Mutagenetix > Incidental Mutation

Incidental Mutation 'R7855:Vmn2r96'

607214

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r96

Ensembl Gene

ENSMUSG00000091679

Gene Name

vomeronasal 2, receptor 96

Synonyms

EG433070

MMRRC Submission

045908-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.183) question?

Stock #

R7855 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

18573151-18616003 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 18597868 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 761 (M761K)

Ref Sequence

ENSEMBL: ENSMUSP00000135095 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165692] [ENSMUST00000177244] [ENSMUST00000231261] [ENSMUST00000231286]

AlphaFold

E9PZU5

Predicted Effect

probably damaging
Transcript: ENSMUST00000165692
AA Change: M569K

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000131564
Gene: ENSMUSG00000091679
AA Change: M569K

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	257	8.6e-20	PFAM
Pfam:NCD3G	317	370	3.7e-22	PFAM
Pfam:7tm_3	402	638	3.9e-53	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000177244
AA Change: M761K

PolyPhen 2 Score 0.592 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000135095
Gene: ENSMUSG00000091679
AA Change: M761K

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	245	4.2e-19	PFAM
Pfam:NCD3G	317	370	6.9e-21	PFAM
Pfam:7tm_3	400	639	3.4e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231261

Predicted Effect

possibly damaging
Transcript: ENSMUST00000231286
AA Change: M761K

PolyPhen 2 Score 0.592 (Sensitivity: 0.87; Specificity: 0.91)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700081O15Rik	T	A	19: 7,422,256	I456N	probably damaging	Het
Abca6	A	T	11: 110,191,628	V1173D	probably benign	Het
Ace	A	T	11: 105,972,379	M327L	probably benign	Het
Bcl2l2	C	T	14: 54,884,379		probably benign	Het
Bicdl2	C	T	17: 23,666,017	Q231*	probably null	Het
Brms1l	A	T	12: 55,866,053	D277V	possibly damaging	Het
Cd38	C	A	5: 43,901,448	L135M	probably damaging	Het
Col6a3	G	T	1: 90,810,621	P1059T	possibly damaging	Het
Coro1c	A	T	5: 113,848,597	M262K	probably benign	Het
Cpxm2	G	T	7: 132,057,695	P481Q	possibly damaging	Het
Dnah12	G	T	14: 26,829,329	V2543F	probably benign	Het
Dock2	A	C	11: 34,273,698	D1145E	probably damaging	Het
Elf3	G	A	1: 135,254,352	R364W	probably damaging	Het
Eln	AGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCAGGGACACCAGC	AGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCGGGGACACCAGCACCAGCCCCAAATCCAGGGACACCAGC	5: 134,711,081		probably benign	Het
Epha3	A	G	16: 63,773,560	I55T	probably damaging	Het
Fam69c	G	A	18: 84,730,046		probably benign	Het
Gfer	C	A	17: 24,694,285	D198Y	probably damaging	Het
Gm10436	A	T	12: 88,176,083	I450N	probably benign	Het
Gm11568	A	T	11: 99,858,184	T72S	unknown	Het
Gm21994	C	T	2: 150,255,146	R121Q	probably benign	Het
Gm5415	A	C	1: 32,546,033	I265M	probably damaging	Het
Igkv3-1	C	T	6: 70,704,069	A84V	probably benign	Het
Il1rl2	A	C	1: 40,343,119	Y197S	probably damaging	Het
Il2ra	T	C	2: 11,680,336	I161T	possibly damaging	Het
Itgb8	C	T	12: 119,166,772	R667H	probably benign	Het
Kcnh7	G	A	2: 62,837,194	Q334*	probably null	Het
Lctl	A	C	9: 64,133,216	R480S	possibly damaging	Het
Lrba	T	G	3: 86,315,430	I617S	possibly damaging	Het
Marf1	G	T	16: 14,114,201	H1651N	probably benign	Het
Mitf	T	A	6: 97,993,196	Y142N	probably damaging	Het
Olfr569	A	G	7: 102,887,628	V175A	probably benign	Het
Olfr620	T	A	7: 103,611,772	I194F	possibly damaging	Het
Pecam1	A	G	11: 106,671,750	V708A	probably benign	Het
Pinlyp	C	T	7: 24,542,440		probably null	Het
Polh	G	A	17: 46,175,248	R382W	probably damaging	Het
Prdm10	A	G	9: 31,327,474	I221V	probably benign	Het
Pskh1	G	T	8: 105,913,090	R134L	probably benign	Het
Ptpre	A	G	7: 135,651,995	N6D	probably benign	Het
Rasgrp4	C	T	7: 29,150,610	P58L	unknown	Het
Rhbdf2	G	T	11: 116,602,240	C393*	probably null	Het
Rlf	T	C	4: 121,182,691	I174M	possibly damaging	Het
Ryr2	T	A	13: 11,706,623	R2641*	probably null	Het
Simc1	A	G	13: 54,524,832	H331R	probably benign	Het
Skp2	A	G	15: 9,122,241	S256P	probably benign	Het
Smarcd2	T	C	11: 106,267,566	R10G	probably benign	Het
Spef2	A	G	15: 9,687,895	L480P	possibly damaging	Het
Tenm4	A	G	7: 96,873,874	H1541R	probably damaging	Het
Top1	T	A	2: 160,714,088	L489Q	probably damaging	Het
Ttll13	C	T	7: 80,254,097	H258Y	probably damaging	Het
Unc80	A	G	1: 66,483,349	R237G	possibly damaging	Het
Vmn1r55	A	G	7: 5,146,624	F267L	probably benign	Het
Vps33a	G	A	5: 123,570,979	H58Y	possibly damaging	Het
Zfp354c	TCACACTCGGCACA	TCACA	11: 50,815,240		probably benign	Het
Zfp467	T	C	6: 48,439,181	Q179R	probably damaging	Het
Zfp729a	A	T	13: 67,619,948	S721T	possibly damaging	Het

