|Institutional Source||Beutler Lab|
|Gene Name||prostaglandin E synthase 2|
|Synonyms||GBF1, GBF-1, 0610038H10Rik|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R7881 (G1)|
|Chromosomal Location||32395896-32405772 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 32402231 bp|
|Amino Acid Change||Methionine to Lysine at position 353 (M353K)|
|Ref Sequence||ENSEMBL: ENSMUSP00000028162 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028162] [ENSMUST00000123714]|
|Predicted Effect||probably damaging
AA Change: M353K
PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
AA Change: M353K
|Predicted Effect||probably benign
|Meta Mutation Damage Score||0.6612|
|Coding Region Coverage||
|Validation Efficiency||97% (63/65)|
FUNCTION: The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate, which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However, a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein, but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE), possibly via an interaction with CAAAT/enhancer-binding protein-beta. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal basal prostaglandin E2 (PGE2) protein levels in the lactating mammary gland. Mice homozygous for a different knock-out allele exhibit increased sensitivity to IgE antigen-dependent passive cutaneous anaphylaxis. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ptges2||
(F):5'- TAGACCCTATCCCTGAGCAGTC -3'
(R):5'- TGGACCAGAGACAAAATGACTC -3'
(F):5'- ATCCCTGAGCAGTCTTGTAAG -3'
(R):5'- AAAATGACTCTGCCCTCTGTGAG -3'