Mutagenetix > Incidental Mutation

Incidental Mutation 'R0678:Vmn2r8'

61706

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

038863-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R0678 (G1)

Quality Score

162

Status

Not validated

Chromosome

Chromosomal Location

108945059-108956620 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 108948412 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 492 (H492L)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

probably benign
Transcript: ENSMUST00000172140
AA Change: H492L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: H492L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 94.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 21 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Egfl7	C	T	2: 26,480,952 (GRCm39)	R155C	probably benign	Het
Fat4	T	C	3: 38,943,843 (GRCm39)	I912T	probably damaging	Het
H2-M5	A	G	17: 37,300,034 (GRCm39)	F47L	possibly damaging	Het
H2-T24	A	T	17: 36,328,333 (GRCm39)	F50Y	probably damaging	Het
Hif1a	A	G	12: 73,990,965 (GRCm39)		probably null	Het
Ints11	T	C	4: 155,972,210 (GRCm39)	I405T	probably damaging	Het
Kmt2d	A	T	15: 98,748,294 (GRCm39)		probably benign	Het
Pld1	T	A	3: 28,174,933 (GRCm39)	V857D	probably damaging	Het
Ptgr3	A	G	18: 84,113,287 (GRCm39)	E321G	probably benign	Het
Rab11fip3	G	A	17: 26,287,821 (GRCm39)	P111S	probably benign	Het
Rad17	A	T	13: 100,781,692 (GRCm39)	I35N	possibly damaging	Het
Rnf31	C	A	14: 55,839,170 (GRCm39)	Y66*	probably null	Het
Sec31a	T	C	5: 100,555,084 (GRCm39)	I45M	possibly damaging	Het
Sele	T	C	1: 163,882,298 (GRCm39)		probably null	Het
Serpina3i	G	T	12: 104,232,978 (GRCm39)		probably null	Het
Sp6	T	A	11: 96,912,607 (GRCm39)	W107R	probably damaging	Het
Sphkap	A	T	1: 83,256,349 (GRCm39)	W467R	probably benign	Het
Thbs1	T	C	2: 117,953,387 (GRCm39)	F935L	probably damaging	Het
Xrcc2	A	G	5: 25,903,261 (GRCm39)	S37P	possibly damaging	Het
Zfand1	T	A	3: 10,413,577 (GRCm39)	I31L	probably benign	Het
Zfr	G	A	15: 12,184,171 (GRCm39)	D1058N	probably damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108,950,091 (GRCm39)	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108,945,807 (GRCm39)	splice site	probably null
R0335:Vmn2r8	UTSW	5	108,945,317 (GRCm39)	splice site	probably null
R0394:Vmn2r8	UTSW	5	108,949,938 (GRCm39)	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108,951,049 (GRCm39)	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108,947,195 (GRCm39)	missense	probably damaging	1.00
R1167:Vmn2r8	UTSW	5	108,951,042 (GRCm39)	missense	probably benign	0.01
R1187:Vmn2r8	UTSW	5	108,951,085 (GRCm39)	nonsense	probably null
R1406:Vmn2r8	UTSW	5	108,950,234 (GRCm39)	missense	probably benign
R1406:Vmn2r8	UTSW	5	108,950,234 (GRCm39)	missense	probably benign
R1451:Vmn2r8	UTSW	5	108,945,933 (GRCm39)	missense	probably damaging	1.00
R1535:Vmn2r8	UTSW	5	108,950,040 (GRCm39)	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108,950,972 (GRCm39)	missense	probably benign
R1874:Vmn2r8	UTSW	5	108,950,284 (GRCm39)	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108,945,436 (GRCm39)	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108,950,019 (GRCm39)	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108,947,152 (GRCm39)	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108,945,961 (GRCm39)	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108,950,249 (GRCm39)	missense	probably benign	0.01
R2095:Vmn2r8	UTSW	5	108,956,487 (GRCm39)	missense	possibly damaging	0.94
R2159:Vmn2r8	UTSW	5	108,950,169 (GRCm39)	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108,945,369 (GRCm39)	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108,949,570 (GRCm39)	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108,956,447 (GRCm39)	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108,949,566 (GRCm39)	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108,945,264 (GRCm39)	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108,947,129 (GRCm39)	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108,956,572 (GRCm39)	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108,949,636 (GRCm39)	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108,950,106 (GRCm39)	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108,950,325 (GRCm39)	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108,945,248 (GRCm39)	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108,947,211 (GRCm39)	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108,956,463 (GRCm39)	missense	probably benign
R6315:Vmn2r8	UTSW	5	108,949,757 (GRCm39)	missense	probably benign
R6413:Vmn2r8	UTSW	5	108,949,589 (GRCm39)	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108,956,504 (GRCm39)	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108,948,442 (GRCm39)	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108,956,449 (GRCm39)	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108,945,522 (GRCm39)	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108,950,106 (GRCm39)	missense	probably benign	0.22
R7534:Vmn2r8	UTSW	5	108,950,040 (GRCm39)	missense	possibly damaging	0.94
R7739:Vmn2r8	UTSW	5	108,950,043 (GRCm39)	missense	probably damaging	1.00
R8099:Vmn2r8	UTSW	5	108,949,700 (GRCm39)	missense	probably benign
R8245:Vmn2r8	UTSW	5	108,945,936 (GRCm39)	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108,945,962 (GRCm39)	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108,945,597 (GRCm39)	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108,956,617 (GRCm39)	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108,950,131 (GRCm39)	missense
R8928:Vmn2r8	UTSW	5	108,950,131 (GRCm39)	missense
R9288:Vmn2r8	UTSW	5	108,950,185 (GRCm39)	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108,947,196 (GRCm39)	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108,951,107 (GRCm39)	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108,949,864 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- cacacacacacacacCGAGCTATAA -3'
(R):5'- GGCTTCCATGCTGAAATCCAGGA -3'

Sequencing Primer
(F):5'- tgggtgaataggcatgtgg -3'
(R):5'- CCATGCTGAAATCCAGGATATTTAC -3'

Posted On

2013-07-30