Mutagenetix > Incidental Mutation

Incidental Mutation 'R8010:Atrnl1'

616885

Institutional Source

Beutler Lab

Gene Symbol

Atrnl1

Ensembl Gene

ENSMUSG00000054843

Gene Name

attractin like 1

Synonyms

Alp

MMRRC Submission

046050-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.210) question?

Stock #

R8010 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

57611034-58133338 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 57682446 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 587 (V587A)

Ref Sequence

ENSEMBL: ENSMUSP00000076514 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077282]

AlphaFold

Q6A051

Predicted Effect

probably benign
Transcript: ENSMUST00000077282
AA Change: V587A

PolyPhen 2 Score 0.137 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000076514
Gene: ENSMUSG00000054843
AA Change: V587A

Domain	Start	End	E-Value	Type
low complexity region	25	32	N/A	INTRINSIC
EGF	61	90	5.71e-1	SMART
CUB	92	208	1.43e-11	SMART
EGF	209	244	1.95e1	SMART
Pfam:EGF_2	248	279	5.8e-7	PFAM
Pfam:Kelch_5	350	391	2.1e-9	PFAM
Pfam:Kelch_6	354	401	5.8e-8	PFAM
Pfam:Kelch_4	465	517	4.3e-7	PFAM
Pfam:Kelch_1	519	573	2.7e-6	PFAM
PSI	613	656	3.38e-1	SMART
PSI	665	708	2e-3	SMART
PSI	714	759	1.72e-2	SMART
CLECT	747	872	2.86e-20	SMART
PSI	888	938	6.26e-5	SMART
PSI	941	1011	1.73e-7	SMART
EGF_Lam	1013	1056	1.07e-5	SMART
low complexity region	1157	1173	N/A	INTRINSIC
transmembrane domain	1229	1251	N/A	INTRINSIC
low complexity region	1261	1272	N/A	INTRINSIC
low complexity region	1326	1339	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit normal coat coloring and normal brain morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	T	A	6: 128,580,340 (GRCm38)	H130L	probably benign	Het
Abca1	A	G	4: 53,127,600 (GRCm38)	S140P	probably benign	Het
Aoc1	T	C	6: 48,905,648 (GRCm38)	Y153H	probably benign	Het
Arhgdib	A	G	6: 136,926,722 (GRCm38)	I118T	probably damaging	Het
Atp1a3	T	A	7: 24,980,645 (GRCm38)	E865V	possibly damaging	Het
Ccdc107	G	T	4: 43,495,768 (GRCm38)	E199*	probably null	Het
Cd55	A	T	1: 130,459,616 (GRCm38)	D148E	probably benign	Het
Cdc42ep1	C	T	15: 78,847,799 (GRCm38)	T148I	possibly damaging	Het
Cep164	A	T	9: 45,823,671 (GRCm38)	D19E	unknown	Het
Chsy3	A	G	18: 59,410,154 (GRCm38)	Y788C	probably damaging	Het
Cttnbp2	G	C	6: 18,426,093 (GRCm38)	A762G	possibly damaging	Het
Cubn	A	G	2: 13,336,086 (GRCm38)		probably null	Het