Other mutations in Vmn2r96

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Vmn2r96	APN	17	18583815	missense	probably benign	0.00
IGL00563:Vmn2r96	APN	17	18583815	missense	probably benign	0.00
IGL00769:Vmn2r96	APN	17	18583819	missense	probably benign	0.00
IGL01314:Vmn2r96	APN	17	18582964	missense	probably benign	0.00
IGL01983:Vmn2r96	APN	17	18597265	missense	probably damaging	0.99
IGL02314:Vmn2r96	APN	17	18583959	missense	probably benign	0.01
IGL02600:Vmn2r96	APN	17	18597567	missense	probably benign
IGL02672:Vmn2r96	APN	17	18598114	missense	probably benign	0.02
IGL02750:Vmn2r96	APN	17	18582589	missense	probably benign	0.01
IGL03068:Vmn2r96	APN	17	18582875	missense	probably benign	0.00
IGL03411:Vmn2r96	APN	17	18586372	missense	possibly damaging	0.88
R0316:Vmn2r96	UTSW	17	18582565	missense	probably damaging	1.00
R0502:Vmn2r96	UTSW	17	18584000	missense	probably benign
R0580:Vmn2r96	UTSW	17	18582638	missense	probably damaging	1.00
R0652:Vmn2r96	UTSW	17	18597568	missense	probably benign	0.05
R0789:Vmn2r96	UTSW	17	18582476	missense	possibly damaging	0.81
R1462:Vmn2r96	UTSW	17	18597398	missense	possibly damaging	0.94
R1462:Vmn2r96	UTSW	17	18597398	missense	possibly damaging	0.94
R1660:Vmn2r96	UTSW	17	18597726	missense	probably benign	0.00
R1755:Vmn2r96	UTSW	17	18582653	missense	possibly damaging	0.50
R1843:Vmn2r96	UTSW	17	18597921	missense	probably benign	0.14
R1943:Vmn2r96	UTSW	17	18586402	missense	probably benign	0.09
R1993:Vmn2r96	UTSW	17	18583876	missense	probably damaging	1.00
R2018:Vmn2r96	UTSW	17	18584001	missense	probably benign
R2405:Vmn2r96	UTSW	17	18597840	missense	probably damaging	0.96
R3977:Vmn2r96	UTSW	17	18597679	missense	probably damaging	1.00
R3979:Vmn2r96	UTSW	17	18597679	missense	probably damaging	1.00
R4059:Vmn2r96	UTSW	17	18598077	missense	probably benign	0.20
R4693:Vmn2r96	UTSW	17	18583008	missense	probably benign	0.03
R4709:Vmn2r96	UTSW	17	18582826	missense	probably benign	0.36
R4776:Vmn2r96	UTSW	17	18597508	missense	probably damaging	1.00
R4882:Vmn2r96	UTSW	17	18597604	missense	probably damaging	1.00
R4920:Vmn2r96	UTSW	17	18582656	missense	probably benign	0.07
R5143:Vmn2r96	UTSW	17	18583858	missense	possibly damaging	0.65
R5301:Vmn2r96	UTSW	17	18597688	missense	probably damaging	0.98
