Mutagenetix > Incidental Mutation

Incidental Mutation 'R7835:Rgs22'

605940

Institutional Source

Beutler Lab

Gene Symbol

Rgs22

Ensembl Gene

ENSMUSG00000037627

Gene Name

regulator of G-protein signalling 22

Synonyms

MMRRC Submission

045889-MU

Accession Numbers

Genbank: NM_001195748; MGI: 3613651

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7835 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

36009479-36140400 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 36081911 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134259 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172831]

AlphaFold

G3UYX5

Predicted Effect

probably null
Transcript: ENSMUST00000172737

SMART Domains

Protein: ENSMUSP00000133508
Gene: ENSMUSG00000037627

Domain	Start	End	E-Value	Type
Blast:RGS	231	262	1e-13	BLAST

Predicted Effect

probably null
Transcript: ENSMUST00000172831

SMART Domains

Protein: ENSMUSP00000134259
Gene: ENSMUSG00000037627

Domain	Start	End	E-Value	Type
low complexity region	9	19	N/A	INTRINSIC
low complexity region	62	76	N/A	INTRINSIC
low complexity region	173	179	N/A	INTRINSIC
low complexity region	376	391	N/A	INTRINSIC
RGS	845	973	3.15e-2	SMART
RGS	1014	1134	1.56e-15	SMART

Meta Mutation Damage Score

0.9491

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

98% (48/49)

Allele List at MGI

All alleles(2) : Targeted, other(2)

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	T	A	7: 79,099,875	S1465T	probably benign	Het
Accsl	T	A	2: 93,865,984	K90*	probably null	Het
Adamts3	A	T	5: 89,700,440	D674E	possibly damaging	Het
Adap2	T	A	11: 80,160,231	V129D	probably benign	Het
Ank3	A	G	10: 69,987,727	D742G		Het
C1ra	G	A	6: 124,517,725	E316K	probably benign	Het
C330021F23Rik	A	G	8: 3,580,452		probably benign	Het
Cachd1	G	T	4: 100,974,153		probably null	Het
Ccne1	G	T	7: 38,102,845	Q133K	probably benign	Het
Cers3	C	T	7: 66,773,639	H111Y	possibly damaging	Het
Chst5	A	T	8: 111,890,602	L129M	probably damaging	Het
Depdc7	A	G	2: 104,728,185	S164P	probably benign	Het
Dnah10	G	A	5: 124,777,234	A1966T	probably damaging	Het
Fam208a	G	T	14: 27,476,643	G1311C	probably damaging	Het
Fcgbp	T	G	7: 28,117,207	S2365A	possibly damaging	Het
Ihh	A	G	1: 74,946,366	V320A	probably damaging	Het
Kdm5d	T	C	Y: 900,558	V201A	possibly damaging	Het
Kif13b	T	A	14: 64,767,452	H1117Q	probably benign	Het
Lcn8	C	A	2: 25,655,296		probably null	Het
Lrp4	T	C	2: 91,495,042	V1404A	possibly damaging	Het
Lrrc61	A	T	6: 48,568,572	T110S	probably benign	Het
Mrpl19	G	A	6: 81,962,126	R232C	probably damaging	Het
Muc5ac	G	C	7: 141,809,303	G2117A	unknown	Het
Mzt1	T	C	14: 99,046,003	T21A	probably benign	Het
Naip6	G	T	13: 100,316,004	A183E	probably benign	Het
Neb	T	C	2: 52,150,577	D6624G	probably benign	Het
Nup85	A	G	11: 115,570,071	D183G	probably benign	Het
Olfm5	A	T	7: 104,154,445	Y195*	probably null	Het
Olfr828	T	A	9: 18,815,809	M162L	probably benign	Het
Piezo2	A	T	18: 63,082,945	F1216I	probably benign	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195		probably benign	Het
Polr1a	T	C	6: 71,915,142	V135A	probably benign	Het
Ppcdc	A	G	9: 57,420,276	S83P	probably benign	Het
Prss21	A	T	17: 23,869,451	Q130L	possibly damaging	Het
Rdx	T	A	9: 52,065,788	N112K	probably damaging	Het
Rps26	C	T	10: 128,626,126	V40I	probably benign	Het
Runx2	A	T	17: 44,608,236	M405K	probably damaging	Het
Serinc2	A	C	4: 130,275,487	C4G	unknown	Het
Sh3rf2	C	A	18: 42,111,170	R266S	probably benign	Het
Slc38a10	A	G	11: 120,116,996	I386T	possibly damaging	Het
Stab2	G	A	10: 86,872,619	P1694L	probably benign	Het
Taf4	G	A	2: 179,932,029	T682M	probably damaging	Het
Tmem102	A	G	11: 69,804,345	V267A	probably damaging	Het
Trim65	A	G	11: 116,130,929	L26P	probably damaging	Het
Vmn1r160	T	A	7: 22,871,954	M244K	possibly damaging	Het
Vmn1r57	A	T	7: 5,221,139	H221L	probably benign	Het
Wdr89	C	A	12: 75,632,899	V194F	probably damaging	Het
Wee1	C	A	7: 110,130,878	Y396*	probably null	Het
Zfp451	A	T	1: 33,772,979	V885D	probably damaging	Het
Zfp981	A	T	4: 146,537,876	Q419H	probably benign	Het
Znfx1	A	G	2: 167,039,827	Y1081H	probably damaging	Het

