Mutagenetix > Incidental Mutations

Incidental Mutation 'R7861:Eif4g1'

607546

Institutional Source

Beutler Lab

Gene Symbol

Eif4g1

Ensembl Gene

ENSMUSG00000045983

Gene Name

eukaryotic translation initiation factor 4, gamma 1

Synonyms

E030015G23Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably essential (E-score: 0.970) question?

Stock #

R7861 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20668313-20692884 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 20679702 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 403 (V403E)

Ref Sequence

ENSEMBL: ENSMUSP00000047678 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044783] [ENSMUST00000073840] [ENSMUST00000115457] [ENSMUST00000115460] [ENSMUST00000115461] [ENSMUST00000115463] [ENSMUST00000128594] [ENSMUST00000128840] [ENSMUST00000136713] [ENSMUST00000140576] [ENSMUST00000141034] [ENSMUST00000142344] [ENSMUST00000143939] [ENSMUST00000150333] [ENSMUST00000151679] [ENSMUST00000154594] [ENSMUST00000154950] [ENSMUST00000156226] [ENSMUST00000231618]

Predicted Effect

probably benign
Transcript: ENSMUST00000044783
AA Change: V403E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000047678
Gene: ENSMUSG00000045983
AA Change: V403E

Domain	Start	End	E-Value	Type
low complexity region	60	81	N/A	INTRINSIC
PDB:1LJ2\|D	179	206	1e-10	PDB
low complexity region	260	286	N/A	INTRINSIC
low complexity region	436	457	N/A	INTRINSIC
low complexity region	464	487	N/A	INTRINSIC
Blast:MIF4G	638	683	7e-9	BLAST
low complexity region	685	707	N/A	INTRINSIC
MIF4G	765	993	5.14e-72	SMART
low complexity region	1035	1047	N/A	INTRINSIC
low complexity region	1092	1106	N/A	INTRINSIC
low complexity region	1157	1178	N/A	INTRINSIC
low complexity region	1186	1201	N/A	INTRINSIC
MA3	1242	1354	3.83e-39	SMART
low complexity region	1441	1452	N/A	INTRINSIC
eIF5C	1508	1595	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000073840
AA Change: V396E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000073506
Gene: ENSMUSG00000045983
AA Change: V396E

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	1e-10	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	676	7e-9	BLAST
low complexity region	678	700	N/A	INTRINSIC
MIF4G	758	986	5.14e-72	SMART
low complexity region	1028	1040	N/A	INTRINSIC
low complexity region	1085	1099	N/A	INTRINSIC
low complexity region	1150	1171	N/A	INTRINSIC
low complexity region	1179	1194	N/A	INTRINSIC
MA3	1235	1347	3.83e-39	SMART
low complexity region	1434	1445	N/A	INTRINSIC
eIF5C	1501	1588	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115457
AA Change: V356E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000111117
Gene: ENSMUSG00000045983
AA Change: V356E

Domain	Start	End	E-Value	Type
low complexity region	13	34	N/A	INTRINSIC
PDB:1LJ2\|D	132	159	9e-11	PDB
low complexity region	213	239	N/A	INTRINSIC
low complexity region	389	410	N/A	INTRINSIC
low complexity region	417	440	N/A	INTRINSIC
Blast:MIF4G	591	636	7e-9	BLAST
low complexity region	638	660	N/A	INTRINSIC
MIF4G	718	946	5.14e-72	SMART
low complexity region	988	1000	N/A	INTRINSIC
low complexity region	1045	1059	N/A	INTRINSIC
low complexity region	1110	1131	N/A	INTRINSIC
low complexity region	1139	1154	N/A	INTRINSIC
MA3	1195	1307	3.83e-39	SMART
low complexity region	1394	1405	N/A	INTRINSIC
eIF5C	1461	1548	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115460
AA Change: V403E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000111120
Gene: ENSMUSG00000045983
AA Change: V403E

