Mutagenetix > Incidental Mutation

Incidental Mutation 'R0128:Vmn2r85'

60517

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r85

Ensembl Gene

ENSMUSG00000092048

Gene Name

vomeronasal 2, receptor 85

Synonyms

EG623734

MMRRC Submission

038413-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R0128 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130253658-130266615 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 130255054 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000128792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171811]

AlphaFold

G3UW56

Predicted Effect

probably benign
Transcript: ENSMUST00000171811

SMART Domains

Protein: ENSMUSP00000128792
Gene: ENSMUSG00000092048

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	9e-26	PFAM
Pfam:NCD3G	508	562	1.1e-18	PFAM
Pfam:7tm_3	595	831	3.7e-54	PFAM

Coding Region Coverage

1x: 99.0%
3x: 97.6%
10x: 93.0%
20x: 79.3%

Validation Efficiency

99% (84/85)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	G	6: 128,552,602 (GRCm39)		probably benign	Het
Abcd4	T	G	12: 84,659,126 (GRCm39)	Q210P	possibly damaging	Het
Ablim2	G	A	5: 35,966,520 (GRCm39)		probably benign	Het
Acte1	G	T	7: 143,445,492 (GRCm39)	G178C	probably damaging	Het
Actl6b	A	G	5: 137,553,327 (GRCm39)	N113S	probably benign	Het
Actn3	A	T	19: 4,921,643 (GRCm39)	V179E	probably damaging	Het
Aff4	C	A	11: 53,306,293 (GRCm39)	T1145N	probably damaging	Het
Ankrd42	G	A	7: 92,241,067 (GRCm39)	Q431*	probably null	Het
Anxa9	A	G	3: 95,209,733 (GRCm39)	S129P	probably benign	Het
Arfgef2	T	G	2: 166,677,639 (GRCm39)	I88S	probably damaging	Het
Asap3	C	A	4: 135,961,915 (GRCm39)	N285K	probably damaging	Het
Atp6v0a2	A	G	5: 124,790,248 (GRCm39)	N477S	probably damaging	Het
Atp7b	C	T	8: 22,518,188 (GRCm39)	E205K	possibly damaging	Het
Atp8b5	T	A	4: 43,369,715 (GRCm39)		probably null	Het
C87436	G	A	6: 86,446,809 (GRCm39)	G533D	probably damaging	Het
Ccdc138	T	A	10: 58,364,182 (GRCm39)	I314N	probably damaging	Het
Ccs	A	G	19: 4,875,654 (GRCm39)	F237S	probably damaging	Het
Ccz1	T	G	5: 143,946,112 (GRCm39)		probably benign	Het
Cdcp2	C	T	4: 106,963,904 (GRCm39)		probably benign	Het
Chd1	A	G	17: 17,613,829 (GRCm39)	N531S	probably damaging	Het
Clptm1	A	T	7: 19,368,932 (GRCm39)	F476I	probably damaging	Het
Colec12	C	T	18: 9,858,921 (GRCm39)	P568L	unknown	Het
Cped1	T	A	6: 22,121,038 (GRCm39)	Y373N	probably benign	Het
Cr2	A	T	1: 194,848,539 (GRCm39)	V328D	probably damaging	Het
D630045J12Rik	A	T	6: 38,126,706 (GRCm39)		probably benign	Het
Dcdc2a	A	T	13: 25,371,655 (GRCm39)		probably benign	Het
Dlg1	G	T	16: 31,676,883 (GRCm39)		probably null	Het
Epb41l5	A	C	1: 119,477,632 (GRCm39)	V705G	possibly damaging	Het
Ergic3	C	A	2: 155,853,060 (GRCm39)	R43S	possibly damaging	Het
Flnb	T	C	14: 7,901,951 (GRCm38)	V938A	probably damaging	Het
Frmd4a	T	C	2: 4,608,903 (GRCm39)	Y928H	probably damaging	Het
