Mutagenetix > Incidental Mutation

Incidental Mutation 'R7948:Zfp945'

615998

Institutional Source

Beutler Lab

Gene Symbol

Zfp945

Ensembl Gene

ENSMUSG00000059142

Gene Name

zinc finger protein 945

Synonyms

C730040L01Rik, A630033E08Rik

MMRRC Submission

045993-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R7948 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23065671-23086108 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23071096 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 289 (C289S)

Ref Sequence

ENSEMBL: ENSMUSP00000124123 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088696] [ENSMUST00000150092] [ENSMUST00000160457]

AlphaFold

F6WAU7

Predicted Effect

unknown
Transcript: ENSMUST00000088696
AA Change: C268S

SMART Domains

Protein: ENSMUSP00000086074
Gene: ENSMUSG00000059142
AA Change: C268S

Domain	Start	End	E-Value	Type
KRAB	13	68	9.12e-18	SMART
ZnF_C2H2	210	232	1.45e-2	SMART
ZnF_C2H2	238	260	1.03e-2	SMART
ZnF_C2H2	266	288	1.76e-1	SMART
ZnF_C2H2	322	344	7.37e-4	SMART
ZnF_C2H2	350	372	1.5e-4	SMART
ZnF_C2H2	378	400	5.14e-3	SMART
ZnF_C2H2	406	428	9.73e-4	SMART
ZnF_C2H2	434	456	2.99e-4	SMART
ZnF_C2H2	462	484	4.54e-4	SMART
ZnF_C2H2	490	512	2.4e-3	SMART
ZnF_C2H2	518	540	1.38e-3	SMART
ZnF_C2H2	546	568	1.13e-4	SMART
ZnF_C2H2	574	596	5.59e-4	SMART
ZnF_C2H2	602	624	9.31e1	SMART
ZnF_C2H2	630	652	7.9e-4	SMART
ZnF_C2H2	658	680	3.69e-4	SMART
ZnF_C2H2	686	708	3.16e-3	SMART
ZnF_C2H2	714	736	2.95e-3	SMART
ZnF_C2H2	742	764	1.98e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000150092

SMART Domains

Protein: ENSMUSP00000117978
Gene: ENSMUSG00000059142

Domain	Start	End	E-Value	Type
KRAB	34	89	9.12e-18	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000160457
AA Change: C289S

