Incidental Mutation 'R7869:Ddt'
Institutional Source Beutler Lab
Gene Symbol Ddt
Ensembl Gene ENSMUSG00000001666
Gene NameD-dopachrome tautomerase
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R7869 (G1)
Quality Score225.009
Status Not validated
Chromosomal Location75771230-75773414 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 75773226 bp
Amino Acid Change Lysine to Arginine at position 33 (K33R)
Ref Sequence ENSEMBL: ENSMUSP00000001716 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000001715] [ENSMUST00000001716] [ENSMUST00000172820] [ENSMUST00000173537] [ENSMUST00000174187]
PDB Structure
D-Dopachrome tautomerase (D-DT)/ macrophage migration inhibitory factor 2 (MIF2) complexed with inhibitor 4-IPP [X-RAY DIFFRACTION]
Predicted Effect probably benign
Transcript: ENSMUST00000001715
SMART Domains Protein: ENSMUSP00000001715
Gene: ENSMUSG00000001665

Pfam:GST_N 1 76 2.2e-13 PFAM
Pfam:GST_N_3 5 82 2.4e-13 PFAM
Pfam:GST_N_2 11 77 6.2e-10 PFAM
Pfam:GST_C 111 201 5e-9 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000001716
AA Change: K33R

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000001716
Gene: ENSMUSG00000001666
AA Change: K33R

Pfam:MIF 2 116 8.5e-55 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000133869
SMART Domains Protein: ENSMUSP00000134134
Gene: ENSMUSG00000001665

PDB:2C3T|D 2 100 3e-52 PDB
SCOP:d1ljra1 6 107 4e-14 SMART
low complexity region 118 134 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000172820
SMART Domains Protein: ENSMUSP00000134561
Gene: ENSMUSG00000001666

Pfam:MIF 1 71 3.3e-32 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000173512
SMART Domains Protein: ENSMUSP00000134112
Gene: ENSMUSG00000092360

Pfam:GST_N 2 75 3.2e-13 PFAM
Pfam:GST_N_3 4 81 2.6e-13 PFAM
Pfam:GST_N_2 10 76 6.7e-10 PFAM
Pfam:MIF 159 252 8.5e-35 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000173537
SMART Domains Protein: ENSMUSP00000133498
Gene: ENSMUSG00000001665

Pfam:GST_N 3 76 5e-12 PFAM
Pfam:GST_N_3 5 82 1.2e-13 PFAM
Pfam:GST_N_2 11 77 6.1e-12 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000174187
SMART Domains Protein: ENSMUSP00000133576
Gene: ENSMUSG00000001665

