Incidental Mutation 'R7853:Olfr458'
ID607059
Institutional Source Beutler Lab
Gene Symbol Olfr458
Ensembl Gene ENSMUSG00000068574
Gene Nameolfactory receptor 458
SynonymsMOR257-4, GA_x6K02T2P3E9-5100053-5100994
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.065) question?
Stock #R7853 (G1)
Quality Score225.009
Status Not validated
Chromosome6
Chromosomal Location42459979-42465196 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 42460639 bp
ZygosityHeterozygous
Amino Acid Change Cysteine to Serine at position 127 (C127S)
Ref Sequence ENSEMBL: ENSMUSP00000149459 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000090156] [ENSMUST00000216650]
Predicted Effect probably damaging
Transcript: ENSMUST00000090156
AA Change: C127S

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000087617
Gene: ENSMUSG00000068574
AA Change: C127S

DomainStartEndE-ValueType
Pfam:7tm_4 31 308 8.6e-52 PFAM
Pfam:7tm_1 41 310 3.7e-25 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000216650
AA Change: C127S

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 80 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921509C19Rik A G 2: 151,473,680 I26T probably damaging Het
Aadacl4 G T 4: 144,618,022 A123S probably benign Het
Adamts20 A C 15: 94,345,990 C619G probably damaging Het
Ahcyl1 C A 3: 107,668,288 V394L probably benign Het
Ankrd17 A C 5: 90,238,966 L2378V possibly damaging Het
B3gnt3 T C 8: 71,692,713 Y337C probably damaging Het
Bcl10 A G 3: 145,924,511 K18R possibly damaging Het
Calcr G A 6: 3,707,499 A267V probably benign Het
Ces1d C T 8: 93,175,067 G425S probably benign Het
Col1a1 G T 11: 94,947,679 R899L unknown Het
Commd1 C T 11: 22,956,532 R168H possibly damaging Het
Cps1 T C 1: 67,174,481 S791P possibly damaging Het
Ctsj T A 13: 61,004,070 I58F probably damaging Het
Cyp2j5 T A 4: 96,641,419 K238N probably benign Het
Dlgap4 A G 2: 156,705,882 D423G probably benign Het
Egln1 T C 8: 124,948,517 N180D probably benign Het
Ehd1 T C 19: 6,277,195 F74S probably damaging Het
Ext1 G A 15: 53,107,485 A350V probably damaging Het
Fam186b T A 15: 99,280,747 I233F probably damaging Het
Fam193a T G 5: 34,440,129 N91K probably benign Het
Far1 T A 7: 113,554,148 N329K probably damaging Het
Fhad1 A G 4: 141,909,823 S1111P probably damaging Het
Fhl2 A G 1: 43,141,824 S69P probably damaging Het
Flg2 C A 3: 93,220,747 P2322Q unknown Het
Flvcr1 A G 1: 191,025,646 Y108H probably damaging Het
Fos T A 12: 85,476,018 S235T probably benign Het
Foxf1 T A 8: 121,084,699 S101T probably damaging Het
Gfer C A 17: 24,694,285 D198Y probably damaging Het
Ggps1 T C 13: 14,054,449 I50V probably benign Het
Gkn2 A G 6: 87,378,273 T155A probably benign Het
Gm49333 T A 16: 20,644,260 probably null Het
Gna12 C T 5: 140,760,694 C332Y probably damaging Het
H2-Ke6 T G 17: 34,027,437 D117A probably benign Het
Hc T A 2: 35,010,033 Y1096F probably damaging Het
Hoxa3 G A 6: 52,170,287 probably benign Het
Ifi206 A T 1: 173,471,534 Y835* probably null Het
Ighv13-2 T G 12: 114,357,924 E65A probably damaging Het
Kif5a A T 10: 127,235,668 Y770* probably null Het
Lfng A G 5: 140,607,629 S72G probably benign Het
Lipo2 C T 19: 33,759,944 probably benign Het
Lnpep C T 17: 17,562,847 S564N probably benign Het
Mfhas1 T A 8: 35,589,871 L500* probably null Het
Mllt6 G A 11: 97,670,316 V277I probably benign Het
Mroh5 C T 15: 73,791,340 D192N probably benign Het
Myo1f T A 17: 33,576,698 V106D probably damaging Het