Ddr1	T	A	17: 35,691,492 (GRCm38)	M175L	possibly damaging	Het
Dicer1	C	A	12: 104,692,132 (GRCm38)	K1850N	probably damaging	Het
Dnajc6	C	T	4: 101,618,414 (GRCm38)	R495C	probably benign	Het
Ecpas	T	A	4: 58,832,681 (GRCm38)	Q893L	unknown	Het
Fbh1	A	T	2: 11,767,632 (GRCm38)	N79K	probably benign	Het
Frmpd1	T	A	4: 45,284,272 (GRCm38)	V1031D	possibly damaging	Het
Gm7298	A	G	6: 121,735,583 (GRCm38)	E118G	probably benign	Het
Hnrnpll	T	C	17: 80,061,956 (GRCm38)	T13A	unknown	Het
Jcad	A	G	18: 4,674,581 (GRCm38)	D781G	probably benign	Het
Kitl	A	T	10: 100,051,903 (GRCm38)	T25S	probably benign	Het
Krt5	T	C	15: 101,712,356 (GRCm38)	D152G	probably damaging	Het
Krtap5-5	T	C	7: 142,229,911 (GRCm38)	M1V	probably null	Het
Ktn1	C	T	14: 47,705,773 (GRCm38)	T862I	possibly damaging	Het
Mapk4	A	T	18: 73,930,576 (GRCm38)	I525N	probably benign	Het
Megf6	T	C	4: 154,270,507 (GRCm38)	F1457S	probably benign	Het
Mrm3	A	T	11: 76,250,347 (GRCm38)	S394C	probably damaging	Het
Mtmr4	T	G	11: 87,598,864 (GRCm38)	V71G	probably damaging	Het
Nek8	T	C	11: 78,176,596 (GRCm38)	Y4C	probably damaging	Het
Or13g1	T	A	7: 86,307,052 (GRCm38)	E20D	probably benign	Het
Or5ac21	T	C	16: 59,303,504 (GRCm38)	M117T	probably damaging	Het
Or7g30	A	T	9: 19,441,692 (GRCm38)	I260L	probably benign	Het
Pla2r1	T	C	2: 60,514,960 (GRCm38)	T351A	probably benign	Het
Plcb4	C	T	2: 135,907,560 (GRCm38)	T49M	probably benign	Het
Psg20	T	C	7: 18,681,067 (GRCm38)	D301G	probably benign	Het
Ptpn13	C	T	5: 103,559,937 (GRCm38)	Q1455*	probably null	Het
Rbbp8	A	T	18: 11,722,233 (GRCm38)	N505I	possibly damaging	Het
Rgs18	A	G	1: 144,756,000 (GRCm38)	C125R	probably benign	Het
Rpap2	G	A	5: 107,603,605 (GRCm38)	C105Y	probably damaging	Het
Scn10a	C	T	9: 119,661,167 (GRCm38)	G570R	possibly damaging	Het
Sell	C	T	1: 164,065,512 (GRCm38)	T99I	possibly damaging	Het
Slc14a1	A	T	18: 78,116,489 (GRCm38)	M63K	probably benign	Het
Sp140l2	G	A	1: 85,246,950 (GRCm38)	S288L	possibly damaging	Het
Syne2	A	T	12: 75,930,738 (GRCm38)	D1319V	probably benign	Het
Tdp2	C	T	13: 24,836,027 (GRCm38)	T99I	probably damaging	Het
Tet3	A	T	6: 83,403,246 (GRCm38)	S647T	unknown	Het
Tpd52l1	T	G	10: 31,358,013 (GRCm38)	D48A	possibly damaging	Het
Ttll10	T	C	4: 156,047,161 (GRCm38)	D169G	probably damaging	Het
Wars2	C	A	3: 99,216,830 (GRCm38)	L336I	probably benign	Het
Wdfy4	C	A	14: 32,971,627 (GRCm38)	W2906L		Het
Xdh	A	T	17: 73,909,317 (GRCm38)	Y711*	probably null	Het
Xirp1	T	A	9: 120,017,824 (GRCm38)	R664S	probably benign	Het

