Mutagenetix > Incidental Mutation

Incidental Mutation 'R7855:Zfp354c'

607195

Institutional Source

Beutler Lab

Gene Symbol

Zfp354c

Ensembl Gene

ENSMUSG00000044807

Gene Name

zinc finger protein 354C

Synonyms

AJ18, Kid3, 5330421P20Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R7855 (G1)

Quality Score

217.468

Status

Not validated

Chromosome

Chromosomal Location

50811086-50827724 bp(-) (GRCm38)

Type of Mutation

small deletion (3 aa in frame mutation)

DNA Base Change (assembly)

TCACACTCGGCACA to TCACA at 50815240 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000104763 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000632] [ENSMUST00000109135]

AlphaFold

Q571J5

Predicted Effect

probably benign
Transcript: ENSMUST00000000632

SMART Domains

Protein: ENSMUSP00000000632
Gene: ENSMUSG00000044807

Domain	Start	End	E-Value	Type
KRAB	14	74	9.98e-33	SMART
ZnF_C2H2	218	240	5.9e-3	SMART
ZnF_C2H2	246	268	3.74e-5	SMART
ZnF_C2H2	274	296	1.72e-4	SMART
ZnF_C2H2	302	324	7.78e-3	SMART
ZnF_C2H2	330	352	1.92e-2	SMART
ZnF_C2H2	358	380	4.79e-3	SMART
ZnF_C2H2	386	408	1.1e-2	SMART
ZnF_C2H2	414	436	5.67e-5	SMART
ZnF_C2H2	442	464	9.08e-4	SMART
ZnF_C2H2	470	492	5.59e-4	SMART
ZnF_C2H2	498	520	3.39e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109135

SMART Domains

Protein: ENSMUSP00000104763
Gene: ENSMUSG00000044807

Domain	Start	End	E-Value	Type
KRAB	14	74	9.98e-33	SMART
ZnF_C2H2	218	240	5.9e-3	SMART
ZnF_C2H2	246	268	3.74e-5	SMART
ZnF_C2H2	274	296	1.72e-4	SMART
ZnF_C2H2	302	324	7.78e-3	SMART
ZnF_C2H2	330	352	1.92e-2	SMART
ZnF_C2H2	358	380	4.79e-3	SMART
ZnF_C2H2	386	408	1.1e-2	SMART
ZnF_C2H2	414	436	5.67e-5	SMART
ZnF_C2H2	442	464	9.08e-4	SMART
ZnF_C2H2	470	492	5.59e-4	SMART
ZnF_C2H2	498	520	3.39e-3	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700081O15Rik	T	A	19: 7,422,256	I456N	probably damaging	Het
Abca6	A	T	11: 110,191,628	V1173D	probably benign	Het
Ace	A	T	11: 105,972,379	M327L	probably benign	Het
Bcl2l2	C	T	14: 54,884,379		probably benign	Het
Bicdl2	C	T	17: 23,666,017	Q231*	probably null	Het
Brms1l	A	T	12: 55,866,053	D277V	possibly damaging	Het
Cd38	C	A	5: 43,901,448	L135M	probably damaging	Het
Col6a3	G	T	1: 90,810,621	P1059T	possibly damaging	Het
Coro1c	A	T	5: 113,848,597	M262K	probably benign	Het
Cpxm2	G	T	7: 132,057,695	P481Q	possibly damaging	Het
Dnah12	G	T	14: 26,829,329	V2543F	probably benign	Het
Dock2	A	C	11: 34,273,698	D1145E	probably damaging	Het
Elf3	G	A	1: 135,254,352	R364W	probably damaging	Het
Eln	AGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCAGGGACACCAGC	AGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCAGGGACACCAGC	5: 134,711,081		probably benign	Het
Epha3	A	G	16: 63,773,560	I55T	probably damaging	Het
Fam69c	G	A	18: 84,730,046		probably benign	Het
Gfer	C	A	17: 24,694,285	D198Y	probably damaging	Het
Gm10436	A	T	12: 88,176,083	I450N	probably benign	Het
Gm11568	A	T	11: 99,858,184	T72S	unknown	Het
Gm21994	C	T	2: 150,255,146	R121Q	probably benign	Het
Gm5415	A	C	1: 32,546,033	I265M	probably damaging	Het
Igkv3-1	C	T	6: 70,704,069	A84V	probably benign	Het
Il1rl2	A	C	1: 40,343,119	Y197S	probably damaging	Het
Il2ra	T	C	2: 11,680,336	I161T	possibly damaging	Het
Itgb8	C	T	12: 119,166,772	R667H	probably benign	Het
Kcnh7	G	A	2: 62,837,194	Q334*	probably null	Het
Lctl	A	C	9: 64,133,216	R480S	possibly damaging	Het
Lrba	T	G	3: 86,315,430	I617S	possibly damaging	Het
Marf1	G	T	16: 14,114,201	H1651N	probably benign	Het
Mitf	T	A	6: 97,993,196	Y142N	probably damaging	Het
Olfr569	A	G	7: 102,887,628	V175A	probably benign	Het
Olfr620	T	A	7: 103,611,772	I194F	possibly damaging	Het
Pecam1	A	G	11: 106,671,750	V708A	probably benign	Het
Pinlyp	C	T	7: 24,542,440		probably null	Het
Polh	G	A	17: 46,175,248	R382W	probably damaging	Het
Prdm10	A	G	9: 31,327,474	I221V	probably benign	Het
Pskh1	G	T	8: 105,913,090	R134L	probably benign	Het
Ptpre	A	G	7: 135,651,995	N6D	probably benign	Het
Rasgrp4	C	T	7: 29,150,610	P58L	unknown	Het
Rhbdf2	G	T	11: 116,602,240	C393*	probably null	Het
Rlf	T	C	4: 121,182,691	I174M	possibly damaging	Het
Ryr2	T	A	13: 11,706,623	R2641*	probably null	Het
Simc1	A	G	13: 54,524,832	H331R	probably benign	Het
Skp2	A	G	15: 9,122,241	S256P	probably benign	Het
Smarcd2	T	C	11: 106,267,566	R10G	probably benign	Het
Spef2	A	G	15: 9,687,895	L480P	possibly damaging	Het
Tenm4	A	G	7: 96,873,874	H1541R	probably damaging	Het
Top1	T	A	2: 160,714,088	L489Q	probably damaging	Het
Ttll13	C	T	7: 80,254,097	H258Y	probably damaging	Het
Unc80	A	G	1: 66,483,349	R237G	possibly damaging	Het
Vmn1r55	A	G	7: 5,146,624	F267L	probably benign	Het
Vmn2r96	T	A	17: 18,597,868	M761K	possibly damaging	Het
Vps33a	G	A	5: 123,570,979	H58Y	possibly damaging	Het
Zfp467	T	C	6: 48,439,181	Q179R	probably damaging	Het
Zfp729a	A	T	13: 67,619,948	S721T	possibly damaging	Het