PDB:2C3T|D 1 38 7e-16 PDB
SCOP:d1ljra2 1 38 6e-5 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 76 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2900026A02Rik G A 5: 113,184,135 R738C possibly damaging Het
Acot12 G A 13: 91,771,725 V260M probably benign Het
Alkbh8 A T 9: 3,359,503 H264L probably damaging Het
Arfgef2 C T 2: 166,873,703 T1356I probably damaging Het
Arhgap27 A T 11: 103,360,304 N199K probably damaging Het
Asic2 T C 11: 81,967,998 R63G probably damaging Het
Aspa T A 11: 73,313,552 M197L probably benign Het
BC005537 C T 13: 24,803,399 R7W possibly damaging Het
Brdt A T 5: 107,370,179 I723L probably benign Het
C2cd3 T A 7: 100,469,491 F2312Y probably damaging Het
Cd19 T C 7: 126,410,526 N456S probably damaging Het
Ceacam1 G T 7: 25,476,529 A80E probably damaging Het
Cep135 T C 5: 76,640,956 V1132A probably benign Het
Cep85 A G 4: 134,132,298 C687R probably damaging Het
Cln5 A G 14: 103,076,065 Y251C probably damaging Het
Crygc A G 1: 65,071,855 M91T possibly damaging Het
Dhfr G A 13: 92,357,963 W58* probably null Het
Dhx40 T C 11: 86,797,706 E297G probably benign Het
Dner T C 1: 84,383,881 Y669C probably benign Het
Dpys G T 15: 39,793,386 F428L probably damaging Het
Dspp A C 5: 104,175,665 T225P probably damaging Het
Elac2 A G 11: 64,999,387 K656E probably damaging Het
Ern1 T A 11: 106,459,019 R24* probably null Het
F2rl2 T C 13: 95,701,011 I188T probably damaging Het
Fam83b C T 9: 76,492,144 C559Y possibly damaging Het
Fat1 T G 8: 45,051,222 F4584C probably benign Het
Gabra6 G A 11: 42,316,495 T254I possibly damaging Het
Garem1 T C 18: 21,299,700 D24G probably damaging Het
Gas2l2 A T 11: 83,421,990 L832* probably null Het
Gpr68 C T 12: 100,879,238 D16N probably benign Het
Il17b T C 18: 61,692,409 I172T probably damaging Het
Ildr2 T G 1: 166,309,292 S567A probably benign Het
Kalrn A G 16: 33,988,847 V2557A probably damaging Het
Kif1b A G 4: 149,184,376 S1627P probably benign Het
Kif5a G A 10: 127,243,474 T285M probably damaging Het
Lrif1 G A 3: 106,733,143 probably null Het
Lrrn2 C T 1: 132,939,378 T727I unknown Het
Lsg1 T C 16: 30,564,722 E560G probably benign Het
Map4k4 C A 1: 39,974,044 Q100K unknown Het
Mipep A T 14: 60,802,936 R302W probably damaging Het
Morf4l1 G T 9: 90,093,791 T316K probably damaging Het
Mov10 A T 3: 104,804,678 V126E probably damaging Het
Muc2 T A 7: 141,749,734 C559S Het
Myh7 A G 14: 54,989,073 Y410H probably damaging Het
Nf1 T A 11: 79,418,588 Y433N probably damaging Het
Nmd3 T C 3: 69,726,417 L79P probably damaging Het
Nol10 G T 12: 17,358,238 S155I probably null Het
Olfr1208 T C 2: 88,897,064 I178V probably benign Het
Olfr134 T C 17: 38,175,939 L285P possibly damaging Het
Olfr1448 A G 19: 12,919,547 I254T probably benign Het
Olfr668 T A 7: 104,925,104 Y220F probably damaging Het
Pde3b T C 7: 114,494,687 S389P probably benign Het
Polr1c C T 17: 46,244,891 G188D probably benign Het
Prpf31 A G 7: 3,630,860 M65V probably benign Het
Pum2 A G 12: 8,713,595 T251A probably benign Het
Rabgap1 G A 2: 37,487,130 V325I probably benign Het
Repin1 G T 6: 48,597,345 E403* probably null Het
Rpl15 G T 14: 18,269,017 Y203* probably null Het
Sac3d1 G A 19: 6,118,426 P54L possibly damaging Het
Serpina3g A G 12: 104,240,251 I104V probably benign Het
Setd2 C T 9: 110,550,014 Q966* probably null Het
Simc1 G T 13: 54,503,900 D9Y unknown Het
Slc5a8 T C 10: 88,921,705 L519S probably benign Het
Srcap C A 7: 127,539,194 Q1179K possibly damaging Het
Srd5a3 T C 5: 76,147,736 L91P probably damaging Het
Stab1 T C 14: 31,154,472 T887A probably benign Het
Sult2a6 A G 7: 14,254,812 F8L not run Het
Thsd7a A T 6: 12,471,124 C498* probably null Het
Timp4 T C 6: 115,250,394 I75V probably benign Het
Tspyl4 T A 10: 34,298,100 M196K probably damaging Het
Ubr2 T C 17: 46,991,008 E172G probably benign Het
Ubr5 G A 15: 37,979,832 A2434V probably null Het
Unc13c T G 9: 73,694,877 L1362F probably damaging Het
Vmn2r11 A G 5: 109,052,120 V489A probably damaging Het
Vps39 T C 2: 120,339,394 T226A possibly damaging Het
Zan C T 5: 137,473,601 E185K probably damaging Het
Other mutations in Ddt
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0206:Ddt UTSW 10 75772885 start codon destroyed probably null 0.17
R0854:Ddt UTSW 10 75771495 missense probably benign 0.02
R1853:Ddt UTSW 10 75773304 missense possibly damaging 0.79
R3107:Ddt UTSW 10 75772763 missense probably benign 0.00
R7552:Ddt UTSW 10 75773214 critical splice donor site probably null
R7952:Ddt UTSW 10 75773226 missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On2019-12-20