Incidental Mutation 'R8004:Msh6'
ID 616543
Institutional Source Beutler Lab
Gene Symbol Msh6
Ensembl Gene ENSMUSG00000005370
Gene Name mutS homolog 6
Synonyms Gtmbp, GTBP, Msh6
MMRRC Submission 046044-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R8004 (G1)
Quality Score 225.009
Status Validated
Chromosome 17
Chromosomal Location 88282490-88298320 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 88294215 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Glycine at position 990 (E990G)
Ref Sequence ENSEMBL: ENSMUSP00000005503 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000005503] [ENSMUST00000005504]
AlphaFold P54276
Predicted Effect probably damaging
Transcript: ENSMUST00000005503
AA Change: E990G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000005503
Gene: ENSMUSG00000005370
AA Change: E990G

DomainStartEndE-ValueType
low complexity region 23 46 N/A INTRINSIC
low complexity region 76 87 N/A INTRINSIC
PWWP 90 152 9.01e-30 SMART
low complexity region 198 212 N/A INTRINSIC
low complexity region 216 230 N/A INTRINSIC
low complexity region 239 264 N/A INTRINSIC
low complexity region 273 291 N/A INTRINSIC
low complexity region 373 389 N/A INTRINSIC
Pfam:MutS_I 406 525 4.7e-35 PFAM
Pfam:MutS_II 536 700 1.4e-10 PFAM
MUTSd 750 1100 4.56e-86 SMART
MUTSac 1125 1319 1.68e-116 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000005504
SMART Domains Protein: ENSMUSP00000005504
Gene: ENSMUSG00000005371

DomainStartEndE-ValueType
low complexity region 2 20 N/A INTRINSIC
low complexity region 21 73 N/A INTRINSIC
FBOX 162 202 2.44e-8 SMART
PbH1 398 420 1.37e3 SMART
PbH1 421 443 8.83e0 SMART
CASH 421 557 1.31e-7 SMART
PbH1 444 466 6.15e1 SMART
PbH1 467 489 1.78e3 SMART
PbH1 490 512 2.29e2 SMART
PbH1 513 535 7.67e2 SMART
PbH1 536 558 1.36e0 SMART
PbH1 559 581 3.59e0 SMART
CASH 573 695 2.35e0 SMART
PbH1 582 604 8.73e2 SMART
PbH1 605 627 4.28e2 SMART
PbH1 628 650 5.03e2 SMART
PbH1 651 673 3.79e1 SMART
PbH1 674 696 4.73e0 SMART
PbH1 697 719 1.86e2 SMART
CASH 711 840 9.31e-13 SMART
PbH1 720 742 2.91e0 SMART
PbH1 743 765 3.73e2 SMART
PbH1 766 788 1.62e2 SMART
PbH1 789 811 9.99e1 SMART
PbH1 812 833 1.21e3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000130379
SMART Domains Protein: ENSMUSP00000121206
Gene: ENSMUSG00000005371

DomainStartEndE-ValueType
FBOX 87 127 2.44e-8 SMART
PbH1 323 345 1.37e3 SMART
PbH1 346 368 8.83e0 SMART
CASH 346 482 1.31e-7 SMART
PbH1 369 391 6.15e1 SMART
PbH1 392 414 1.78e3 SMART
PbH1 415 437 2.29e2 SMART
PbH1 438 460 7.67e2 SMART
PbH1 461 483 1.36e0 SMART
PbH1 484 506 3.59e0 SMART
CASH 498 620 2.35e0 SMART
PbH1 507 529 8.73e2 SMART
PbH1 530 552 4.28e2 SMART
PbH1 553 575 5.03e2 SMART
PbH1 576 598 3.79e1 SMART
PbH1 599 621 4.73e0 SMART
PbH1 622 644 1.86e2 SMART
CASH 636 765 9.31e-13 SMART
PbH1 645 667 2.91e0 SMART
PbH1 668 690 3.73e2 SMART
PbH1 691 713 1.62e2 SMART
PbH1 714 736 9.99e1 SMART