Domain	Start	End	E-Value	Type
low complexity region	60	81	N/A	INTRINSIC
PDB:1LJ2\|D	179	206	1e-10	PDB
low complexity region	260	286	N/A	INTRINSIC
low complexity region	436	457	N/A	INTRINSIC
low complexity region	464	487	N/A	INTRINSIC
Blast:MIF4G	638	683	7e-9	BLAST
low complexity region	685	707	N/A	INTRINSIC
MIF4G	765	993	5.14e-72	SMART
low complexity region	1035	1047	N/A	INTRINSIC
low complexity region	1092	1106	N/A	INTRINSIC
low complexity region	1157	1178	N/A	INTRINSIC
low complexity region	1186	1201	N/A	INTRINSIC
MA3	1242	1354	3.83e-39	SMART
low complexity region	1441	1452	N/A	INTRINSIC
eIF5C	1508	1595	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115461
AA Change: V396E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000111121
Gene: ENSMUSG00000045983
AA Change: V396E

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	1e-10	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	676	8e-9	BLAST
low complexity region	678	693	N/A	INTRINSIC
MIF4G	759	987	5.14e-72	SMART
low complexity region	1029	1041	N/A	INTRINSIC
low complexity region	1086	1100	N/A	INTRINSIC
low complexity region	1151	1172	N/A	INTRINSIC
low complexity region	1180	1195	N/A	INTRINSIC
MA3	1236	1348	3.83e-39	SMART
low complexity region	1435	1446	N/A	INTRINSIC
eIF5C	1502	1589	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115463
AA Change: V396E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000111123
Gene: ENSMUSG00000045983
AA Change: V396E

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	1e-10	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	676	7e-9	BLAST
low complexity region	678	700	N/A	INTRINSIC
MIF4G	758	986	5.14e-72	SMART
low complexity region	1030	1036	N/A	INTRINSIC
low complexity region	1078	1092	N/A	INTRINSIC
low complexity region	1143	1164	N/A	INTRINSIC
low complexity region	1172	1187	N/A	INTRINSIC
MA3	1228	1340	3.83e-39	SMART
low complexity region	1427	1438	N/A	INTRINSIC
eIF5C	1494	1581	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000128594
AA Change: V232E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000144594
Gene: ENSMUSG00000045983
AA Change: V232E

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	8	35	5e-11	PDB
low complexity region	89	115	N/A	INTRINSIC
low complexity region	265	286	N/A	INTRINSIC
low complexity region	293	316	N/A	INTRINSIC
Blast:MIF4G	467	512	4e-9	BLAST
low complexity region	514	536	N/A	INTRINSIC
MIF4G	594	795	1.1e-52	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000128840

SMART Domains

Protein: ENSMUSP00000143861
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	85	112	5e-13	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000136713

SMART Domains

Protein: ENSMUSP00000143999
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	85	112	3e-13	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000140576

SMART Domains

Protein: ENSMUSP00000117587
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	20	41	N/A	INTRINSIC
PDB:1LJ2\|D	129	156	3e-12	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000141034

SMART Domains

Protein: ENSMUSP00000120035
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	60	81	N/A	INTRINSIC
PDB:4F02\|F	175	200	4e-11	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000142344
AA Change: V396E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000116029
Gene: ENSMUSG00000045983
AA Change: V396E

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	5e-11	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	672	6e-8	BLAST
low complexity region	678	693	N/A	INTRINSIC
MIF4G	759	958	5.49e-49	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143939
AA Change: V106E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000144320
Gene: ENSMUSG00000045983
AA Change: V106E

Domain	Start	End	E-Value	Type
low complexity region	139	160	N/A	INTRINSIC
low complexity region	167	190	N/A	INTRINSIC
Blast:MIF4G	341	386	6e-9	BLAST
low complexity region	388	410	N/A	INTRINSIC
MIF4G	468	696	2.2e-74	SMART
low complexity region	738	750	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
low complexity region	860	881	N/A	INTRINSIC
low complexity region	889	904	N/A	INTRINSIC
MA3	945	1057	1.7e-41	SMART
low complexity region	1144	1155	N/A	INTRINSIC
eIF5C	1211	1298	1.8e-35	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000150333
AA Change: V337E

