Mutagenetix > Incidental Mutation

Incidental Mutation 'R8006:Vmn2r14'

616620

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

046046-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.469) question?

Stock #

R8006 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109362822-109372488 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109368324 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Methionine at position 223 (L223M)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably benign
Transcript: ENSMUST00000170341
AA Change: L223M

PolyPhen 2 Score 0.411 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: L223M

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam20	T	A	8: 41,248,944 (GRCm39)	H351Q	probably benign	Het
Ahnak	T	C	19: 8,989,447 (GRCm39)	V3577A	possibly damaging	Het
Akap13	T	C	7: 75,229,444 (GRCm39)	S126P	probably damaging	Het
Akip1	A	G	7: 109,303,199 (GRCm39)	D14G	probably damaging	Het
Ankhd1	A	G	18: 36,781,772 (GRCm39)	R287G		Het
Ankrd13a	A	C	5: 114,942,484 (GRCm39)	*589S	probably null	Het
Ankrd34c	T	C	9: 89,611,889 (GRCm39)	I151V	probably damaging	Het
Ano5	T	A	7: 51,243,518 (GRCm39)	D880E	probably benign	Het
Arl3	A	C	19: 46,546,813 (GRCm39)	L4R	probably damaging	Het
Ate1	T	A	7: 130,069,118 (GRCm39)	Q340L	probably damaging	Het
Ccdc38	A	T	10: 93,391,448 (GRCm39)		probably null	Het
Celsr3	C	T	9: 108,706,306 (GRCm39)	P930S	probably damaging	Het
Cfhr4	A	G	1: 139,664,590 (GRCm39)	Y490H	probably damaging	Het
Cox18	C	T	5: 90,371,672 (GRCm39)	V43M	probably damaging	Het
Ctnna3	A	G	10: 63,417,790 (GRCm39)	K176R	probably benign	Het
Fam135b	T	C	15: 71,334,183 (GRCm39)	T1004A	probably benign	Het
Fam227a	A	G	15: 79,518,299 (GRCm39)	I335T	possibly damaging	Het
Gm14496	A	G	2: 181,637,669 (GRCm39)	I248V	probably benign	Het
Gm6871	T	C	7: 41,195,106 (GRCm39)	T544A	probably benign	Het
Grm6	A	T	11: 50,755,484 (GRCm39)	*872L	probably null	Het
Herc1	T	A	9: 66,352,842 (GRCm39)	Y2109*	probably null	Het
Hsd11b2	T	A	8: 106,245,735 (GRCm39)	V80E	possibly damaging	Het
Itgb3	T	C	11: 104,556,322 (GRCm39)	V721A	possibly damaging	Het
Jade1	A	T	3: 41,568,124 (GRCm39)	I731L	probably benign	Het
Khnyn	G	A	14: 56,125,047 (GRCm39)	V434I	probably benign	Het
Lrp1	A	G	10: 127,425,488 (GRCm39)	L714P	probably damaging	Het
Lrrc41	G	A	4: 115,952,085 (GRCm39)	E585K	possibly damaging	Het
Lrrfip1	A	G	1: 91,004,673 (GRCm39)	Y70C	probably damaging	Het
Mex3a	T	A	3: 88,444,393 (GRCm39)	C490S	probably damaging	Het
Mmp27	C	T	9: 7,578,985 (GRCm39)	R387C	probably damaging	Het
Mro	A	T	18: 74,010,577 (GRCm39)	D219V	possibly damaging	Het
Myot	T	A	18: 44,487,904 (GRCm39)	L407Q	probably damaging	Het
Nppa	T	A	4: 148,085,638 (GRCm39)	W82R	probably damaging	Het
Or10g6	T	G	9: 39,933,770 (GRCm39)	L27R	probably damaging	Het
Or52a20	T	A	7: 103,366,532 (GRCm39)	C244S	probably damaging	Het
Or5ak24	T	C	2: 85,260,318 (GRCm39)	N285S	probably damaging	Het
Phip	T	A	9: 82,772,179 (GRCm39)	I1123L	possibly damaging	Het
Ppip5k2	A	G	1: 97,661,831 (GRCm39)	I695T	probably benign	Het
Ppp1r10	A	T	17: 36,239,158 (GRCm39)	M347L	probably benign	Het
Reln	A	T	5: 22,104,082 (GRCm39)	C3296*	probably null	Het
Semp2l1	T	A	1: 32,586,005 (GRCm39)		probably benign	Het
Slf2	T	A	19: 44,930,756 (GRCm39)	L611Q	probably damaging	Het
Spire1	G	A	18: 67,634,251 (GRCm39)	Q396*	probably null	Het
Taf2	A	G	15: 54,912,097 (GRCm39)	F537L	probably damaging	Het
Tbc1d16	C	T	11: 119,046,898 (GRCm39)	E451K	probably damaging	Het
Tcof1	G	C	18: 60,962,123 (GRCm39)	A702G	possibly damaging	Het
Tmem267	T	A	13: 120,070,774 (GRCm39)	V143E	probably damaging	Het
Tyw1	C	T	5: 130,296,913 (GRCm39)	R177W	possibly damaging	Het
Vcp	A	T	4: 42,985,993 (GRCm39)	H340Q	probably benign	Het
Wnk4	A	G	11: 101,159,182 (GRCm39)	D533G	probably benign	Het
Wwp1	A	T	4: 19,650,174 (GRCm39)	C331S	probably benign	Het
