Mutagenetix > Incidental Mutation

Incidental Mutation 'R7842:Myo15b'

606392

Institutional Source

Beutler Lab

Gene Symbol

Myo15b

Ensembl Gene

ENSMUSG00000034427

Gene Name

myosin XVB

Synonyms

LOC217328, E330039G21Rik, LOC380737

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R7842 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

115858406-115892603 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 115871495 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1214 (T1214A)

Ref Sequence

ENSEMBL: ENSMUSP00000091439 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093911]

AlphaFold

no structure available at present

Predicted Effect

SMART Domains

Protein: ENSMUSP00000091439
Gene: ENSMUSG00000034427
AA Change: T1214A

Domain	Start	End	E-Value	Type
MYSc	1	640	2.4e-134	SMART
IQ	660	682	1.03e1	SMART
Pfam:MyTH4	837	945	2.1e-23	PFAM
low complexity region	1050	1068	N/A	INTRINSIC
low complexity region	1136	1170	N/A	INTRINSIC
low complexity region	1207	1246	N/A	INTRINSIC
low complexity region	1302	1327	N/A	INTRINSIC
low complexity region	1454	1468	N/A	INTRINSIC
low complexity region	1489	1509	N/A	INTRINSIC
SH3	1735	1792	1.15e-7	SMART
Pfam:MyTH4	1928	2029	8.3e-25	PFAM
B41	2032	2235	6.99e-4	SMART
low complexity region	2243	2253	N/A	INTRINSIC

