Incidental Mutation 'R7866:Top6bl'
ID 607813
Institutional Source Beutler Lab
Gene Symbol Top6bl
Ensembl Gene ENSMUSG00000071691
Gene Name TOP6B like initiator of meiotic double strand breaks
Synonyms Top6bl, Gm960
MMRRC Submission 045918-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.071) question?
Stock # R7866 (G1)
Quality Score 225.009
Status Not validated
Chromosome 19
Chromosomal Location 4675762-4748696 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 4748514 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Serine at position 61 (R61S)
Ref Sequence ENSEMBL: ENSMUSP00000136515 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000096325] [ENSMUST00000177696] [ENSMUST00000225896]
AlphaFold J3QMY9
Predicted Effect probably benign
Transcript: ENSMUST00000096325
SMART Domains Protein: ENSMUSP00000094049
Gene: ENSMUSG00000071691

DomainStartEndE-ValueType
Pfam:DUF4554 120 162 1.6e-17 PFAM
Predicted Effect unknown
Transcript: ENSMUST00000177696
AA Change: R61S
SMART Domains Protein: ENSMUSP00000136515
Gene: ENSMUSG00000071691
AA Change: R61S

DomainStartEndE-ValueType
low complexity region 65 79 N/A INTRINSIC
low complexity region 106 122 N/A INTRINSIC
low complexity region 128 140 N/A INTRINSIC
Pfam:DUF4554 274 719 5.3e-206 PFAM
low complexity region 720 731 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000225896
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired double-strand break formation that imapires female and male meiosis and results in no spermatids and reduced primary and primordial follicle numbers. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 59 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcg1 C T 17: 31,317,269 (GRCm39) R194* probably null Het
Actl6a A G 3: 32,766,262 (GRCm39) T39A possibly damaging Het
Adamts6 T A 13: 104,550,257 (GRCm39) C624* probably null Het
Adgrl1 T C 8: 84,664,564 (GRCm39) probably null Het
Ap3m2 A G 8: 23,289,674 (GRCm39) V143A probably benign Het
Ap5m1 T C 14: 49,311,218 (GRCm39) V96A probably damaging Het
Aqp5 T A 15: 99,489,424 (GRCm39) V91E probably damaging Het
Atp6v0b A T 4: 117,742,350 (GRCm39) I181N probably damaging Het
Ccp110 T A 7: 118,322,241 (GRCm39) M632K probably benign Het
Cd44 A T 2: 102,672,604 (GRCm39) probably null Het
Cidea G A 18: 67,491,854 (GRCm39) R38Q probably damaging Het
Cnn3 T C 3: 121,245,042 (GRCm39) I86T probably benign Het
Col14a1 C A 15: 55,252,016 (GRCm39) D557E unknown Het
Col6a6 T C 9: 105,566,760 (GRCm39) Y2245C probably damaging Het
Dhrs4 A T 14: 55,725,092 (GRCm39) N196Y probably damaging Het
Dnah8 T C 17: 31,093,901 (GRCm39) V4665A possibly damaging Het
Dpep2 T C 8: 106,716,113 (GRCm39) T267A Het
Dtx3l T A 16: 35,759,120 (GRCm39) Q43L probably benign Het
Ednrb A G 14: 104,080,738 (GRCm39) S59P probably benign Het
Eml4 A G 17: 83,758,126 (GRCm39) T435A probably benign Het
Fam135b A T 15: 71,333,925 (GRCm39) F1090I probably benign Het
Filip1 A G 9: 79,726,225 (GRCm39) V798A probably benign Het
Fnip1 T G 11: 54,356,228 (GRCm39) probably benign Het
Gabrb2 C T 11: 42,378,050 (GRCm39) Q89* probably null Het
Gtf2ird1 A T 5: 134,392,063 (GRCm39) V882E probably benign Het
Gucy1a2 T A 9: 3,532,804 (GRCm39) M1K probably null Het
Ift70b A G 2: 75,766,963 (GRCm39) S597P possibly damaging Het
Igkv10-96 T A 6: 68,609,025 (GRCm39) D90V possibly damaging Het
Igkv4-54 G A 6: 69,608,740 (GRCm39) R60C probably benign Het
Igkv7-33 G T 6: 70,035,847 (GRCm39) A45D probably damaging Het
Klk1b22 A T 7: 43,762,168 (GRCm39) I15F possibly damaging Het
Lamp3 T A 16: 19,518,490 (GRCm39) D249V probably benign Het
Lrrfip2 T C 9: 111,022,149 (GRCm39) V125A possibly damaging Het