Other mutations in Zfp354c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00923:Zfp354c	APN	11	50815613	missense	probably damaging	0.97
IGL01615:Zfp354c	APN	11	50817905	missense	possibly damaging	0.90
IGL03019:Zfp354c	APN	11	50817194	missense	probably damaging	1.00
R0546:Zfp354c	UTSW	11	50815630	missense	probably benign	0.12
R1370:Zfp354c	UTSW	11	50815840	missense	probably benign
R2109:Zfp354c	UTSW	11	50817142	missense	probably benign	0.01
R2850:Zfp354c	UTSW	11	50815331	nonsense	probably null
R4010:Zfp354c	UTSW	11	50814944	missense	probably damaging	0.98
R5034:Zfp354c	UTSW	11	50815039	missense	probably benign	0.14
R5430:Zfp354c	UTSW	11	50815195	missense	probably benign	0.02
R5439:Zfp354c	UTSW	11	50815770	missense	probably benign	0.01
R5905:Zfp354c	UTSW	11	50815426	missense	probably damaging	1.00
R6244:Zfp354c	UTSW	11	50814971	missense	probably benign	0.41
R6264:Zfp354c	UTSW	11	50815447	missense	probably benign	0.00
R6591:Zfp354c	UTSW	11	50814775	missense	probably benign	0.41
R6650:Zfp354c	UTSW	11	50814691	missense	probably damaging	1.00
R6691:Zfp354c	UTSW	11	50814775	missense	probably benign	0.41
R7087:Zfp354c	UTSW	11	50815213	missense	probably damaging	1.00
R7313:Zfp354c	UTSW	11	50814656	missense	probably damaging	1.00
R7467:Zfp354c	UTSW	11	50815426	missense	probably damaging	1.00
R7619:Zfp354c	UTSW	11	50817808	critical splice donor site	probably null
R7699:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7710:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7712:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7747:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7748:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7784:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7816:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7817:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7853:Zfp354c	UTSW	11	50815240	small deletion	probably benign
R7870:Zfp354c	UTSW	11	50815238	small deletion	probably benign
R8852:Zfp354c	UTSW	11	50815192	missense	probably damaging	1.00
R8860:Zfp354c	UTSW	11	50815192	missense	probably damaging	1.00
R8862:Zfp354c	UTSW	11	50817891	missense	probably benign	0.15
R9169:Zfp354c	UTSW	11	50815261	missense	probably damaging	1.00
R9215:Zfp354c	UTSW	11	50815839	missense	probably benign	0.31
R9273:Zfp354c	UTSW	11	50815232	missense	probably damaging	1.00
R9762:Zfp354c	UTSW	11	50815412	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTAAAGGTTCTCCCACATTGC -3'
(R):5'- TGCTACGTATCACACTTCAGAG -3'

Sequencing Primer
(F):5'- TTGCAAACACTGGCAAAGCTG -3'
(R):5'- TCTTCTAGAGCACAAGAGGCTTC -3'

Posted On

2019-12-20