Mutagenetix > Incidental Mutation

Incidental Mutation 'R7948:Lama5'

615961

Institutional Source

Beutler Lab

Gene Symbol

Lama5

Ensembl Gene

ENSMUSG00000015647

Gene Name

laminin, alpha 5

Synonyms

MMRRC Submission

045993-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7948 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

180176373-180225859 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 180202201 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 389 (D389G)

Ref Sequence

ENSEMBL: ENSMUSP00000015791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015791]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000015791
AA Change: D389G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647
AA Change: D389G

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

Strain: 3624772; 1934917
Lethality: E1-E17
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit disrupted basal laminae leading to exencephaly, syndactyly, placentopathy, kidney defects, abnormal lobar septation with absence of a visceral pleural membrane, and lethality in late gestation. [provided by MGI curators]

Allele List at MGI

All alleles(49) : Targeted(5) Gene trapped(44)

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	CGGTCTAGCTGAGCAGGAGGCAGCTCAGG	CGG	14: 70,157,366 (GRCm38)		probably null	Het
Abca8a	A	T	11: 110,050,979 (GRCm38)	Y1155N	probably benign	Het
Adcy8	C	T	15: 64,815,350 (GRCm38)	R435K	possibly damaging	Het
Adgrv1	T	C	13: 81,559,588 (GRCm38)	D1233G	probably damaging	Het
Adgrv1	C	A	13: 81,559,529 (GRCm38)	V1253F	probably damaging	Het
Baz2a	G	A	10: 128,125,325 (GRCm38)	R1639H	possibly damaging	Het
Ccdc125	A	G	13: 100,696,402 (GRCm38)	T496A	probably benign	Het
Cntnap5a	A	G	1: 116,580,528 (GRCm38)	M1257V	probably benign	Het
Cpne3	A	G	4: 19,528,186 (GRCm38)		probably null	Het
Cts3	T	C	13: 61,566,049 (GRCm38)	E288G	probably benign	Het
Deup1	T	C	9: 15,610,648 (GRCm38)	K74E	possibly damaging	Het
Epn1	T	A	7: 5,089,993 (GRCm38)	Y101*	probably null	Het
Ercc4	A	G	16: 13,130,185 (GRCm38)	D422G	probably benign	Het
Exoc6	A	C	19: 37,576,974 (GRCm38)	N166T	probably benign	Het
Fam89a	T	C	8: 124,751,670 (GRCm38)	Y47C	probably damaging	Het
Fbn1	C	A	2: 125,341,299 (GRCm38)	Q1753H	probably damaging	Het
Gal3st4	C	T	5: 138,271,000 (GRCm38)	R66Q	probably benign	Het
Gatad1	T	C	5: 3,643,540 (GRCm38)	R210G	probably benign	Het
Golm1	C	A	13: 59,664,197 (GRCm38)		probably null	Het
Gtpbp3	A	G	8: 71,492,586 (GRCm38)	H434R	probably damaging	Het
Hbegf	A	G	18: 36,506,699 (GRCm38)	L194S	possibly damaging	Het
Igsf10	A	G	3: 59,331,858 (GRCm38)	S301P	probably benign	Het
Il9r	A	C	11: 32,194,486 (GRCm38)	C106W	probably damaging	Het
Lyst	T	A	13: 13,746,589 (GRCm38)	D3373E	possibly damaging	Het
Mkrn1	T	A	6: 39,400,410 (GRCm38)	Y361F	probably benign	Het
Mtrex	C	T	13: 112,921,762 (GRCm38)	R45Q	probably benign	Het
Muc16	A	T	9: 18,642,490 (GRCm38)	I4169N	unknown	Het
Myo7a	C	T	7: 98,075,029 (GRCm38)	G1150S	probably damaging	Het
Myom2	A	G	8: 15,085,306 (GRCm38)	D503G	probably benign	Het
Nmnat3	A	G	9: 98,399,482 (GRCm38)	I46V	probably benign	Het
Nrp2	C	T	1: 62,745,408 (GRCm38)	R239C	probably damaging	Het
Nrros	A	T	16: 32,162,258 (GRCm38)	N17K	unknown	Het
Or51a39	C	T	7: 102,713,688 (GRCm38)	V242I	probably benign	Het
Patj	A	T	4: 98,424,310 (GRCm38)	K295M	probably damaging	Het
Pax6	A	G	2: 105,685,877 (GRCm38)	T167A	probably benign	Het
Pclo	T	A	5: 14,765,166 (GRCm38)	L1212*	probably null	Het
Peg3	T	A	7: 6,708,782 (GRCm38)	Y1147F	probably damaging	Het
Ppp2r5c	T	A	12: 110,465,986 (GRCm38)	N77K	probably benign	Het
Prickle2	T	C	6: 92,416,922 (GRCm38)	I257V	possibly damaging	Het
Ptprc	G	A	1: 138,064,576 (GRCm38)	T1132I	probably benign	Het
Serpinb6a	G	A	13: 33,923,020 (GRCm38)	S183L	probably benign	Het
Slc6a15	T	A	10: 103,404,295 (GRCm38)	M293K	possibly damaging	Het
Tmem131	A	T	1: 36,794,148 (GRCm38)	W1749R	probably damaging	Het
Trpm8	T	A	1: 88,374,369 (GRCm38)	Y1020*	probably null	Het
Ttn	G	T	2: 76,768,179 (GRCm38)	Y19463*	probably null	Het
Tubgcp5	T	A	7: 55,794,248 (GRCm38)	D18E	probably benign	Het
Ubtd1	T	A	19: 42,033,735 (GRCm38)	F149I	probably benign	Het
Xirp2	A	G	2: 67,519,314 (GRCm38)	K3279R	possibly damaging	Het
Zfp236	T	C	18: 82,624,415 (GRCm38)	T1117A	probably damaging	Het
Zfp945	A	T	17: 22,852,122 (GRCm38)	C289S	unknown	Het

