Mutagenetix > Incidental Mutation

Incidental Mutation 'R7891:Zfp934'

609352

Institutional Source

Beutler Lab

Gene Symbol

Zfp934

Ensembl Gene

ENSMUSG00000074865

Gene Name

zinc finger protein 934

Synonyms

6720457D02Rik

MMRRC Submission

045943-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.125) question?

Stock #

R7891 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

62664609-62727170 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 62668003 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 63 (S63R)

Ref Sequence

ENSEMBL: ENSMUSP00000152181 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076195] [ENSMUST00000082203] [ENSMUST00000101547] [ENSMUST00000220648] [ENSMUST00000221250] [ENSMUST00000222429] [ENSMUST00000222746] [ENSMUST00000222852]

AlphaFold

K7N6H3

Predicted Effect

probably benign
Transcript: ENSMUST00000076195

SMART Domains

Protein: ENSMUSP00000075552
Gene: ENSMUSG00000055228

Domain	Start	End	E-Value	Type
KRAB	4	66	8.26e-16	SMART
ZnF_C2H2	133	155	2.09e-3	SMART
ZnF_C2H2	161	183	1.4e-4	SMART
ZnF_C2H2	189	211	2.57e-3	SMART
ZnF_C2H2	217	239	5.5e-3	SMART
ZnF_C2H2	245	267	7.26e-3	SMART
ZnF_C2H2	273	295	1.4e-4	SMART
ZnF_C2H2	301	323	8.6e-5	SMART
ZnF_C2H2	329	351	1.2e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000082203
AA Change: S26R

PolyPhen 2 Score 0.079 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000080837
Gene: ENSMUSG00000074865
AA Change: S26R

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	3e-16	BLAST
ZnF_C2H2	101	123	1.04e-3	SMART
ZnF_C2H2	129	149	1.68e1	SMART
ZnF_C2H2	157	179	2.75e-3	SMART
ZnF_C2H2	185	207	1.98e-4	SMART
ZnF_C2H2	213	235	3.21e-4	SMART
ZnF_C2H2	241	263	3.44e-4	SMART
ZnF_C2H2	269	292	4.98e-1	SMART
ZnF_C2H2	298	320	4.87e-4	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000099083
Gene: ENSMUSG00000074865
AA Change: S58R

Domain	Start	End	E-Value	Type
KRAB	3	65	5.01e-15	SMART
ZnF_C2H2	132	154	1.04e-3	SMART
ZnF_C2H2	160	180	1.68e1	SMART
ZnF_C2H2	188	210	2.75e-3	SMART
ZnF_C2H2	216	238	1.98e-4	SMART
ZnF_C2H2	244	266	3.21e-4	SMART
ZnF_C2H2	272	294	3.44e-4	SMART
ZnF_C2H2	300	322	2.27e-4	SMART
ZnF_C2H2	328	350	3.44e-4	SMART
ZnF_C2H2	356	378	9.22e-5	SMART
ZnF_C2H2	384	406	2.43e-4	SMART
ZnF_C2H2	412	434	1.3e-4	SMART
ZnF_C2H2	440	462	4.87e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000220648
AA Change: S26R

