|Institutional Source||Beutler Lab|
|Gene Name||phosphoinositide-3-kinase regulatory subunit 5|
|Is this an essential gene?||Probably non essential (E-score: 0.103)|
|Stock #||R7885 (G1)|
|Chromosomal Location||68432121-68497849 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 68492702 bp|
|Amino Acid Change||Alanine to Valine at position 449 (A449V)|
|Ref Sequence||ENSEMBL: ENSMUSP00000021283 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000021283]|
|Predicted Effect||possibly damaging
AA Change: A449V
PolyPhen 2 Score 0.573 (Sensitivity: 0.88; Specificity: 0.91)
AA Change: A449V
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit significantly reduced neutrophil chemotaxis and chemokinesis in vitro and impaired neutrophil recruitment into the peritoneum in a model of thioglycollate-induced aseptic peritonitis. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Pik3r5||
(F):5'- AGGAAAACTCAGAGTGGCCC -3'
(R):5'- TTCCCTGAAATCCGATCAGAG -3'
(F):5'- TCAGAGTGGCCCCAAAAGC -3'
(R):5'- CAACCCGTAGTGTGGAAGCTTTG -3'