Other mutations in Lama5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Lama5	APN	2	180,176,543 (GRCm38)	unclassified	probably benign
IGL01370:Lama5	APN	2	180,197,400 (GRCm38)	missense	possibly damaging	0.87
IGL01474:Lama5	APN	2	180,196,570 (GRCm38)	missense	probably damaging	1.00
IGL01614:Lama5	APN	2	180,180,864 (GRCm38)	missense	probably damaging	1.00
IGL01941:Lama5	APN	2	180,192,392 (GRCm38)	missense	possibly damaging	0.71
IGL01953:Lama5	APN	2	180,190,704 (GRCm38)	missense	probably damaging	0.97
IGL02093:Lama5	APN	2	180,188,587 (GRCm38)	missense	probably damaging	1.00
IGL02197:Lama5	APN	2	180,207,219 (GRCm38)	missense	possibly damaging	0.82
IGL02308:Lama5	APN	2	180,190,327 (GRCm38)	splice site	probably benign
IGL02314:Lama5	APN	2	180,194,482 (GRCm38)	splice site	probably benign
IGL02317:Lama5	APN	2	180,191,319 (GRCm38)	missense	probably damaging	1.00
IGL02354:Lama5	APN	2	180,193,884 (GRCm38)	nonsense	probably null
IGL02361:Lama5	APN	2	180,193,884 (GRCm38)	nonsense	probably null
IGL02557:Lama5	APN	2	180,190,932 (GRCm38)	nonsense	probably null
IGL03026:Lama5	APN	2	180,195,967 (GRCm38)	missense	probably benign	0.34
IGL03160:Lama5	APN	2	180,180,335 (GRCm38)	missense	probably damaging	1.00
IGL03238:Lama5	APN	2	180,188,574 (GRCm38)	missense	probably benign
IGL03390:Lama5	APN	2	180,207,218 (GRCm38)	missense	probably damaging	1.00
blancmange	UTSW	2	180,180,611 (GRCm38)	missense	probably damaging	0.98
cupcake	UTSW	2	180,185,959 (GRCm38)	missense	probably damaging	1.00
layercake	UTSW	2	180,180,718 (GRCm38)	missense	possibly damaging	0.83
poundcake	UTSW	2	180,195,608 (GRCm38)	missense	probably damaging	1.00
Salty	UTSW	2	180,181,651 (GRCm38)	missense	possibly damaging	0.84
PIT4378001:Lama5	UTSW	2	180,189,445 (GRCm38)	missense	possibly damaging	0.89
R0003:Lama5	UTSW	2	180,178,079 (GRCm38)	splice site	probably null
R0056:Lama5	UTSW	2	180,187,106 (GRCm38)	intron	probably benign
R0147:Lama5	UTSW	2	180,190,406 (GRCm38)	missense	probably benign
R0148:Lama5	UTSW	2	180,190,406 (GRCm38)	missense	probably benign
R0310:Lama5	UTSW	2	180,181,566 (GRCm38)	splice site	probably benign
R0326:Lama5	UTSW	2	180,182,426 (GRCm38)	missense	possibly damaging	0.90
R0368:Lama5	UTSW	2	180,181,230 (GRCm38)	nonsense	probably null
R0479:Lama5	UTSW	2	180,184,457 (GRCm38)	missense	probably benign	0.03
R0490:Lama5	UTSW	2	180,180,169 (GRCm38)	missense	possibly damaging	0.90
R0636:Lama5	UTSW	2	180,189,331 (GRCm38)	critical splice donor site	probably null
R0704:Lama5	UTSW	2	180,179,484 (GRCm38)	missense	possibly damaging	0.84
R0733:Lama5	UTSW	2	180,180,718 (GRCm38)	missense	possibly damaging	0.83
R1017:Lama5	UTSW	2	180,195,420 (GRCm38)	missense	probably damaging	1.00
R1078:Lama5	UTSW	2	180,179,764 (GRCm38)	unclassified	probably benign
R1294:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1423:Lama5	UTSW	2	180,195,641 (GRCm38)	missense	probably damaging	1.00
