Mutagenetix > Incidental Mutation

Incidental Mutation 'RF053:Krtap28-10'

605176

Institutional Source

Beutler Lab

Gene Symbol

Krtap28-10

Ensembl Gene

ENSMUSG00000100190

Gene Name

keratin associated protein 28-10

Synonyms

4733401N17Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.144) question?

Stock #

RF053 (G1)

Quality Score

217.471

Status

Not validated

Chromosome

Chromosomal Location

83019245-83020201 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

CAGCCACCACAGC to CAGCCACCACAGCCAAAGCCACCACAGC at 83019999 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152431 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045560] [ENSMUST00000164473] [ENSMUST00000188323] [ENSMUST00000222567]

AlphaFold

A0A1Y7VP58

Predicted Effect

probably benign
Transcript: ENSMUST00000045560

SMART Domains

Protein: ENSMUSP00000041683
Gene: ENSMUSG00000038496

Domain	Start	End	E-Value	Type
Pfam:Folate_carrier	11	435	1.4e-178	PFAM
Pfam:MFS_1	16	416	1.6e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000164473

SMART Domains

Protein: ENSMUSP00000126646
Gene: ENSMUSG00000038496

Domain	Start	End	E-Value	Type
Pfam:Folate_carrier	11	435	1.3e-178	PFAM
Pfam:MFS_1	16	416	1.9e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188323

Predicted Effect

probably benign
Transcript: ENSMUST00000222567

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.3%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 20 list
Gene	Ref	Var	Chr/Loc	Predicted Effect	Zygosity
A030005L19Rik	TGGCTGCTG	TGGCTGCTGGGGCTGCTG	1: 82,891,294 (GRCm39)	probably benign	Het
Abra	TGGC	T	15: 41,729,695 (GRCm39)	probably benign	Het
Blm	CTCCTCCTCCTC	CTCCTCCTCCTCATCCTCCTCCTC	7: 80,162,669 (GRCm39)	probably benign	Het
Bmp5	TGAGGAG	T	9: 75,683,656 (GRCm39)	probably benign	Het
Cngb1	GGCTCTGGCTCTGGCTCTGGCTCTG	GG	8: 96,030,276 (GRCm39)	probably null	Het
Cyb5r4	GGATGTGACAGACACACTGCCCAG	GGATGTGACAGACACACTGCCCAGCGATGTGACAGACACACTGCCCAG	9: 86,922,475 (GRCm39)	probably benign	Het
Ehbp1l1	TCACACCACC	T	19: 5,766,030 (GRCm39)	probably benign	Het
Kdm3a	TTTTT	TTTTTT	6: 71,609,033 (GRCm39)	probably benign	Het
Mamld1	CA	CAGAA	X: 70,162,458 (GRCm39)	probably benign	Het
Map1a	CTCCAGCTCCAGCTCCAGCTCCAGCTCCA	CTCCAGCTCCAGCTCCAGCTCCAGCTCCAGATCCAGCTCCAGCTCCAGCTCCAGCTCCA	2: 121,136,771 (GRCm39)	probably benign	Het
Med12l	CAG	CAGAAG	3: 59,183,414 (GRCm39)	probably benign	Het
Nefh	GACTTGGCCTCACCT	GACTTGGCCTCACCTCACCACTTGGCCTCACCT	11: 4,891,014 (GRCm39)	probably null	Het
Polr1has	CACCAC	CACCACCACCACCACCACCTCTACCAC	17: 37,275,958 (GRCm39)	probably benign	Het
Rap1gds1	TCATTTATTATGACCATAC	TC	3: 138,647,418 (GRCm39)	probably null	Het
Tcof1	C	CAGA	18: 60,968,819 (GRCm39)	probably benign	Het
Tfeb	AGC	AGCCGC	17: 48,097,039 (GRCm39)	probably benign	Het
Trappc9	TGCT	TGCTGCTGCTGCTGCGGCT	15: 72,673,177 (GRCm39)	probably benign	Het
Usp2	C	CTCATGTGACCTGTTCTTCACTTAA	9: 44,000,426 (GRCm39)	probably benign	Het
Zfp598	CCCACCACCACAACCACCACCACCACCACCAC	CCCACCACCACCACCACAACCACCACCACCACCACCAC	17: 24,899,735 (GRCm39)	probably benign	Het
Zfp69	GTGCCAGGGGTCTCTCCATCATGGGATCCTGCTGCAGGGATGCCAGGGGTCTCTCCATCATGGGATCCTGCTGCAGGGGTGCCAGGGGTCGCTTCATCATGGGATCCTGTTGCAGTGGTGCCAGGGGTCGCTCCATCATGGGATCCTGCTGCAGGGGTGCCAGGGGTC	GTGCCAGGGGTCTCTCCATCATGGGATCCTGCTGCAGGGGTGCCAGGGGTCGCTTCATCATGGGATCCTGTTGCAGTGGTGCCAGGGGTCGCTCCATCATGGGATCCTGCTGCAGGGGTGCCAGGGGTC	4: 120,804,544 (GRCm39)	probably benign	Het

