Incidental Mutation 'R7887:Crcp'
Institutional Source Beutler Lab
Gene Symbol Crcp
Ensembl Gene ENSMUSG00000025532
Gene Namecalcitonin gene-related peptide-receptor component protein
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably essential (E-score: 0.871) question?
Stock #R7887 (G1)
Quality Score225.009
Status Validated
Chromosomal Location130029290-130060789 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to T at 130037870 bp
Amino Acid Change Lysine to Asparagine at position 32 (K32N)
Ref Sequence ENSEMBL: ENSMUSP00000026608 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000026608] [ENSMUST00000202163] [ENSMUST00000202756]
Predicted Effect possibly damaging
Transcript: ENSMUST00000026608
AA Change: K32N

PolyPhen 2 Score 0.937 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000026608
Gene: ENSMUSG00000025532
AA Change: K32N

RPOL4c 1 127 9.32e-62 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000202163
AA Change: K32N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000144624
Gene: ENSMUSG00000025532
AA Change: K32N

Blast:RPOL4c 1 47 2e-26 BLAST
low complexity region 48 59 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000202756
AA Change: K32N

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000143969
Gene: ENSMUSG00000025532
AA Change: K32N

RPOL4c 1 76 6.5e-8 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.2%
Validation Efficiency 98% (48/49)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a membrane protein that functions as part of a receptor complex for a small neuropeptide that increases intracellular cAMP levels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 50 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alkbh8 T A 9: 3,385,343 I580N probably damaging Het
Ankrd35 C T 3: 96,684,900 T834M probably damaging Het
Astn2 C T 4: 65,644,866 V893I possibly damaging Het
B4galt1 A G 4: 40,823,501 Y197H probably benign Het
Brdt T C 5: 107,359,933 S676P possibly damaging Het
Chrdl2 T C 7: 100,029,250 V343A possibly damaging Het
Clcn3 A T 8: 60,941,399 M59K probably benign Het
Cpne4 A T 9: 105,032,791 N529I probably damaging Het
Ddx54 C T 5: 120,627,203 R846C probably damaging Het
Dennd6a T C 14: 26,599,657 S118P possibly damaging Het
Egr3 A G 14: 70,079,202 Y116C probably damaging Het
Fbxw25 A G 9: 109,649,594 probably null Het
Focad T G 4: 88,182,616 I313M probably damaging Het
Gm10272 C T 10: 77,706,945 P107L probably benign Het
Gpr26 A C 7: 131,966,973 I16L probably benign Het
Gpr39 C A 1: 125,677,542 T69K probably damaging Het
Hacl1 C T 14: 31,634,227 G97S probably damaging Het
Idh3b A C 2: 130,281,758 D136E probably damaging Het
Irf6 G A 1: 193,167,732 V321M probably damaging Het
Klrg2 T A 6: 38,636,571 T166S probably damaging Het
Lnx2 T C 5: 147,019,043 I648V probably damaging Het
Mecr A G 4: 131,860,866 probably null Het
Mnat1 T A 12: 73,188,191 S205T probably benign Het
Mpnd G A 17: 56,011,097 G204D probably benign Het
Myh7 C T 14: 54,983,662 E935K possibly damaging Het
Nid1 G T 13: 13,499,733 R899L possibly damaging Het
Nisch C T 14: 31,176,695 W664* probably null Het
Nudt6 C T 3: 37,412,380 V157I possibly damaging Het
Olfr1156 T C 2: 87,949,880 M118V probably damaging Het
Olfr573-ps1 A C 7: 102,942,151 L142R possibly damaging Het
Olfr949-ps1 A T 9: 39,364,879 M107L unknown Het
Onecut2 T A 18: 64,340,975 M180K possibly damaging Het
Parg T A 14: 32,217,662 D548E possibly damaging Het
Pclo GTCTAT GTCTATTCTAT 5: 14,714,190 probably null Het
Phf11d A G 14: 59,359,580 Y57H probably damaging Het
Prkaca T C 8: 83,986,895 V99A probably benign Het
Rnf144b T A 13: 47,239,811 C209S probably damaging Het
Scly G A 1: 91,300,641 probably null Het
Sphkap T C 1: 83,277,412 Y872C probably benign Het
Ssh1 C T 5: 113,961,349 probably null Het
Strap T G 6: 137,739,809 L129V possibly damaging Het
Sympk T C 7: 19,034,439 I111T possibly damaging Het
Tprn C A 2: 25,264,012 A442E probably damaging Het
Tsen34 T C 7: 3,694,708 L36P probably damaging Het
Ubr4 T C 4: 139,407,810 F818L probably damaging Het
Uggt1 T C 1: 36,208,034 Y294C probably damaging Het
Usp20 A G 2: 31,020,894 K862E probably benign Het
Vmn1r201 A T 13: 22,474,786 I57F probably damaging Het
Wdr64 C T 1: 175,785,545 A662V not run Het
Other mutations in Crcp
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02005:Crcp APN 5 130042233 missense probably benign 0.33
IGL02475:Crcp APN 5 130059858 utr 3 prime probably benign
R0329:Crcp UTSW 5 130042242 missense possibly damaging 0.50
R0899:Crcp UTSW 5 130059831 missense probably benign 0.00
R3942:Crcp UTSW 5 130034950 critical splice donor site probably null
R4828:Crcp UTSW 5 130059762 missense probably damaging 1.00
R6168:Crcp UTSW 5 130037896 missense probably damaging 1.00
R8672:Crcp UTSW 5 130042236 missense probably benign 0.01
Predicted Primers PCR Primer

Sequencing Primer
Posted On2019-12-20