Meta Mutation Damage Score

0.0852

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (77/78)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034J05Rik	T	C	6: 146,953,536	T3A	unknown	Het
4933412E24Rik	T	C	15: 60,016,573	E6G	probably damaging	Het
Abca6	A	G	11: 110,196,697	L1135P	possibly damaging	Het
Agps	A	G	2: 75,851,532	D113G	probably damaging	Het
Ankhd1	G	T	18: 36,647,828	V1978L	probably benign	Het
Ankrd31	T	A	13: 96,821,458		probably null	Het
Arhgap5	T	A	12: 52,518,697	M817K	possibly damaging	Het
Bdp1	C	A	13: 100,099,129	V105F	probably damaging	Het
Brdt	A	G	5: 107,348,588	N189D	possibly damaging	Het
Ccdc180	A	T	4: 45,909,428	N532I	probably benign	Het
Ccnc	A	C	4: 21,730,480	K39T	probably damaging	Het
Cfap57	C	A	4: 118,554,755	G1231*	probably null	Het
Chd7	A	T	4: 8,854,115	T1896S	probably benign	Het
Clk4	A	T	11: 51,281,129	H412L	probably benign	Het
Creld1	T	C	6: 113,488,139	L109P	probably damaging	Het
Cxxc4	A	T	3: 134,240,332	I225L	possibly damaging	Het
Cyp4f39	A	T	17: 32,483,317	R263W	probably benign	Het
Dip2c	T	C	13: 9,606,533		probably null	Het
Dnah14	A	G	1: 181,627,898	T863A	probably damaging	Het
Dnah17	C	A	11: 118,079,682		probably null	Het
Dnajc10	A	G	2: 80,345,065	K599E	probably benign	Het
Dnajc7	C	A	11: 100,598,718	R101L	probably benign	Het
Dnttip2	G	A	3: 122,276,341	E402K	probably benign	Het
Dusp22	T	A	13: 30,668,791		probably null	Het
Egflam	C	A	15: 7,251,194	R450M	probably null	Het
Eml5	C	T	12: 98,794,135	R1785Q	probably damaging	Het
Fam205c	TCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGTCATTCAACACTTTGG	TCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGTCATTCAACACTTTGG	4: 42,871,823		probably benign	Het
Fam45a	G	A	19: 60,830,879	A263T	not run	Het
Fam78b	G	T	1: 167,001,609	R15L	probably damaging	Het
Fbxl5	A	T	5: 43,758,603	I489N	probably damaging	Het
Flt3	G	A	5: 147,334,453	P893S	probably damaging	Het
Gm17019	T	C	5: 15,031,035	M131V	possibly damaging	Het
Gm21560	T	A	14: 6,216,262	I194F	probably benign	Het
Gm28729	T	C	9: 96,517,652	D158G	probably damaging	Het
Gucy2c	C	T	6: 136,769,816		probably null	Het
Hectd1	C	T	12: 51,772,560	G1247S	probably damaging	Het
Ireb2	T	A	9: 54,909,686	I946K	probably benign	Het
Itgb4	A	T	11: 115,982,705	D249V	probably benign	Het
Klhdc7a	A	G	4: 139,967,238	S133P	probably damaging	Het
Lsr	A	T	7: 30,966,012	D172E	possibly damaging	Het
Macf1	T	C	4: 123,526,909	E184G	probably benign	Het
Map2	T	A	1: 66,416,411	D1421E	probably benign	Het
Map3k4	A	T	17: 12,271,143	L467H	possibly damaging	Het
Mogat1	T	C	1: 78,522,865		probably null	Het
Mxra8	A	G	4: 155,842,910	D384G	probably damaging	Het
Naip1	T	C	13: 100,426,998	Y553C	probably damaging	Het
Nln	A	T	13: 104,052,629	I278K	probably benign	Het
Nsmaf	T	A	4: 6,435,109		probably null	Het
Nynrin	A	G	14: 55,865,096	R741G	probably damaging	Het
Olfr1045	G	T	2: 86,198,172	Y193*	probably null	Het
Olfr1166	C	T	2: 88,124,986		probably benign	Het
Olfr1208	A	G	2: 88,896,961	V212A	possibly damaging	Het
Olfr603	A	G	7: 103,383,576	V142A	probably benign	Het
Orc1	A	G	4: 108,605,547	H607R	probably benign	Het
Pate2	A	T	9: 35,670,533	Y41F	probably damaging	Het
Pcdhb20	A	T	18: 37,505,059	T213S	possibly damaging	Het
Pcm1	G	A	8: 41,327,584	E1858K	possibly damaging	Het
Pla2g2d	A	G	4: 138,778,778	T59A	probably damaging	Het
Ppl	C	T	16: 5,088,861	R1190H	probably damaging	Het
Prl2c2	A	G	13: 13,005,322	L2P	probably benign	Het
Proz	G	A	8: 13,063,406	V76I	probably benign	Het
Prr36	A	G	8: 4,210,953	S955P	probably benign	Het
Pter	T	C	2: 12,978,541	V119A	probably damaging	Het
Qrich1	T	A	9: 108,556,368		probably null	Het
Rbm15b	T	C	9: 106,885,889	E360G	probably damaging	Het
Skint11	G	A	4: 114,244,771	C327Y	possibly damaging	Het
Spag17	T	A	3: 100,053,858	S987T	probably benign	Het
Tbx1	T	C	16: 18,586,615	S100G	unknown	Het
Tiam2	C	T	17: 3,518,124	S1515L	possibly damaging	Het
Togaram1	C	A	12: 64,966,459	D161E	probably damaging	Het
Trhde	A	T	10: 114,696,098	S366T	possibly damaging	Het
Ttc12	A	G	9: 49,438,424	I691T	possibly damaging	Het
Ube4a	A	G	9: 44,949,727		probably null	Het
Uggt2	A	T	14: 118,998,104	I1453N	probably damaging	Het
Unc79	T	C	12: 103,092,054	S972P	probably damaging	Het
Wdr20	G	A	12: 110,738,215	D63N	probably benign	Het
Wdr66	T	C	5: 123,254,424	V207A	unknown	Het
Wls	T	A	3: 159,873,179	I126N	probably benign	Het
Xirp2	A	G	2: 67,524,945	D3350G	probably benign	Het