Other mutations in Atrnl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Atrnl1	APN	19	57,691,817 (GRCm38)	missense	probably benign	0.02
IGL00707:Atrnl1	APN	19	57,673,265 (GRCm38)	missense	probably damaging	0.96
IGL00921:Atrnl1	APN	19	57,702,153 (GRCm38)	missense	probably damaging	1.00
IGL01410:Atrnl1	APN	19	58,131,104 (GRCm38)	missense	probably damaging	1.00
IGL01468:Atrnl1	APN	19	57,699,712 (GRCm38)	missense	probably benign	0.02
IGL01756:Atrnl1	APN	19	57,652,948 (GRCm38)	missense	probably benign
IGL01971:Atrnl1	APN	19	57,753,283 (GRCm38)	missense	probably damaging	1.00
IGL02019:Atrnl1	APN	19	57,691,763 (GRCm38)	splice site	probably benign
IGL02580:Atrnl1	APN	19	57,714,576 (GRCm38)	splice site	probably benign
IGL02649:Atrnl1	APN	19	57,650,441 (GRCm38)	splice site	probably benign
IGL02676:Atrnl1	APN	19	57,691,884 (GRCm38)	missense	probably damaging	1.00
IGL03276:Atrnl1	APN	19	57,652,927 (GRCm38)	missense	probably damaging	0.99
IGL03379:Atrnl1	APN	19	57,642,541 (GRCm38)	missense	probably benign	0.02
Magnetogorsk	UTSW	19	57,630,306 (GRCm38)	missense	probably damaging	1.00
polar	UTSW	19	57,652,950 (GRCm38)	missense	probably benign	0.00
PIT4812001:Atrnl1	UTSW	19	57,731,623 (GRCm38)	missense	probably benign	0.08
R0109:Atrnl1	UTSW	19	57,755,517 (GRCm38)	missense	possibly damaging	0.78
R0308:Atrnl1	UTSW	19	57,753,288 (GRCm38)	missense	probably benign	0.04
R0394:Atrnl1	UTSW	19	57,673,176 (GRCm38)	missense	probably benign	0.10
R0734:Atrnl1	UTSW	19	57,654,861 (GRCm38)	missense	probably damaging	1.00
R0811:Atrnl1	UTSW	19	57,673,141 (GRCm38)	missense	probably benign	0.07
R0812:Atrnl1	UTSW	19	57,673,141 (GRCm38)	missense	probably benign	0.07
R1183:Atrnl1	UTSW	19	57,650,293 (GRCm38)	missense	probably damaging	0.97
R1213:Atrnl1	UTSW	19	57,638,462 (GRCm38)	missense	probably benign	0.25
R1344:Atrnl1	UTSW	19	57,935,705 (GRCm38)	critical splice donor site	probably null
R1418:Atrnl1	UTSW	19	57,935,705 (GRCm38)	critical splice donor site	probably null
R1707:Atrnl1	UTSW	19	57,686,737 (GRCm38)	missense	probably benign	0.00
R1748:Atrnl1	UTSW	19	57,714,702 (GRCm38)	missense	probably damaging	0.99
R2051:Atrnl1	UTSW	19	57,691,849 (GRCm38)	missense	probably benign	0.01
R2113:Atrnl1	UTSW	19	57,755,616 (GRCm38)	nonsense	probably null
R2130:Atrnl1	UTSW	19	57,654,994 (GRCm38)	missense	probably damaging	1.00
R3710:Atrnl1	UTSW	19	57,657,114 (GRCm38)	missense	probably damaging	1.00
R3916:Atrnl1	UTSW	19	57,935,652 (GRCm38)	missense	possibly damaging	0.82
R4524:Atrnl1	UTSW	19	57,630,306 (GRCm38)	missense	probably damaging	1.00
R4707:Atrnl1	UTSW	19	57,629,158 (GRCm38)	missense	probably damaging	0.97
R4712:Atrnl1	UTSW	19	57,652,950 (GRCm38)	missense	probably benign	0.00
R4784:Atrnl1	UTSW	19	57,629,158 (GRCm38)	missense	probably damaging	0.97
R4785:Atrnl1	UTSW	19	57,629,158 (GRCm38)	missense	probably damaging	0.97
R4798:Atrnl1	UTSW	19	58,042,361 (GRCm38)	missense	probably benign
R5172:Atrnl1	UTSW	19	57,685,513 (GRCm38)	nonsense	probably null
R5226:Atrnl1	UTSW	19	57,650,335 (GRCm38)	missense	probably benign