Other mutations in Rgs22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00164:Rgs22	APN	15	36,099,931 (GRCm38)	missense	possibly damaging	0.93
IGL00594:Rgs22	APN	15	36,083,631 (GRCm38)	missense	probably benign	0.00
IGL01464:Rgs22	APN	15	36,083,641 (GRCm38)	missense	possibly damaging	0.90
IGL01686:Rgs22	APN	15	36,103,835 (GRCm38)	missense	probably benign	0.00
IGL01761:Rgs22	APN	15	36,103,751 (GRCm38)	missense	probably damaging	0.99
IGL02045:Rgs22	APN	15	36,013,154 (GRCm38)	missense	probably benign	0.33
IGL02378:Rgs22	APN	15	36,103,805 (GRCm38)	missense	probably benign	0.00
IGL02490:Rgs22	APN	15	36,054,847 (GRCm38)	missense	probably damaging	1.00
IGL03219:Rgs22	APN	15	36,107,048 (GRCm38)	missense	probably damaging	1.00
IGL03229:Rgs22	APN	15	36,015,779 (GRCm38)	splice site	probably benign
IGL03328:Rgs22	APN	15	36,043,204 (GRCm38)	critical splice donor site	probably null
3-1:Rgs22	UTSW	15	36,100,036 (GRCm38)	missense	possibly damaging	0.48
R0254:Rgs22	UTSW	15	36,104,552 (GRCm38)	missense	probably damaging	0.99
R0463:Rgs22	UTSW	15	36,092,938 (GRCm38)	missense	probably damaging	1.00
R0467:Rgs22	UTSW	15	36,099,795 (GRCm38)	nonsense	probably null
R0486:Rgs22	UTSW	15	36,092,882 (GRCm38)	missense	probably damaging	0.98
R0554:Rgs22	UTSW	15	36,054,709 (GRCm38)	missense	probably benign	0.10
R0602:Rgs22	UTSW	15	36,139,872 (GRCm38)	splice site	probably benign
R0906:Rgs22	UTSW	15	36,103,902 (GRCm38)	intron	probably benign
R1159:Rgs22	UTSW	15	36,040,693 (GRCm38)	missense	probably damaging	1.00
R1300:Rgs22	UTSW	15	36,101,762 (GRCm38)	missense	probably benign	0.43
R1439:Rgs22	UTSW	15	36,025,793 (GRCm38)	splice site	probably benign
R1491:Rgs22	UTSW	15	36,092,901 (GRCm38)	missense	probably damaging	0.98
R1502:Rgs22	UTSW	15	36,080,851 (GRCm38)	missense	probably damaging	1.00
R1514:Rgs22	UTSW	15	36,013,100 (GRCm38)	missense	probably benign	0.00
R1538:Rgs22	UTSW	15	36,048,776 (GRCm38)	missense	probably damaging	1.00
R1784:Rgs22	UTSW	15	36,087,436 (GRCm38)	missense	probably damaging	1.00
R1938:Rgs22	UTSW	15	36,101,804 (GRCm38)	missense	probably benign	0.00
R1972:Rgs22	UTSW	15	36,103,836 (GRCm38)	missense	probably benign	0.01
R2109:Rgs22	UTSW	15	36,099,734 (GRCm38)	nonsense	probably null
R2208:Rgs22	UTSW	15	36,050,232 (GRCm38)	missense	probably benign	0.01
R3696:Rgs22	UTSW	15	36,099,892 (GRCm38)	missense	probably benign	0.00
R3697:Rgs22	UTSW	15	36,099,892 (GRCm38)	missense	probably benign	0.00
R3698:Rgs22	UTSW	15	36,099,892 (GRCm38)	missense	probably benign	0.00
R3879:Rgs22	UTSW	15	36,106,905 (GRCm38)	missense	possibly damaging	0.52
R4080:Rgs22	UTSW	15	36,107,076 (GRCm38)	missense	probably damaging	1.00
R4363:Rgs22	UTSW	15	36,103,874 (GRCm38)	missense	probably damaging	0.99
R4591:Rgs22	UTSW	15	36,100,136 (GRCm38)	missense	probably benign	0.01
R4673:Rgs22	UTSW	15	36,099,933 (GRCm38)	missense	probably benign	0.04
R4829:Rgs22	UTSW	15	36,103,888 (GRCm38)	missense	probably damaging	1.00
R4831:Rgs22	UTSW	15	36,050,148 (GRCm38)	missense	probably benign	0.00
R4865:Rgs22	UTSW	15	36,100,212 (GRCm38)	missense	probably damaging	1.00