Zik1	A	T	7: 10,224,100 (GRCm39)	C332*	probably null	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109,364,180 (GRCm39)	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109,369,285 (GRCm39)	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109,372,443 (GRCm39)	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109,368,275 (GRCm39)	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109,372,349 (GRCm39)	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109,367,933 (GRCm39)	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109,368,454 (GRCm39)	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109,367,882 (GRCm39)	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109,369,305 (GRCm39)	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109,369,292 (GRCm39)	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109,364,260 (GRCm39)	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109,363,973 (GRCm39)	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109,368,350 (GRCm39)	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109,367,702 (GRCm39)	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109,366,910 (GRCm39)	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109,366,762 (GRCm39)	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109,364,226 (GRCm39)	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109,372,440 (GRCm39)	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109,364,117 (GRCm39)	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109,367,838 (GRCm39)	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109,366,913 (GRCm39)	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109,369,109 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109,366,698 (GRCm39)	splice site	probably null
R2417:Vmn2r14	UTSW	5	109,372,329 (GRCm39)	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109,363,776 (GRCm39)	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109,372,431 (GRCm39)	missense	probably null	0.00
R3729:Vmn2r14	UTSW	5	109,364,095 (GRCm39)	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109,368,033 (GRCm39)	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109,369,370 (GRCm39)	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109,363,976 (GRCm39)	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109,369,384 (GRCm39)	splice site	probably null
R4896:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109,363,961 (GRCm39)	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109,365,442 (GRCm39)	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109,369,154 (GRCm39)	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109,368,261 (GRCm39)	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109,367,816 (GRCm39)	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109,365,486 (GRCm39)	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109,363,724 (GRCm39)	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109,369,222 (GRCm39)	missense	probably benign
R5985:Vmn2r14	UTSW	5	109,368,082 (GRCm39)	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109,369,133 (GRCm39)	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109,364,096 (GRCm39)	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109,364,140 (GRCm39)	missense	probably benign	0.06
R6944:Vmn2r14	UTSW	5	109,363,925 (GRCm39)	missense	probably benign	0.22
R7608:Vmn2r14	UTSW	5	109,369,276 (GRCm39)	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109,368,086 (GRCm39)	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109,369,219 (GRCm39)	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R8007:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109,368,420 (GRCm39)	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109,369,342 (GRCm39)	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109,369,340 (GRCm39)	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109,365,533 (GRCm39)	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109,367,893 (GRCm39)	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109,367,902 (GRCm39)	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109,367,783 (GRCm39)	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109,364,087 (GRCm39)	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109,369,112 (GRCm39)	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109,369,288 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109,368,176 (GRCm39)	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109,368,428 (GRCm39)	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109,367,962 (GRCm39)	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109,364,041 (GRCm39)	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109,369,126 (GRCm39)	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109,367,741 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- CCCATTGTGAGGTTGTGATCCAG -3'
(R):5'- GACCATTTAATCCTAAACTGAGTGACC -3'

Sequencing Primer
(F):5'- CACCTTCTAAAGCTGACTTCTAGGG -3'
(R):5'- ACTGAGTGACCATGACCAGTTTC -3'

Posted On

2020-01-23