R1438:Lama5	UTSW	2	180,182,800 (GRCm38)	missense	probably benign	0.01
R1447:Lama5	UTSW	2	180,185,878 (GRCm38)	missense	probably damaging	0.99
R1540:Lama5	UTSW	2	180,180,151 (GRCm38)	missense	probably benign
R1601:Lama5	UTSW	2	180,197,745 (GRCm38)	missense	probably damaging	1.00
R1624:Lama5	UTSW	2	180,206,758 (GRCm38)	missense	probably benign	0.02
R1674:Lama5	UTSW	2	180,201,987 (GRCm38)	missense	probably benign	0.00
R1687:Lama5	UTSW	2	180,194,066 (GRCm38)	missense	probably benign	0.00
R1696:Lama5	UTSW	2	180,202,486 (GRCm38)	missense	probably damaging	1.00
R1701:Lama5	UTSW	2	180,221,369 (GRCm38)	missense	probably damaging	1.00
R1778:Lama5	UTSW	2	180,195,481 (GRCm38)	splice site	probably benign
R1936:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1939:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1940:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R1953:Lama5	UTSW	2	180,190,747 (GRCm38)	missense	possibly damaging	0.94
R1966:Lama5	UTSW	2	180,188,352 (GRCm38)	missense	probably damaging	1.00
R2024:Lama5	UTSW	2	180,179,130 (GRCm38)	missense	probably benign	0.00
R2079:Lama5	UTSW	2	180,225,508 (GRCm38)	missense	possibly damaging	0.68
R2115:Lama5	UTSW	2	180,186,885 (GRCm38)	missense	probably damaging	1.00
R2173:Lama5	UTSW	2	180,196,242 (GRCm38)	missense	probably benign	0.00
R2272:Lama5	UTSW	2	180,178,603 (GRCm38)	missense	possibly damaging	0.93
R2357:Lama5	UTSW	2	180,180,097 (GRCm38)	missense	probably benign	0.01
R2860:Lama5	UTSW	2	180,187,247 (GRCm38)	missense	probably benign	0.00
R2861:Lama5	UTSW	2	180,187,247 (GRCm38)	missense	probably benign	0.00
R2939:Lama5	UTSW	2	180,198,954 (GRCm38)	missense	probably damaging	1.00
R3053:Lama5	UTSW	2	180,183,067 (GRCm38)	missense	probably damaging	0.99
R3430:Lama5	UTSW	2	180,196,317 (GRCm38)	missense	probably benign	0.00
R3752:Lama5	UTSW	2	180,187,222 (GRCm38)	missense	probably damaging	1.00
R3782:Lama5	UTSW	2	180,194,563 (GRCm38)	missense	possibly damaging	0.57
R3901:Lama5	UTSW	2	180,182,351 (GRCm38)	splice site	probably benign
R4248:Lama5	UTSW	2	180,180,427 (GRCm38)	missense	possibly damaging	0.84
R4626:Lama5	UTSW	2	180,184,460 (GRCm38)	missense	probably damaging	0.98
R4638:Lama5	UTSW	2	180,190,413 (GRCm38)	missense	possibly damaging	0.89
R4669:Lama5	UTSW	2	180,180,637 (GRCm38)	missense	probably damaging	1.00
R4673:Lama5	UTSW	2	180,199,266 (GRCm38)	missense	probably damaging	1.00
R4677:Lama5	UTSW	2	180,179,366 (GRCm38)	missense	possibly damaging	0.69
R4701:Lama5	UTSW	2	180,191,696 (GRCm38)	missense	probably damaging	1.00
R4774:Lama5	UTSW	2	180,185,941 (GRCm38)	missense	probably damaging	1.00
R4880:Lama5	UTSW	2	180,177,068 (GRCm38)	unclassified	probably benign
R4923:Lama5	UTSW	2	180,184,149 (GRCm38)	missense	probably benign	0.18
R4960:Lama5	UTSW	2	180,208,252 (GRCm38)	critical splice donor site	probably null
R4983:Lama5	UTSW	2	180,193,449 (GRCm38)	missense	probably benign	0.13
