Mutagenetix > Incidental Mutation

Incidental Mutation 'R7885:Zfp708'

609027

Institutional Source

Beutler Lab

Gene Symbol

Zfp708

Ensembl Gene

ENSMUSG00000058883

Gene Name

zinc finger protein 708

Synonyms

MMRRC Submission

045937-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R7885 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

67217463-67246040 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 67222193 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 62 (D62E)

Ref Sequence

ENSEMBL: ENSMUSP00000105364 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109742] [ENSMUST00000109743] [ENSMUST00000186303] [ENSMUST00000190566]

AlphaFold

F8VPP0

Predicted Effect

probably benign
Transcript: ENSMUST00000109742
AA Change: D62E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000105364
Gene: ENSMUSG00000058883
AA Change: D62E

Domain	Start	End	E-Value	Type
KRAB	5	65	3.75e-28	SMART
ZnF_C2H2	81	103	1.12e-3	SMART
ZnF_C2H2	109	131	5.14e-3	SMART
ZnF_C2H2	137	159	1.1e-2	SMART
ZnF_C2H2	165	187	6.78e-3	SMART
ZnF_C2H2	193	215	1.47e-3	SMART
ZnF_C2H2	221	243	7.78e-3	SMART
ZnF_C2H2	249	271	1.95e-3	SMART
ZnF_C2H2	277	299	5.21e-4	SMART
ZnF_C2H2	305	327	7.9e-4	SMART
ZnF_C2H2	333	355	8.34e-3	SMART
ZnF_C2H2	361	383	1.3e-4	SMART
ZnF_C2H2	389	411	4.87e-4	SMART
ZnF_C2H2	417	439	5.14e-3	SMART
ZnF_C2H2	445	467	1.2e-3	SMART
ZnF_C2H2	473	495	1.72e-4	SMART
ZnF_C2H2	501	523	1.6e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109743
AA Change: D74E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000105365
Gene: ENSMUSG00000058883
AA Change: D74E

Domain	Start	End	E-Value	Type
KRAB	17	77	3.75e-28	SMART
ZnF_C2H2	93	115	1.12e-3	SMART
ZnF_C2H2	121	143	5.14e-3	SMART
ZnF_C2H2	149	171	1.1e-2	SMART
ZnF_C2H2	177	199	6.78e-3	SMART
ZnF_C2H2	205	227	1.47e-3	SMART
ZnF_C2H2	233	255	7.78e-3	SMART
ZnF_C2H2	261	283	1.95e-3	SMART
ZnF_C2H2	289	311	5.21e-4	SMART
ZnF_C2H2	317	339	7.9e-4	SMART
ZnF_C2H2	345	367	8.34e-3	SMART
ZnF_C2H2	373	395	1.3e-4	SMART
ZnF_C2H2	401	423	4.87e-4	SMART
ZnF_C2H2	429	451	5.14e-3	SMART
ZnF_C2H2	457	479	1.2e-3	SMART
ZnF_C2H2	485	507	1.72e-4	SMART
ZnF_C2H2	513	535	1.6e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000186303

SMART Domains

Protein: ENSMUSP00000140296
Gene: ENSMUSG00000100235

Domain	Start	End	E-Value	Type
KRAB	1	32	9.7e-3	SMART
ZnF_C2H2	47	69	1.8e-6	SMART
ZnF_C2H2	75	97	6.7e-5	SMART
ZnF_C2H2	103	125	7.6e-6	SMART
ZnF_C2H2	131	153	7.6e-6	SMART
ZnF_C2H2	159	181	1.4e-7	SMART
ZnF_C2H2	187	209	7.6e-6	SMART
ZnF_C2H2	215	237	5.5e-5	SMART
ZnF_C2H2	243	265	8.9e-7	SMART
ZnF_C2H2	271	293	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000190566
AA Change: D29E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000139928
Gene: ENSMUSG00000058883
AA Change: D29E