Other mutations in Myo15b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00556:Myo15b	APN	11	115891916	missense	possibly damaging	0.69
IGL01409:Myo15b	APN	11	115869504	nonsense	probably null
IGL01539:Myo15b	APN	11	115863473	missense	probably benign	0.43
IGL01895:Myo15b	APN	11	115883498	missense	possibly damaging	0.77
IGL02254:Myo15b	APN	11	115886283	missense	probably damaging	1.00
IGL02343:Myo15b	APN	11	115873400	unclassified	probably benign
IGL02349:Myo15b	APN	11	115863105	splice site	probably benign
IGL02368:Myo15b	APN	11	115877002	missense	probably benign	0.13
IGL02576:Myo15b	APN	11	115890053	missense	probably null	0.97
IGL02650:Myo15b	APN	11	115886511	critical splice donor site	probably null
IGL02661:Myo15b	APN	11	115884069	missense	probably benign	0.01
IGL02716:Myo15b	APN	11	115883709	missense	probably benign	0.06
IGL02733:Myo15b	APN	11	115884250	missense	probably benign	0.00
IGL02951:Myo15b	APN	11	115881301	missense	probably damaging	1.00
IGL03017:Myo15b	APN	11	115887917	missense	possibly damaging	0.91
IGL03029:Myo15b	APN	11	115871643	missense	probably benign	0.08
ANU74:Myo15b	UTSW	11	115878413	missense	probably damaging	1.00
R0092:Myo15b	UTSW	11	115862986	missense	possibly damaging	0.90
R0255:Myo15b	UTSW	11	115886283	missense	probably damaging	1.00
R0325:Myo15b	UTSW	11	115884265	missense	probably damaging	1.00
R0614:Myo15b	UTSW	11	115882913	missense	probably damaging	1.00
R0652:Myo15b	UTSW	11	115864642	missense	probably benign	0.07
R0711:Myo15b	UTSW	11	115883838	missense	probably damaging	1.00
R0815:Myo15b	UTSW	11	115866336	splice site	probably benign
R0961:Myo15b	UTSW	11	115882454	missense	probably benign	0.15
R1066:Myo15b	UTSW	11	115879751	missense	probably benign	0.03
R1221:Myo15b	UTSW	11	115886720	missense	possibly damaging	0.75
R1240:Myo15b	UTSW	11	115880501	missense	possibly damaging	0.70
R1275:Myo15b	UTSW	11	115883492	small deletion	probably benign
R1313:Myo15b	UTSW	11	115885129	missense	probably damaging	1.00
R1313:Myo15b	UTSW	11	115885129	missense	probably damaging	1.00
R1317:Myo15b	UTSW	11	115883634	missense	probably null	0.14
R1491:Myo15b	UTSW	11	115886857	splice site	probably null
R1552:Myo15b	UTSW	11	115866635	missense	probably benign	0.08
R1731:Myo15b	UTSW	11	115891560	missense	possibly damaging	0.57
R1800:Myo15b	UTSW	11	115880509	critical splice donor site	probably null
R1843:Myo15b	UTSW	11	115869586	missense	probably benign	0.04
R1888:Myo15b	UTSW	11	115887073	missense	probably damaging	1.00
R1888:Myo15b	UTSW	11	115887073	missense	probably damaging	1.00
R1894:Myo15b	UTSW	11	115887073	missense	probably damaging	1.00
R1917:Myo15b	UTSW	11	115882254	missense	possibly damaging	0.51
R1934:Myo15b	UTSW	11	115863484	missense	probably benign	0.30
R1939:Myo15b	UTSW	11	115887703	missense	probably benign	0.00
R1945:Myo15b	UTSW	11	115878398	missense	probably damaging	1.00
R1986:Myo15b	UTSW	11	115882875	missense	probably benign	0.31
R2130:Myo15b	UTSW	11	115871643	missense	probably benign	0.08
R2138:Myo15b	UTSW	11	115883807	missense	probably benign	0.00
R2176:Myo15b	UTSW	11	115866572	missense	probably damaging	1.00
R2415:Myo15b	UTSW	11	115879564	missense	probably benign	0.00
R2483:Myo15b	UTSW	11	115864739	missense	probably benign	0.04
R3620:Myo15b	UTSW	11	115871187	missense	possibly damaging	0.46
R3716:Myo15b	UTSW	11	115863413	missense	probably benign	0.01
R4013:Myo15b	UTSW	11	115871456	nonsense	probably null
R4021:Myo15b	UTSW	11	115873505	missense	probably benign	0.07
R4119:Myo15b	UTSW	11	115873492	missense	probably benign	0.07
