Mutagenetix > Incidental Mutation

Incidental Mutation 'RF058:Krtap28-10'

605321

Institutional Source

Beutler Lab

Gene Symbol

Krtap28-10

Ensembl Gene

ENSMUSG00000100190

Gene Name

keratin associated protein 28-10

Synonyms

4733401N17Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.142) question?

Stock #

RF058 (G1)

Quality Score

138.467

Status

Not validated

Chromosome

Chromosomal Location

83041524-83042480 bp(-) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

ACAGCCACCACAGCCACAGCCACCACAGC to ACAGCCACCACAGCCCCAGCCACCACAGCCACAGCCACCACAGC at 83042262 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152431 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045560] [ENSMUST00000164473] [ENSMUST00000188323] [ENSMUST00000222567]

AlphaFold

A0A1Y7VP58

Predicted Effect

probably benign
Transcript: ENSMUST00000045560

SMART Domains

Protein: ENSMUSP00000041683
Gene: ENSMUSG00000038496

Domain	Start	End	E-Value	Type
Pfam:Folate_carrier	11	435	1.4e-178	PFAM
Pfam:MFS_1	16	416	1.6e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000164473

SMART Domains

Protein: ENSMUSP00000126646
Gene: ENSMUSG00000038496

Domain	Start	End	E-Value	Type
Pfam:Folate_carrier	11	435	1.3e-178	PFAM
Pfam:MFS_1	16	416	1.9e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188323

Predicted Effect

probably benign
Transcript: ENSMUST00000222567

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.3%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 22 list
Gene	Ref	Var	Chr/Loc	Predicted Effect	Zygosity
3110021N24Rik	GAGCGCGGCC	G	4: 108,780,629	probably benign	Het
5430401F13Rik	AAGGAAAAGGTGGCCAG	AAGGAAAAGGTGGCCAGCAAAAACAGAGAGGAAAAGGTGGCCAG	6: 131,552,887	probably benign	Het
5430401F13Rik	CAG	CAGCAAAAACAGAAAGGAAAAGGTGGCCAG	6: 131,552,901	probably benign	Het
Alg9	GGC	GGCTGC	9: 50,775,427	probably benign	Het
Arid1b	CGGGGG	CGGGGGGGG	17: 4,995,583	probably benign	Het
Chd4	GC	GCTCCCTC	6: 125,122,131	probably benign	Het
Chga	CAG	CAGAAG	12: 102,561,416	probably benign	Het
Cort	GCCCACTCGT	G	4: 149,125,412	probably benign	Het
Flywch1	GT	GTGGGGAGGCTACGTGCTCACCCGCTCCTGGTAT	17: 23,762,177	probably null	Het
Gab3	TCT	TCTACT	X: 75,000,002	probably benign	Het
Gabre	C	CCGGCTG	X: 72,270,063	probably benign	Het
Haus4	CACTTAAAAAAAAAA	CA	14: 54,550,035	probably benign	Het
Il2	AG	AGGGCTTGAAGTGG	3: 37,125,817	probably benign	Het
Il2	CTTGAAGTGG	CTTGAAGTGGGGATTGAAGTGG	3: 37,125,821	probably benign	Het
Kri1	TCCTCCTCC	TC	9: 21,281,066	probably null	Het
Mbd1	CGTCTTCGTCTGCATCTGCATCTGCA	C	18: 74,273,609	probably null	Het
Nefh	CCTC	CCTCGCCTGGGGACTTGGACTC	11: 4,941,021	probably benign	Het
Rtbdn	GCAGCG	GCAGCGACAGCG	8: 84,956,172	probably benign	Het
Setd1a	GTGGTGGT	GTGGTGGTATTGGTGGT	7: 127,785,318	probably benign	Het
Treml1	ACCT	A	17: 48,359,947	probably null	Het
Triobp	CTCCCTGTGCCCAAC	CTCCCTGTGCCCAACTGAACAACCCCAGGATTCCCTGTGCCCAAC	15: 78,967,044	probably benign	Het
Zfp598	CCCACCACCACAACCACCACCACCACCACCAC	CCCACCACCACCACCACAACCACCACCACCACCACCAC	17: 24,680,761	probably benign	Het