Mypn T A 10: 63,145,873 I643F probably benign Het
Nup188 A G 2: 30,323,563 N669D possibly damaging Het
Olfr126 C G 17: 37,850,823 T77R probably damaging Het
Olfr154 T C 2: 85,664,345 T30A probably benign Het
Olfr281 T C 15: 98,456,985 V225A probably benign Het
Osbpl10 A G 9: 115,207,658 T241A probably damaging Het
Peg3 C T 7: 6,708,840 E1128K possibly damaging Het
Plch1 C A 3: 63,773,647 M186I probably benign Het
Pon3 A C 6: 5,236,911 L152R probably damaging Het
Prr36 G A 8: 4,213,905 P587L unknown Het
Psma2 T A 13: 14,625,247 I192N probably damaging Het
Rbm11 T C 16: 75,593,035 F30L probably damaging Het
Rpap3 G A 15: 97,678,418 A622V possibly damaging Het
Scgb2b11 T A 7: 32,209,382 N98Y probably damaging Het
Siglec1 A G 2: 131,081,292 L511P probably damaging Het
Sirpb1c C T 3: 15,832,992 V228M probably damaging Het
Smpd4 T A 16: 17,642,741 Y804N probably damaging Het
Sult6b2 T C 6: 142,801,798 D75G not run Het
Sycp3 A C 10: 88,466,506 K119N probably damaging Het
Syne2 T C 12: 76,031,504 L4703P probably damaging Het
Tex12 C G 9: 50,559,223 L20F possibly damaging Het
Them5 T A 3: 94,343,296 Y55* probably null Het
Tmem43 A T 6: 91,481,986 D213V probably benign Het
Tnfrsf10b A C 14: 69,767,790 Q44P unknown Het
Trbv2 A T 6: 41,047,902 Q84L probably benign Het
Trim2 C T 3: 84,305,230 probably benign Het
Trim36 A G 18: 46,172,491 V475A probably benign Het
Ttbk1 C A 17: 46,447,343 E788D probably benign Het
Ttn T C 2: 76,713,282 D33120G probably damaging Het
Ttn T A 2: 76,745,912 N24879I probably damaging Het
Ube4a T A 9: 44,953,010 Q76L probably benign Het
Vmn2r27 T A 6: 124,192,021 I717F probably damaging Het
Vmn2r45 T C 7: 8,482,988 K434E possibly damaging Het
Vta1 T C 10: 14,655,717 T305A probably damaging Het
Zfp354c TCACACTCGGCACA TCACA 11: 50,815,240 probably benign Het
Other mutations in Olfr458
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00915:Olfr458 APN 6 42460950 missense probably benign
IGL00983:Olfr458 APN 6 42460095 missense probably benign
IGL01655:Olfr458 APN 6 42460540 missense probably benign 0.41
IGL02017:Olfr458 APN 6 42460824 missense probably benign 0.40
IGL02420:Olfr458 APN 6 42460176 missense probably benign 0.03
IGL03145:Olfr458 APN 6 42460500 missense probably benign 0.05
IGL03171:Olfr458 APN 6 42460530 missense possibly damaging 0.89
IGL03333:Olfr458 APN 6 42460839 missense probably damaging 1.00
R1768:Olfr458 UTSW 6 42460677 missense probably damaging 1.00
R1908:Olfr458 UTSW 6 42460426 missense probably benign 0.15
R2198:Olfr458 UTSW 6 42461016 start codon destroyed probably null 1.00
R2336:Olfr458 UTSW 6 42460729 missense probably damaging 1.00
R2512:Olfr458 UTSW 6 42460273 missense probably damaging 0.99
R3433:Olfr458 UTSW 6 42460954 missense probably benign
R5338:Olfr458 UTSW 6 42460974 missense probably benign 0.11
R5341:Olfr458 UTSW 6 42460164 missense probably damaging 1.00
R5498:Olfr458 UTSW 6 42460294 missense probably benign 0.11
R6558:Olfr458 UTSW 6 42460777 missense probably benign 0.02
R6594:Olfr458 UTSW 6 42460375 missense probably benign 0.01
R7107:Olfr458 UTSW 6 42460554 missense possibly damaging 0.78
R8050:Olfr458 UTSW 6 42460830 missense probably damaging 1.00
R8684:Olfr458 UTSW 6 42460893 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GAAGCCAGTATCACCACTTGG -3'
(R):5'- TGGACATCTGCTACACCTCC -3'

Sequencing Primer
(F):5'- AGTCTGACCAGTGCCAGTGTC -3'
(R):5'- ACCTCCAGTGGGGTTCC -3'
Posted On2019-12-20