PbH1 737 758 1.21e3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000135639
Meta Mutation Damage Score 0.0633 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (61/61)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit premature death and are predisposed to tumor formation. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A830018L16Rik T A 1: 12,021,286 (GRCm39) probably benign Het
Abcb11 T A 2: 69,087,554 (GRCm39) K1070N possibly damaging Het
Ankrd24 T A 10: 81,474,191 (GRCm39) N179K unknown Het
Atp6v0a4 A T 6: 38,027,484 (GRCm39) F775L possibly damaging Het
Bbs2 A G 8: 94,809,118 (GRCm39) V269A possibly damaging Het
Bpifc T G 10: 85,815,148 (GRCm39) S273R probably benign Het
Cdan1 C T 2: 120,561,924 (GRCm39) R20Q unknown Het
Cfap251 T C 5: 123,392,513 (GRCm39) W216R unknown Het
Chrm2 T A 6: 36,500,221 (GRCm39) I26N probably damaging Het
Cngb3 A G 4: 19,505,273 (GRCm39) K669E possibly damaging Het
Cnot1 T C 8: 96,479,380 (GRCm39) I876V probably benign Het
Col12a1 T A 9: 79,591,683 (GRCm39) T926S probably damaging Het
Cyp2b23 G T 7: 26,378,891 (GRCm39) T191K probably benign Het
Dipk1a T C 5: 108,057,532 (GRCm39) D342G probably damaging Het
Dpp4 A G 2: 62,189,172 (GRCm39) V453A probably benign Het
Eif2ak4 T C 2: 118,247,775 (GRCm39) S271P possibly damaging Het
Fbxw20 G T 9: 109,050,449 (GRCm39) P392H probably damaging Het
Fcho2 T A 13: 98,926,013 (GRCm39) E118V possibly damaging Het
Gm2042 C G 12: 87,926,921 (GRCm39) Q413E possibly damaging Het
Gm904 G A 13: 50,799,343 (GRCm39) probably null Het
H2-D1 A G 17: 35,485,672 (GRCm39) S352G probably benign Het
Igf2bp3 C A 6: 49,067,954 (GRCm39) V451L probably benign Het
Igkv1-99 T C 6: 68,519,112 (GRCm39) V23A Het
Igsf10 A G 3: 59,237,130 (GRCm39) I1017T probably benign Het
Il18r1 G A 1: 40,513,917 (GRCm39) C41Y probably damaging Het
Itgb4 A G 11: 115,873,531 (GRCm39) D249G probably benign Het
Ldlrad1 G A 4: 107,066,688 (GRCm39) A8T probably benign Het
Lrrn2 T A 1: 132,865,489 (GRCm39) F185I probably damaging Het
Mllt6 G T 11: 97,566,966 (GRCm39) E635D possibly damaging Het
Myo1h A T 5: 114,458,769 (GRCm39) D184V Het
Nfatc2ip A G 7: 125,989,577 (GRCm39) V269A possibly damaging Het
Nfkb1 C A 3: 135,297,312 (GRCm39) probably benign Het
Nol12 A T 15: 78,824,717 (GRCm39) K181N probably damaging Het
Npl T A 1: 153,379,286 (GRCm39) T266S probably benign Het
Oog2 A T 4: 143,920,821 (GRCm39) I85L probably benign Het
Or5k3 T A 16: 58,969,351 (GRCm39) V46E probably damaging Het
Or7e171-ps1 A T 9: 19,853,408 (GRCm39) D109E possibly damaging Het
Parm1 A C 5: 91,742,132 (GRCm39) S167R probably benign Het
Pcsk4 T C 10: 80,158,674 (GRCm39) D526G probably damaging Het
Plscr4 C T 9: 92,372,843 (GRCm39) R322* probably null Het
Psmd9 T C 5: 123,379,998 (GRCm39) probably null Het
Ralgapa1 A G 12: 55,749,242 (GRCm39) V1220A probably damaging Het
Rbm25 A T 12: 83,721,166 (GRCm39) E577D possibly damaging Het