Fyn	C	T	10: 39,387,978 (GRCm39)	T78M	probably benign	Het
Gdap2	A	G	3: 100,109,311 (GRCm39)	T443A	probably damaging	Het
Ghrl	A	T	6: 113,694,129 (GRCm39)		probably benign	Het
Gm4787	T	A	12: 81,424,521 (GRCm39)	K546*	probably null	Het
Gm6576	C	G	15: 27,026,086 (GRCm39)		noncoding transcript	Het
Got1	C	T	19: 43,512,816 (GRCm39)	D27N	probably benign	Het
Gucy2c	C	T	6: 136,681,247 (GRCm39)	V946I	probably damaging	Het
Hectd4	T	C	5: 121,487,306 (GRCm39)	Y3434H	possibly damaging	Het
Hp1bp3	C	T	4: 137,964,520 (GRCm39)	S348F	probably damaging	Het
Itpr1	A	G	6: 108,448,170 (GRCm39)		probably benign	Het
Kctd1	G	A	18: 15,107,237 (GRCm39)	P743S	probably benign	Het
Klhl23	T	C	2: 69,664,310 (GRCm39)	V553A	probably damaging	Het
Krt24	T	C	11: 99,171,093 (GRCm39)	D495G	probably damaging	Het
L3hypdh	C	T	12: 72,123,917 (GRCm39)		probably null	Het
Lipo3	C	T	19: 33,534,506 (GRCm39)		probably null	Het
Lman2l	G	T	1: 36,463,945 (GRCm39)	S171*	probably null	Het
Lrp1b	T	C	2: 41,401,520 (GRCm39)	D378G	probably damaging	Het
Map3k4	T	A	17: 12,466,950 (GRCm39)	D1104V	probably damaging	Het
Mpeg1	T	C	19: 12,438,587 (GRCm39)	V15A	probably benign	Het
Narf	C	T	11: 121,141,662 (GRCm39)	R356C	probably damaging	Het
Nebl	T	A	2: 17,397,834 (GRCm39)	Q487H	possibly damaging	Het
Olfm5	G	A	7: 103,810,133 (GRCm39)	A76V	probably benign	Het
Or1j11	T	A	2: 36,312,299 (GRCm39)	D296E	probably benign	Het
Or2z8	C	T	8: 72,812,244 (GRCm39)	T240M	probably damaging	Het
Or52p1	A	T	7: 104,267,788 (GRCm39)	I301F	probably damaging	Het
Or5ak22	T	A	2: 85,230,305 (GRCm39)	S191C	probably damaging	Het
Or8k40	T	C	2: 86,584,231 (GRCm39)	M284V	probably benign	Het
Palb2	A	T	7: 121,727,389 (GRCm39)	Y160*	probably null	Het
Pasd1	T	C	X: 70,983,161 (GRCm39)	C378R	possibly damaging	Het
Paxip1	C	T	5: 27,949,183 (GRCm39)		probably benign	Het
Pclo	A	G	5: 14,729,811 (GRCm39)		probably benign	Het
Pdcd11	G	A	19: 47,108,301 (GRCm39)	V1223I	probably benign	Het
Pde6c	T	C	19: 38,157,813 (GRCm39)		probably benign	Het
Prr12	A	G	7: 44,699,463 (GRCm39)		probably benign	Het
Prss39	T	A	1: 34,541,281 (GRCm39)		probably benign	Het
Samd5	A	G	10: 9,550,683 (GRCm39)	W9R	probably damaging	Het
Sfr1	A	G	19: 47,723,457 (GRCm39)	*320W	probably null	Het
Sft2d1rt	A	G	11: 45,943,120 (GRCm39)	M1T	probably null	Het
Sh3bp4	A	G	1: 89,073,036 (GRCm39)	N628S	possibly damaging	Het
Sim1	A	T	10: 50,784,057 (GRCm39)	I104F	probably damaging	Het
Slc1a3	T	C	15: 8,665,693 (GRCm39)	M519V	probably benign	Het
Smcp	T	A	3: 92,491,827 (GRCm39)	T7S	unknown	Het
Sp4	A	G	12: 118,264,551 (GRCm39)		probably benign	Het
Spag9	T	A	11: 93,984,365 (GRCm39)	I327N	probably damaging	Het
Thbs4	G	T	13: 92,890,918 (GRCm39)	H850N	probably benign	Het
Ubap2l	A	T	3: 89,928,680 (GRCm39)	S478T	possibly damaging	Het
Unc79	A	G	12: 103,054,693 (GRCm39)		probably benign	Het
Wrap73	A	G	4: 154,226,957 (GRCm39)	D19G	possibly damaging	Het

