Mutagenetix > Incidental Mutation

Incidental Mutation 'R7908:Olfr205'

610314

Institutional Source

Beutler Lab

Gene Symbol

Olfr205

Ensembl Gene

ENSMUSG00000094422

Gene Name

olfactory receptor 205

Synonyms

MOR182-11P, GA_x54KRFPKG5P-55543875-55542958

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.129) question?

Stock #

R7908 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

59328005-59331138 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 59329243 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 89 (D89N)

Ref Sequence

ENSEMBL: ENSMUSP00000149415 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074125] [ENSMUST00000213910]

AlphaFold

Q7TS37

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074125
AA Change: D89N

PolyPhen 2 Score 0.479 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000073762
Gene: ENSMUSG00000094422
AA Change: D89N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	305	1.6e-49	PFAM
Pfam:7TM_GPCR_Srsx	34	302	1.6e-7	PFAM
Pfam:7tm_1	40	289	7.8e-21	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213910
AA Change: D89N

PolyPhen 2 Score 0.479 (Sensitivity: 0.89; Specificity: 0.90)

Meta Mutation Damage Score

0.1712

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	A	T	12: 55,379,465	K533N	possibly damaging	Het
1110032A03Rik	T	A	9: 50,763,948	T153S	possibly damaging	Het
9530053A07Rik	A	T	7: 28,147,496	Y1168F	probably benign	Het
A4galt	A	G	15: 83,228,376	F69L	probably benign	Het
Acsf3	A	G	8: 122,785,823	M328V	probably damaging	Het
Adam10	T	G	9: 70,761,764	V454G	possibly damaging	Het
Adamts15	T	C	9: 30,902,226	D881G	probably benign	Het
Adamtsl1	T	C	4: 86,356,439	V1244A	probably benign	Het
Ankfn1	G	A	11: 89,405,534	P123L	probably damaging	Het
Aox1	T	C	1: 58,106,068	I1331T	possibly damaging	Het
Arntl	A	T	7: 113,313,473	I579L	probably benign	Het
Atg14	T	A	14: 47,568,593		probably benign	Het
Btnl4	A	G	17: 34,473,187	M147T	possibly damaging	Het
Camk1g	T	C	1: 193,359,774	K56E	probably damaging	Het
Capn7	T	C	14: 31,366,245		probably null	Het
Cbln1	T	A	8: 87,472,096	T49S	probably benign	Het
Cc2d2a	T	C	5: 43,706,846	V763A	probably benign	Het
Cdc40	A	G	10: 40,848,046	Y249H	probably damaging	Het
Cfap61	T	A	2: 146,102,099	L762H	probably damaging	Het
Cfap73	T	C	5: 120,630,058	T212A	probably benign	Het
Cfi	A	G	3: 129,848,584	E29G	probably benign	Het
Chmp6	A	T	11: 119,913,830		probably benign	Het
Clec14a	T	C	12: 58,267,679	T386A	possibly damaging	Het
Coq6	T	A	12: 84,371,166	L268H	probably damaging	Het
Cpt1a	T	C	19: 3,362,202	S225P	probably benign	Het
Dhx58	C	A	11: 100,695,304	L630F	probably damaging	Het
Dlg2	T	A	7: 91,900,773	V158E	probably damaging	Het
Foxd3	A	G	4: 99,657,339	T239A	probably benign	Het
Galnt6	T	C	15: 100,703,361	T346A	probably damaging	Het
Guca1b	C	G	17: 47,391,177	T185S	unknown	Het
H2-M5	T	C	17: 36,987,679	T292A	probably benign	Het
Hlx	T	C	1: 184,727,576	H455R	probably benign	Het
Hydin	A	G	8: 110,510,867	Y1924C	probably benign	Het
Ighg2b	C	T	12: 113,306,454	V315M		Het
Ildr2	A	G	1: 166,307,800	K374E	probably damaging	Het
Jmjd1c	T	C	10: 67,225,842	S1144P	probably benign	Het
Kcnj8	T	C	6: 142,566,029	D284G	probably benign	Het
Kri1	TTCCTCCTCCTCCTCCTCCTCCTCCTCCTC	TTCCTCCTCCTCCTCCTCCTCCTCCTC	9: 21,281,056		probably benign	Het
Lingo4	A	G	3: 94,402,234	T160A	probably benign	Het
Lmod3	G	T	6: 97,248,473	A129E	probably benign	Het
Lrrk2	T	C	15: 91,726,152	C696R	probably damaging	Het
Mc2r	A	G	18: 68,407,965	F86L	probably benign	Het
Mfsd7a	T	C	5: 108,444,497	I276V	probably benign	Het
Mppe1	T	G	18: 67,228,984	K170T	probably benign	Het
Mtcl1	A	G	17: 66,371,330	I667T	possibly damaging	Het
Mtnr1a	A	T	8: 45,087,826	I275F	probably benign	Het
Myh2	A	G	11: 67,197,371	I1938V	probably benign	Het
Npsr1	A	T	9: 24,289,800	E160V	probably damaging	Het
Olfr156	T	A	4: 43,821,086	I92F	probably damaging	Het
Olfr788	T	A	10: 129,472,998	I102N	probably damaging	Het
Ovch2	T	A	7: 107,789,119	Y418F	probably damaging	Het
Pak7	A	G	2: 136,116,559	L203S	probably benign	Het
Pcdh15	A	T	10: 74,643,582	E508D	probably benign	Het
Prl7a1	T	C	13: 27,642,450	M1V	probably null	Het
Prpsap2	G	T	11: 61,756,272	N14K	possibly damaging	Het
Rad54b	T	A	4: 11,595,868	V215E	probably null	Het
Ryr2	G	A	13: 11,792,748	T845I	probably benign	Het
Scgb2b3	G	A	7: 31,362,014	T20I	probably damaging	Het
Serpina1b	T	C	12: 103,728,307	I393V	possibly damaging	Het
Slc13a5	T	C	11: 72,259,064	M207V	probably benign	Het
Smg1	A	T	7: 118,186,134	L843M	unknown	Het
Sp100	T	C	1: 85,708,067	V531A	possibly damaging	Het
Srsf6	T	C	2: 162,933,840	S190P	unknown	Het
Stil	A	G	4: 115,032,699	K795E	possibly damaging	Het
Sugp2	A	G	8: 70,251,927	R705G	probably benign	Het
Tnfrsf11a	T	A	1: 105,809,374	C69S	probably damaging	Het
Trim30a	A	T	7: 104,421,449	N252K	probably benign	Het
Tshz1	T	A	18: 84,014,607	K559*	probably null	Het
Ttbk1	C	T	17: 46,478,938	R133H	probably damaging	Het
Vmn1r1	T	C	1: 182,157,350	Y250C	probably benign	Het
Vmn2r12	A	G	5: 109,086,441	L635P	probably damaging	Het
Vmn2r52	C	A	7: 10,162,950	V532L	probably benign	Het
Vwa5b1	A	T	4: 138,569,170	L1182*	probably null	Het
Washc2	A	G	6: 116,248,145	D818G	probably benign	Het
Xylb	T	G	9: 119,381,545	S365A	probably benign	Het
Zcchc8	T	C	5: 123,720,720		probably benign	Het
Zdbf2	T	C	1: 63,306,827	I1455T	possibly damaging	Het

