Incidental Mutation 'R0134:Nanos3'
ID 60917
Institutional Source Beutler Lab
Gene Symbol Nanos3
Ensembl Gene ENSMUSG00000056155
Gene Name nanos C2HC-type zinc finger 3
Synonyms nos3
MMRRC Submission 038419-MU
Accession Numbers
Essential gene? Possibly essential (E-score: 0.736) question?
Stock # R0134 (G1)
Quality Score 225
Status Validated
Chromosome 8
Chromosomal Location 84173731-84185217 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) C to T at 84176134 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Arginine to Glutamine at position 133 (R133Q)
Ref Sequence ENSEMBL: ENSMUSP00000147775 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000070102] [ENSMUST00000093375] [ENSMUST00000118856] [ENSMUST00000210202]
AlphaFold P60324
Predicted Effect probably damaging
Transcript: ENSMUST00000070102
AA Change: R133Q

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000067385
Gene: ENSMUSG00000056155
AA Change: R133Q

DomainStartEndE-ValueType
low complexity region 32 54 N/A INTRINSIC
Pfam:zf-nanos 57 110 1.2e-28 PFAM
low complexity region 157 178 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000083549
Predicted Effect probably benign
Transcript: ENSMUST00000093375
SMART Domains Protein: ENSMUSP00000091067
Gene: ENSMUSG00000008129

DomainStartEndE-ValueType
low complexity region 226 241 N/A INTRINSIC
low complexity region 291 306 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000102383
Predicted Effect probably benign
Transcript: ENSMUST00000118856
SMART Domains Protein: ENSMUSP00000113651
Gene: ENSMUSG00000008129

DomainStartEndE-ValueType
Pfam:DUF4671 1 193 2.1e-62 PFAM
Pfam:DUF4671 181 600 7.3e-131 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000210202
AA Change: R133Q