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

Predicted Effect

probably benign
Transcript: ENSMUST00000221250

Predicted Effect

probably benign
Transcript: ENSMUST00000222429

Predicted Effect

probably benign
Transcript: ENSMUST00000222746

Predicted Effect

probably benign
Transcript: ENSMUST00000222852
AA Change: S63R

PolyPhen 2 Score 0.353 (Sensitivity: 0.90; Specificity: 0.89)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700025C18Rik	T	G	2: 164,920,864 (GRCm39)	E56D	unknown	Het
9930111J21Rik2	T	C	11: 48,910,543 (GRCm39)	Q630R	probably benign	Het
Abca9	A	G	11: 110,054,098 (GRCm39)	V68A	probably benign	Het
Acnat1	G	A	4: 49,449,181 (GRCm39)	A195V	possibly damaging	Het
Acsl3	C	T	1: 78,681,305 (GRCm39)	A612V	probably benign	Het
Adam12	A	T	7: 133,599,961 (GRCm39)	D115E	probably benign	Het
Adam3	T	A	8: 25,197,513 (GRCm39)		probably null	Het
Aif1	G	A	17: 35,391,600 (GRCm39)		probably benign	Het
Ank3	A	G	10: 69,824,139 (GRCm39)	D154G	probably damaging	Het
Arid3b	A	G	9: 57,717,442 (GRCm39)	C233R	probably benign	Het
Ccdc7b	T	A	8: 129,799,146 (GRCm39)	D74E	unknown	Het
Cckbr	T	A	7: 105,084,557 (GRCm39)	D430E	probably benign	Het
Chpf	T	C	1: 75,451,939 (GRCm39)	H667R	probably benign	Het
Dnah8	A	G	17: 30,931,263 (GRCm39)	E1426G	probably benign	Het
Dync1h1	A	G	12: 110,609,590 (GRCm39)	I2791V	probably benign	Het
Ehd3	A	G	17: 74,123,387 (GRCm39)	N130S	probably benign	Het
Fgfr4	A	G	13: 55,306,964 (GRCm39)	T218A	probably benign	Het
Foxe1	G	A	4: 46,344,599 (GRCm39)	E136K	possibly damaging	Het
Frmpd1	A	G	4: 45,284,478 (GRCm39)	S1100G	probably benign	Het
Gask1b	G	A	3: 79,793,591 (GRCm39)	A20T	probably benign	Het
Gm21886	T	G	18: 80,132,972 (GRCm39)	Q62P	probably null	Het
Golga4	A	G	9: 118,385,434 (GRCm39)	E852G	probably damaging	Het
Gprin3	T	C	6: 59,330,696 (GRCm39)	D537G	probably benign	Het
Gpx5	C	A	13: 21,472,918 (GRCm39)	D139Y	probably damaging	Het
Hmcn1	T	C	1: 150,468,940 (GRCm39)	Y5007C	probably damaging	Het
Igsf10	G	A	3: 59,235,832 (GRCm39)	R1450*	probably null	Het
Kif21a	G	A	15: 90,840,517 (GRCm39)	P1200S	probably damaging	Het
Kmt2c	G	A	5: 25,505,109 (GRCm39)	R3400C	probably damaging	Het
Miox	A	C	15: 89,220,742 (GRCm39)	M216L	probably benign	Het
Mphosph9	T	C	5: 124,428,967 (GRCm39)	Y687C	probably damaging	Het
Mxi1	T	A	19: 53,299,192 (GRCm39)	V21D	probably benign	Het
Nbeal1	T	C	1: 60,299,591 (GRCm39)	L1309P	probably benign	Het
Onecut2	T	A	18: 64,474,046 (GRCm39)	M180K	possibly damaging	Het
Or10ag54	A	T	2: 87,099,421 (GRCm39)	T99S	possibly damaging	Het
Or13c25	C	T	4: 52,911,663 (GRCm39)	V44I	probably benign	Het
Or4c100	G	A	2: 88,356,289 (GRCm39)	V121I	probably benign	Het
Or7g22	T	C	9: 19,049,141 (GRCm39)	M284T	possibly damaging	Het
Polq	A	G	16: 36,848,244 (GRCm39)	T284A	probably damaging	Het
Polrmt	A	T	10: 79,577,714 (GRCm39)	M295K	probably damaging	Het
Pou2af1	G	T	9: 51,144,297 (GRCm39)	M70I	probably damaging	Het
Pou3f1	G	A	4: 124,552,232 (GRCm39)	E245K	probably damaging	Het
Psmb1	C	T	17: 15,714,748 (GRCm39)	V50I	probably benign	Het
Ptgis	T	C	2: 167,069,434 (GRCm39)	D50G	probably damaging	Het
R3hdm2	A	G	10: 127,334,443 (GRCm39)	I955M	probably benign	Het
Rasef	G	A	4: 73,677,935 (GRCm39)	T97I	probably benign	Het
Rasef	T	C	4: 73,709,201 (GRCm39)	T11A	probably benign	Het
Sema4c	C	A	1: 36,588,995 (GRCm39)	L710F	probably damaging	Het
Slc39a10	C	T	1: 46,851,328 (GRCm39)	A721T	probably damaging	Het
Sltm	C	G	9: 70,493,955 (GRCm39)	P802R	possibly damaging	Het
Spata31d1a	A	G	13: 59,848,139 (GRCm39)	C1330R	possibly damaging	Het
Susd1	T	C	4: 59,349,915 (GRCm39)	D560G	possibly damaging	Het
Tnfrsf1b	T	C	4: 144,955,660 (GRCm39)	Y32C	probably damaging	Het
Trhr2	C	A	8: 123,084,083 (GRCm39)	V306F	probably damaging	Het
Trpm6	A	T	19: 18,754,074 (GRCm39)	Q35L	probably benign	Het
Ttbk2	A	G	2: 120,616,510 (GRCm39)	S197P	probably damaging	Het
Uggt2	T	A	14: 119,280,059 (GRCm39)	E806D	probably benign	Het
Ushbp1	T	G	8: 71,841,422 (GRCm39)	Q469P	possibly damaging	Het
Vmn1r73	G	A	7: 11,491,036 (GRCm39)	V285I	possibly damaging	Het

