Mutagenetix > Incidental Mutation

Incidental Mutation 'R0676:Mrgprb8'

61653

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb8

Ensembl Gene

ENSMUSG00000050870

Gene Name

MAS-related GPR, member B8

Synonyms

MrgB8

MMRRC Submission

038861-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0676 (G1)

Quality Score

136

Status

Validated

Chromosome

Chromosomal Location

48038274-48039396 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 48038412 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 28 (M28L)

Ref Sequence

ENSEMBL: ENSMUSP00000052230 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056676]

AlphaFold

Q7TN51

Predicted Effect

probably benign
Transcript: ENSMUST00000056676
AA Change: M28L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000052230
Gene: ENSMUSG00000050870
AA Change: M28L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	37	219	3.9e-7	PFAM
low complexity region	302	327	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.4%
20x: 95.2%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310003L06Rik	A	G	5: 88,112,516 (GRCm39)		probably benign	Het
Arhgef25	A	G	10: 127,019,879 (GRCm39)		probably null	Het
B3galnt2	T	C	13: 14,170,378 (GRCm39)	S243P	probably benign	Het
Col11a2	A	G	17: 34,276,249 (GRCm39)	N799D	probably damaging	Het
Cpb1	T	C	3: 20,320,697 (GRCm39)		probably null	Het
Crot	A	C	5: 9,043,622 (GRCm39)		probably benign	Het
Ctnna3	A	C	10: 64,245,040 (GRCm39)	H451P	probably benign	Het
Cts6	C	T	13: 61,345,298 (GRCm39)		probably benign	Het
Dock2	T	C	11: 34,586,063 (GRCm39)	T540A	probably damaging	Het
Dysf	C	A	6: 84,090,318 (GRCm39)	F956L	probably benign	Het
Gabrg3	A	T	7: 56,374,169 (GRCm39)	Y466N	probably damaging	Het
Gm10845	T	A	14: 80,100,644 (GRCm39)		noncoding transcript	Het
H2-M5	A	G	17: 37,300,034 (GRCm39)	F47L	possibly damaging	Het
H4c9	T	C	13: 22,225,276 (GRCm39)		probably null	Het
Il1rl1	CTTGTTGTTGTTGTTGTTG	CTTGTTGTTGTTGTTGTTGTTG	1: 40,481,734 (GRCm39)		probably benign	Het
Immt	A	G	6: 71,828,828 (GRCm39)	S128G	probably benign	Het
Klb	A	T	5: 65,536,398 (GRCm39)	D576V	probably damaging	Het
Lpin1	A	T	12: 16,590,980 (GRCm39)	N817K	possibly damaging	Het
Lrrk1	C	T	7: 65,944,729 (GRCm39)	R627H	probably damaging	Het
Luzp1	A	G	4: 136,269,996 (GRCm39)	K740E	probably damaging	Het
Mapk9	T	C	11: 49,773,983 (GRCm39)	*382Q	probably null	Het
Mn1	A	G	5: 111,568,900 (GRCm39)	S957G	possibly damaging	Het
Myo1a	A	G	10: 127,555,749 (GRCm39)	I913V	probably benign	Het
Nolc1	T	A	19: 46,068,528 (GRCm39)		probably benign	Het
Pde4dip	A	C	3: 97,624,413 (GRCm39)		probably benign	Het
Rbpj	C	T	5: 53,803,390 (GRCm39)		probably benign	Het
Ric1	T	C	19: 29,555,047 (GRCm39)	I387T	probably benign	Het
Ruvbl1	A	G	6: 88,450,182 (GRCm39)	R58G	probably damaging	Het
Scarb1	C	A	5: 125,374,278 (GRCm39)		probably benign	Het
Sh3tc1	A	T	5: 35,876,458 (GRCm39)		probably benign	Het
Slc22a23	G	A	13: 34,379,462 (GRCm39)	T435I	probably damaging	Het
Slc22a26	A	T	19: 7,773,509 (GRCm39)		probably benign	Het
Taf6l	T	C	19: 8,750,733 (GRCm39)	I114V	probably benign	Het
Tbc1d8b	A	G	X: 138,613,025 (GRCm39)	S284G	possibly damaging	Het
Tmem131l	C	T	3: 83,842,122 (GRCm39)		probably benign	Het
Vmn2r115	C	T	17: 23,565,238 (GRCm39)	S375F	probably benign	Het

Other mutations in Mrgprb8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02031:Mrgprb8	APN	7	48,039,087 (GRCm39)	missense	probably benign	0.01
IGL02191:Mrgprb8	APN	7	48,038,527 (GRCm39)	missense	probably damaging	1.00
IGL02449:Mrgprb8	APN	7	48,038,431 (GRCm39)	nonsense	probably null
IGL02724:Mrgprb8	APN	7	48,039,121 (GRCm39)	missense	possibly damaging	0.85
IGL02927:Mrgprb8	APN	7	48,038,373 (GRCm39)	nonsense	probably null
astroclast1	UTSW	7	48,038,892 (GRCm39)	missense	probably benign	0.05
A4554:Mrgprb8	UTSW	7	48,039,156 (GRCm39)	missense	probably damaging	1.00
R0890:Mrgprb8	UTSW	7	48,038,777 (GRCm39)	nonsense	probably null
R2094:Mrgprb8	UTSW	7	48,038,953 (GRCm39)	missense	probably benign	0.16
R2102:Mrgprb8	UTSW	7	48,038,634 (GRCm39)	missense	possibly damaging	0.56
R4839:Mrgprb8	UTSW	7	48,038,656 (GRCm39)	missense	probably benign	0.18
R5370:Mrgprb8	UTSW	7	48,038,568 (GRCm39)	missense	probably benign	0.00
R5471:Mrgprb8	UTSW	7	48,038,471 (GRCm39)	missense	probably damaging	1.00
R5548:Mrgprb8	UTSW	7	48,038,778 (GRCm39)	missense	probably benign	0.29
R6165:Mrgprb8	UTSW	7	48,038,565 (GRCm39)	missense	possibly damaging	0.78
R6199:Mrgprb8	UTSW	7	48,039,051 (GRCm39)	missense	probably benign	0.00
R6315:Mrgprb8	UTSW	7	48,038,983 (GRCm39)	missense	probably damaging	1.00
R6797:Mrgprb8	UTSW	7	48,038,892 (GRCm39)	missense	probably benign	0.05
R6924:Mrgprb8	UTSW	7	48,038,871 (GRCm39)	missense	possibly damaging	0.71
R8219:Mrgprb8	UTSW	7	48,038,649 (GRCm39)	missense	possibly damaging	0.90
R8489:Mrgprb8	UTSW	7	48,038,701 (GRCm39)	missense	possibly damaging	0.86
R8806:Mrgprb8	UTSW	7	48,038,976 (GRCm39)	missense	possibly damaging	0.95
R9146:Mrgprb8	UTSW	7	48,039,200 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCACACAACATGAGACACTTGGGG -3'
(R):5'- AGAAAACAACGCCATGTGAATGTCG -3'

Sequencing Primer
(F):5'- TGGACCATCAACTACATTCGTG -3'
(R):5'- ACTGCCTAATGAGATTTTCCAGGG -3'

Posted On

2013-07-30