|Institutional Source||Beutler Lab|
|Gene Name||eukaryotic translation initiation factor 2-alpha kinase 2|
|Synonyms||dsRNA-activated kinase, eIF-2 alpha, eIF-2 alpha, IFN- type I-induced and dsRNA-activated kinase, IFN-induced and double-stranded RNA-activated kinase, Pkr, Prkr, Tik|
|Is this an essential gene?||Possibly essential (E-score: 0.659)|
|Stock #||R8003 (G1)|
|Chromosomal Location||78852564-78882573 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to A at 78876223 bp|
|Amino Acid Change||Alanine to Serine at position 66 (A66S)|
|Ref Sequence||ENSEMBL: ENSMUSP00000024884 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000024884]|
|Predicted Effect||probably damaging
AA Change: A66S
PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)
AA Change: A66S
|Coding Region Coverage||
|Validation Efficiency||100% (51/51)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for disruptions in this gene display altered susceptibility to viral infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Eif2ak2||
(F):5'- TGTCAACAAGTCTCCTGGTG -3'
(R):5'- TTAAAGTCTGTCAGTGAGCCC -3'
(F):5'- CCTGGTGTATTTCTCACTCAGAAG -3'
(R):5'- AGTGAGCCCGGGTTCGAATC -3'