Incidental Mutation 'R7923:Zfp229'
ID611395
Institutional Source Beutler Lab
Gene Symbol Zfp229
Ensembl Gene ENSMUSG00000061544
Gene Namezinc finger protein 229
Synonyms
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.122) question?
Stock #R7923 (G1)
Quality Score225.009
Status Not validated
Chromosome17
Chromosomal Location21730795-21769342 bp(+) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) A to T at 21746150 bp
ZygosityHeterozygous
Amino Acid Change Lysine to Stop codon at position 454 (K454*)
Ref Sequence ENSEMBL: ENSMUSP00000138107 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000065871] [ENSMUST00000182603] [ENSMUST00000182827] [ENSMUST00000183192]
Predicted Effect probably null
Transcript: ENSMUST00000065871
AA Change: K454*
SMART Domains Protein: ENSMUSP00000065379
Gene: ENSMUSG00000061544
AA Change: K454*

DomainStartEndE-ValueType
KRAB 13 73 7.74e-30 SMART
ZnF_C2H2 229 249 1.88e2 SMART
ZnF_C2H2 257 279 1.38e-3 SMART
ZnF_C2H2 285 307 3.63e-3 SMART
ZnF_C2H2 312 334 9.44e-2 SMART
ZnF_C2H2 340 362 3.58e-2 SMART
ZnF_C2H2 368 390 2.99e-4 SMART
ZnF_C2H2 395 417 2.36e-2 SMART
ZnF_C2H2 423 445 2.09e-3 SMART
ZnF_C2H2 451 473 1.18e-2 SMART
ZnF_C2H2 479 501 6.78e-3 SMART
ZnF_C2H2 507 529 1.28e-3 SMART
ZnF_C2H2 535 557 7.05e-1 SMART
ZnF_C2H2 562 584 8.02e-5 SMART
ZnF_C2H2 590 612 9.58e-3 SMART
ZnF_C2H2 618 640 7.78e-3 SMART
ZnF_C2H2 646 668 3.83e-2 SMART
ZnF_C2H2 674 696 1.72e-4 SMART
Predicted Effect probably null
Transcript: ENSMUST00000182603
AA Change: K454*
SMART Domains Protein: ENSMUSP00000138107
Gene: ENSMUSG00000061544
AA Change: K454*

DomainStartEndE-ValueType
KRAB 13 73 7.74e-30 SMART
Pfam:zf-C2H2_6 205 219 3.9e-1 PFAM
ZnF_C2H2 229 249 1.88e2 SMART
ZnF_C2H2 257 279 1.38e-3 SMART
ZnF_C2H2 285 307 3.63e-3 SMART
ZnF_C2H2 312 334 9.44e-2 SMART
ZnF_C2H2 340 362 3.58e-2 SMART
ZnF_C2H2 368 390 2.99e-4 SMART
ZnF_C2H2 395 417 2.36e-2 SMART
ZnF_C2H2 423 445 2.09e-3 SMART
ZnF_C2H2 451 473 1.18e-2 SMART
ZnF_C2H2 479 501 6.78e-3 SMART
ZnF_C2H2 507 529 1.28e-3 SMART
ZnF_C2H2 535 557 7.05e-1 SMART
ZnF_C2H2 562 584 8.02e-5 SMART
ZnF_C2H2 590 612 9.58e-3 SMART
ZnF_C2H2 618 640 7.78e-3 SMART
ZnF_C2H2 646 668 3.83e-2 SMART
ZnF_C2H2 674 696 1.72e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000182827
SMART Domains Protein: ENSMUSP00000138478
Gene: ENSMUSG00000061544

DomainStartEndE-ValueType
KRAB 13 73 7.74e-30 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000183192
SMART Domains Protein: ENSMUSP00000138265
Gene: ENSMUSG00000061544