R4120:Myo15b	UTSW	11	115873492	missense	probably benign	0.07
R4499:Myo15b	UTSW	11	115890952	missense	probably benign	0.00
R4653:Myo15b	UTSW	11	115879987	critical splice donor site	probably null
R4655:Myo15b	UTSW	11	115890697	missense	probably damaging	1.00
R4700:Myo15b	UTSW	11	115861935	missense	possibly damaging	0.55
R4702:Myo15b	UTSW	11	115884008	missense	probably benign	0.01
R4777:Myo15b	UTSW	11	115879652	missense	probably damaging	0.99
R4833:Myo15b	UTSW	11	115887602	missense	possibly damaging	0.51
R5083:Myo15b	UTSW	11	115866656	missense	probably benign	0.01
R5121:Myo15b	UTSW	11	115886054	missense	probably damaging	1.00
R5146:Myo15b	UTSW	11	115891198	missense	probably benign	0.00
R5535:Myo15b	UTSW	11	115881301	missense	probably damaging	1.00
R5647:Myo15b	UTSW	11	115871511	missense	probably damaging	0.99
R5849:Myo15b	UTSW	11	115881933	missense	probably damaging	1.00
R5882:Myo15b	UTSW	11	115869596	missense	probably damaging	1.00
R5956:Myo15b	UTSW	11	115873757	missense	probably benign	0.34
R6273:Myo15b	UTSW	11	115862799	missense	possibly damaging	0.63
R6302:Myo15b	UTSW	11	115886239	missense	possibly damaging	0.88
R6318:Myo15b	UTSW	11	115890831	missense	probably damaging	1.00
R6462:Myo15b	UTSW	11	115859442	missense	probably benign	0.01
R6792:Myo15b	UTSW	11	115885097	missense	probably damaging	1.00
R6963:Myo15b	UTSW	11	115890714	splice site	probably null
R7015:Myo15b	UTSW	11	115871844	missense
R7020:Myo15b	UTSW	11	115866667	nonsense	probably null
R7096:Myo15b	UTSW	11	115891498	splice site	probably null
R7219:Myo15b	UTSW	11	115877095	critical splice donor site	probably null
R7400:Myo15b	UTSW	11	115860113	missense
R7413:Myo15b	UTSW	11	115878144	missense
R7483:Myo15b	UTSW	11	115858744	missense
R7523:Myo15b	UTSW	11	115890858	missense	unknown
R7737:Myo15b	UTSW	11	115887923	missense	unknown
R7784:Myo15b	UTSW	11	115861340	missense
R7921:Myo15b	UTSW	11	115887178	nonsense	probably null
R8065:Myo15b	UTSW	11	115887943	critical splice donor site	probably null
R8183:Myo15b	UTSW	11	115883017	splice site	probably null
R8193:Myo15b	UTSW	11	115885147	missense	probably damaging	1.00
R8237:Myo15b	UTSW	11	115877001	missense
R8430:Myo15b	UTSW	11	115882223	missense	probably benign	0.02
R8482:Myo15b	UTSW	11	115883257	nonsense	probably null
R8515:Myo15b	UTSW	11	115858784	missense
R8798:Myo15b	UTSW	11	115863406	missense
R8937:Myo15b	UTSW	11	115882301	missense	probably benign	0.00
R8975:Myo15b	UTSW	11	115890954	missense	unknown
R9045:Myo15b	UTSW	11	115892352	makesense	probably null
R9117:Myo15b	UTSW	11	115887917	missense	possibly damaging	0.91
R9185:Myo15b	UTSW	11	115880429	missense	unknown
R9226:Myo15b	UTSW	11	115860098	missense
R9302:Myo15b	UTSW	11	115885412	missense	possibly damaging	0.83
R9318:Myo15b	UTSW	11	115885139	missense	probably benign	0.26
R9336:Myo15b	UTSW	11	115880238	missense
R9337:Myo15b	UTSW	11	115859035	missense
R9338:Myo15b	UTSW	11	115871412	missense
R9498:Myo15b	UTSW	11	115879958	missense
R9500:Myo15b	UTSW	11	115886640	missense	probably damaging	0.98
R9602:Myo15b	UTSW	11	115878443	critical splice donor site	probably null
R9642:Myo15b	UTSW	11	115881509	missense	possibly damaging	0.67
X0020:Myo15b	UTSW	11	115871799	critical splice acceptor site	probably null
Z1176:Myo15b	UTSW	11	115883452	missense	possibly damaging	0.81
Z1176:Myo15b	UTSW	11	115887925	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CTTTCAGGTGCACAGAGAGC -3'
(R):5'- TTGGAGAAAGAGGGCTCACC -3'

Sequencing Primer
(F):5'- TGCACAGAGAGCTTTGCG -3'
(R):5'- ACCTTTCCAGGGGTGGG -3'

Posted On

2019-12-20