Mutagenetix > Incidental Mutation

Incidental Mutation 'R0196:Oas3'

60755

Institutional Source

Beutler Lab

Gene Symbol

Oas3

Ensembl Gene

ENSMUSG00000032661

Gene Name

2'-5' oligoadenylate synthetase 3

Synonyms

Oasl10, 2'-5' oligoadenylate synthetase-like 10

MMRRC Submission

038455-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R0196 (G1)

Quality Score

204

Status

Not validated

Chromosome

Chromosomal Location

120891163-120915726 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 120894210 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 39 (R39C)

Ref Sequence

ENSEMBL: ENSMUSP00000125873 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044833] [ENSMUST00000165820]

AlphaFold

Q8VI93

Predicted Effect

probably damaging
Transcript: ENSMUST00000044833
AA Change: R1047C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000035588
Gene: ENSMUSG00000032661
AA Change: R1047C

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	159	341	6.3e-83	PFAM
Pfam:OAS1_C	610	795	3.1e-78	PFAM
Pfam:NTP_transf_2	831	920	4.5e-11	PFAM
Pfam:OAS1_C	954	1136	9e-85	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000165820
AA Change: R39C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125873
Gene: ENSMUSG00000032661
AA Change: R39C

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	1	130	3.8e-53	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200795

Meta Mutation Damage Score

0.8791

Coding Region Coverage

1x: 98.8%
3x: 97.7%
10x: 93.8%
20x: 81.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933409G03Rik	A	G	2: 68,446,591 (GRCm39)		probably benign	Het
Aass	G	A	6: 23,109,519 (GRCm39)	P317L	probably damaging	Het
Abca12	T	A	1: 71,298,972 (GRCm39)	N2313I	possibly damaging	Het
Adamts12	T	C	15: 11,071,594 (GRCm39)	I46T	probably benign	Het
Adipoq	T	G	16: 22,965,393 (GRCm39)		probably null	Het
Amy1	A	T	3: 113,363,070 (GRCm39)	D92E	probably benign	Het
Asb15	G	A	6: 24,564,392 (GRCm39)	R282Q	probably damaging	Het
Bag6	G	C	17: 35,363,239 (GRCm39)	G693A	probably damaging	Het
Birc6	T	C	17: 74,887,282 (GRCm39)	I870T	possibly damaging	Het
Cand2	A	G	6: 115,766,463 (GRCm39)	K356R	probably damaging	Het
Cbfa2t3	T	C	8: 123,360,076 (GRCm39)	Q525R	possibly damaging	Het
Cd4	T	C	6: 124,844,769 (GRCm39)	R339G	probably damaging	Het
Cdh8	A	G	8: 99,917,066 (GRCm39)	S350P	probably damaging	Het
Cep295	A	T	9: 15,249,509 (GRCm39)	S469T	probably damaging	Het
Ckap2l	A	T	2: 129,127,342 (GRCm39)	S279T	probably benign	Het
Clnk	T	A	5: 38,927,282 (GRCm39)	N66Y	probably damaging	Het
Col27a1	A	T	4: 63,142,503 (GRCm39)	T64S	probably benign	Het
Crtc1	T	C	8: 70,838,871 (GRCm39)	D599G	probably damaging	Het
Cyp2c23	A	C	19: 44,000,795 (GRCm39)	I363S	probably damaging	Het
Dnah10	A	T	5: 124,911,139 (GRCm39)	I4519F	possibly damaging	Het
Dner	T	A	1: 84,348,553 (GRCm39)	I716F	probably damaging	Het
Dsel	T	G	1: 111,789,333 (GRCm39)	T401P	possibly damaging	Het
Egfr	A	G	11: 16,861,746 (GRCm39)	D1175G	probably benign	Het
Ephb3	A	T	16: 21,036,804 (GRCm39)	N343I	probably damaging	Het
Fbxw10	T	A	11: 62,768,070 (GRCm39)	F974I	probably benign	Het