Other mutations in Krtap28-10

Allele	Source	Chr	Coord	Type	Predicted Effect
FR4737:Krtap28-10	UTSW	1	83,019,844 (GRCm39)	unclassified	probably benign
R8865:Krtap28-10	UTSW	1	83,019,808 (GRCm39)	missense	unknown
R8984:Krtap28-10	UTSW	1	83,019,894 (GRCm39)	missense	unknown
RF001:Krtap28-10	UTSW	1	83,020,003 (GRCm39)	unclassified	probably benign
RF001:Krtap28-10	UTSW	1	83,019,976 (GRCm39)	unclassified	probably benign
RF001:Krtap28-10	UTSW	1	83,020,001 (GRCm39)	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83,019,974 (GRCm39)	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83,020,000 (GRCm39)	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83,019,849 (GRCm39)	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83,019,856 (GRCm39)	unclassified	probably benign
RF012:Krtap28-10	UTSW	1	83,019,857 (GRCm39)	unclassified	probably benign
RF013:Krtap28-10	UTSW	1	83,019,995 (GRCm39)	unclassified	probably benign
RF013:Krtap28-10	UTSW	1	83,019,856 (GRCm39)	unclassified	probably benign
RF014:Krtap28-10	UTSW	1	83,019,972 (GRCm39)	unclassified	probably benign
RF016:Krtap28-10	UTSW	1	83,019,844 (GRCm39)	unclassified	probably benign
RF017:Krtap28-10	UTSW	1	83,019,987 (GRCm39)	unclassified	probably benign
RF017:Krtap28-10	UTSW	1	83,019,859 (GRCm39)	unclassified	probably benign
RF018:Krtap28-10	UTSW	1	83,019,974 (GRCm39)	unclassified	probably benign
RF019:Krtap28-10	UTSW	1	83,019,990 (GRCm39)	unclassified	probably benign
RF023:Krtap28-10	UTSW	1	83,020,007 (GRCm39)	unclassified	probably benign
RF023:Krtap28-10	UTSW	1	83,019,867 (GRCm39)	nonsense	probably null
RF024:Krtap28-10	UTSW	1	83,019,973 (GRCm39)	unclassified	probably benign
RF024:Krtap28-10	UTSW	1	83,019,844 (GRCm39)	unclassified	probably benign
RF025:Krtap28-10	UTSW	1	83,019,979 (GRCm39)	unclassified	probably benign
RF026:Krtap28-10	UTSW	1	83,019,847 (GRCm39)	unclassified	probably benign
RF027:Krtap28-10	UTSW	1	83,020,006 (GRCm39)	unclassified	probably benign
RF028:Krtap28-10	UTSW	1	83,019,979 (GRCm39)	unclassified	probably benign
RF029:Krtap28-10	UTSW	1	83,019,991 (GRCm39)	unclassified	probably benign
RF032:Krtap28-10	UTSW	1	83,019,979 (GRCm39)	unclassified	probably benign
RF034:Krtap28-10	UTSW	1	83,020,003 (GRCm39)	unclassified	probably benign
RF035:Krtap28-10	UTSW	1	83,020,002 (GRCm39)	unclassified	probably benign
RF035:Krtap28-10	UTSW	1	83,019,867 (GRCm39)	unclassified	probably benign
RF037:Krtap28-10	UTSW	1	83,019,866 (GRCm39)	unclassified	probably benign
RF037:Krtap28-10	UTSW	1	83,020,007 (GRCm39)	unclassified	probably benign
RF038:Krtap28-10	UTSW	1	83,019,978 (GRCm39)	unclassified	probably benign
RF038:Krtap28-10	UTSW	1	83,019,849 (GRCm39)	unclassified	probably benign
RF042:Krtap28-10	UTSW	1	83,019,846 (GRCm39)	unclassified	probably benign
RF044:Krtap28-10	UTSW	1	83,019,852 (GRCm39)	unclassified	probably benign
RF045:Krtap28-10	UTSW	1	83,019,982 (GRCm39)	unclassified	probably benign
RF045:Krtap28-10	UTSW	1	83,019,864 (GRCm39)	unclassified	probably benign
RF049:Krtap28-10	UTSW	1	83,020,006 (GRCm39)	unclassified	probably benign
RF049:Krtap28-10	UTSW	1	83,019,859 (GRCm39)	unclassified	probably benign
RF055:Krtap28-10	UTSW	1	83,019,991 (GRCm39)	unclassified	probably benign
RF055:Krtap28-10	UTSW	1	83,019,983 (GRCm39)	unclassified	probably benign
RF055:Krtap28-10	UTSW	1	83,019,851 (GRCm39)	unclassified	probably benign
RF058:Krtap28-10	UTSW	1	83,019,983 (GRCm39)	unclassified	probably benign
RF059:Krtap28-10	UTSW	1	83,020,011 (GRCm39)	unclassified	probably benign
RF059:Krtap28-10	UTSW	1	83,019,996 (GRCm39)	unclassified	probably benign
RF061:Krtap28-10	UTSW	1	83,020,002 (GRCm39)	unclassified	probably benign
RF064:Krtap28-10	UTSW	1	83,019,852 (GRCm39)	unclassified	probably benign
Z1177:Krtap28-10	UTSW	1	83,019,880 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GCTAGCAGCAGCACTTCTTG -3'
(R):5'- CTCCTTGAAGCAAAGGCCATC -3'

Sequencing Primer
(F):5'- TGGCAGCAGCACTTCTG -3'
(R):5'- TTGAAGCAAAGGCCATCACATCTC -3'

Posted On

2019-12-04