SMART Domains

Protein: ENSMUSP00000124123
Gene: ENSMUSG00000059142
AA Change: C289S

Domain	Start	End	E-Value	Type
KRAB	34	89	9.12e-18	SMART
ZnF_C2H2	231	253	1.45e-2	SMART
ZnF_C2H2	259	281	1.03e-2	SMART
ZnF_C2H2	287	309	1.76e-1	SMART
ZnF_C2H2	343	365	7.37e-4	SMART
ZnF_C2H2	371	393	1.5e-4	SMART
ZnF_C2H2	399	421	5.14e-3	SMART
ZnF_C2H2	427	449	9.73e-4	SMART
ZnF_C2H2	455	477	2.99e-4	SMART
ZnF_C2H2	483	505	4.54e-4	SMART
ZnF_C2H2	511	533	2.4e-3	SMART
ZnF_C2H2	539	561	1.38e-3	SMART
ZnF_C2H2	567	589	1.13e-4	SMART
ZnF_C2H2	595	617	5.59e-4	SMART
ZnF_C2H2	623	645	9.31e1	SMART
ZnF_C2H2	651	673	7.9e-4	SMART
ZnF_C2H2	679	701	3.69e-4	SMART
ZnF_C2H2	707	729	3.16e-3	SMART
ZnF_C2H2	735	757	2.95e-3	SMART
ZnF_C2H2	763	785	1.98e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	CGGTCTAGCTGAGCAGGAGGCAGCTCAGG	CGG	14: 70,394,815 (GRCm39)		probably null	Het
Abca8a	A	T	11: 109,941,805 (GRCm39)	Y1155N	probably benign	Het
Adcy8	C	T	15: 64,687,199 (GRCm39)	R435K	possibly damaging	Het
Adgrv1	C	A	13: 81,707,648 (GRCm39)	V1253F	probably damaging	Het
Adgrv1	T	C	13: 81,707,707 (GRCm39)	D1233G	probably damaging	Het
Baz2a	G	A	10: 127,961,194 (GRCm39)	R1639H	possibly damaging	Het
Ccdc125	A	G	13: 100,832,910 (GRCm39)	T496A	probably benign	Het
Cntnap5a	A	G	1: 116,508,258 (GRCm39)	M1257V	probably benign	Het
Cpne3	A	G	4: 19,528,186 (GRCm39)		probably null	Het
Cts3	T	C	13: 61,713,863 (GRCm39)	E288G	probably benign	Het
Deup1	T	C	9: 15,521,944 (GRCm39)	K74E	possibly damaging	Het
Epn1	T	A	7: 5,092,992 (GRCm39)	Y101*	probably null	Het
Ercc4	A	G	16: 12,948,049 (GRCm39)	D422G	probably benign	Het
Exoc6	A	C	19: 37,565,422 (GRCm39)	N166T	probably benign	Het
Fam89a	T	C	8: 125,478,409 (GRCm39)	Y47C	probably damaging	Het
Fbn1	C	A	2: 125,183,219 (GRCm39)	Q1753H	probably damaging	Het
Gal3st4	C	T	5: 138,269,262 (GRCm39)	R66Q	probably benign	Het
Gatad1	T	C	5: 3,693,540 (GRCm39)	R210G	probably benign	Het
Golm1	C	A	13: 59,812,011 (GRCm39)		probably null	Het
Gtpbp3	A	G	8: 71,945,230 (GRCm39)	H434R	probably damaging	Het
Hbegf	A	G	18: 36,639,752 (GRCm39)	L194S	possibly damaging	Het
Igsf10	A	G	3: 59,239,279 (GRCm39)	S301P	probably benign	Het
Il9r	A	C	11: 32,144,486 (GRCm39)	C106W	probably damaging	Het
Lama5	T	C	2: 179,843,994 (GRCm39)	D389G	probably damaging	Het
Lyst	T	A	13: 13,921,174 (GRCm39)	D3373E	possibly damaging	Het
Mkrn1	T	A	6: 39,377,344 (GRCm39)	Y361F	probably benign	Het
Mtrex	C	T	13: 113,058,296 (GRCm39)	R45Q	probably benign	Het
Muc16	A	T	9: 18,553,786 (GRCm39)	I4169N	unknown	Het
Myo7a	C	T	7: 97,724,236 (GRCm39)	G1150S	probably damaging	Het
Myom2	A	G	8: 15,135,306 (GRCm39)	D503G	probably benign	Het
Nmnat3	A	G	9: 98,281,535 (GRCm39)	I46V	probably benign	Het
Nrp2	C	T	1: 62,784,567 (GRCm39)	R239C	probably damaging	Het
Nrros	A	T	16: 31,981,076 (GRCm39)	N17K	unknown	Het
Or51a39	C	T	7: 102,362,895 (GRCm39)	V242I	probably benign	Het
Patj	A	T	4: 98,312,547 (GRCm39)	K295M	probably damaging	Het
Pax6	A	G	2: 105,516,222 (GRCm39)	T167A	probably benign	Het
Pclo	T	A	5: 14,815,180 (GRCm39)	L1212*	probably null	Het
Peg3	T	A	7: 6,711,781 (GRCm39)	Y1147F	probably damaging	Het
Ppp2r5c	T	A	12: 110,432,420 (GRCm39)	N77K	probably benign	Het
Prickle2	T	C	6: 92,393,903 (GRCm39)	I257V	possibly damaging	Het
Ptprc	G	A	1: 137,992,314 (GRCm39)	T1132I	probably benign	Het
Serpinb6a	G	A	13: 34,107,003 (GRCm39)	S183L	probably benign	Het
Slc6a15	T	A	10: 103,240,156 (GRCm39)	M293K	possibly damaging	Het
Tmem131	A	T	1: 36,833,229 (GRCm39)	W1749R	probably damaging	Het
Trpm8	T	A	1: 88,302,091 (GRCm39)	Y1020*	probably null	Het
Ttn	G	T	2: 76,598,523 (GRCm39)	Y19463*	probably null	Het
Tubgcp5	T	A	7: 55,443,996 (GRCm39)	D18E	probably benign	Het
Ubtd1	T	A	19: 42,022,174 (GRCm39)	F149I	probably benign	Het
Xirp2	A	G	2: 67,349,658 (GRCm39)	K3279R	possibly damaging	Het
Zfp236	T	C	18: 82,642,540 (GRCm39)	T1117A	probably damaging	Het

