Mutagenetix > Incidental Mutation

Incidental Mutation 'R7838:Or2y3'

606132

Institutional Source

Beutler Lab

Gene Symbol

Or2y3

Ensembl Gene

ENSMUSG00000043312

Gene Name

olfactory receptor family 2 subfamily Y member 3

Synonyms

GA_x6K02T2PSCP-2531299-2530355, MOR256-4, Olfr131

MMRRC Submission

045892-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7838 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38392923-38393867 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 38393293 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 192 (V192A)

Ref Sequence

ENSEMBL: ENSMUSP00000134532 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059560] [ENSMUST00000172933] [ENSMUST00000215940] [ENSMUST00000216523]

AlphaFold

Q8VGC8

Predicted Effect

probably benign
Transcript: ENSMUST00000059560
AA Change: V192A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000061861
Gene: ENSMUSG00000043312
AA Change: V192A

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	294	4e-35	PFAM
Pfam:7tm_4	141	287	2.7e-40	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172933
AA Change: V192A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000134532
Gene: ENSMUSG00000043312
AA Change: V192A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	312	9.5e-57	PFAM
Pfam:7tm_1	43	294	1.2e-29	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215940
AA Change: V192A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000216523
AA Change: V192A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

98% (64/65)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	G	A	10: 87,042,061 (GRCm39)		probably null	Het
4930522H14Rik	G	A	4: 109,362,776 (GRCm39)	A181V	probably damaging	Het
Alk	A	T	17: 72,274,549 (GRCm39)	H587Q	possibly damaging	Het
Ascc3	A	G	10: 50,604,393 (GRCm39)	Y1371C	probably benign	Het
Asxl1	T	C	2: 153,238,733 (GRCm39)	F354S	probably damaging	Het
Cachd1	A	T	4: 100,824,211 (GRCm39)	I551F	possibly damaging	Het
Cacna1e	T	C	1: 154,347,149 (GRCm39)	E1068G	probably benign	Het
Cdc27	A	T	11: 104,403,830 (GRCm39)	M644K	probably damaging	Het
Chl1	T	C	6: 103,668,635 (GRCm39)	V456A	probably benign	Het
Ctxn1	A	G	8: 4,308,461 (GRCm39)	Y57H	probably damaging	Het
Cyp2c54	A	G	19: 40,058,688 (GRCm39)	I248T	probably benign	Het
Dchs2	A	G	3: 83,211,834 (GRCm39)	T1878A	probably benign	Het
Ddi2	A	G	4: 141,412,561 (GRCm39)	V117A	probably benign	Het
Dennd5a	G	T	7: 109,533,196 (GRCm39)	F191L	probably benign	Het
Dnm3	C	T	1: 161,819,619 (GRCm39)	R851H	possibly damaging	Het
Eif3l	G	A	15: 78,973,799 (GRCm39)	D405N	possibly damaging	Het
Espn	T	A	4: 152,215,738 (GRCm39)	D507V	possibly damaging	Het
Fam83f	A	T	15: 80,576,704 (GRCm39)	S452C	possibly damaging	Het
Fbxo22	A	C	9: 55,125,651 (GRCm39)	E171D	probably damaging	Het
Fgl2	T	C	5: 21,577,752 (GRCm39)	V13A	probably benign	Het
Fsip2	A	G	2: 82,807,044 (GRCm39)	H1121R	probably benign	Het
G0s2	A	T	1: 192,955,081 (GRCm39)	M1K	probably null	Het
Gnptab	G	A	10: 88,276,254 (GRCm39)		probably null	Het
Grm1	G	A	10: 10,956,096 (GRCm39)	P63S	probably benign	Het
Grsf1	G	A	5: 88,823,523 (GRCm39)		probably benign	Het
Hcfc1r1	G	A	17: 23,892,985 (GRCm39)	G10D	probably damaging	Het
Hdac1-ps	A	C	17: 78,799,447 (GRCm39)	E146A	probably damaging	Het
Igsf11	T	C	16: 38,827,565 (GRCm39)	V41A	possibly damaging	Het
Il17a	C	A	1: 20,802,351 (GRCm39)	A20E	probably benign	Het
Kcnv2	A	G	19: 27,300,332 (GRCm39)	Y61C	probably damaging	Het
Kmt2c	A	G	5: 25,499,697 (GRCm39)	M481T	possibly damaging	Het
Krt40	A	T	11: 99,430,961 (GRCm39)	C263S	possibly damaging	Het
Larp1	T	C	11: 57,938,540 (GRCm39)	V441A	possibly damaging	Het
Lrwd1	T	C	5: 136,160,983 (GRCm39)	E300G	probably damaging	Het
Mcoln3	G	T	3: 145,845,230 (GRCm39)	W475C	probably damaging	Het
Mdga1	A	G	17: 30,058,796 (GRCm39)	I30T	probably benign	Het
Mmd	T	A	11: 90,158,433 (GRCm39)	V181D	probably benign	Het
Muc4	T	A	16: 32,752,558 (GRCm38)	L812*	probably null	Het
Mycbp2	T	C	14: 103,414,729 (GRCm39)	D2561G	probably benign	Het
Myh11	T	A	16: 14,027,481 (GRCm39)	E1251V		Het
Myl12a	G	T	17: 71,303,166 (GRCm39)	N95K	probably benign	Het
Myo1h	A	G	5: 114,466,872 (GRCm39)		probably null	Het
Myrf	G	A	19: 10,196,983 (GRCm39)	P266S	possibly damaging	Het
Ncf1	A	G	5: 134,250,949 (GRCm39)	V330A	possibly damaging	Het
Nfasc	T	C	1: 132,533,287 (GRCm39)	D638G	probably damaging	Het
Nkapl	T	C	13: 21,651,437 (GRCm39)	K392R	possibly damaging	Het
Or6a2	A	T	7: 106,600,514 (GRCm39)	C184*	probably null	Het
Or8b50	A	G	9: 38,517,708 (GRCm39)		probably benign	Het
Pelo	T	A	13: 115,226,184 (GRCm39)	N91I	probably damaging	Het
Ptk2b	T	C	14: 66,395,850 (GRCm39)	N836D	probably benign	Het
Pwp2	A	T	10: 78,018,778 (GRCm39)		probably null	Het
Slc44a1	G	A	4: 53,517,657 (GRCm39)	V127I	probably benign	Het
Snx13	A	G	12: 35,155,174 (GRCm39)	T413A	probably benign	Het
Spaca7	C	T	8: 12,635,696 (GRCm39)	P71S	probably damaging	Het
Spef2	A	T	15: 9,609,637 (GRCm39)	M1296K	possibly damaging	Het
Suco	G	A	1: 161,656,890 (GRCm39)	A978V	probably benign	Het
Tcerg1	A	G	18: 42,670,002 (GRCm39)	E494G	probably benign	Het
Tenm2	A	G	11: 35,997,626 (GRCm39)	V821A	probably benign	Het
Tg	T	A	15: 66,565,112 (GRCm39)	D1151E	probably benign	Het
Trp53bp2	A	G	1: 182,283,384 (GRCm39)	E1040G	probably damaging	Het
Ubap2	A	T	4: 41,233,655 (GRCm39)	N79K	probably damaging	Het
Vmn2r111	T	C	17: 22,778,032 (GRCm39)	N549S	possibly damaging	Het
Yars2	T	G	16: 16,122,385 (GRCm39)		probably null	Het
Ylpm1	A	G	12: 85,095,640 (GRCm39)	I1847V	possibly damaging	Het