R5061:Lama5	UTSW	2	180,198,786 (GRCm38)	nonsense	probably null
R5080:Lama5	UTSW	2	180,207,200 (GRCm38)	nonsense	probably null
R5135:Lama5	UTSW	2	180,202,220 (GRCm38)	missense	possibly damaging	0.89
R5206:Lama5	UTSW	2	180,191,304 (GRCm38)	missense	probably damaging	1.00
R5296:Lama5	UTSW	2	180,193,801 (GRCm38)	missense	probably damaging	1.00
R5319:Lama5	UTSW	2	180,181,118 (GRCm38)	missense	probably damaging	1.00
R5355:Lama5	UTSW	2	180,181,651 (GRCm38)	missense	possibly damaging	0.84
R5388:Lama5	UTSW	2	180,190,746 (GRCm38)	missense	possibly damaging	0.83
R5528:Lama5	UTSW	2	180,194,563 (GRCm38)	missense	probably benign	0.21
R5536:Lama5	UTSW	2	180,189,349 (GRCm38)	missense	probably damaging	0.99
R5658:Lama5	UTSW	2	180,208,276 (GRCm38)	nonsense	probably null
R5823:Lama5	UTSW	2	180,192,492 (GRCm38)	missense	probably benign	0.04
R5885:Lama5	UTSW	2	180,201,831 (GRCm38)	missense	probably damaging	1.00
R5889:Lama5	UTSW	2	180,193,674 (GRCm38)	intron	probably benign
R5912:Lama5	UTSW	2	180,195,475 (GRCm38)	missense	probably damaging	1.00
R5955:Lama5	UTSW	2	180,197,474 (GRCm38)	missense	probably damaging	1.00
R6015:Lama5	UTSW	2	180,185,392 (GRCm38)	missense	probably benign	0.36
R6037:Lama5	UTSW	2	180,207,013 (GRCm38)	missense	probably damaging	1.00
R6037:Lama5	UTSW	2	180,207,013 (GRCm38)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	180,185,959 (GRCm38)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	180,180,611 (GRCm38)	missense	probably damaging	0.98
R6359:Lama5	UTSW	2	180,195,982 (GRCm38)	missense	probably benign	0.01
R6385:Lama5	UTSW	2	180,196,533 (GRCm38)	missense	probably damaging	1.00
R6406:Lama5	UTSW	2	180,197,464 (GRCm38)	nonsense	probably null
R6552:Lama5	UTSW	2	180,181,154 (GRCm38)	missense	probably damaging	0.98
R6632:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6633:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6645:Lama5	UTSW	2	180,179,670 (GRCm38)	missense	probably damaging	1.00
R6731:Lama5	UTSW	2	180,188,574 (GRCm38)	missense	probably benign	0.09
R6744:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6798:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6799:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6801:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6851:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6869:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6881:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6882:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R6884:Lama5	UTSW	2	180,191,662 (GRCm38)	missense	probably damaging	1.00
R7022:Lama5	UTSW	2	180,180,731 (GRCm38)	missense	probably damaging	1.00
R7204:Lama5	UTSW	2	180,202,177 (GRCm38)	missense	probably damaging	1.00
R7207:Lama5	UTSW	2	180,207,084 (GRCm38)	missense	probably damaging	0.98
R7282:Lama5	UTSW	2	180,201,795 (GRCm38)	missense	probably damaging	1.00
R7367:Lama5	UTSW	2	180,192,958 (GRCm38)	missense	probably benign	0.01