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211482
Meta Mutation Damage Score 0.1597 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.2%
  • 10x: 96.4%
  • 20x: 93.3%
Validation Efficiency 100% (59/59)
MGI Phenotype PHENOTYPE: Homozygous inactivation of this locus results in both male and female infertility. Both male and female gonads are reduced in size and are devoid of germ cells, putatively due to impaired maintainence of primordial germ cells. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
0610009O20Rik G A 18: 38,261,264 (GRCm38) V505I probably benign Het
1110059E24Rik T C 19: 21,598,201 (GRCm38) probably benign Het
Abca16 T A 7: 120,540,155 (GRCm38) L1470Q probably damaging Het
Arhgap23 G T 11: 97,444,328 (GRCm38) V70L probably benign Het
AW549877 T C 15: 3,986,294 (GRCm38) K263E probably damaging Het
Bicd1 T C 6: 149,512,950 (GRCm38) I387T probably benign Het
Btbd9 C T 17: 30,274,942 (GRCm38) D492N possibly damaging Het
Cd59b G A 2: 104,078,941 (GRCm38) probably null Het
Ddx50 A T 10: 62,621,377 (GRCm38) probably benign Het
Dnlz T C 2: 26,351,368 (GRCm38) N116S probably damaging Het
Efcab14 T C 4: 115,740,531 (GRCm38) F108L probably damaging Het
Esyt2 A G 12: 116,367,710 (GRCm38) N736S probably damaging Het
Exoc4 A G 6: 33,971,946 (GRCm38) D908G possibly damaging Het
Garnl3 T C 2: 33,006,804 (GRCm38) T608A possibly damaging Het
Hdac2 T A 10: 36,989,184 (GRCm38) D131E probably benign Het
Hes1 T C 16: 30,067,250 (GRCm38) V224A probably damaging Het
Hps1 G T 19: 42,766,180 (GRCm38) Q277K probably damaging Het
Ighv15-2 T G 12: 114,565,037 (GRCm38) probably benign Het
Il3 A G 11: 54,265,680 (GRCm38) probably null Het
Itgae A C 11: 73,111,342 (GRCm38) M91L probably benign Het
Kctd21 T A 7: 97,348,091 (GRCm38) I257N probably benign Het
Kif16b A T 2: 142,672,375 (GRCm38) S1215T probably benign Het
Lhx9 A T 1: 138,838,679 (GRCm38) C124S probably damaging Het
Lipo4 A G 19: 33,501,606 (GRCm38) V278A probably benign Het
Lrp1b T C 2: 40,596,983 (GRCm38) E142G probably damaging Het
Macf1 T C 4: 123,432,843 (GRCm38) M2835V possibly damaging Het
Map9 G A 3: 82,359,983 (GRCm38) probably benign Het
Miox C T 15: 89,334,454 (GRCm38) probably benign Het
Mndal A T 1: 173,857,513 (GRCm38) probably benign Het
Nepn A T 10: 52,400,437 (GRCm38) T29S probably damaging Het
Nlgn1 C T 3: 25,435,925 (GRCm38) C546Y probably damaging Het
Olfr1055 T C 2: 86,347,728 (GRCm38) I13V possibly damaging Het
Olfr307 A G 7: 86,335,595 (GRCm38) I267T probably benign Het
Pdgfra A G 5: 75,166,511 (GRCm38) D123G probably damaging Het
Plekhn1 T C 4: 156,228,243 (GRCm38) R53G probably benign Het
Pnp2 T C 14: 50,963,177 (GRCm38) F100S probably damaging Het
Prickle1 A G 15: 93,510,777 (GRCm38) L47P possibly damaging Het
Ptar1 T A 19: 23,718,095 (GRCm38) C309S probably benign Het
Rxfp1 A G 3: 79,657,476 (GRCm38) S327P probably damaging Het
Siah2 A G 3: 58,676,115 (GRCm38) V250A probably damaging Het
Siglecg G A 7: 43,411,171 (GRCm38) G325D probably damaging Het
Slc10a7 T A 8: 78,697,158 (GRCm38) probably null Het
Slc9a1 A G 4: 133,420,605 (GRCm38) K645E probably benign Het
Smarca4 T C 9: 21,637,324 (GRCm38) L302P probably damaging Het
Smyd1 G T 6: 71,216,765 (GRCm38) T392N probably damaging Het
Snrnp40 C G 4: 130,378,043 (GRCm38) probably null Het
Tenm3 A T 8: 48,674,472 (GRCm38) L57Q probably damaging Het
Tep1 C T 14: 50,829,693 (GRCm38) V2269I possibly damaging Het
Tpd52l1 A G 10: 31,379,256 (GRCm38) S32P probably damaging Het
Tsfm A G 10: 127,022,929 (GRCm38) probably benign Het
Ttn C A 2: 76,793,130 (GRCm38) V15368L possibly damaging Het
Ttn T A 2: 76,710,124 (GRCm38) R34173W probably damaging Het
Vmn2r13 C A 5: 109,175,049 (GRCm38) V125L probably benign Het
Vps13b T C 15: 35,887,261 (GRCm38) I3272T probably benign Het
Zfp108 A G 7: 24,260,467 (GRCm38) H161R probably benign Het
Zfp518b A G 5: 38,674,659 (GRCm38) M1T probably null Het
Other mutations in Nanos3
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0047:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0048:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0089:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0090:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0102:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0103:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0145:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0189:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0190:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0226:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0344:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R0494:Nanos3 UTSW 8 84,176,134 (GRCm38) missense probably damaging 1.00
R8971:Nanos3 UTSW 8 84,176,186 (GRCm38) missense probably benign 0.14
R9128:Nanos3 UTSW 8 84,176,451 (GRCm38) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- CCCAGTGGCAAACAGACATGAAATG -3'
(R):5'- TGCAAACACAATGGCGAGTCCC -3'

Sequencing Primer
(F):5'- TCTCTACTACCCTGTGCAATAAAGG -3'
(R):5'- TCCCATGTCCTGAAGGATGAG -3'
Posted On 2013-07-24