Mutagenetix > Incidental Mutation

Incidental Mutation 'R7901:Or4m1'

610011

Institutional Source

Beutler Lab

Gene Symbol

Or4m1

Ensembl Gene

ENSMUSG00000045306

Gene Name

olfactory receptor family 4 subfamily M member 1

Synonyms

Olfr734, GA_x6K02T2PMLR-6013665-6012724, MOR242-1

MMRRC Submission

045953-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.495) question?

Stock #

R7901 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

50557293-50558325 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 50557573 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 240 (S240T)

Ref Sequence

ENSEMBL: ENSMUSP00000150732 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050928] [ENSMUST00000217152]

AlphaFold

Q8VFT4

Predicted Effect

probably damaging
Transcript: ENSMUST00000050928
AA Change: S240T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000057376
Gene: ENSMUSG00000045306
AA Change: S240T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.3e-39	PFAM
Pfam:7tm_1	41	302	4.1e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000217152
AA Change: S240T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	A	T	19: 57,119,434 (GRCm39)		probably null	Het
Adpgk	T	C	9: 59,222,300 (GRCm39)	V409A	probably benign	Het
Agfg2	A	T	5: 137,665,966 (GRCm39)	F98I	probably damaging	Het
Arhgap17	A	T	7: 122,885,791 (GRCm39)		probably benign	Het
Asb8	T	C	15: 98,040,614 (GRCm39)	Y16C	probably damaging	Het
Atp6v0a2	C	T	5: 124,779,485 (GRCm39)	T200I	probably damaging	Het
C1ra	G	A	6: 124,494,684 (GRCm39)	E316K	probably benign	Het
Ccnt1	A	T	15: 98,441,797 (GRCm39)	D490E	probably benign	Het
Cenpf	G	T	1: 189,389,445 (GRCm39)	D1462E	probably damaging	Het
Clcn6	C	T	4: 148,095,202 (GRCm39)	R736H	probably damaging	Het
Col4a2	A	T	8: 11,479,358 (GRCm39)	D747V	probably benign	Het
Cplane1	A	G	15: 8,299,190 (GRCm39)	E3126G	unknown	Het
Crybg3	T	C	16: 59,377,907 (GRCm39)	T1116A	probably damaging	Het
Csf1r	T	A	18: 61,243,368 (GRCm39)	L128Q	probably damaging	Het
Ddx17	A	T	15: 79,422,789 (GRCm39)	D316E	probably damaging	Het
Ect2l	T	A	10: 18,017,712 (GRCm39)	D681V	possibly damaging	Het
Ell2	A	T	13: 75,912,105 (GRCm39)	K464*	probably null	Het
Eme1	G	T	11: 94,541,645 (GRCm39)	P59Q	probably damaging	Het
Etl4	T	C	2: 20,294,821 (GRCm39)	S2P	possibly damaging	Het
Fndc9	T	C	11: 46,128,576 (GRCm39)	Y32H	probably damaging	Het
Frem1	G	T	4: 82,877,614 (GRCm39)	T1321K	probably benign	Het
Gabrg2	A	G	11: 41,867,418 (GRCm39)	V67A	probably benign	Het
Gm4553	ACAGCAGCTGGACTGACAGCAGCAGGGCTTGCAACAGCTGGACTGGCAGCAGCAGGGCTTGCAGCAGCTGGACTGGCAGCAGCAGGGCTTGCAGCAGCTGGACTGGCAGCAG	ACAGCAGCTGGACTGGCAGCAGCAGGGCTTGCAGCAGCTGGACTGGCAGCAG	7: 141,718,602 (GRCm39)		probably benign	Het
Gm5145	A	C	17: 20,790,900 (GRCm39)	I93L	possibly damaging	Het
Gpld1	T	A	13: 25,146,758 (GRCm39)	V240E	probably damaging	Het
Gsc	T	A	12: 104,439,131 (GRCm39)	S82C	possibly damaging	Het
H2-T10	C	T	17: 36,431,143 (GRCm39)	E173K	probably benign	Het
Ifi213	A	T	1: 173,394,784 (GRCm39)	S584T	probably benign	Het
Klk1b1	T	C	7: 43,620,669 (GRCm39)	I253T	probably damaging	Het
Klra4	A	G	6: 130,040,113 (GRCm39)	L53P	probably damaging	Het
Lct	A	G	1: 128,216,722 (GRCm39)	Y1697H	probably benign	Het
Mrs2	T	A	13: 25,202,549 (GRCm39)	D64V	possibly damaging	Het
Muc13	A	G	16: 33,636,211 (GRCm39)	Q565R	probably damaging	Het
Nlrp6	T	C	7: 140,507,353 (GRCm39)	V873A	possibly damaging	Het
Nup155	C	T	15: 8,145,926 (GRCm39)	P159L	possibly damaging	Het
Or10v1	A	T	19: 11,873,898 (GRCm39)	H171L	probably benign	Het
Or51a6	T	C	7: 102,604,887 (GRCm39)		probably null	Het
Phip	T	G	9: 82,772,203 (GRCm39)	M1115L	probably benign	Het
Ppip5k1	T	C	2: 121,142,390 (GRCm39)	Q1353R	probably damaging	Het
Ptprb	C	G	10: 116,205,333 (GRCm39)	P1896A	probably benign	Het
Rgl2	T	C	17: 34,154,799 (GRCm39)	L601P	possibly damaging	Het
Sash1	C	T	10: 8,656,328 (GRCm39)	W221*	probably null	Het
Skint1	A	C	4: 111,876,399 (GRCm39)	T107P	probably damaging	Het
Slc45a4	G	A	15: 73,477,621 (GRCm39)		probably benign	Het
Snx13	T	A	12: 35,150,624 (GRCm39)	D309E	probably benign	Het
Spcs1	A	G	14: 30,722,628 (GRCm39)	Y64H	probably benign	Het
Tep1	A	T	14: 51,064,308 (GRCm39)	Y2434N	possibly damaging	Het
Tnpo3	T	C	6: 29,568,990 (GRCm39)	E454G	possibly damaging	Het
Tsg101	G	T	7: 46,563,183 (GRCm39)	Q24K	probably benign	Het
Ttc6	C	T	12: 57,735,353 (GRCm39)	R1132W	probably damaging	Het
Unkl	T	C	17: 25,437,627 (GRCm39)	S200P	probably damaging	Het
Uts2r	G	A	11: 121,052,234 (GRCm39)	S366N	probably benign	Het
Wbp4	A	T	14: 79,709,845 (GRCm39)	V130E	probably damaging	Het
Zfp592	T	G	7: 80,674,469 (GRCm39)	S478A	probably benign	Het
Zfp664	A	T	5: 124,962,839 (GRCm39)	K78*	probably null	Het
Zfp738	A	T	13: 67,821,110 (GRCm39)	L79*	probably null	Het
Zfyve28	C	T	5: 34,382,326 (GRCm39)	R258Q	probably damaging	Het