Domain	Start	End	E-Value	Type
KRAB	1	32	1.6e-3	SMART
ZnF_C2H2	48	70	4.7e-6	SMART
ZnF_C2H2	76	98	2.1e-5	SMART
ZnF_C2H2	104	126	4.6e-5	SMART
ZnF_C2H2	132	154	2.9e-5	SMART
ZnF_C2H2	160	182	6.3e-6	SMART
ZnF_C2H2	188	210	3.3e-5	SMART
ZnF_C2H2	216	238	8.2e-6	SMART
ZnF_C2H2	244	266	2.2e-6	SMART
ZnF_C2H2	272	294	3.4e-6	SMART
ZnF_C2H2	300	322	3.6e-5	SMART
ZnF_C2H2	328	350	5.5e-7	SMART
ZnF_C2H2	356	378	2.1e-6	SMART
ZnF_C2H2	384	406	2.1e-5	SMART
ZnF_C2H2	412	434	5e-6	SMART
ZnF_C2H2	440	462	7.2e-7	SMART
ZnF_C2H2	468	490	6.8e-7	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

All alleles(1) : Targeted(1)

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adarb1	A	G	10: 77,131,542 (GRCm39)	V649A	possibly damaging	Het
Arglu1	C	T	8: 8,717,337 (GRCm39)	R244H	possibly damaging	Het
Asic5	A	T	3: 81,913,812 (GRCm39)	Y204F	probably benign	Het
Asz1	A	G	6: 18,104,876 (GRCm39)	F76S	probably damaging	Het
Bin1	G	A	18: 32,552,896 (GRCm39)	A174T	probably damaging	Het
Bnipl	A	C	3: 95,157,551 (GRCm39)	S23A	probably benign	Het
Cd19	T	C	7: 126,011,303 (GRCm39)	T294A	probably benign	Het
Cdon	G	A	9: 35,367,818 (GRCm39)	V238I	probably benign	Het
Chd3	A	T	11: 69,247,451 (GRCm39)	D957E	probably benign	Het
Crhbp	T	C	13: 95,568,515 (GRCm39)	Q307R	probably damaging	Het
Cst3	A	G	2: 148,714,741 (GRCm39)	M112T	probably benign	Het
Dgkb	A	G	12: 38,189,425 (GRCm39)	E276G	probably damaging	Het
Exoc4	T	A	6: 33,735,001 (GRCm39)	N539K	probably benign	Het
Gm3099	A	G	14: 15,345,429 (GRCm39)	E85G	probably benign	Het
Gpatch2	A	G	1: 186,957,698 (GRCm39)		probably null	Het
Hs3st5	T	A	10: 36,704,776 (GRCm39)	Y26*	probably null	Het
Hspg2	A	T	4: 137,244,148 (GRCm39)	D802V	probably damaging	Het
Ifih1	A	G	2: 62,431,813 (GRCm39)	V846A	possibly damaging	Het
Lama4	T	A	10: 38,964,840 (GRCm39)	S1402T	probably benign	Het
Lrmda	A	G	14: 22,648,388 (GRCm39)	T73A	unknown	Het
Lrrc1	A	T	9: 77,349,471 (GRCm39)	V365E	probably damaging	Het
Lrrc37a	T	A	11: 103,393,868 (GRCm39)	Q519L	probably benign	Het
Mlc1	G	T	15: 88,862,107 (GRCm39)	D36E	probably benign	Het
Mrc2	A	T	11: 105,223,092 (GRCm39)	D445V	probably damaging	Het
Muc16	A	G	9: 18,550,760 (GRCm39)	S5178P	probably benign	Het
Nbea	A	G	3: 55,573,110 (GRCm39)	I2491T	probably damaging	Het
Ncoa1	A	G	12: 4,389,044 (GRCm39)	I77T	probably damaging	Het
Npepps	G	T	11: 97,109,474 (GRCm39)	H701N	probably damaging	Het
Or4n4b	T	G	14: 50,536,041 (GRCm39)	T242P	probably damaging	Het
Or7g22	A	T	9: 19,048,831 (GRCm39)	I181F	possibly damaging	Het
Pclo	T	TTCTAG	5: 14,764,209 (GRCm39)		probably null	Het
Pclo	GTCTAT	GTCTATTCTAT	5: 14,764,204 (GRCm39)		probably null	Het
Pclo	TCTAT	TCTATACTAT	5: 14,764,205 (GRCm39)		probably null	Het
Pi4kb	T	C	3: 94,906,387 (GRCm39)	Y645H	probably damaging	Het
Pik3r5	C	T	11: 68,383,528 (GRCm39)	A449V	possibly damaging	Het
Plat	A	G	8: 23,261,736 (GRCm39)	T45A	probably benign	Het
Platr25	T	C	13: 62,848,676 (GRCm39)	K62R	possibly damaging	Het
Ppp5c	A	G	7: 16,740,111 (GRCm39)	V410A	possibly damaging	Het
Prdm2	C	T	4: 142,861,140 (GRCm39)	A717T	probably benign	Het
Pstpip2	C	A	18: 77,882,422 (GRCm39)	T2K	probably benign	Het
Ptpn12	A	C	5: 21,203,523 (GRCm39)	S418R	possibly damaging	Het
Rint1	A	G	5: 24,010,642 (GRCm39)	S255G	probably benign	Het
Rmc1	T	C	18: 12,322,371 (GRCm39)	L608P	probably damaging	Het
Sltm	C	G	9: 70,493,955 (GRCm39)	P802R	possibly damaging	Het
Stab2	T	C	10: 86,714,776 (GRCm39)	H1581R	probably benign	Het
Stau2	T	C	1: 16,530,577 (GRCm39)	Y114C	unknown	Het
Ticam1	T	C	17: 56,578,067 (GRCm39)	T343A	probably benign	Het
Tmem131l	T	C	3: 83,817,724 (GRCm39)	K1259E	possibly damaging	Het
Vmn2r103	T	A	17: 20,013,385 (GRCm39)	F169I	probably benign	Het
Vps33a	T	C	5: 123,673,312 (GRCm39)	K425E	possibly damaging	Het
Vwa8	T	C	14: 79,258,089 (GRCm39)	M746T	probably benign	Het
Zc3hav1	A	G	6: 38,313,598 (GRCm39)	I149T	possibly damaging	Het