Gfi1b	T	C	2: 28,503,786 (GRCm39)	Y138C	probably damaging	Het
Gm11168	T	A	9: 3,005,175 (GRCm39)	L6H	probably benign	Het
Grb10	A	C	11: 11,895,583 (GRCm39)	V247G	probably damaging	Het
Gstp2	A	T	19: 4,090,514 (GRCm39)		probably null	Het
Hars2	C	T	18: 36,922,257 (GRCm39)	Q291*	probably null	Het
Hyal4	G	T	6: 24,756,220 (GRCm39)	W146L	probably damaging	Het
Il22ra1	C	T	4: 135,461,556 (GRCm39)	T107I	possibly damaging	Het
Itga8	A	G	2: 12,209,540 (GRCm39)		probably null	Het
Klhl25	T	C	7: 75,515,450 (GRCm39)	S119P	probably damaging	Het
Krt81	C	A	15: 101,361,508 (GRCm39)	R24L	possibly damaging	Het
Lrrc8c	T	C	5: 105,754,636 (GRCm39)	V137A	probably benign	Het
Macrod2	A	T	2: 142,018,545 (GRCm39)	E226V	probably damaging	Het
Mcemp1	A	T	8: 3,718,201 (GRCm39)	Q165L	probably benign	Het
Mcpt9	T	A	14: 56,265,453 (GRCm39)	K82M	probably benign	Het
Mpzl3	A	G	9: 44,973,458 (GRCm39)	T66A	probably damaging	Het
Msh6	G	A	17: 88,287,788 (GRCm39)	V143I	possibly damaging	Het
Mug1	G	A	6: 121,815,684 (GRCm39)		probably null	Het
Ncr1	G	T	7: 4,343,972 (GRCm39)	C153F	probably damaging	Het
Nf1	T	A	11: 79,359,595 (GRCm39)	M1411K	possibly damaging	Het
Nf1	T	A	11: 79,469,098 (GRCm39)	V786D	probably damaging	Het
Nisch	T	C	14: 30,925,351 (GRCm39)		probably benign	Het
Nwd2	T	A	5: 63,963,694 (GRCm39)	Y1093N	probably benign	Het
Or1e32	A	T	11: 73,705,731 (GRCm39)	M59K	probably damaging	Het
Or7a36	C	T	10: 78,820,023 (GRCm39)	T133I	possibly damaging	Het
Oxa1l	T	C	14: 54,600,944 (GRCm39)	I139T	probably damaging	Het
P3h3	T	A	6: 124,822,235 (GRCm39)	N583Y	probably damaging	Het
Pcdh18	A	T	3: 49,711,147 (GRCm39)		probably null	Het
Pcnp	C	T	16: 55,844,896 (GRCm39)		probably benign	Het
Pdzd8	G	T	19: 59,289,563 (GRCm39)	D612E	probably benign	Het
Pi4kb	T	C	3: 94,906,261 (GRCm39)	S8P	probably damaging	Het
Pikfyve	T	G	1: 65,295,231 (GRCm39)	V1454G	possibly damaging	Het
Podn	T	C	4: 107,878,695 (GRCm39)	N246D	probably damaging	Het
Prg4	T	C	1: 150,330,243 (GRCm39)		probably benign	Het
R3hdm2	T	C	10: 127,320,390 (GRCm39)	Y523H	probably damaging	Het
Rpf1	T	A	3: 146,213,904 (GRCm39)	E231V	possibly damaging	Het
Slc16a10	C	T	10: 39,932,611 (GRCm39)	E317K	probably benign	Het
Slc34a1	A	T	13: 55,560,078 (GRCm39)	I435F	probably damaging	Het
Snx19	A	G	9: 30,344,683 (GRCm39)	D629G	probably damaging	Het
Tomm70a	T	C	16: 56,966,463 (GRCm39)	I472T	probably benign	Het
Trp53	A	G	11: 69,479,506 (GRCm39)	Y202C	probably damaging	Het
Ttc14	T	A	3: 33,863,403 (GRCm39)		probably benign	Het
Ugt1a1	C	T	1: 88,140,277 (GRCm39)	A185V	possibly damaging	Het
Usp28	A	G	9: 48,939,578 (GRCm39)	D655G	probably damaging	Het
Vmn1r215	C	T	13: 23,260,254 (GRCm39)	T98I	probably damaging	Het
Vmn2r121	G	T	X: 123,041,879 (GRCm39)	T426N	probably benign	Het
Vmn2r99	A	G	17: 19,614,835 (GRCm39)	N852D	probably benign	Het
Xrn2	T	A	2: 146,889,580 (GRCm39)	D654E	probably damaging	Het
Yju2	C	A	17: 56,271,653 (GRCm39)	D191E	probably damaging	Het
Zfp335	C	G	2: 164,738,065 (GRCm39)	A849P	possibly damaging	Het
Zfp954	C	T	7: 7,118,390 (GRCm39)	V385M	probably damaging	Het
Zmynd15	A	G	11: 70,355,052 (GRCm39)	T350A	probably damaging	Het

