Mutagenetix > Incidental Mutations

Incidental Mutation 'RF048:Znrd1as'

605086

Institutional Source

Beutler Lab

Gene Symbol

Znrd1as

Ensembl Gene

ENSMUSG00000036214

Gene Name

zinc ribbon domain containing 1, antisense

Synonyms

1700022C21Rik

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.163) question?

Stock #

RF048 (G1)

Quality Score

112.467

Status

Not validated

Chromosome

Chromosomal Location

36958592-36965625 bp(+) (GRCm38)

Type of Mutation

small insertion (7 aa in frame mutation)

DNA Base Change (assembly)

CCACCACCACCAC to CCACCACCACCACCCCCACCCACACCACCACCAC at 36965059 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000147556 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040177] [ENSMUST00000173814] [ENSMUST00000209623]

Predicted Effect

probably benign
Transcript: ENSMUST00000040177

SMART Domains

Protein: ENSMUSP00000048695
Gene: ENSMUSG00000036214

Domain	Start	End	E-Value	Type
low complexity region	98	115	N/A	INTRINSIC
coiled coil region	163	195	N/A	INTRINSIC
low complexity region	224	236	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000173814

SMART Domains

Protein: ENSMUSP00000134016
Gene: ENSMUSG00000036214

Domain	Start	End	E-Value	Type
low complexity region	19	36	N/A	INTRINSIC
coiled coil region	84	116	N/A	INTRINSIC
low complexity region	145	157	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000209623

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.3%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 22 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402H24Rik	CATC	CATCATC	2: 130,770,734		probably null	Het
Akna	GAGCTGA	G	4: 63,377,841		probably benign	Het
Celf4	G	T	18: 25,501,321	P326T	probably benign	Het
Chga	GCA	GCACCA	12: 102,561,403		probably benign	Het
Chga	GCA	GCATCA	12: 102,561,421		probably benign	Het
Cntnap1	TTT	TTTTGTT	11: 101,180,305		probably benign	Het
Cntnap1	AACCCC	AACCCCCACCCC	11: 101,189,563		probably benign	Het
Dnmt1	AGCACAGTTCCTACCTCGTT	AGCACAGTTCCTACCTCGTTTTGGGGGCGGGGCACAGTTCCTACCTCGTT	9: 20,910,126		probably null	Het
Gar1	CGCCGCCG	C	3: 129,830,700		probably null	Het
Gm15155	AA	AACAACAAA	X: 156,345,641		probably null	Het
Lrmp	GCACATTG	GCACATTGAACACATTG	6: 145,173,784		probably benign	Het
Mamld1	CA	CAGTA	X: 71,118,852		probably null	Het
Ncoa6	CTGTTG	CTG	2: 155,421,712		probably benign	Het
Nefh	CCTCAGCTGG	CCTCAGCTGGAGACTTGGGCTCAGCTGG	11: 4,941,003		probably benign	Het
Nefh	AGCTGGGGAC	AGCTGGGGACCTGGCCTCAGCTGGGGAC	11: 4,941,007		probably benign	Het
Nphs2	GCCACC	GCCACCCGAGGGGAGCCCCGAGCTCCAGCCTCCACC	1: 156,310,971		probably benign	Het
Nup155	T	TTTTTTG	15: 8,119,176		probably benign	Het
Nusap1	AGCAAGCTGAGA	AGCAAGCTGAGATACACGTTAGCATTGAGGCGCAAGCTGAGA	2: 119,627,599		probably benign	Het
Pdik1l	TGTTTT	TGTTTTGTTTTGGTTTT	4: 134,279,372		probably null	Het
Rnf144a	CTCTCTC	CTCTCTCTCTCTCTATCTCTC	12: 26,314,011		probably benign	Het
Sbp	G	GCTGACAACAAAGATT	17: 23,945,389		probably benign	Het
Strn	TC	TCCGTGCTCCCTTACCCCAGTGCGTGCGCCCTTACCCCAGAC	17: 78,677,287		probably null	Het

Other mutations in Znrd1as

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00807:Znrd1as	APN	17	36964921	missense	probably damaging	0.99
R0347:Znrd1as	UTSW	17	36965315	missense	probably damaging	1.00
R0789:Znrd1as	UTSW	17	36964960	missense	probably damaging	1.00
R0993:Znrd1as	UTSW	17	36965047	small deletion	probably benign
R2110:Znrd1as	UTSW	17	36965444	missense	possibly damaging	0.47
R2866:Znrd1as	UTSW	17	36965160	missense	possibly damaging	0.91
R4224:Znrd1as	UTSW	17	36958725	utr 5 prime	probably benign
R4746:Znrd1as	UTSW	17	36964873	missense	probably benign	0.00
R7449:Znrd1as	UTSW	17	36964383	missense	probably damaging	1.00
RF005:Znrd1as	UTSW	17	36965048	small insertion	probably benign
RF008:Znrd1as	UTSW	17	36965054	small insertion	probably benign
RF010:Znrd1as	UTSW	17	36965063	small insertion	probably benign
RF014:Znrd1as	UTSW	17	36965060	small insertion	probably benign
RF024:Znrd1as	UTSW	17	36965057	small insertion	probably benign
RF025:Znrd1as	UTSW	17	36965048	small insertion	probably benign
RF029:Znrd1as	UTSW	17	36965071	small insertion	probably benign
RF035:Znrd1as	UTSW	17	36965066	small insertion	probably benign
RF046:Znrd1as	UTSW	17	36965057	small insertion	probably benign
RF053:Znrd1as	UTSW	17	36965066	small insertion	probably benign
RF064:Znrd1as	UTSW	17	36965050	small insertion	probably benign

Predicted Primers

PCR Primer
(F):5'- GTGCATACAACAGCAGCAAG -3'
(R):5'- GTGGGATGCTTTCCTCCTCTAG -3'

Sequencing Primer
(F):5'- CTTGATAACTCAGAAATGCCATGAGG -3'
(R):5'- TCCTCTAGCACAATTTTGGGTAG -3'

Posted On

2019-12-04