PolyPhen 2 Score 0.921 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000144107
Gene: ENSMUSG00000045983
AA Change: V337E

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	113	140	5e-11	PDB
low complexity region	194	220	N/A	INTRINSIC
low complexity region	370	391	N/A	INTRINSIC
low complexity region	398	421	N/A	INTRINSIC
Blast:MIF4G	572	613	9e-8	BLAST
low complexity region	619	641	N/A	INTRINSIC
MIF4G	699	900	1.1e-52	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000151679

SMART Domains

Protein: ENSMUSP00000120698
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	13	34	N/A	INTRINSIC
PDB:1LJ2\|D	132	159	8e-13	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000154594

SMART Domains

Protein: ENSMUSP00000144233
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	8	35	3e-12	PDB
low complexity region	89	115	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000154950

SMART Domains

Protein: ENSMUSP00000115230
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	60	81	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000156226

SMART Domains

Protein: ENSMUSP00000119215
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000231598

Predicted Effect

probably benign
Transcript: ENSMUST00000231618

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]
PHENOTYPE: Mice homozygous for an amino acid substitution (R1207H) are viable and fertile. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700016H13Rik	T	A	5: 103,649,494	I50F	possibly damaging	Het
Abcc3	T	C	11: 94,357,249	D1175G	probably null	Het
Accs	C	A	2: 93,835,732	*503L	probably null	Het
Adgra2	A	G	8: 27,114,457	E520G	probably damaging	Het
Aldh7a1	C	A	18: 56,548,453	C215F	probably benign	Het
Apbb1ip	A	C	2: 22,816,978	D9A	unknown	Het
Atad2	G	A	15: 58,125,780	A228V	probably benign	Het
Atp10a	T	C	7: 58,788,359	S430P	probably damaging	Het
Atp1a3	T	C	7: 25,001,148	D6G	unknown	Het
Brca1	A	T	11: 101,526,422	N295K	possibly damaging	Het
Caly	C	A	7: 140,081,388		probably benign	Het
Ces1h	A	G	8: 93,357,425	Y386H	unknown	Het
Col14a1	A	G	15: 55,444,616	D1044G	unknown	Het
Csf2rb	A	G	15: 78,349,157	D888G	probably damaging	Het
Cux1	T	C	5: 136,252,604	E568G	possibly damaging	Het
Cyhr1	C	T	15: 76,648,186	D241N	probably benign	Het
Dhrs7b	T	A	11: 60,855,742	L219Q	probably damaging	Het
Dlg5	G	A	14: 24,245,212	P80L	probably damaging	Het
Dnah14	C	T	1: 181,616,759	P545S	probably damaging	Het
Dnajc24	A	T	2: 106,002,035	M1K	probably null	Het
Dusp12	C	T	1: 170,874,526	W301*	probably null	Het
Dyrk1a	G	T	16: 94,691,716	G603*	probably null	Het
Epn3	T	C	11: 94,496,274	E90G	probably damaging	Het
Etl4	A	G	2: 20,805,910	S1303G	probably benign	Het
Evpl	A	T	11: 116,228,069	Y627N	probably damaging	Het
Fbxw25	A	T	9: 109,664,557	L22*	probably null	Het
Fcamr	T	A	1: 130,814,638	N587K	probably benign	Het