R5289:Atrnl1	UTSW	19	57,657,082 (GRCm38)	missense	probably damaging	1.00
R5372:Atrnl1	UTSW	19	57,755,536 (GRCm38)	missense	probably benign
R5737:Atrnl1	UTSW	19	57,777,888 (GRCm38)	missense	possibly damaging	0.84
R5782:Atrnl1	UTSW	19	57,753,286 (GRCm38)	missense	possibly damaging	0.95
R5826:Atrnl1	UTSW	19	57,630,292 (GRCm38)	nonsense	probably null
R6169:Atrnl1	UTSW	19	57,642,463 (GRCm38)	missense	probably benign	0.00
R6242:Atrnl1	UTSW	19	57,642,478 (GRCm38)	missense	probably benign	0.02
R6342:Atrnl1	UTSW	19	57,638,510 (GRCm38)	missense	probably damaging	1.00
R6372:Atrnl1	UTSW	19	57,650,332 (GRCm38)	missense	probably benign	0.01
R6811:Atrnl1	UTSW	19	57,654,961 (GRCm38)	missense	probably damaging	0.98
R6897:Atrnl1	UTSW	19	58,042,368 (GRCm38)	missense	probably benign	0.01
R7024:Atrnl1	UTSW	19	57,638,450 (GRCm38)	critical splice acceptor site	probably null
R7085:Atrnl1	UTSW	19	57,691,857 (GRCm38)	missense	probably damaging	1.00
R7144:Atrnl1	UTSW	19	58,042,352 (GRCm38)	missense	probably damaging	1.00
R7259:Atrnl1	UTSW	19	57,935,606 (GRCm38)	nonsense	probably null
R7289:Atrnl1	UTSW	19	57,650,414 (GRCm38)	missense	probably benign	0.13
R7310:Atrnl1	UTSW	19	57,642,424 (GRCm38)	missense	possibly damaging	0.69
R7372:Atrnl1	UTSW	19	57,935,646 (GRCm38)	missense	possibly damaging	0.47
R7432:Atrnl1	UTSW	19	57,755,524 (GRCm38)	missense	probably damaging	1.00
R7478:Atrnl1	UTSW	19	57,696,312 (GRCm38)	missense	possibly damaging	0.89
R7556:Atrnl1	UTSW	19	57,654,846 (GRCm38)	missense	probably benign
R7567:Atrnl1	UTSW	19	57,699,523 (GRCm38)	missense	probably damaging	0.98
R7608:Atrnl1	UTSW	19	57,714,687 (GRCm38)	missense	probably damaging	1.00
R7632:Atrnl1	UTSW	19	57,630,306 (GRCm38)	missense	probably damaging	1.00
R7655:Atrnl1	UTSW	19	57,611,379 (GRCm38)	nonsense	probably null
R7656:Atrnl1	UTSW	19	57,611,379 (GRCm38)	nonsense	probably null
R7718:Atrnl1	UTSW	19	57,740,183 (GRCm38)	nonsense	probably null
R7721:Atrnl1	UTSW	19	57,696,331 (GRCm38)	missense	probably benign	0.00
R7726:Atrnl1	UTSW	19	57,702,072 (GRCm38)	missense	probably damaging	1.00
R7733:Atrnl1	UTSW	19	57,701,988 (GRCm38)	missense	probably benign	0.00
R7774:Atrnl1	UTSW	19	57,699,671 (GRCm38)	missense	probably damaging	1.00
R8119:Atrnl1	UTSW	19	57,642,463 (GRCm38)	missense	probably benign	0.00
R9242:Atrnl1	UTSW	19	57,657,228 (GRCm38)	missense	probably benign	0.07
R9265:Atrnl1	UTSW	19	57,777,927 (GRCm38)	missense	probably benign	0.11
R9272:Atrnl1	UTSW	19	57,654,988 (GRCm38)	missense	probably benign	0.00
R9480:Atrnl1	UTSW	19	57,701,988 (GRCm38)	missense	possibly damaging	0.61
R9526:Atrnl1	UTSW	19	57,629,119 (GRCm38)	missense	probably damaging	0.99
R9672:Atrnl1	UTSW	19	57,630,263 (GRCm38)	missense	possibly damaging	0.87
R9673:Atrnl1	UTSW	19	57,611,354 (GRCm38)	start codon destroyed	probably null	0.04
RF021:Atrnl1	UTSW	19	57,642,473 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AATTGATAGCGTTTATAGGTGTGCC -3'
(R):5'- TCGGCACCTTAATCATGGAGG -3'

Sequencing Primer
(F):5'- CCGAGTAGTGGCATGCATG -3'
(R):5'- TCGGCACCTTAATCATGGAGGATATG -3'

Posted On

2020-01-23