R4907:Rgs22	UTSW	15	36,087,424 (GRCm38)	missense	possibly damaging	0.61
R4944:Rgs22	UTSW	15	36,025,942 (GRCm38)	missense	possibly damaging	0.83
R4975:Rgs22	UTSW	15	36,054,876 (GRCm38)	nonsense	probably null
R5056:Rgs22	UTSW	15	36,050,245 (GRCm38)	splice site	probably null
R5126:Rgs22	UTSW	15	36,040,644 (GRCm38)	missense	probably damaging	0.96
R5138:Rgs22	UTSW	15	36,099,788 (GRCm38)	missense	probably benign	0.04
R5444:Rgs22	UTSW	15	36,015,627 (GRCm38)	missense	possibly damaging	0.83
R5507:Rgs22	UTSW	15	36,099,652 (GRCm38)	missense	probably damaging	0.99
R5640:Rgs22	UTSW	15	36,106,955 (GRCm38)	missense	probably benign	0.00
R5969:Rgs22	UTSW	15	36,015,636 (GRCm38)	missense	probably benign	0.00
R6005:Rgs22	UTSW	15	36,010,567 (GRCm38)	missense	probably benign	0.39
R6053:Rgs22	UTSW	15	36,100,007 (GRCm38)	missense	probably benign	0.04
R6134:Rgs22	UTSW	15	36,107,048 (GRCm38)	missense	probably damaging	1.00
R6230:Rgs22	UTSW	15	36,100,030 (GRCm38)	missense	probably benign	0.02
R6295:Rgs22	UTSW	15	36,087,374 (GRCm38)	missense	probably benign	0.00
R6352:Rgs22	UTSW	15	36,092,921 (GRCm38)	missense	probably damaging	1.00
R6809:Rgs22	UTSW	15	36,048,764 (GRCm38)	missense	probably damaging	1.00
R6900:Rgs22	UTSW	15	36,010,747 (GRCm38)	missense	possibly damaging	0.61
R6947:Rgs22	UTSW	15	36,103,890 (GRCm38)	critical splice acceptor site	probably null
R7102:Rgs22	UTSW	15	36,122,313 (GRCm38)	missense	probably damaging	1.00
R7126:Rgs22	UTSW	15	36,103,808 (GRCm38)	missense	probably damaging	0.97
R7263:Rgs22	UTSW	15	36,015,643 (GRCm38)	missense	possibly damaging	0.86
R7623:Rgs22	UTSW	15	36,040,710 (GRCm38)	missense	probably benign	0.08
R7732:Rgs22	UTSW	15	36,025,981 (GRCm38)	missense	probably damaging	1.00
R7748:Rgs22	UTSW	15	36,122,269 (GRCm38)	critical splice donor site	probably null
R7771:Rgs22	UTSW	15	36,050,078 (GRCm38)	missense	possibly damaging	0.94
R7849:Rgs22	UTSW	15	36,099,712 (GRCm38)	missense	probably damaging	1.00
R7954:Rgs22	UTSW	15	36,082,002 (GRCm38)	missense	possibly damaging	0.75
R8384:Rgs22	UTSW	15	36,046,012 (GRCm38)	critical splice donor site	probably null
R8516:Rgs22	UTSW	15	36,010,335 (GRCm38)	makesense	probably null
R8904:Rgs22	UTSW	15	36,025,981 (GRCm38)	missense	probably damaging	1.00
R8923:Rgs22	UTSW	15	36,092,960 (GRCm38)	missense	probably damaging	1.00
R9287:Rgs22	UTSW	15	36,098,263 (GRCm38)	missense	probably damaging	1.00
R9324:Rgs22	UTSW	15	36,087,398 (GRCm38)	missense	probably benign
R9660:Rgs22	UTSW	15	36,040,710 (GRCm38)	missense	probably benign	0.08
R9679:Rgs22	UTSW	15	36,087,441 (GRCm38)	missense	probably benign	0.00
R9728:Rgs22	UTSW	15	36,040,710 (GRCm38)	missense	probably benign	0.08
RF035:Rgs22	UTSW	15	36,010,835 (GRCm38)	critical splice acceptor site	probably benign
RF043:Rgs22	UTSW	15	36,010,836 (GRCm38)	critical splice acceptor site	probably benign

Predicted Primers

PCR Primer
(F):5'- GACCAAAGAATTACAATTTGGGCAG -3'
(R):5'- ACAGTCAGATGCAAACACATTG -3'

Sequencing Primer
(F):5'- GGGCAGATGGTTAAAATTTTTCTAAG -3'
(R):5'- AGCCTTGGGAGGATTTGA -3'

Posted On

2019-12-20