Mutagenetix > Incidental Mutation

Incidental Mutation 'R7581:Olfr420'

615927

Institutional Source

Beutler Lab

Gene Symbol

Olfr420

Ensembl Gene

ENSMUSG00000055033

Gene Name

olfactory receptor 420

Synonyms

GA_x6K02T2P20D-20995211-20994246, MOR105-10

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

R7581 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

174155633-174162195 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 174158771 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000069662 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068403] [ENSMUST00000213748]

AlphaFold

E9Q4G0

Predicted Effect

probably null
Transcript: ENSMUST00000068403

SMART Domains

Protein: ENSMUSP00000069662
Gene: ENSMUSG00000055033

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	310	9.9e-60	PFAM
Pfam:7tm_1	41	292	4.1e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213748

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (47/48)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430403G16Rik	G	A	5: 109,690,788		probably benign	Het
Adamts9	A	C	6: 92,937,338	D196E	probably benign	Het
Adnp	A	T	2: 168,183,466	D636E	probably damaging	Het
Afdn	T	C	17: 13,849,238	V723A	probably damaging	Het
Arid1a	A	T	4: 133,680,351	F1897I	unknown	Het
Atp6v0b	T	C	4: 117,885,286	M137V	probably benign	Het
Bicd1	T	A	6: 149,519,004	I784N	probably damaging	Het
Cckbr	A	G	7: 105,433,786	T119A	probably benign	Het
Creb5	T	C	6: 53,681,237	L184P	probably damaging	Het
Cyp2a22	T	G	7: 26,938,148	K141Q	possibly damaging	Het
D16Ertd472e	T	C	16: 78,546,557	T219A	possibly damaging	Het
Ep400	A	T	5: 110,756,025	L236Q	unknown	Het
Exph5	A	G	9: 53,372,557	S313G	possibly damaging	Het
Gm10024	T	C	10: 77,711,563	V36A	unknown	Het
Gm5493	A	G	17: 22,747,274	E44G	probably damaging	Het
Gtdc1	A	T	2: 44,790,005		probably null	Het
Invs	T	C	4: 48,421,909	V847A	probably benign	Het
Kif18b	C	A	11: 102,914,722	Q236H	probably damaging	Het
Kntc1	A	G	5: 123,816,755	T2079A	probably benign	Het
Large2	A	G	2: 92,370,193	S89P	probably damaging	Het
Maml3	A	C	3: 51,856,768	D258E	probably benign	Het
Mdga2	A	T	12: 66,506,255	M868K	probably damaging	Het
Mtss1l	A	G	8: 110,726,213	E30G	possibly damaging	Het
Muc16	T	C	9: 18,645,614	I3128V	unknown	Het
Mug2	C	T	6: 122,063,711	T740I	probably damaging	Het
Noc2l	T	C	4: 156,245,449	V612A	probably benign	Het
Olfr979	A	T	9: 40,000,422	D268E	probably damaging	Het
Padi6	T	C	4: 140,728,929	T585A	probably benign	Het
Pcdhga1	A	T	18: 37,662,177	N78I	probably damaging	Het
Pdgfd	A	G	9: 6,293,894	Y156C	probably damaging	Het
Peg10	GCACATCAGGATCC	GCACATCAGGATCCCCATCAGGATCCTCCACATCAGGATCC	6: 4,756,452		probably benign	Het
Pi4ka	C	T	16: 17,301,060	V1307I		Het
Pip5k1c	A	G	10: 81,308,960	N212D	probably damaging	Het
Plekha1	A	G	7: 130,910,865	T264A	probably benign	Het
Polr2b	A	G	5: 77,326,704	R463G	probably damaging	Het
Psd2	T	G	18: 35,979,997	D248E	probably benign	Het
Rag1	G	T	2: 101,643,304	Q498K	possibly damaging	Het
Ryr3	A	G	2: 112,753,027	I2853T	probably damaging	Het
Selenow	G	T	7: 15,922,382		probably null	Het
Sned1	C	T	1: 93,256,545	S165F	probably benign	Het
Spata31d1a	A	G	13: 59,704,139		probably null	Het
Taar4	C	A	10: 23,961,154	H221N	probably damaging	Het
Tgm6	A	T	2: 130,141,285	R265W	probably damaging	Het
Trpm1	A	T	7: 64,204,555	Q275L	probably benign	Het
Ulk3	T	C	9: 57,592,042	L173P	probably damaging	Het
Urah	A	T	7: 140,835,627	T3S	probably benign	Het
Vmn1r149	A	T	7: 22,437,904	V109D	probably damaging	Het
Xpc	A	T	6: 91,498,017		probably benign	Het
Zglp1	T	A	9: 21,062,708	K227N	probably damaging	Het

Other mutations in Olfr420

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01394:Olfr420	APN	1	174158857	missense	probably damaging	1.00
IGL02479:Olfr420	APN	1	174158954	nonsense	probably null
IGL03190:Olfr420	APN	1	174159544	missense	probably damaging	0.99
IGL03270:Olfr420	APN	1	174159553	missense	probably benign	0.04
R0645:Olfr420	UTSW	1	174159354	missense	probably benign	0.00
R0834:Olfr420	UTSW	1	174159364	missense	possibly damaging	0.55
R1432:Olfr420	UTSW	1	174158917	missense	possibly damaging	0.67
R1508:Olfr420	UTSW	1	174159364	missense	possibly damaging	0.55
R2351:Olfr420	UTSW	1	174158920	missense	probably damaging	0.99
R3440:Olfr420	UTSW	1	174159180	missense	probably benign	0.14
R3441:Olfr420	UTSW	1	174159180	missense	probably benign	0.14
R4571:Olfr420	UTSW	1	174158928	missense	possibly damaging	0.77
R5072:Olfr420	UTSW	1	174158961	missense	probably damaging	1.00
R6060:Olfr420	UTSW	1	174159341	nonsense	probably null
R6166:Olfr420	UTSW	1	174159093	missense	probably benign	0.43
R6228:Olfr420	UTSW	1	174152146	missense	probably benign	0.00
R6272:Olfr420	UTSW	1	174159175	missense	probably benign	0.02
R6298:Olfr420	UTSW	1	174152182	missense	probably benign	0.02
R6400:Olfr420	UTSW	1	174159264	missense	probably damaging	0.99
R7677:Olfr420	UTSW	1	174159048	missense	probably damaging	1.00
R7823:Olfr420	UTSW	1	174159688	missense	probably benign	0.12
R7829:Olfr420	UTSW	1	174158859	missense	probably benign	0.00
R8077:Olfr420	UTSW	1	174151845	unclassified	probably benign
R8519:Olfr420	UTSW	1	174159048	missense	probably damaging	0.99
R9106:Olfr420	UTSW	1	174158803	missense	probably benign	0.00
R9205:Olfr420	UTSW	1	174158890	missense	probably benign	0.33
R9507:Olfr420	UTSW	1	174158986	missense	possibly damaging	0.94
Z1187:Olfr420	UTSW	1	174159341	nonsense	probably null
Z1192:Olfr420	UTSW	1	174159341	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CACCATTGTGGAGTACATATGATG -3'
(R):5'- TGGTGTACCATATCTCCAGGAAG -3'

Sequencing Primer
(F):5'- TCAAAGCCTGGCTTTTGG -3'
(R):5'- CACTGATGAAGAAGTACATGGGTGTG -3'

Posted On

2020-01-17