Rbm44 T A 1: 91,079,880 (GRCm39) probably benign Het
S100a6 T C 3: 90,521,119 (GRCm39) F16L probably benign Het
Sar1a C A 10: 61,520,945 (GRCm39) H53Q probably benign Het
Setd2 G A 9: 110,421,613 (GRCm39) A282T Het
Slc35f5 A T 1: 125,517,624 (GRCm39) H474L probably damaging Het
Slc4a3 T C 1: 75,525,711 (GRCm39) probably null Het
Smc1b G A 15: 84,981,815 (GRCm39) S735F probably damaging Het
Sp3 A T 2: 72,800,552 (GRCm39) I531K possibly damaging Het
St6galnac5 A T 3: 152,545,802 (GRCm39) M252K probably damaging Het
Tas2r120 T A 6: 132,634,390 (GRCm39) H157Q possibly damaging Het
Tbc1d10b T A 7: 126,798,183 (GRCm39) S653C probably damaging Het
Tex10 T C 4: 48,452,047 (GRCm39) E693G possibly damaging Het
Thada A G 17: 84,499,633 (GRCm39) S1855P probably benign Het
Tjp2 G A 19: 24,091,484 (GRCm39) P559L probably damaging Het
Tmem184b A G 15: 79,246,966 (GRCm39) S368P probably damaging Het
Tnc G A 4: 63,902,894 (GRCm39) S1461L probably benign Het
Tnpo2 T C 8: 85,771,328 (GRCm39) F177S probably benign Het
Vmn1r58 A T 7: 5,413,506 (GRCm39) Y241* probably null Het
Other mutations in Msh6
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01691:Msh6 APN 17 88,292,907 (GRCm39) missense probably benign
IGL01834:Msh6 APN 17 88,293,140 (GRCm39) missense probably damaging 1.00
IGL01904:Msh6 APN 17 88,292,160 (GRCm39) missense probably benign
IGL01957:Msh6 APN 17 88,292,519 (GRCm39) missense possibly damaging 0.73
IGL02117:Msh6 APN 17 88,298,234 (GRCm39) unclassified probably benign
IGL02234:Msh6 APN 17 88,294,229 (GRCm39) missense probably damaging 1.00
IGL02512:Msh6 APN 17 88,292,160 (GRCm39) missense probably benign
IGL02651:Msh6 APN 17 88,296,943 (GRCm39) missense probably damaging 1.00
IGL03381:Msh6 APN 17 88,292,537 (GRCm39) missense probably damaging 1.00
medea UTSW 17 88,287,651 (GRCm39) nonsense probably null
medusa UTSW 17 88,295,891 (GRCm39) unclassified probably benign
PIT4449001:Msh6 UTSW 17 88,293,616 (GRCm39) missense probably damaging 0.96
R0196:Msh6 UTSW 17 88,287,788 (GRCm39) missense possibly damaging 0.95
R0324:Msh6 UTSW 17 88,294,048 (GRCm39) nonsense probably null
R0492:Msh6 UTSW 17 88,282,679 (GRCm39) missense probably benign
R0711:Msh6 UTSW 17 88,294,112 (GRCm39) missense probably damaging 1.00
R1065:Msh6 UTSW 17 88,295,891 (GRCm39) unclassified probably benign
R1454:Msh6 UTSW 17 88,292,186 (GRCm39) missense probably benign 0.00
R1740:Msh6 UTSW 17 88,293,150 (GRCm39) missense possibly damaging 0.72
R1770:Msh6 UTSW 17 88,287,651 (GRCm39) nonsense probably null
R1771:Msh6 UTSW 17 88,291,950 (GRCm39) missense probably benign 0.17
R1919:Msh6 UTSW 17 88,292,553 (GRCm39) missense probably benign 0.01
R1926:Msh6 UTSW 17 88,293,653 (GRCm39) missense probably benign
R2026:Msh6 UTSW 17 88,297,771 (GRCm39) missense probably damaging 1.00
R2095:Msh6 UTSW 17 88,295,661 (GRCm39) missense possibly damaging 0.93