Other mutations in Zfp934

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1915:Zfp934	UTSW	13	62,665,769 (GRCm39)	missense	probably damaging	1.00
R1957:Zfp934	UTSW	13	62,666,108 (GRCm39)	missense	possibly damaging	0.83
R2113:Zfp934	UTSW	13	62,666,507 (GRCm39)	missense	probably damaging	0.98
R2145:Zfp934	UTSW	13	62,665,648 (GRCm39)	missense	probably damaging	1.00
R3732:Zfp934	UTSW	13	62,665,599 (GRCm39)	missense	probably damaging	1.00
R3797:Zfp934	UTSW	13	62,665,702 (GRCm39)	missense	probably benign
R4326:Zfp934	UTSW	13	62,665,373 (GRCm39)	missense	probably benign	0.01
R4327:Zfp934	UTSW	13	62,665,373 (GRCm39)	missense	probably benign	0.01
R4909:Zfp934	UTSW	13	62,665,768 (GRCm39)	missense	probably damaging	1.00
R5236:Zfp934	UTSW	13	62,665,527 (GRCm39)	missense	probably damaging	1.00
R5237:Zfp934	UTSW	13	62,665,652 (GRCm39)	missense	probably damaging	1.00
R6305:Zfp934	UTSW	13	62,666,370 (GRCm39)	missense	probably damaging	0.99
R6729:Zfp934	UTSW	13	62,640,746 (GRCm39)	missense	probably damaging	1.00
R7072:Zfp934	UTSW	13	62,668,339 (GRCm39)	missense	probably damaging	0.99
R7186:Zfp934	UTSW	13	62,640,204 (GRCm39)	missense	probably benign	0.12
R7235:Zfp934	UTSW	13	62,665,964 (GRCm39)	missense
R7453:Zfp934	UTSW	13	62,666,517 (GRCm39)	missense	probably benign	0.40
R7552:Zfp934	UTSW	13	62,640,705 (GRCm39)	missense	probably damaging	1.00
R7589:Zfp934	UTSW	13	62,666,130 (GRCm39)	missense
R7624:Zfp934	UTSW	13	62,666,338 (GRCm39)	missense	probably benign	0.27
R7651:Zfp934	UTSW	13	62,666,327 (GRCm39)	missense	probably benign
R7780:Zfp934	UTSW	13	62,666,358 (GRCm39)	missense	possibly damaging	0.92
R7857:Zfp934	UTSW	13	62,665,361 (GRCm39)	missense
R8130:Zfp934	UTSW	13	62,667,985 (GRCm39)	critical splice donor site	probably null
R8298:Zfp934	UTSW	13	62,666,295 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACTAAGGCATTGTTTCCTTGTCC -3'
(R):5'- AGAAGAAGCTCTACAAACATGTCA -3'

Sequencing Primer
(F):5'- AAGCTGTACTAATTTACACTGCTCC -3'
(R):5'- GCTCTACAAACATGTCATGCTGGAG -3'

Posted On

2019-12-20