DomainStartEndE-ValueType
KRAB 13 73 7.74e-30 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.5%
  • 20x: 98.4%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 72 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110034G24Rik A G 2: 132,750,590 T186A probably damaging Het
Abca16 A G 7: 120,475,466 N577S probably benign Het
Ang4 A G 14: 51,764,072 S140P probably damaging Het
Ankdd1b T C 13: 96,419,798 probably null Het
Anln G T 9: 22,362,723 A606D probably benign Het
Ano5 T G 7: 51,587,732 V768G possibly damaging Het
Ccdc180 A T 4: 45,900,461 Y275F possibly damaging Het
Chpf T C 1: 75,476,627 Y334C probably damaging Het
Chrdl2 T C 7: 100,033,656 L381S probably damaging Het
Cox20 A T 1: 178,322,561 Y100F probably benign Het
Dbn1 T C 13: 55,475,509 D433G possibly damaging Het
Dclre1a A T 19: 56,531,252 I983N probably damaging Het
Dok6 A T 18: 89,560,058 H78Q probably benign Het
Fam35a A G 14: 34,237,566 Y820H probably damaging Het
Fam92b T C 8: 120,166,633 D273G probably benign Het
Fpr1 A T 17: 17,877,372 F118L probably benign Het
Fscn3 A G 6: 28,430,176 Y115C probably damaging Het
Gabra4 A C 5: 71,640,913 probably null Het
Gm11639 G A 11: 104,733,713 D878N probably benign Het
Gm45861 T C 8: 27,582,723 V1290A unknown Het
Hivep1 T C 13: 42,155,352 V356A probably benign Het
Ikbip T C 10: 91,101,755 Y97H possibly damaging Het
Il10 A G 1: 131,020,068 T53A probably benign Het
Inpp5f T C 7: 128,694,802 V959A probably benign Het
Insr T C 8: 3,258,415 E207G probably benign Het
Kndc1 T A 7: 139,923,816 S1012T probably damaging Het
Ksr2 T C 5: 117,555,264 V259A probably benign Het
Lox A T 18: 52,525,122 Y326* probably null Het
Lpin2 C A 17: 71,230,274 T272N probably benign Het
Lrp2 C T 2: 69,464,784 E3397K probably damaging Het
Megf11 G A 9: 64,695,427 C850Y possibly damaging Het
Morc1 T C 16: 48,498,784 F284L probably benign Het
Moxd2 T A 6: 40,885,444 D147V possibly damaging Het
Mrpl21 T C 19: 3,287,752 L153P probably damaging Het
Naip2 T C 13: 100,144,409 T1432A probably benign Het
Naip6 T A 13: 100,315,471 D211V probably damaging Het
Ncoa5 T C 2: 165,012,896 H102R possibly damaging Het
Ndel1 A T 11: 68,829,980 C293* probably null Het
Ninl A T 2: 150,966,096 M82K probably benign Het
Nrcam G A 12: 44,541,075 probably null Het
Nup210l A T 3: 90,122,729 I250L probably benign Het
Obscn T C 11: 59,077,950 E2532G possibly damaging Het
Olfr1062 A G 2: 86,423,239 F146L probably benign Het
Olfr110 G T 17: 37,498,977 G109C probably damaging Het
Olfr20 C A 11: 73,353,759 A2E probably benign Het
Osgin1 G A 8: 119,445,034 S189N possibly damaging Het
Otud7b A G 3: 96,155,373 E643G probably damaging Het
Phldb2 T A 16: 45,751,364 T1222S probably damaging Het
Pwp1 C T 10: 85,888,050 R472W probably damaging Het
Rad21 T C 15: 51,973,142 Y211C probably damaging Het
Rexo1 A G 10: 80,550,738 V162A probably benign Het
Rtl1 C T 12: 109,594,155 V417I probably benign Het
Setbp1 G A 18: 78,783,424 P1325S probably benign Het
Skint7 G T 4: 111,982,226 C239F probably benign Het
Slc25a1 A G 16: 17,926,274 L221P probably benign Het
Snrnp70 T A 7: 45,376,790 D385V unknown Het
Sp7 A C 15: 102,359,098 V109G probably benign Het
Syne1 A T 10: 5,132,078 Y849N probably damaging Het
Tas2r108 T C 6: 40,493,856 F89L possibly damaging Het
Tcf3 C T 10: 80,410,467 A581T possibly damaging Het
Tcrg-C1 A T 13: 19,216,521 Y140F Het
Tcta A G 9: 108,305,787 L53P probably damaging Het
Traf3ip2 C G 10: 39,626,455 Q200E probably damaging Het
Tyk2 A T 9: 21,124,967 C75S probably damaging Het
Ugt3a1 G A 15: 9,311,817 G382R probably damaging Het
Upp2 G T 2: 58,774,115 probably null Het
Vmn1r212 A T 13: 22,883,153 F337I unknown Het
Vmn1r58 T C 7: 5,411,243 probably benign Het
Vps13d A T 4: 145,103,676 V2856D Het
Wdr37 A C 13: 8,836,875 L292R probably damaging Het
Yipf3 T C 17: 46,250,864 I199T probably benign Het
Zdhhc23 A G 16: 43,971,544 S289P possibly damaging Het
Other mutations in Zfp229
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00733:Zfp229 APN 17 21739502 missense possibly damaging 0.52
R0268:Zfp229 UTSW 17 21745841 missense probably benign
R0344:Zfp229 UTSW 17 21745841 missense probably benign
R0608:Zfp229 UTSW 17 21746634 missense probably damaging 1.00
R1672:Zfp229 UTSW 17 21745847 missense probably damaging 1.00
R2215:Zfp229 UTSW 17 21746277 missense possibly damaging 0.90
R2519:Zfp229 UTSW 17 21745587 missense possibly damaging 0.89
R2937:Zfp229 UTSW 17 21745503 missense probably damaging 1.00
R2965:Zfp229 UTSW 17 21746029 missense probably damaging 1.00
R3108:Zfp229 UTSW 17 21746816 missense probably damaging 1.00
R3768:Zfp229 UTSW 17 21745863 missense probably damaging 1.00
R3778:Zfp229 UTSW 17 21745202 missense probably benign 0.44
R3850:Zfp229 UTSW 17 21745862 missense probably damaging 1.00
R4273:Zfp229 UTSW 17 21746821 missense probably benign 0.02
R4731:Zfp229 UTSW 17 21745286 missense possibly damaging 0.73
R4732:Zfp229 UTSW 17 21745286 missense possibly damaging 0.73
R4733:Zfp229 UTSW 17 21745286 missense possibly damaging 0.73
R4818:Zfp229 UTSW 17 21743904 missense probably benign 0.33
R4929:Zfp229 UTSW 17 21746373 missense probably damaging 1.00
R5512:Zfp229 UTSW 17 21745036 splice site probably null
R7124:Zfp229 UTSW 17 21742616 missense probably damaging 0.98
R7511:Zfp229 UTSW 17 21745064 missense probably benign 0.00
R7576:Zfp229 UTSW 17 21745299 missense probably damaging 1.00
R7840:Zfp229 UTSW 17 21746150 nonsense probably null
Z1177:Zfp229 UTSW 17 21743942 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- TCAGAACATGCATGCTGGAAAACTA -3'
(R):5'- CCAGCATGAACTTTCTGATGTGT -3'

Sequencing Primer
(F):5'- TGCTGGAAAACTATATAAATGTCAGG -3'
(R):5'- TGTCTCCTACAAGTTGAGCAG -3'
Posted On2019-12-27