Other mutations in Olfr205

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02475:Olfr205	APN	16	59,328,725 (GRCm38)	missense	probably benign	0.21
IGL03236:Olfr205	APN	16	59,328,837 (GRCm38)	missense	probably damaging	0.97
R0054:Olfr205	UTSW	16	59,329,065 (GRCm38)	missense	possibly damaging	0.57
R0054:Olfr205	UTSW	16	59,329,065 (GRCm38)	missense	possibly damaging	0.57
R0167:Olfr205	UTSW	16	59,328,974 (GRCm38)	nonsense	probably null
R0178:Olfr205	UTSW	16	59,329,420 (GRCm38)	missense	probably damaging	1.00
R0371:Olfr205	UTSW	16	59,329,222 (GRCm38)	missense	possibly damaging	0.60
R0577:Olfr205	UTSW	16	59,328,698 (GRCm38)	missense	probably benign	0.01
R0597:Olfr205	UTSW	16	59,328,760 (GRCm38)	missense	probably damaging	1.00
R0967:Olfr205	UTSW	16	59,329,183 (GRCm38)	missense	possibly damaging	0.66
R1670:Olfr205	UTSW	16	59,329,244 (GRCm38)	missense	probably benign	0.03
R1702:Olfr205	UTSW	16	59,329,141 (GRCm38)	missense	probably benign	0.12
R1995:Olfr205	UTSW	16	59,329,291 (GRCm38)	missense	probably damaging	1.00
R2239:Olfr205	UTSW	16	59,329,375 (GRCm38)	missense	probably damaging	0.99
R4063:Olfr205	UTSW	16	59,328,880 (GRCm38)	missense	probably benign	0.05
R4400:Olfr205	UTSW	16	59,328,598 (GRCm38)	missense	probably benign
R4666:Olfr205	UTSW	16	59,329,210 (GRCm38)	missense	possibly damaging	0.91
R4795:Olfr205	UTSW	16	59,328,850 (GRCm38)	missense	probably benign	0.09
R5327:Olfr205	UTSW	16	59,329,098 (GRCm38)	missense	probably benign	0.01
R5471:Olfr205	UTSW	16	59,328,631 (GRCm38)	missense	probably damaging	1.00
R5770:Olfr205	UTSW	16	59,329,151 (GRCm38)	nonsense	probably null
R6195:Olfr205	UTSW	16	59,329,422 (GRCm38)	missense	possibly damaging	0.81
R6702:Olfr205	UTSW	16	59,328,598 (GRCm38)	missense	probably benign
R7686:Olfr205	UTSW	16	59,329,016 (GRCm38)	missense	probably damaging	1.00
R7911:Olfr205	UTSW	16	59,329,243 (GRCm38)	missense	possibly damaging	0.48
R7912:Olfr205	UTSW	16	59,329,243 (GRCm38)	missense	possibly damaging	0.48
R7913:Olfr205	UTSW	16	59,329,243 (GRCm38)	missense	possibly damaging	0.48
R7998:Olfr205	UTSW	16	59,329,270 (GRCm38)	missense	probably benign	0.09
R8772:Olfr205	UTSW	16	59,328,688 (GRCm38)	missense	probably damaging	1.00
R9563:Olfr205	UTSW	16	59,329,402 (GRCm38)	missense	probably benign	0.00
X0026:Olfr205	UTSW	16	59,329,350 (GRCm38)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- CTGATGAACACAGTGCAGAGTC -3'
(R):5'- AGCACCTTGTTGACTGAGTTTG -3'

Sequencing Primer
(F):5'- ACACAGTGCAGAGTCTCTTG -3'
(R):5'- AGATCGTCCAGAGCTTCAAGTTC -3'

Posted On

2019-12-20