Other mutations in Or2y3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01063:Or2y3	APN	17	38,393,544 (GRCm39)	missense	possibly damaging	0.48
IGL01287:Or2y3	APN	17	38,392,998 (GRCm39)	missense	probably damaging	1.00
IGL01637:Or2y3	APN	17	38,392,994 (GRCm39)	missense	possibly damaging	0.90
IGL02833:Or2y3	APN	17	38,393,243 (GRCm39)	missense	possibly damaging	0.84
IGL02927:Or2y3	APN	17	38,393,114 (GRCm39)	missense	probably benign	0.00
R0662:Or2y3	UTSW	17	38,393,824 (GRCm39)	missense	probably benign	0.09
R0755:Or2y3	UTSW	17	38,393,085 (GRCm39)	nonsense	probably null
R1526:Or2y3	UTSW	17	38,393,486 (GRCm39)	missense	probably damaging	0.99
R2155:Or2y3	UTSW	17	38,393,071 (GRCm39)	missense	probably damaging	1.00
R3123:Or2y3	UTSW	17	38,392,903 (GRCm39)	splice site	probably null
R3125:Or2y3	UTSW	17	38,392,903 (GRCm39)	splice site	probably null
R4135:Or2y3	UTSW	17	38,393,248 (GRCm39)	missense	possibly damaging	0.52
R4244:Or2y3	UTSW	17	38,393,321 (GRCm39)	missense	probably benign	0.12
R5104:Or2y3	UTSW	17	38,393,174 (GRCm39)	missense	possibly damaging	0.47
R5182:Or2y3	UTSW	17	38,393,005 (GRCm39)	missense	probably benign	0.03
R5293:Or2y3	UTSW	17	38,393,131 (GRCm39)	missense	probably damaging	1.00
R5738:Or2y3	UTSW	17	38,393,347 (GRCm39)	missense	probably damaging	1.00
R5924:Or2y3	UTSW	17	38,393,254 (GRCm39)	missense	probably benign	0.01
R6218:Or2y3	UTSW	17	38,393,620 (GRCm39)	missense	probably damaging	0.98
R6362:Or2y3	UTSW	17	38,393,620 (GRCm39)	missense	probably damaging	0.99
R6961:Or2y3	UTSW	17	38,393,096 (GRCm39)	missense	probably damaging	0.99
R8088:Or2y3	UTSW	17	38,393,452 (GRCm39)	missense	possibly damaging	0.78
R8730:Or2y3	UTSW	17	38,392,925 (GRCm39)	makesense	probably null
R9303:Or2y3	UTSW	17	38,393,629 (GRCm39)	missense	probably damaging	1.00
R9305:Or2y3	UTSW	17	38,393,629 (GRCm39)	missense	probably damaging	1.00
R9378:Or2y3	UTSW	17	38,393,056 (GRCm39)	missense	possibly damaging	0.92
R9776:Or2y3	UTSW	17	38,393,470 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GCTCTTCGGTGCCATGTATG -3'
(R):5'- ACAGTTATCATGCATCCCCAGC -3'

Sequencing Primer
(F):5'- TGCATATCCACTGATGGCAG -3'
(R):5'- ATGCATCCCCAGCTGTGC -3'

Posted On

2019-12-20