R2097:Msh6 UTSW 17 88,292,844 (GRCm39) missense probably benign 0.00
R2149:Msh6 UTSW 17 88,293,516 (GRCm39) missense probably damaging 1.00
R2156:Msh6 UTSW 17 88,293,568 (GRCm39) nonsense probably null
R2167:Msh6 UTSW 17 88,296,911 (GRCm39) missense probably damaging 1.00
R2382:Msh6 UTSW 17 88,292,159 (GRCm39) missense probably benign
R3005:Msh6 UTSW 17 88,295,713 (GRCm39) missense probably benign 0.34
R3160:Msh6 UTSW 17 88,292,909 (GRCm39) missense probably damaging 1.00
R3162:Msh6 UTSW 17 88,292,909 (GRCm39) missense probably damaging 1.00
R3162:Msh6 UTSW 17 88,292,909 (GRCm39) missense probably damaging 1.00
R3774:Msh6 UTSW 17 88,293,609 (GRCm39) missense probably damaging 1.00
R3775:Msh6 UTSW 17 88,293,609 (GRCm39) missense probably damaging 1.00
R4350:Msh6 UTSW 17 88,292,012 (GRCm39) missense probably damaging 1.00
R4424:Msh6 UTSW 17 88,298,217 (GRCm39) nonsense probably null
R4499:Msh6 UTSW 17 88,287,697 (GRCm39) missense probably damaging 1.00
R4667:Msh6 UTSW 17 88,292,234 (GRCm39) missense possibly damaging 0.89
R4668:Msh6 UTSW 17 88,292,234 (GRCm39) missense possibly damaging 0.89
R4669:Msh6 UTSW 17 88,292,234 (GRCm39) missense possibly damaging 0.89
R4849:Msh6 UTSW 17 88,290,947 (GRCm39) missense possibly damaging 0.94
R5137:Msh6 UTSW 17 88,287,716 (GRCm39) missense possibly damaging 0.83
R5472:Msh6 UTSW 17 88,291,989 (GRCm39) missense possibly damaging 0.81
R5594:Msh6 UTSW 17 88,293,497 (GRCm39) missense probably benign 0.00
R5607:Msh6 UTSW 17 88,294,329 (GRCm39) missense probably damaging 1.00
R5608:Msh6 UTSW 17 88,294,329 (GRCm39) missense probably damaging 1.00
R5660:Msh6 UTSW 17 88,292,147 (GRCm39) missense possibly damaging 0.94
R6243:Msh6 UTSW 17 88,290,999 (GRCm39) missense possibly damaging 0.69
R6279:Msh6 UTSW 17 88,287,677 (GRCm39) missense probably damaging 1.00
R6357:Msh6 UTSW 17 88,291,888 (GRCm39) nonsense probably null
R6399:Msh6 UTSW 17 88,294,319 (GRCm39) missense probably damaging 1.00
R6453:Msh6 UTSW 17 88,293,167 (GRCm39) missense probably damaging 1.00
R6646:Msh6 UTSW 17 88,293,870 (GRCm39) missense possibly damaging 0.80
R7404:Msh6 UTSW 17 88,282,548 (GRCm39)
R7837:Msh6 UTSW 17 88,292,094 (GRCm39) missense probably damaging 1.00
R8296:Msh6 UTSW 17 88,294,340 (GRCm39) missense probably damaging 1.00
R8326:Msh6 UTSW 17 88,294,340 (GRCm39) missense probably damaging 1.00
R8377:Msh6 UTSW 17 88,292,598 (GRCm39) missense probably damaging 1.00
R8715:Msh6 UTSW 17 88,293,195 (GRCm39) missense probably benign
R9752:Msh6 UTSW 17 88,293,963 (GRCm39) missense probably damaging 1.00
X0026:Msh6 UTSW 17 88,298,042 (GRCm39) missense probably benign 0.00
X0026:Msh6 UTSW 17 88,293,609 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGCCTTTGACCATGAGAAGGC -3'
(R):5'- ACTGCCAGTCCTTGTGGTTC -3'

Sequencing Primer
(F):5'- TCACTCCAAAAGCAGGGTTTG -3'
(R):5'- TCTTGTCGAAGTTACAGAACAAGCG -3'
Posted On 2020-01-23