Mutagenetix > Incidental Mutation

Incidental Mutation 'R7879:Or4x6'

608616

Institutional Source

Beutler Lab

Gene Symbol

Or4x6

Ensembl Gene

ENSMUSG00000084336

Gene Name

olfactory receptor family 4 subfamily X member 6

Synonyms

GA_x6K02T2Q125-51551618-51550689, Olfr1269, MOR228-3

MMRRC Submission

045931-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R7879 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

89949011-89949940 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 89949185 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 252 (C252*)

Ref Sequence

ENSEMBL: ENSMUSP00000150470 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099756] [ENSMUST00000117787] [ENSMUST00000214404] [ENSMUST00000216493]

AlphaFold

Q8VEZ2

Predicted Effect

probably null
Transcript: ENSMUST00000099756
AA Change: C252*

SMART Domains

Protein: ENSMUSP00000097345
Gene: ENSMUSG00000084336
AA Change: C252*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	303	3.8e-50	PFAM
Pfam:7TM_GPCR_Srsx	33	300	1.2e-5	PFAM
Pfam:7tm_1	39	285	5.9e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117787

Predicted Effect

probably null
Transcript: ENSMUST00000214404
AA Change: C252*

Predicted Effect

probably null
Transcript: ENSMUST00000216493
AA Change: C252*

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930449I24Rik	T	C	5: 146,439,662 (GRCm39)	I81T	probably benign	Het
Acnat2	G	A	4: 49,383,299 (GRCm39)	P85S	probably damaging	Het
Akr1c19	G	A	13: 4,286,223 (GRCm39)	V74M	probably damaging	Het
Alb	C	A	5: 90,620,507 (GRCm39)	T539K	probably benign	Het
B4galnt2	G	A	11: 95,760,223 (GRCm39)	T268I	possibly damaging	Het
Bcl7b	G	A	5: 135,205,986 (GRCm39)	E58K	possibly damaging	Het
Calr3	C	T	8: 73,178,487 (GRCm39)	S372N	unknown	Het
Cdcp3	T	A	7: 130,844,871 (GRCm39)	V588E	probably damaging	Het
Cdh20	A	T	1: 104,875,047 (GRCm39)		probably null	Het
Cdh20	A	C	1: 109,976,677 (GRCm39)	E114A	probably benign	Het
Cep70	A	G	9: 99,144,686 (GRCm39)	R74G	possibly damaging	Het
Chrna6	A	G	8: 27,897,109 (GRCm39)	F256S	probably damaging	Het
Cic	A	G	7: 24,984,551 (GRCm39)	T996A	probably benign	Het
Clstn2	A	G	9: 97,351,817 (GRCm39)	V536A	possibly damaging	Het
Csmd1	A	T	8: 16,441,820 (GRCm39)	M348K	probably benign	Het
Dnah11	A	C	12: 118,004,744 (GRCm39)	D2192E	probably damaging	Het
Dnhd1	A	G	7: 105,352,646 (GRCm39)	I2600V	probably benign	Het
Dock3	A	C	9: 106,785,700 (GRCm39)	S185A	probably benign	Het
Dock4	A	T	12: 40,780,083 (GRCm39)	D628V	possibly damaging	Het
Dzank1	A	T	2: 144,333,718 (GRCm39)	S371T	probably benign	Het
Fam83b	A	T	9: 76,399,737 (GRCm39)	N455K	possibly damaging	Het
Fcrlb	T	C	1: 170,736,365 (GRCm39)	Y137C	probably damaging	Het
Gtf2i	G	A	5: 134,295,471 (GRCm39)	T327M	possibly damaging	Het
Hspa5	T	A	2: 34,665,941 (GRCm39)	M595K	probably benign	Het
Htt	C	T	5: 34,981,252 (GRCm39)	A875V	probably benign	Het
Hvcn1	G	A	5: 122,376,701 (GRCm39)		probably null	Het
Igsf10	A	G	3: 59,238,145 (GRCm39)	S679P	probably damaging	Het
Itsn2	A	G	12: 4,751,265 (GRCm39)	H1285R	probably benign	Het
Kcnq4	T	C	4: 120,559,632 (GRCm39)	T523A	probably benign	Het
Kif6	T	A	17: 50,139,214 (GRCm39)	I562N	probably benign	Het
Klhl9	C	A	4: 88,638,575 (GRCm39)	W555C	probably damaging	Het
Krt78	T	C	15: 101,856,624 (GRCm39)	T423A	probably benign	Het
Mapk10	C	T	5: 103,111,362 (GRCm39)	V391I	probably benign	Het
Noa1	T	C	5: 77,445,044 (GRCm39)	M592V	probably benign	Het
Npy5r	A	G	8: 67,133,968 (GRCm39)	M275T	possibly damaging	Het
Nuak2	T	A	1: 132,259,695 (GRCm39)	V499E	probably benign	Het
Or6c213	T	C	10: 129,574,559 (GRCm39)	I76V	probably benign	Het
Otogl	T	C	10: 107,612,970 (GRCm39)	E2052G	probably benign	Het
Pard3b	T	A	1: 62,198,670 (GRCm39)	C253S	possibly damaging	Het
Pclo	T	C	5: 14,727,228 (GRCm39)	S2029P	unknown	Het
Plekhg3	A	G	12: 76,612,343 (GRCm39)	I401M	probably damaging	Het
Polg	A	T	7: 79,100,392 (GRCm39)	C1140S	probably benign	Het
Prl7c1	A	T	13: 27,962,817 (GRCm39)	L62H	probably damaging	Het
Psmf1	T	A	2: 151,576,163 (GRCm39)	E115V	probably benign	Het
Ptpn9	A	T	9: 56,964,010 (GRCm39)	N381I	possibly damaging	Het
Pzp	A	G	6: 128,465,979 (GRCm39)	S1234P	probably benign	Het
Qrich1	A	G	9: 108,436,485 (GRCm39)	T728A	possibly damaging	Het
Ralgps2	C	A	1: 156,656,636 (GRCm39)	A323S	probably benign	Het
Rimbp3	T	C	16: 17,028,910 (GRCm39)	V778A	possibly damaging	Het
Scn1a	A	T	2: 66,116,349 (GRCm39)	L430*	probably null	Het
Sema7a	A	T	9: 57,862,363 (GRCm39)	I189F	probably damaging	Het
Slc22a8	T	C	19: 8,571,386 (GRCm39)	I39T	probably benign	Het
Stap2	T	C	17: 56,309,023 (GRCm39)	T115A	probably benign	Het
Stmn4	A	G	14: 66,595,388 (GRCm39)	I138V	probably benign	Het
Suds3	C	T	5: 117,236,335 (GRCm39)		probably null	Het
Taf4	G	A	2: 179,573,822 (GRCm39)	T682M	probably damaging	Het
Tet1	A	C	10: 62,714,825 (GRCm39)	D323E	probably benign	Het
Tia1	A	G	6: 86,401,347 (GRCm39)	D140G	probably damaging	Het
Trio	C	T	15: 27,852,010 (GRCm39)	R827H	possibly damaging	Het
Ttn	A	G	2: 76,625,397 (GRCm39)		probably null	Het
Unc80	T	C	1: 66,549,866 (GRCm39)	V708A	probably benign	Het
Vmn1r194	A	T	13: 22,428,772 (GRCm39)	T130S	probably benign	Het
Wfikkn2	A	G	11: 94,129,755 (GRCm39)	C129R	probably damaging	Het
Zkscan5	A	C	5: 145,157,676 (GRCm39)	H726P	probably damaging	Het