Other mutations in Zfp708

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
P0024:Zfp708	UTSW	13	67,218,984 (GRCm39)	nonsense	probably null
R0611:Zfp708	UTSW	13	67,218,375 (GRCm39)	missense	probably benign	0.18
R0627:Zfp708	UTSW	13	67,218,781 (GRCm39)	nonsense	probably null
R1019:Zfp708	UTSW	13	67,222,162 (GRCm39)	missense	probably benign	0.05
R2230:Zfp708	UTSW	13	67,219,036 (GRCm39)	nonsense	probably null
R2512:Zfp708	UTSW	13	67,219,251 (GRCm39)	missense	probably damaging	1.00
R5718:Zfp708	UTSW	13	67,218,522 (GRCm39)	missense	probably benign	0.44
R6128:Zfp708	UTSW	13	67,222,965 (GRCm39)	missense	probably damaging	1.00
R6644:Zfp708	UTSW	13	67,218,785 (GRCm39)	missense	possibly damaging	0.88
R6720:Zfp708	UTSW	13	67,219,496 (GRCm39)	missense	probably damaging	1.00
R7082:Zfp708	UTSW	13	67,219,200 (GRCm39)	missense	possibly damaging	0.60
R9092:Zfp708	UTSW	13	67,218,564 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCAGCCACTGTAAATAAATGCAAAG -3'
(R):5'- GAGGTTCAACATCTGCAGGTTG -3'

Sequencing Primer
(F):5'- AGTAAGAGACTTCCACCAA -3'
(R):5'- CTGCAGGTTGTATATTACATCTAAGC -3'

Posted On

2019-12-20