Other mutations in Krtap28-10

Allele	Source	Chr	Coord	Type	Predicted Effect
FR4737:Krtap28-10	UTSW	1	83042123	unclassified	probably benign
R8865:Krtap28-10	UTSW	1	83042087	missense	unknown
R8984:Krtap28-10	UTSW	1	83042173	missense	unknown
RF001:Krtap28-10	UTSW	1	83042255	unclassified	probably benign
RF001:Krtap28-10	UTSW	1	83042280	unclassified	probably benign
RF001:Krtap28-10	UTSW	1	83042282	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83042128	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83042135	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83042253	unclassified	probably benign
RF008:Krtap28-10	UTSW	1	83042279	unclassified	probably benign
RF012:Krtap28-10	UTSW	1	83042136	unclassified	probably benign
RF013:Krtap28-10	UTSW	1	83042135	unclassified	probably benign
RF013:Krtap28-10	UTSW	1	83042274	unclassified	probably benign
RF014:Krtap28-10	UTSW	1	83042251	unclassified	probably benign
RF016:Krtap28-10	UTSW	1	83042123	unclassified	probably benign
RF017:Krtap28-10	UTSW	1	83042138	unclassified	probably benign
RF017:Krtap28-10	UTSW	1	83042266	unclassified	probably benign
RF018:Krtap28-10	UTSW	1	83042253	unclassified	probably benign
RF019:Krtap28-10	UTSW	1	83042269	unclassified	probably benign
RF023:Krtap28-10	UTSW	1	83042146	nonsense	probably null
RF023:Krtap28-10	UTSW	1	83042286	unclassified	probably benign
RF024:Krtap28-10	UTSW	1	83042123	unclassified	probably benign
RF024:Krtap28-10	UTSW	1	83042252	unclassified	probably benign
RF025:Krtap28-10	UTSW	1	83042258	unclassified	probably benign
RF026:Krtap28-10	UTSW	1	83042126	unclassified	probably benign
RF027:Krtap28-10	UTSW	1	83042285	unclassified	probably benign
RF028:Krtap28-10	UTSW	1	83042258	unclassified	probably benign
RF029:Krtap28-10	UTSW	1	83042270	unclassified	probably benign
RF032:Krtap28-10	UTSW	1	83042258	unclassified	probably benign
RF034:Krtap28-10	UTSW	1	83042282	unclassified	probably benign
RF035:Krtap28-10	UTSW	1	83042146	unclassified	probably benign
RF035:Krtap28-10	UTSW	1	83042281	unclassified	probably benign
RF037:Krtap28-10	UTSW	1	83042145	unclassified	probably benign
RF037:Krtap28-10	UTSW	1	83042286	unclassified	probably benign
RF038:Krtap28-10	UTSW	1	83042128	unclassified	probably benign
RF038:Krtap28-10	UTSW	1	83042257	unclassified	probably benign
RF042:Krtap28-10	UTSW	1	83042125	unclassified	probably benign
RF044:Krtap28-10	UTSW	1	83042131	unclassified	probably benign
RF045:Krtap28-10	UTSW	1	83042143	unclassified	probably benign
RF045:Krtap28-10	UTSW	1	83042261	unclassified	probably benign
RF049:Krtap28-10	UTSW	1	83042138	unclassified	probably benign
RF049:Krtap28-10	UTSW	1	83042285	unclassified	probably benign
RF053:Krtap28-10	UTSW	1	83042278	unclassified	probably benign
RF055:Krtap28-10	UTSW	1	83042130	unclassified	probably benign
RF055:Krtap28-10	UTSW	1	83042262	unclassified	probably benign
RF055:Krtap28-10	UTSW	1	83042270	unclassified	probably benign
RF059:Krtap28-10	UTSW	1	83042275	unclassified	probably benign
RF059:Krtap28-10	UTSW	1	83042290	unclassified	probably benign
RF061:Krtap28-10	UTSW	1	83042281	unclassified	probably benign
RF064:Krtap28-10	UTSW	1	83042131	unclassified	probably benign
Z1177:Krtap28-10	UTSW	1	83042159	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCGTAGCTTAGAACCAGAGCC -3'
(R):5'- TCCTCTCTACTGACAACATGGG -3'

Sequencing Primer
(F):5'- GCTAGCAGCAGCACTTCTTG -3'
(R):5'- AACATGGGTTGTTGTGGCTG -3'

Posted On

2019-12-04