Other mutations in Or4m1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01140:Or4m1	APN	14	50,557,732 (GRCm39)	missense	probably damaging	0.96
IGL01285:Or4m1	APN	14	50,557,713 (GRCm39)	missense	possibly damaging	0.88
IGL02106:Or4m1	APN	14	50,557,617 (GRCm39)	missense	probably damaging	1.00
IGL02313:Or4m1	APN	14	50,557,473 (GRCm39)	missense	probably damaging	0.99
IGL03125:Or4m1	APN	14	50,558,149 (GRCm39)	missense	probably benign	0.01
R0276:Or4m1	UTSW	14	50,557,636 (GRCm39)	missense	probably benign	0.23
R0547:Or4m1	UTSW	14	50,557,575 (GRCm39)	missense	probably benign	0.06
R0567:Or4m1	UTSW	14	50,558,115 (GRCm39)	missense	probably damaging	0.99
R0927:Or4m1	UTSW	14	50,558,186 (GRCm39)	nonsense	probably null
R1506:Or4m1	UTSW	14	50,557,941 (GRCm39)	missense	probably benign	0.00
R4032:Or4m1	UTSW	14	50,557,767 (GRCm39)	missense	possibly damaging	0.91
R5179:Or4m1	UTSW	14	50,557,993 (GRCm39)	nonsense	probably null
R5401:Or4m1	UTSW	14	50,557,566 (GRCm39)	missense	probably damaging	1.00
R6240:Or4m1	UTSW	14	50,558,043 (GRCm39)	missense	probably benign	0.00
R7752:Or4m1	UTSW	14	50,557,573 (GRCm39)	missense	probably damaging	1.00
R8034:Or4m1	UTSW	14	50,558,023 (GRCm39)	missense	probably damaging	1.00
R8260:Or4m1	UTSW	14	50,557,615 (GRCm39)	missense	probably benign	0.09
R8420:Or4m1	UTSW	14	50,558,233 (GRCm39)	missense	probably benign
R9056:Or4m1	UTSW	14	50,557,999 (GRCm39)	missense	probably damaging	0.98
R9128:Or4m1	UTSW	14	50,558,214 (GRCm39)	missense	probably benign	0.08
R9618:Or4m1	UTSW	14	50,557,760 (GRCm39)	nonsense	probably null
R9659:Or4m1	UTSW	14	50,558,181 (GRCm39)	missense	probably benign	0.10
R9788:Or4m1	UTSW	14	50,558,181 (GRCm39)	missense	probably benign	0.10
X0064:Or4m1	UTSW	14	50,557,511 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCACCACTTCCTGTGTTAGAAC -3'
(R):5'- TCACTCAGGTTGTCCGGATTG -3'

Sequencing Primer
(F):5'- ATATCTATTCACCAGCTTTCTCATGG -3'
(R):5'- CGGATTGCTTGTGCCAATAC -3'

Posted On

2019-12-20