Other mutations in Zfp945

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00754:Zfp945	APN	17	23,070,931 (GRCm39)	unclassified	probably benign
IGL01077:Zfp945	APN	17	23,071,359 (GRCm39)	missense	probably damaging	0.99
IGL02706:Zfp945	APN	17	23,076,256 (GRCm39)	missense	probably damaging	1.00
IGL03408:Zfp945	APN	17	23,071,511 (GRCm39)	nonsense	probably null
Small-scale	UTSW	17	23,069,955 (GRCm39)	missense	probably damaging	1.00
R0427:Zfp945	UTSW	17	23,084,226 (GRCm39)	missense	probably benign	0.10
R0718:Zfp945	UTSW	17	23,070,004 (GRCm39)	missense	probably damaging	1.00
R1305:Zfp945	UTSW	17	23,071,360 (GRCm39)	missense	probably damaging	0.97
R1801:Zfp945	UTSW	17	23,070,736 (GRCm39)	missense	probably damaging	0.97
R1837:Zfp945	UTSW	17	23,070,247 (GRCm39)	missense	probably damaging	1.00
R2001:Zfp945	UTSW	17	23,076,223 (GRCm39)	critical splice donor site	probably null
R4193:Zfp945	UTSW	17	23,070,144 (GRCm39)	unclassified	probably benign
R4247:Zfp945	UTSW	17	23,069,583 (GRCm39)	missense	probably damaging	0.98
R5026:Zfp945	UTSW	17	23,069,859 (GRCm39)	missense	probably damaging	1.00
R5918:Zfp945	UTSW	17	23,069,955 (GRCm39)	missense	probably damaging	1.00
R6076:Zfp945	UTSW	17	23,070,432 (GRCm39)	missense	probably damaging	1.00
R6664:Zfp945	UTSW	17	23,071,339 (GRCm39)	missense	probably damaging	0.99
R6997:Zfp945	UTSW	17	23,071,543 (GRCm39)	nonsense	probably null
R7040:Zfp945	UTSW	17	23,071,264 (GRCm39)	missense	probably damaging	0.98
R7479:Zfp945	UTSW	17	23,070,340 (GRCm39)	missense	possibly damaging	0.93
R7566:Zfp945	UTSW	17	23,070,727 (GRCm39)	missense	possibly damaging	0.71
R8383:Zfp945	UTSW	17	23,070,798 (GRCm39)	missense	probably benign	0.05
R9776:Zfp945	UTSW	17	23,070,582 (GRCm39)	missense	possibly damaging	0.93
R9790:Zfp945	UTSW	17	23,071,228 (GRCm39)	missense	probably benign	0.33
R9791:Zfp945	UTSW	17	23,071,228 (GRCm39)	missense	probably benign	0.33
X0063:Zfp945	UTSW	17	23,071,202 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- GGATTTGCCACATTCTTTACATCTG -3'
(R):5'- GTCTGGAAAATTCTTCACGATGACC -3'

Sequencing Primer
(F):5'- GGCATTCACGGTAGGAAA -3'
(R):5'- ACGATGACCTCAAGCCTGAGTG -3'

Posted On

2020-01-23