,047,729 (GRCm39) probably benign Het
Tob1 GCA GCAACA 11: 94,105,287 (GRCm39) probably benign Het
Zfp683 TGTGG TGTGGTGG 4: 133,786,185 (GRCm39) probably benign Het
Other mutations in Krtap28-10
AlleleSourceChrCoordTypePredicted EffectPPH Score
FR4737:Krtap28-10 UTSW 1 83,019,844 (GRCm39) unclassified probably benign
R8865:Krtap28-10 UTSW 1 83,019,808 (GRCm39) missense unknown
R8984:Krtap28-10 UTSW 1 83,019,894 (GRCm39) missense unknown
RF001:Krtap28-10 UTSW 1 83,020,003 (GRCm39) unclassified probably benign
RF001:Krtap28-10 UTSW 1 83,019,976 (GRCm39) unclassified probably benign
RF001:Krtap28-10 UTSW 1 83,020,001 (GRCm39) unclassified probably benign
RF008:Krtap28-10 UTSW 1 83,019,974 (GRCm39) unclassified probably benign
RF008:Krtap28-10 UTSW 1 83,020,000 (GRCm39) unclassified probably benign
RF008:Krtap28-10 UTSW 1 83,019,849 (GRCm39) unclassified probably benign
RF008:Krtap28-10 UTSW 1 83,019,856 (GRCm39) unclassified probably benign
RF012:Krtap28-10 UTSW 1 83,019,857 (GRCm39) unclassified probably benign
RF013:Krtap28-10 UTSW 1 83,019,995 (GRCm39) unclassified probably benign
RF013:Krtap28-10 UTSW 1 83,019,856 (GRCm39) unclassified probably benign
RF014:Krtap28-10 UTSW 1 83,019,972 (GRCm39) unclassified probably benign
RF016:Krtap28-10 UTSW 1 83,019,844 (GRCm39) unclassified probably benign
RF017:Krtap28-10 UTSW 1 83,019,987 (GRCm39) unclassified probably benign
RF017:Krtap28-10 UTSW 1 83,019,859 (GRCm39) unclassified probably benign
RF018:Krtap28-10 UTSW 1 83,019,974 (GRCm39) unclassified probably benign
RF019:Krtap28-10 UTSW 1 83,019,990 (GRCm39) unclassified probably benign
RF023:Krtap28-10 UTSW 1 83,020,007 (GRCm39) unclassified probably benign
RF023:Krtap28-10 UTSW 1 83,019,867 (GRCm39) nonsense probably null
RF024:Krtap28-10 UTSW 1 83,019,973 (GRCm39) unclassified probably benign
RF024:Krtap28-10 UTSW 1 83,019,844 (GRCm39) unclassified probably benign
RF025:Krtap28-10 UTSW 1 83,019,979 (GRCm39) unclassified probably benign
RF026:Krtap28-10 UTSW 1 83,019,847 (GRCm39) unclassified probably benign
RF027:Krtap28-10 UTSW 1 83,020,006 (GRCm39) unclassified probably benign
RF028:Krtap28-10 UTSW 1 83,019,979 (GRCm39) unclassified probably benign
RF029:Krtap28-10 UTSW 1 83,019,991 (GRCm39) unclassified probably benign
RF032:Krtap28-10 UTSW 1 83,019,979 (GRCm39) unclassified probably benign
RF034:Krtap28-10 UTSW 1 83,020,003 (GRCm39) unclassified probably benign
RF035:Krtap28-10 UTSW 1 83,020,002 (GRCm39) unclassified probably benign
RF035:Krtap28-10 UTSW 1 83,019,867 (GRCm39) unclassified probably benign
RF037:Krtap28-10 UTSW 1 83,019,866 (GRCm39) unclassified probably benign
RF037:Krtap28-10 UTSW 1 83,020,007 (GRCm39) unclassified probably benign
RF038:Krtap28-10 UTSW 1 83,019,978 (GRCm39) unclassified probably benign
RF038:Krtap28-10 UTSW 1 83,019,849 (GRCm39) unclassified probably benign
RF042:Krtap28-10 UTSW 1 83,019,846 (GRCm39) unclassified probably benign
RF045:Krtap28-10 UTSW 1 83,019,982 (GRCm39) unclassified probably benign
RF045:Krtap28-10 UTSW 1 83,019,864 (GRCm39) unclassified probably benign
RF049:Krtap28-10 UTSW 1 83,020,006 (GRCm39) unclassified probably benign
RF049:Krtap28-10 UTSW 1 83,019,859 (GRCm39) unclassified probably benign
RF053:Krtap28-10 UTSW 1 83,019,999 (GRCm39) unclassified probably benign
RF055:Krtap28-10 UTSW 1 83,019,991 (GRCm39) unclassified probably benign
RF055:Krtap28-10 UTSW 1 83,019,983 (GRCm39) unclassified probably benign
RF055:Krtap28-10 UTSW 1 83,019,851 (GRCm39) unclassified probably benign
RF058:Krtap28-10 UTSW 1 83,019,983 (GRCm39) unclassified probably benign
RF059:Krtap28-10 UTSW 1 83,020,011 (GRCm39) unclassified probably benign
RF059:Krtap28-10 UTSW 1 83,019,996 (GRCm39) unclassified probably benign
RF061:Krtap28-10 UTSW 1 83,020,002 (GRCm39) unclassified probably benign
RF064:Krtap28-10 UTSW 1 83,019,852 (GRCm39) unclassified probably benign
Z1177:Krtap28-10 UTSW 1 83,019,880 (GRCm39) missense unknown
Predicted Primers PCR Primer
(F):5'- AGCATTTCCAGTGGGTAGATG -3'
(R):5'- TCCTCTCTACTGACAACATGGG -3'

Sequencing Primer
(F):5'- CATTTCCAGTGGGTAGATGAAAAG -3'
(R):5'- CTGTGGAGGCTGCGGAG -3'
Posted On 2019-12-04