Other mutations in Oas3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00547:Oas3	APN	5	120,915,507 (GRCm39)	splice site	probably benign
IGL01095:Oas3	APN	5	120,910,954 (GRCm39)	missense	probably damaging	1.00
IGL01835:Oas3	APN	5	120,904,193 (GRCm39)	nonsense	probably null
IGL02006:Oas3	APN	5	120,907,300 (GRCm39)	missense	probably benign	0.00
IGL02811:Oas3	APN	5	120,902,387 (GRCm39)	missense	unknown
IGL03194:Oas3	APN	5	120,897,018 (GRCm39)	missense	probably damaging	1.00
R0066:Oas3	UTSW	5	120,896,940 (GRCm39)	missense	probably damaging	1.00
R0195:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0197:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0445:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0523:Oas3	UTSW	5	120,904,209 (GRCm39)	missense	unknown
R0592:Oas3	UTSW	5	120,909,214 (GRCm39)	missense	probably damaging	1.00
R0946:Oas3	UTSW	5	120,907,128 (GRCm39)	missense	unknown
R1354:Oas3	UTSW	5	120,908,065 (GRCm39)	missense	possibly damaging	0.94
R1642:Oas3	UTSW	5	120,915,639 (GRCm39)	missense	possibly damaging	0.90
R1681:Oas3	UTSW	5	120,907,973 (GRCm39)	missense	probably benign	0.22
R1844:Oas3	UTSW	5	120,898,045 (GRCm39)	missense	probably damaging	0.99
R1981:Oas3	UTSW	5	120,899,900 (GRCm39)	splice site	probably benign
R2443:Oas3	UTSW	5	120,915,553 (GRCm39)	missense	probably benign	0.35
R2902:Oas3	UTSW	5	120,896,982 (GRCm39)	missense	probably damaging	1.00
R3034:Oas3	UTSW	5	120,909,121 (GRCm39)	missense	probably damaging	1.00
R4565:Oas3	UTSW	5	120,909,104 (GRCm39)	missense	probably damaging	1.00
R4692:Oas3	UTSW	5	120,907,420 (GRCm39)	missense	probably benign	0.03
R4723:Oas3	UTSW	5	120,904,321 (GRCm39)	missense	unknown
R4812:Oas3	UTSW	5	120,899,212 (GRCm39)	unclassified	probably benign
R5288:Oas3	UTSW	5	120,895,055 (GRCm39)	missense	probably damaging	1.00
R5343:Oas3	UTSW	5	120,894,303 (GRCm39)	missense	possibly damaging	0.70
R5494:Oas3	UTSW	5	120,899,709 (GRCm39)	missense	unknown
R5688:Oas3	UTSW	5	120,896,867 (GRCm39)	missense	probably benign	0.31
R5894:Oas3	UTSW	5	120,895,019 (GRCm39)	missense	probably damaging	1.00
R5921:Oas3	UTSW	5	120,908,046 (GRCm39)	missense	probably damaging	1.00
R6037:Oas3	UTSW	5	120,907,384 (GRCm39)	missense	probably benign	0.41
R6037:Oas3	UTSW	5	120,907,384 (GRCm39)	missense	probably benign	0.41
R6066:Oas3	UTSW	5	120,910,989 (GRCm39)	missense	probably damaging	0.97
R6104:Oas3	UTSW	5	120,899,758 (GRCm39)	missense	unknown
R6134:Oas3	UTSW	5	120,907,113 (GRCm39)	missense	unknown
R6255:Oas3	UTSW	5	120,909,295 (GRCm39)	missense	probably benign	0.04
R6257:Oas3	UTSW	5	120,899,200 (GRCm39)	unclassified	probably benign
R6776:Oas3	UTSW	5	120,896,939 (GRCm39)	missense	probably damaging	1.00
R8022:Oas3	UTSW	5	120,895,031 (GRCm39)	missense	possibly damaging	0.91
R8137:Oas3	UTSW	5	120,915,565 (GRCm39)	missense	probably benign	0.07
R8967:Oas3	UTSW	5	120,896,907 (GRCm39)	missense	probably damaging	0.99
R9124:Oas3	UTSW	5	120,912,170 (GRCm39)	missense	probably damaging	1.00
R9287:Oas3	UTSW	5	120,892,754 (GRCm39)	missense	probably damaging	1.00
R9661:Oas3	UTSW	5	120,904,230 (GRCm39)	missense	unknown
R9745:Oas3	UTSW	5	120,899,284 (GRCm39)	missense	unknown
RF006:Oas3	UTSW	5	120,912,165 (GRCm39)	missense	probably damaging	1.00
X0024:Oas3	UTSW	5	120,899,793 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACAGGTGACCCAGAAGCTGACTAC -3'
(R):5'- TGCCAATGTGAACCTACACATCTCC -3'

Sequencing Primer
(F):5'- AGCACCACTCTGGGAAGC -3'
(R):5'- GTGAACCTACACATCTCCTGTCC -3'

Posted On

2013-07-24