Fgd6	A	G	10: 94,103,331	N946S	probably benign	Het
Fndc3b	A	T	3: 27,468,999	I477N	possibly damaging	Het
Grifin	C	T	5: 140,564,525	A54T	probably benign	Het
Gtf2b	A	G	3: 142,781,344	I180M	probably damaging	Het
Invs	A	T	4: 48,397,559	D378V	possibly damaging	Het
Itgb6	T	C	2: 60,628,444	E378G	probably damaging	Het
Khdc1a	T	C	1: 21,350,399	I81T	possibly damaging	Het
Kif20b	T	A	19: 34,939,922	D617E	probably damaging	Het
Kifc3	T	A	8: 95,107,537		probably null	Het
Kirrel3	C	T	9: 35,020,123	H403Y	possibly damaging	Het
Klf15	G	A	6: 90,466,838	V132I	probably benign	Het
Kmt2a	A	G	9: 44,818,734	S3429P	unknown	Het
Lama1	T	G	17: 67,809,221	L2361R		Het
Lrp1b	T	C	2: 40,697,558	D3895G		Het
Mcoln3	A	C	3: 146,124,791	E92A	possibly damaging	Het
Myo3b	T	C	2: 70,108,688	M135T	probably damaging	Het
Mysm1	A	G	4: 94,946,967	*820Q	probably null	Het
Ncoa7	A	G	10: 30,691,060	S541P	probably benign	Het
Nup54	T	C	5: 92,431,093	T33A	unknown	Het
Olfr118	C	A	17: 37,672,517	Q165K	possibly damaging	Het
Olfr1290	T	A	2: 111,490,024	I45F	probably damaging	Het
Olfr1496	T	C	19: 13,781,446	V276A	possibly damaging	Het
Olfr45	T	G	7: 140,691,571	I222S	probably damaging	Het
Olfr586	T	A	7: 103,122,692	I27F	probably benign	Het
Otud6b	A	G	4: 14,826,414	C18R	probably benign	Het
Pde4d	A	G	13: 109,935,324	E284G	probably damaging	Het
Peg10	CATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAGGATC	CATC	6: 4,756,431		probably benign	Het
Pidd1	C	A	7: 141,440,142	W598L	probably damaging	Het
Pira2	A	T	7: 3,844,544	C49S	probably damaging	Het
Pramef6	T	C	4: 143,897,718	M70V	possibly damaging	Het
Prdx1	T	G	4: 116,693,738	D135E	probably benign	Het
Rab15	T	A	12: 76,803,129	Y88F	probably damaging	Het
Rem2	C	A	14: 54,477,799	H144Q	probably damaging	Het
Sel1l3	T	C	5: 53,144,064	D737G	probably damaging	Het
Srd5a3	C	T	5: 76,147,819	Q119*	probably null	Het
Suclg2	G	T	6: 95,594,722	Q120K	probably benign	Het
Tacc2	T	A	7: 130,625,431	M1282K	probably benign	Het
Tbc1d5	T	A	17: 50,756,692	Q620L	probably damaging	Het
Tdrd3	G	T	14: 87,472,154	A91S	probably damaging	Het
Thsd7b	T	A	1: 130,159,698	F1184Y	probably benign	Het
Trim28	T	A	7: 13,028,412	V321E	possibly damaging	Het
Trim5	C	A	7: 104,266,468		probably null	Het
Ugt2b37	A	T	5: 87,242,440	Y382*	probably null	Het
Usp40	C	T	1: 87,982,130	G534D	probably damaging	Het
Usp53	A	T	3: 122,934,463	H823Q	probably benign	Het
Vmn2r12	T	A	5: 109,087,963	M508L	probably benign	Het
Vmn2r56	T	A	7: 12,715,424	I296F	probably benign	Het
Vps13a	T	C	19: 16,655,304	S2563G	probably damaging	Het
Wdr59	A	G	8: 111,494,280	F207L		Het
Zan	A	G	5: 137,407,033	S3777P	unknown	Het
Zfp277	T	C	12: 40,315,881	N530D	possibly damaging	Het
Zfp365	G	A	10: 67,909,919	R10W	probably damaging	Het
Zfp384	A	T	6: 125,036,325	H452L	probably damaging	Het
Zfyve28	A	T	5: 34,217,143	L509Q	probably damaging	Het