R7410:Lama5	UTSW	2	180,202,390 (GRCm38)	critical splice donor site	probably null
R7699:Lama5	UTSW	2	180,180,861 (GRCm38)	missense	probably damaging	1.00
R7849:Lama5	UTSW	2	180,201,812 (GRCm38)	missense	probably damaging	1.00
R7909:Lama5	UTSW	2	180,192,276 (GRCm38)	missense	possibly damaging	0.95
R8153:Lama5	UTSW	2	180,187,931 (GRCm38)	missense	probably benign	0.37
R8317:Lama5	UTSW	2	180,206,991 (GRCm38)	missense	probably damaging	1.00
R8351:Lama5	UTSW	2	180,195,608 (GRCm38)	missense	probably damaging	1.00
R8370:Lama5	UTSW	2	180,201,487 (GRCm38)	missense	possibly damaging	0.80
R8398:Lama5	UTSW	2	180,197,034 (GRCm38)	critical splice donor site	probably null
R8401:Lama5	UTSW	2	180,198,787 (GRCm38)	missense	probably damaging	1.00
R8404:Lama5	UTSW	2	180,195,222 (GRCm38)	missense	probably damaging	1.00
R8502:Lama5	UTSW	2	180,195,222 (GRCm38)	missense	probably damaging	1.00
R8694:Lama5	UTSW	2	180,180,884 (GRCm38)	missense	probably damaging	0.98
R8705:Lama5	UTSW	2	180,178,561 (GRCm38)	missense	probably damaging	1.00
R8732:Lama5	UTSW	2	180,186,688 (GRCm38)	missense	probably damaging	1.00
R8755:Lama5	UTSW	2	180,190,921 (GRCm38)	missense	probably benign	0.00
R8786:Lama5	UTSW	2	180,196,307 (GRCm38)	missense	probably damaging	1.00
R8926:Lama5	UTSW	2	180,193,990 (GRCm38)	missense	probably benign	0.08
R8928:Lama5	UTSW	2	180,202,039 (GRCm38)	missense	probably damaging	1.00
R8953:Lama5	UTSW	2	180,193,520 (GRCm38)	missense	probably damaging	0.99
R8958:Lama5	UTSW	2	180,193,799 (GRCm38)	missense	probably benign
R9002:Lama5	UTSW	2	180,196,518 (GRCm38)	missense	probably damaging	1.00
R9081:Lama5	UTSW	2	180,192,137 (GRCm38)	nonsense	probably null
R9165:Lama5	UTSW	2	180,179,493 (GRCm38)	missense	probably damaging	0.99
R9233:Lama5	UTSW	2	180,198,709 (GRCm38)	nonsense	probably null
R9264:Lama5	UTSW	2	180,196,478 (GRCm38)	splice site	probably benign
R9311:Lama5	UTSW	2	180,196,482 (GRCm38)	critical splice donor site	probably null
R9443:Lama5	UTSW	2	180,201,729 (GRCm38)	missense	probably benign	0.00
R9488:Lama5	UTSW	2	180,181,441 (GRCm38)	missense	possibly damaging	0.95
R9674:Lama5	UTSW	2	180,198,474 (GRCm38)	critical splice donor site	probably null
R9684:Lama5	UTSW	2	180,207,245 (GRCm38)	missense	probably damaging	1.00
R9749:Lama5	UTSW	2	180,183,640 (GRCm38)	missense	probably benign	0.00
RF020:Lama5	UTSW	2	180,196,178 (GRCm38)	missense	probably benign
X0065:Lama5	UTSW	2	180,181,731 (GRCm38)	missense	probably benign	0.26
Z1177:Lama5	UTSW	2	180,190,714 (GRCm38)	missense	possibly damaging	0.95
Z1177:Lama5	UTSW	2	180,189,419 (GRCm38)	missense	probably damaging	1.00
Z1177:Lama5	UTSW	2	180,183,630 (GRCm38)	missense	probably benign	0.03
Z1177:Lama5	UTSW	2	180,198,810 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACTTACGCCGACAGACATGAG -3'
(R):5'- TAGTATTCACTGCCTGCACC -3'

Sequencing Primer
(F):5'- CCGACAGACATGAGGTGAGTC -3'
(R):5'- CCATGGCTCACGGAATATGTC -3'

Posted On

2020-01-23