Other mutations in Or4x6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01389:Or4x6	APN	2	89,949,412 (GRCm39)	missense	probably damaging	1.00
IGL01518:Or4x6	APN	2	89,949,315 (GRCm39)	missense	possibly damaging	0.95
IGL01889:Or4x6	APN	2	89,949,309 (GRCm39)	missense	possibly damaging	0.84
IGL02629:Or4x6	APN	2	89,949,201 (GRCm39)	nonsense	probably null
R0732:Or4x6	UTSW	2	89,949,666 (GRCm39)	missense	probably benign	0.20
R1446:Or4x6	UTSW	2	89,949,202 (GRCm39)	missense	probably damaging	0.98
R1938:Or4x6	UTSW	2	89,949,427 (GRCm39)	missense	probably damaging	0.97
R4526:Or4x6	UTSW	2	89,949,016 (GRCm39)	missense	probably benign	0.01
R4786:Or4x6	UTSW	2	89,949,351 (GRCm39)	missense	possibly damaging	0.95
R4792:Or4x6	UTSW	2	89,949,174 (GRCm39)	missense	possibly damaging	0.95
R4925:Or4x6	UTSW	2	89,949,121 (GRCm39)	missense	probably damaging	1.00
R5152:Or4x6	UTSW	2	89,949,465 (GRCm39)	missense	probably damaging	0.98
R5296:Or4x6	UTSW	2	89,949,043 (GRCm39)	missense	probably damaging	0.97
R5450:Or4x6	UTSW	2	89,949,013 (GRCm39)	makesense	probably null
R5536:Or4x6	UTSW	2	89,949,183 (GRCm39)	missense	probably benign	0.00
R7572:Or4x6	UTSW	2	89,949,087 (GRCm39)	missense	probably damaging	1.00
R7695:Or4x6	UTSW	2	89,949,207 (GRCm39)	missense	probably benign	0.08
R7938:Or4x6	UTSW	2	89,949,173 (GRCm39)	nonsense	probably null
R8280:Or4x6	UTSW	2	89,949,742 (GRCm39)	missense	probably benign	0.27

Predicted Primers

PCR Primer
(F):5'- GTTTGGACTCAGCAGCATTCC -3'
(R):5'- GCTCAGACACCTTCCTCATTGG -3'

Sequencing Primer
(F):5'- GGACTCAGCAGCATTCCAATCC -3'
(R):5'- AGACACCTTCCTCATTGGTTTGC -3'

Posted On

2019-12-20