Other mutations in Eif4g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00584:Eif4g1	APN	16	20686754	intron	probably benign
IGL00707:Eif4g1	APN	16	20689014	missense	probably damaging	1.00
IGL00950:Eif4g1	APN	16	20683628	missense	probably damaging	1.00
IGL01397:Eif4g1	APN	16	20679675	missense	probably damaging	0.98
IGL01657:Eif4g1	APN	16	20682216	missense	possibly damaging	0.94
IGL01875:Eif4g1	APN	16	20681040	missense	probably damaging	0.96
IGL02728:Eif4g1	APN	16	20686752	intron	probably benign
IGL03155:Eif4g1	APN	16	20692417	missense	probably damaging	1.00
IGL03339:Eif4g1	APN	16	20680984	missense	possibly damaging	0.72
R0032:Eif4g1	UTSW	16	20685898	missense	probably damaging	1.00
R0032:Eif4g1	UTSW	16	20685898	missense	probably damaging	1.00
R0138:Eif4g1	UTSW	16	20675345	missense	probably damaging	0.99
R0556:Eif4g1	UTSW	16	20675794	missense	probably damaging	0.99
R0576:Eif4g1	UTSW	16	20684068	missense	probably damaging	0.98
R1424:Eif4g1	UTSW	16	20678942	missense	probably benign	0.03
R1469:Eif4g1	UTSW	16	20680008	missense	possibly damaging	0.86
R1469:Eif4g1	UTSW	16	20680008	missense	possibly damaging	0.86
R1487:Eif4g1	UTSW	16	20678873	unclassified	probably benign
R1659:Eif4g1	UTSW	16	20681061	missense	probably damaging	0.99
R1697:Eif4g1	UTSW	16	20679780	missense	probably damaging	0.99
R1848:Eif4g1	UTSW	16	20681867	missense	probably damaging	1.00
R1855:Eif4g1	UTSW	16	20687161	missense	possibly damaging	0.77
R1865:Eif4g1	UTSW	16	20678648	missense	probably damaging	0.99
R3001:Eif4g1	UTSW	16	20692384	missense	probably damaging	1.00
R3002:Eif4g1	UTSW	16	20692384	missense	probably damaging	1.00
R4402:Eif4g1	UTSW	16	20678843	unclassified	probably benign
R4477:Eif4g1	UTSW	16	20678843	unclassified	probably benign
R4478:Eif4g1	UTSW	16	20678843	unclassified	probably benign
R4479:Eif4g1	UTSW	16	20678843	unclassified	probably benign
R4480:Eif4g1	UTSW	16	20678843	unclassified	probably benign
R4623:Eif4g1	UTSW	16	20681345	unclassified	probably benign
R4658:Eif4g1	UTSW	16	20685934	missense	possibly damaging	0.78
R4751:Eif4g1	UTSW	16	20686515	missense	possibly damaging	0.89
R4859:Eif4g1	UTSW	16	20682173	missense	probably benign	0.44
R5267:Eif4g1	UTSW	16	20685533	missense	probably damaging	0.99
R5376:Eif4g1	UTSW	16	20683827	missense	probably damaging	1.00
R5560:Eif4g1	UTSW	16	20686895	missense	probably benign
R5719:Eif4g1	UTSW	16	20689011	missense	probably damaging	1.00
R6632:Eif4g1	UTSW	16	20685520	missense	probably damaging	0.99
R6849:Eif4g1	UTSW	16	20680745	missense	probably benign	0.08
R7134:Eif4g1	UTSW	16	20681502	missense	probably damaging	1.00
R7793:Eif4g1	UTSW	16	20688614	missense	probably benign	0.00
R8309:Eif4g1	UTSW	16	20688828	missense	probably benign	0.19
R8365:Eif4g1	UTSW	16	20683527	missense	probably damaging	1.00
R8726:Eif4g1	UTSW	16	20675482	missense	probably damaging	1.00
R8778:Eif4g1	UTSW	16	20673446	intron	probably benign
X0062:Eif4g1	UTSW	16	20684501	missense	probably damaging	1.00
X0065:Eif4g1	UTSW	16	20682726	missense	probably damaging	1.00
Z1176:Eif4g1	UTSW	16	20673408	intron	probably benign
Z1177:Eif4g1	UTSW	16	20683905	missense	probably benign	0.06
Z1177:Eif4g1	UTSW	16	20686366	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- AAACTGGGGAGCCGTATTGC -3'
(R):5'- ATCTGAAGGAGCCACAGGTG -3'

Sequencing Primer
(F):5'- TTGCCGAACCCATACTGGAAGTAG -3'
(R):5'- CACAGGTGGAGCAGGAGAG -3'

Posted On

2019-12-20