Incidental Mutation 'R0688:Apol10b'
ID61256
Institutional Source Beutler Lab
Gene Symbol Apol10b
Ensembl Gene ENSMUSG00000050014
Gene Nameapolipoprotein L 10B
Synonyms
MMRRC Submission 038873-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R0688 (G1)
Quality Score125
Status Not validated
Chromosome15
Chromosomal Location77583819-77596125 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 77585219 bp
ZygosityHeterozygous
Amino Acid Change Serine to Proline at position 253 (S253P)
Ref Sequence ENSEMBL: ENSMUSP00000086890 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000089465]
Predicted Effect probably damaging
Transcript: ENSMUST00000089465
AA Change: S253P

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000086890
Gene: ENSMUSG00000050014
AA Change: S253P

DomainStartEndE-ValueType
Pfam:ApoL 28 328 2.8e-88 PFAM
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.4%
  • 20x: 95.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acad11 T G 9: 104,124,100 V615G probably damaging Het
Apaf1 T G 10: 91,061,705 E305D possibly damaging Het
Bbs9 T A 9: 22,567,719 C153S probably damaging Het
Bicra C T 7: 15,989,322 G90D probably damaging Het
Clca4a A C 3: 144,961,974 L412R probably damaging Het
Cul3 A T 1: 80,271,564 D597E possibly damaging Het
Cxcr5 A G 9: 44,513,667 probably null Het
Dnah10 T C 5: 124,747,718 I646T possibly damaging Het
Focad T A 4: 88,274,213 V593D unknown Het
Fsip2 T A 2: 82,982,339 S3001T probably benign Het
Ganab T A 19: 8,911,113 Y511N probably damaging Het
Gdf9 T A 11: 53,436,640 L141Q probably damaging Het
Gm13083 T C 4: 143,617,357 F409S probably benign Het
Gm4450 T C 3: 98,456,394 E45G probably benign Het
Gpr180 A G 14: 118,148,184 D136G probably benign Het
Itga2 G T 13: 114,839,554 A1094E probably benign Het
Ly75 C T 2: 60,316,221 A1238T probably benign Het
Macc1 T C 12: 119,447,003 V502A probably damaging Het
Mroh4 T C 15: 74,606,678 K923E probably damaging Het
Msh3 G A 13: 92,351,431 P93S possibly damaging Het
Myo18a A G 11: 77,824,140 D474G probably damaging Het
Npat T A 9: 53,570,222 Y1077N probably benign Het
Olfr1214 A T 2: 88,987,595 S202R probably damaging Het
Olfr427 A C 1: 174,100,064 H202P probably damaging Het
Olfr52 A T 2: 86,181,605 probably null Het
Olfr816 T G 10: 129,911,883 T132P probably damaging Het
Paqr5 T G 9: 61,972,794 T59P probably benign Het
Phyhipl C T 10: 70,559,255 G329R probably damaging Het
Pomgnt1 T C 4: 116,155,889 Y430H probably damaging Het
Prkd3 A T 17: 78,957,233 M651K probably damaging Het
Puf60 A T 15: 76,070,774 M440K probably damaging Het
Recql4 A G 15: 76,709,809 probably null Het
Sgk1 A C 10: 21,998,160 M320L probably benign Het
Slc27a4 T C 2: 29,812,615 F509S probably damaging Het
Sorbs1 T C 19: 40,363,262 T235A probably damaging Het
Srrm3 A G 5: 135,869,276 probably benign Het
Tex15 C T 8: 33,573,500 T986I probably damaging Het
Ugcg C T 4: 59,207,798 P46S probably benign Het
Zswim4 T C 8: 84,228,888 M301V possibly damaging Het
Other mutations in Apol10b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01295:Apol10b APN 15 77585596 missense probably damaging 1.00
IGL01654:Apol10b APN 15 77588796 missense probably benign
IGL01905:Apol10b APN 15 77585359 missense possibly damaging 0.92
IGL01969:Apol10b APN 15 77588685 splice site probably null
IGL02305:Apol10b APN 15 77585430 missense possibly damaging 0.55
R0361:Apol10b UTSW 15 77585386 missense possibly damaging 0.82
R0395:Apol10b UTSW 15 77585640 missense probably damaging 1.00
R0437:Apol10b UTSW 15 77585408 missense probably benign 0.00
R0502:Apol10b UTSW 15 77592149 splice site probably benign
R1663:Apol10b UTSW 15 77588714 missense probably damaging 1.00
R1763:Apol10b UTSW 15 77585015 missense probably benign 0.14
R4884:Apol10b UTSW 15 77588806 missense possibly damaging 0.93
R6177:Apol10b UTSW 15 77585787 missense possibly damaging 0.50
R7062:Apol10b UTSW 15 77585273 missense probably benign 0.00
R7480:Apol10b UTSW 15 77588788 missense probably benign 0.02
R8511:Apol10b UTSW 15 77585010 missense probably benign 0.03
R8511:Apol10b UTSW 15 77585011 missense probably benign 0.43
Predicted Primers PCR Primer
(F):5'- ACTCTTAGCTGCTTAGAGGATGGGG -3'
(R):5'- CAGATGAATCTGAAGCCAGTCGCC -3'

Sequencing Primer
(F):5'- GCAGAGCCTTATATATCTGCTCG -3'
(R):5'- CTGGTTAGAGCCAGCATGAATG -3'
Posted On2013-07-30