|Institutional Source||Beutler Lab|
|Gene Name||tripartite motif-containing 28|
|Synonyms||KRIP-1, Tif1b, KAP-1, MommeD9|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R7878 (G1)|
|Chromosomal Location||12999114-13031035 bp(+) (GRCm38)|
|Type of Mutation||start gained|
|DNA Base Change (assembly)||G to T at 13024362 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000005705 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000005705]|
|Predicted Effect||probably benign
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted disruption of this gene develop normally until the blastocyst stage and undergo uterine implantation, but become arrested at the early egg-cylinder stage, fail to gastrulate, and are completely resorbed by E8.5. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Trim28||
(F):5'- GCCTAAGGTTCGGCTCTG -3'
(R):5'- TGTCGAGATGCGGCCAAG -3'
(F):5'- TAAGGTTCGGCTCTGCAGGAC -3'
(R):5'- CTCGCTTCTGAGACACT -3'