Mprip G A 11: 59,643,756 (GRCm39) R638H possibly damaging Het
Muc5ac A G 7: 141,349,589 (GRCm39) M501V probably benign Het
Myo5a C T 9: 75,111,034 (GRCm39) P1509S probably damaging Het
Ngdn A G 14: 55,258,554 (GRCm39) Y63C probably damaging Het
Oit3 A G 10: 59,259,852 (GRCm39) V517A probably benign Het
Or8g21 A T 9: 38,906,027 (GRCm39) S235T not run Het
Pcdhb18 T A 18: 37,623,512 (GRCm39) F281I probably damaging Het
Pkd1 C A 17: 24,809,881 (GRCm39) Q3520K probably benign Het
Plxnb1 T A 9: 108,929,525 (GRCm39) I127N probably damaging Het
Ppfia2 A T 10: 106,655,390 (GRCm39) N319I probably damaging Het
Pxn T C 5: 115,686,665 (GRCm39) S386P possibly damaging Het
Ralgps2 C T 1: 156,714,738 (GRCm39) V104I probably benign Het
Rp1 C T 1: 4,417,924 (GRCm39) V1063I probably benign Het
Slc10a5 A T 3: 10,399,532 (GRCm39) F376Y probably damaging Het
Snd1 T A 6: 28,527,724 (GRCm39) I277N probably damaging Het
Spc25 A G 2: 69,036,406 (GRCm39) probably null Het
Tex29 A G 8: 11,894,055 (GRCm39) N6D unknown Het
Ttc6 G A 12: 57,721,435 (GRCm39) A975T probably damaging Het
Uba6 T C 5: 86,320,560 (GRCm39) E13G probably damaging Het
Ugcg T G 4: 59,211,927 (GRCm39) C98G possibly damaging Het
Ugt2b36 T A 5: 87,240,190 (GRCm39) D65V probably damaging Het
Unc93b1 A T 19: 3,985,243 (GRCm39) D17V not run Het
Usf1 G T 1: 171,245,462 (GRCm39) W291C unknown Het
Vps29 T C 5: 122,500,180 (GRCm39) W97R possibly damaging Het
Zbtb6 T C 2: 37,319,577 (GRCm39) E117G probably damaging Het
Zfp735 A C 11: 73,601,629 (GRCm39) D191A probably benign Het
Other mutations in Top6bl
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01303:Top6bl APN 19 4,709,510 (GRCm39) missense possibly damaging 0.93
IGL01678:Top6bl APN 19 4,722,193 (GRCm39) missense possibly damaging 0.94
IGL02160:Top6bl APN 19 4,713,612 (GRCm39) missense probably damaging 1.00
IGL02308:Top6bl APN 19 4,713,583 (GRCm39) missense probably damaging 1.00
IGL03375:Top6bl APN 19 4,748,206 (GRCm39) missense probably benign 0.26
R0485:Top6bl UTSW 19 4,708,442 (GRCm39) missense probably damaging 1.00
R0671:Top6bl UTSW 19 4,676,216 (GRCm39) missense probably damaging 0.97
R1583:Top6bl UTSW 19 4,702,199 (GRCm39) missense probably damaging 1.00
R2049:Top6bl UTSW 19 4,748,633 (GRCm39) utr 5 prime probably benign
R3956:Top6bl UTSW 19 4,742,525 (GRCm39) missense probably benign 0.13
R4554:Top6bl UTSW 19 4,699,847 (GRCm39) missense possibly damaging 0.95
R4635:Top6bl UTSW 19 4,748,524 (GRCm39) utr 5 prime probably benign
R4717:Top6bl UTSW 19 4,675,901 (GRCm39) unclassified probably benign
R4996:Top6bl UTSW 19 4,676,112 (GRCm39) missense probably benign 0.09
R5133:Top6bl UTSW 19 4,708,449 (GRCm39) missense probably damaging 1.00
R5752:Top6bl UTSW 19 4,676,048 (GRCm39) missense probably benign 0.28
R6277:Top6bl UTSW 19 4,677,250 (GRCm39) nonsense probably null
R6348:Top6bl UTSW 19 4,722,106 (GRCm39) missense probably damaging 0.96
R7386:Top6bl UTSW 19 4,713,586 (GRCm39) nonsense probably null
R7673:Top6bl UTSW 19 4,695,661 (GRCm39) missense probably damaging 1.00
R7874:Top6bl UTSW 19 4,708,451 (GRCm39) missense probably damaging 1.00
R8892:Top6bl UTSW 19 4,699,721 (GRCm39) missense possibly damaging 0.52
R8983:Top6bl UTSW 19 4,695,714 (GRCm39) missense possibly damaging 0.66
R9245:Top6bl UTSW 19 4,746,068 (GRCm39) missense possibly damaging 0.84
R9522:Top6bl UTSW 19 4,677,274 (GRCm39) missense probably benign 0.02
Z1176:Top6bl UTSW 19 4,675,931 (GRCm39) missense unknown
Predicted Primers PCR Primer
(F):5'- TTCTGTAAGGAGAGCGGCTG -3'
(R):5'- CCCTCTGTTAGATGTGGGAAG -3'

Sequencing Primer
(F):5'- ACTGCCAGTTGGTCACAG -3'
(R):5'- AAGTGGAGCCTGGTCCCAG -3'
Posted On 2019-12-20