R5507:Vmn2r96	UTSW	17	18597829	missense	probably damaging	1.00
R6151:Vmn2r96	UTSW	17	18583959	missense	probably benign	0.01
R6181:Vmn2r96	UTSW	17	18583864	missense	probably benign
R6339:Vmn2r96	UTSW	17	18583862	missense	possibly damaging	0.84
R6404:Vmn2r96	UTSW	17	18597531	missense	probably damaging	1.00
R6452:Vmn2r96	UTSW	17	18583855	missense	probably benign	0.07
R6749:Vmn2r96	UTSW	17	18598090	missense	probably damaging	0.99
R6813:Vmn2r96	UTSW	17	18581854	missense	probably benign	0.04
R6851:Vmn2r96	UTSW	17	18582538	missense	possibly damaging	0.95
R6944:Vmn2r96	UTSW	17	18597629	missense	probably benign	0.16
R6949:Vmn2r96	UTSW	17	18597838	missense	probably damaging	1.00
R6962:Vmn2r96	UTSW	17	18598021	missense	probably damaging	0.99
R6990:Vmn2r96	UTSW	17	18583820	missense	probably benign
R7149:Vmn2r96	UTSW	17	18597727	missense	possibly damaging	0.89
R7346:Vmn2r96	UTSW	17	18582767	missense	probably benign	0.15
R7385:Vmn2r96	UTSW	17	18583040	missense	probably damaging	1.00
R7442:Vmn2r96	UTSW	17	18573400	missense	probably benign	0.02
R7509:Vmn2r96	UTSW	17	18582733	missense	probably benign	0.02
R7652:Vmn2r96	UTSW	17	18573570	missense	probably benign
R7659:Vmn2r96	UTSW	17	18573487	missense	probably benign	0.00
R7753:Vmn2r96	UTSW	17	18586401	missense	possibly damaging	0.46
R8166:Vmn2r96	UTSW	17	18582482	missense	probably damaging	1.00
R8260:Vmn2r96	UTSW	17	18583981	missense	probably benign	0.04
R8323:Vmn2r96	UTSW	17	18582761	missense	probably damaging	0.99
R8787:Vmn2r96	UTSW	17	18597988	missense	probably damaging	1.00
R8837:Vmn2r96	UTSW	17	18582626	missense	probably benign
R8933:Vmn2r96	UTSW	17	18583979	missense	probably benign	0.11
R9306:Vmn2r96	UTSW	17	18582964	missense	probably benign	0.00
R9481:Vmn2r96	UTSW	17	18573359	start gained	probably benign
R9626:Vmn2r96	UTSW	17	18573496	missense	probably benign	0.14
R9629:Vmn2r96	UTSW	17	18582995	missense	probably benign	0.15
Z1088:Vmn2r96	UTSW	17	18597366	missense	possibly damaging	0.86
Z1177:Vmn2r96	UTSW	17	18598114	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GGGTCCGAACTACATCATTCC -3'
(R):5'- CTTGGGACCGAATATGAAGGC -3'

Sequencing Primer
(F):5'- GGTCCGAACTACATCATTCCTATCTG -3'
(R):5'- CAAGGAGTGCTGTGCTGGAC -3'

Posted On

2019-12-20