Other mutations in Vmn2r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01298:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01361:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL02185:Vmn2r85	APN	10	130,254,561 (GRCm39)	missense	probably benign	0.13
IGL02505:Vmn2r85	APN	10	130,261,449 (GRCm39)	missense	probably damaging	1.00
IGL02607:Vmn2r85	APN	10	130,262,290 (GRCm39)	missense	possibly damaging	0.89
IGL02755:Vmn2r85	APN	10	130,261,381 (GRCm39)	missense	probably damaging	0.98
IGL03188:Vmn2r85	APN	10	130,254,612 (GRCm39)	missense	probably benign	0.16
IGL03366:Vmn2r85	APN	10	130,262,328 (GRCm39)	missense	probably benign	0.00
IGL03397:Vmn2r85	APN	10	130,261,263 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r85	UTSW	10	130,261,572 (GRCm39)	missense	probably benign	0.00
R0066:Vmn2r85	UTSW	10	130,261,770 (GRCm39)	missense	probably damaging	1.00
R0130:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0503:Vmn2r85	UTSW	10	130,258,609 (GRCm39)	missense	probably damaging	1.00
R0827:Vmn2r85	UTSW	10	130,265,387 (GRCm39)	missense	possibly damaging	0.89
R1432:Vmn2r85	UTSW	10	130,261,155 (GRCm39)	missense	possibly damaging	0.74
R1521:Vmn2r85	UTSW	10	130,261,788 (GRCm39)	missense	probably damaging	0.99
R2029:Vmn2r85	UTSW	10	130,261,443 (GRCm39)	nonsense	probably null
R2034:Vmn2r85	UTSW	10	130,262,242 (GRCm39)	splice site	probably benign
R2852:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R2853:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R3084:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3085:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3430:Vmn2r85	UTSW	10	130,254,758 (GRCm39)	missense	probably damaging	0.97
R3694:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R3932:Vmn2r85	UTSW	10	130,254,336 (GRCm39)	missense	probably damaging	1.00
R4207:Vmn2r85	UTSW	10	130,254,574 (GRCm39)	missense	probably damaging	1.00
R4628:Vmn2r85	UTSW	10	130,261,235 (GRCm39)	missense	probably benign	0.00
R4814:Vmn2r85	UTSW	10	130,254,567 (GRCm39)	missense	probably benign	0.12
R4948:Vmn2r85	UTSW	10	130,254,990 (GRCm39)	missense	probably damaging	1.00
R4951:Vmn2r85	UTSW	10	130,261,113 (GRCm39)	missense	probably damaging	1.00
R4959:Vmn2r85	UTSW	10	130,257,302 (GRCm39)	missense	probably damaging	1.00
R5336:Vmn2r85	UTSW	10	130,258,574 (GRCm39)	missense	possibly damaging	0.63
R5643:Vmn2r85	UTSW	10	130,262,343 (GRCm39)	missense	probably damaging	1.00
R6061:Vmn2r85	UTSW	10	130,261,531 (GRCm39)	missense	probably benign	0.09
R6115:Vmn2r85	UTSW	10	130,258,672 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r85	UTSW	10	130,261,330 (GRCm39)	missense	possibly damaging	0.88
R6518:Vmn2r85	UTSW	10	130,265,281 (GRCm39)	missense	probably benign	0.00
R6533:Vmn2r85	UTSW	10	130,262,529 (GRCm39)	missense	probably benign	0.00
R6610:Vmn2r85	UTSW	10	130,261,838 (GRCm39)	missense	probably damaging	0.97
R6809:Vmn2r85	UTSW	10	130,261,795 (GRCm39)	missense	probably benign
R6962:Vmn2r85	UTSW	10	130,261,452 (GRCm39)	missense	probably damaging	0.99
R7075:Vmn2r85	UTSW	10	130,258,557 (GRCm39)	missense	probably benign	0.06
R7104:Vmn2r85	UTSW	10	130,262,376 (GRCm39)	missense	probably benign
R7424:Vmn2r85	UTSW	10	130,254,849 (GRCm39)	missense	probably damaging	1.00
R7516:Vmn2r85	UTSW	10	130,254,852 (GRCm39)	missense	probably damaging	1.00
R7537:Vmn2r85	UTSW	10	130,258,735 (GRCm39)	missense	probably benign	0.01
R7768:Vmn2r85	UTSW	10	130,254,562 (GRCm39)	missense	probably damaging	1.00
R7810:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R8078:Vmn2r85	UTSW	10	130,265,364 (GRCm39)	nonsense	probably null
R8115:Vmn2r85	UTSW	10	130,261,820 (GRCm39)	missense	probably benign	0.06
R8262:Vmn2r85	UTSW	10	130,254,738 (GRCm39)	missense	probably damaging	0.98
R8395:Vmn2r85	UTSW	10	130,261,797 (GRCm39)	missense	probably damaging	0.99
R8409:Vmn2r85	UTSW	10	130,261,257 (GRCm39)	missense	probably benign	0.16
R8547:Vmn2r85	UTSW	10	130,261,311 (GRCm39)	missense	probably damaging	1.00
R8875:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R9035:Vmn2r85	UTSW	10	130,261,479 (GRCm39)	missense	probably benign
R9040:Vmn2r85	UTSW	10	130,254,311 (GRCm39)	missense	probably damaging	1.00
R9115:Vmn2r85	UTSW	10	130,254,153 (GRCm39)	missense	probably benign	0.00
R9182:Vmn2r85	UTSW	10	130,265,350 (GRCm39)	missense	probably benign	0.00
R9245:Vmn2r85	UTSW	10	130,261,534 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r85	UTSW	10	130,255,033 (GRCm39)	missense	possibly damaging	0.92
R9405:Vmn2r85	UTSW	10	130,261,215 (GRCm39)	missense	probably damaging	0.99
R9502:Vmn2r85	UTSW	10	130,261,387 (GRCm39)	missense	probably damaging	0.99
R9520:Vmn2r85	UTSW	10	130,254,993 (GRCm39)	missense	probably benign
R9653:Vmn2r85	UTSW	10	130,261,694 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r85	UTSW	10	130,261,713 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r85	UTSW	10	130,254,776 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGAGCTTGAAAGCCATGACCAC -3'
(R):5'- TGGTGATCTGTTCCCTCACAATGC -3'

Sequencing Primer
(F):5'- GGCCTTCACAATGGGAGTATC -3'
(R):5'- TGTTCCCTCACAATGCATAGG -3'

Posted On

2013-07-24