Mutagenetix > Incidental Mutation

Incidental Mutation 'RF057:6030445D17Rik'

605297

Institutional Source

Beutler Lab

Gene Symbol

6030445D17Rik

Ensembl Gene

ENSMUSG00000066178

Gene Name

RIKEN cDNA 6030445D17 gene

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

RF057 (G1)

Quality Score

135.593

Status

Not validated

Chromosome

Chromosomal Location

136189561-136191288 bp(+) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

GCACATGCACACACA to GCACA at 136189617 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000081641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084593]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000084593

SMART Domains

Protein: ENSMUSP00000081641
Gene: ENSMUSG00000066178

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC

Coding Region Coverage

1x: 99.8%
3x: 99.6%
10x: 99.3%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 26 list
Gene	Ref	Var	Chr/Loc	Predicted Effect	Zygosity
Alpk3	AAGGCACCGAC	A	7: 80,742,165 (GRCm39)	probably null	Het
Ankhd1	CGGC	CGGCGGTGGC	18: 36,693,982 (GRCm39)	probably benign	Het
Calhm1	GCTGTGGC	GCTGTGGCTGTGTCTGTGGC	19: 47,129,709 (GRCm39)	probably benign	Het
Crtam	TTCTTGATCTGAA	T	9: 40,895,650 (GRCm39)	probably null	Het
Cul9	CCT	CCTACT	17: 46,811,789 (GRCm39)	probably null	Het
Dmkn	GAAGTGGTGGAAGTGGTGG	GAAGTGGTGGAAGTGGTGGAAGTGGTGTAAGTGGTGGAAGTGGTGG	7: 30,466,613 (GRCm39)	probably benign	Het
Efhd2	CGCCG	CGCCGAAGCCG	4: 141,602,080 (GRCm39)	probably benign	Het
Eps8	CGCTC	CGCTCGCTC	6: 137,494,062 (GRCm39)	probably benign	Het
Garin5a	GGAGGA	GGAGGAAGGCTGGATCCTGGATACCTGGGTCTGAGTGAGGA	7: 44,149,956 (GRCm39)	probably null	Het
Gm16519	AAGCAG	AAGCAGCAG	17: 71,236,326 (GRCm39)	probably benign	Het
Krt10	CGCC	CGCCGCC	11: 99,277,025 (GRCm39)	probably benign	Het
Lypd8	CCAACA	CCAACAGTTCCCTCGCCTCTGTTACCCCACAAATCAACAACA	11: 58,281,065 (GRCm39)	probably benign	Het
Mapk6	CCCCA	CCCCATCCCA	9: 75,295,540 (GRCm39)	probably null	Het
Med12l	GCA	GCATCA	3: 59,183,401 (GRCm39)	probably benign	Het
Morn4	GTCAGGCAGTGA	GTCAGGCAGTGACTCAGGCAGTGA	19: 42,064,545 (GRCm39)	probably null	Het
Pdik1l	TTTTTGTTTT	TTTTTGTTTTGGTTTTGTTTT	4: 134,006,679 (GRCm39)	probably null	Het
Prp2	CACCACCACCAGGTGGCCCACAGCCGAGACCCCCTCAAGGCCCA	C	6: 132,577,493 (GRCm39)	probably null	Het
Rtbdn	GCAGCG	GCAGCGACAGCG	8: 85,682,795 (GRCm39)	probably benign	Het
Tcof1	TTTCCCAGAGATCCCCTTGGC	TTTCCCAGAGATCCCCTTGGCTGCTGAGATGGGCACCTTCCCAGAGATCCCCTTGGC	18: 60,966,636 (GRCm39)	probably benign	Het
Tcof1	GAGATCCCCTTGGC	GAGATCCCCTTGGCTGCTGAGATGGGCACTTTCCCATAGATCCCCTTGGC	18: 60,966,643 (GRCm39)	probably benign	Het
Tcof1	TCCCAGAGATCCCCTTGGC	TCCCAGAGATCCCCTTGGCTGCTGAGATGGGCACTTGCCCAGAGATCCCCTTGGC	18: 60,966,638 (GRCm39)	probably benign	Het
Tcof1	TTCCCAGAGATCCCCTTGGC	TTCCCAGAGATCCCCTTGGCTGCTGAGATGGGCACTGTCCCAGAGATCCCCTTGGC	18: 60,966,637 (GRCm39)	probably benign	Het
Tgoln1	CAGAAT	CAGAATCACCTCCCGTGGGCTTGCGAGAAT	6: 72,593,052 (GRCm39)	probably benign	Het
Trappc9	TGCTGCTGCTGCTGCTGCTGCTGCTGCTGC	TGCTGCTGCTGCTGCGGCTGCTGCTGCTGCTGCTGCTGCTGCTGC	15: 72,673,144 (GRCm39)	probably benign	Het
Ttn	ACCTCCTCCTCCTCCTCCTC	ACCTCCTCCTCCTCCTC	2: 76,673,734 (GRCm39)	probably benign	Het
Zfp384	C	CCCAGGCCCAGGG	6: 125,013,459 (GRCm39)	probably benign	Het

Other mutations in 6030445D17Rik

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R6219:6030445D17Rik	UTSW	4	136,190,059 (GRCm39)	missense	unknown
RF009:6030445D17Rik	UTSW	4	136,189,661 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- GACGACCTGAGTTAGAATAGACC -3'
(R):5'- TTTCCTGAGCCGGACGAC -3'

Sequencing Primer
(F):5'- AGACCCTATAAATTGTCCTCGG -3'
(R):5'- ACCGGGGGATGGGACTTG -3'

Posted On

2019-12-04